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Turning the focus knobs recklessly is a long and hard way to focus on the image. If you are using
a high power objective, you may even damage the specimen by pressing it against the objective.
Before breaking the coverglasses or damaging the objectives, read the following and find the
correct way to focus on the specimen.
(1) Put the 10x (or 4x) objective in the optical path.
(2) Turn the coarse focus knob to raise the stage to its upper limit.
(3) Looking into the eyepieces, slowly rotate the coarse focus knob to lower the
stage. When the specimen image appears, stop rotating the knob.
(4) Rotate the fine focus knob and precisely focus on the image.
When you want to observe the image with a high power objective, first focus
on the image using a 10x (or 4x) objective. Then change to a high power
objective and rotate the fine focus knob for precise focusing.
1. When rotating the coarse focus knob while looking into the
eyepieces, be sure to turn it only in the direction that lowers the
stage.
2. When raising the stage using the coarse focus knob, take your eyes
off the eyepieces and look at the gap between the upper surface of
the specimen and the front of the objective from the side.
3. First focus with a low power objective. Then change to a high power
objective.
*1:
Since the working distances of 10x and 4x objectives are large (p. 75), these objectives do not
touch the specimen even when the stage is raised to its upper limit provided that the slide and
coverglasses of a standard thickness are used. (The standard thickness for slides is 1.2 mm and
that for coverglass is 0.17 mm.)
Chapter 4 Microscopy (Detailed Procedure)
Focusing
First raise the stage...
56
*1
then look into
the eyepieces
and lower the
stage.
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