Leica DM750 M Manual
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Leica DM750 M Manual

Leica DM750 M Manual

Leica dm750 m
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Leica DM750 M
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  • Page 1 Leica DM750 M Manual...

  • Page 2: Table Of Contents

    Complete Condenser Centering (cont.) Assembling the Viewing Tubes Using the Condenser Leica EZ Viewing Tube with Integrated Eyepieces Preparation for Viewing a Specimen Slide Leica EZ Viewing Tube – integrated eyepieces (cont.) Focusing Leica Standard Tube with Separate Eyepieces Viewing Tube Adjustment Eyecups Viewing Tube Adjustment (cont.)

  • Page 3: Safety Instructions

    Safety instructions Leica DM750 M Manual...

  • Page 4: Safety Notes

    The "Safety Concept" brochure contains additi- Contact address ment. onal safety information regarding service work, Leica Microsystems (Schweiz) AG requirements and the handling of the micro- Industry Division scope, the electrical and other accessories as Max Schmidheiny Strasse 201 well as general safety instructions.

  • Page 5: Instructions On Use

    Instructions on use Intended use Interference Handling electrical components   The DM series microscopes by Leica are opti- Avoid large temperature fluctuations, direct Never install any other plug (NEMA cal instruments for improving the visibility of sunlight and vibrations. These conditions...

  • Page 6: Instructions On Use (Continued)

    Unless otherwise specified in this conclude a maintenance contract (recommen- manual, repair and service work may ded). be carried out by authorized Leica technicians only.   Accessories Unauthorized alterations to the instru- Only those accessories may be used that are...

  • Page 7: Assembling The Leica Dm750 M

    Assembling the Leica DM750 M Leica DM750 M Manual...

  • Page 8: Assembling The Incident Light Axis

    3. Fasten the incident light axis into place by 1. Place the incident light axis with the heat tightening the setscrew. sink facing backwards onto the tripod mount. 2. Align the incident light axis according to the tripod geometry. Leica DM750 M Manual...

  • Page 9: Assembling The Viewing Tubes

    Allen key provided. the tube automatically shifts to the correct position on the optical axis of the micro- scope. Type 1: Leica EZ Viewing Tube with integrated eyepieces Type 2: Standard tube with separate eyepieces Leica DM750 M...

  • Page 10: Leica Ez Viewing Tube With Integrated Eyepieces

    Leica EZ Viewing Tube with Integrated Eyepieces 1. In order to use the wingscrew, remove the 3. Reinstall the Leica EZ Viewing Tube onto setscrew that was delivered with the stand. the stand. Leica EZ Viewing Tube with integrated eyepieces  ...

  • Page 11: Leica Ez Viewing Tube - Integrated Eyepieces (Cont.)

    Leica EZ Viewing Tube – integrated eyepieces (cont.)   Now, you can rotate the Leica EZ Viewing Tube by loosening the wingscrew, brin- ging the tube to the desired position and tigh- tening the wingscrew again.   The eyepieces are integrated into the Leica EZ Viewing Tube and preset;...

  • Page 12: Leica Standard Tube With Separate Eyepieces

    Leica Standard Tube with Separate Eyepieces 1. Insert the eyepieces into the tubes. 2. Secure the eyepieces in the tubes by tighte- ning the silver screws at the bottom. Standard tubes; tubes do not include eyepieces   The standard tube includes a rotatable dovetail.

  • Page 13: Eyecups

    In this case, continue with the light. "Use" section starting on page 17. If you have purchased your Leica DM750 M in individual components and not according to the standard configuration, continue with the "Installing Objectives" section on page 14.

  • Page 14: Installing Objectives

    Installing Objectives   When rotating the objective nosepi- ece, always use the knurled ring on the objective nosepiece. While rotating the objective nosepiece clock- wise, screw the objectives into the objective nosepiece. Start with the lowest magnification. Leica DM750 M Manual...

  • Page 15: Conversion To Incident Light Polarization

    1. Unscrew the screw and remove the cover. adapter. it will go. The opening for the polarizing adapter is now accessible. 4. Remove the cover of the analyzer. 2. Insert the polarizing adapter into the opening and tighten the screw. Leica DM750 M Manual...

  • Page 16: Use

    Leica DM750 M Manual...

  • Page 17: Switching On The Microscope

    Precautionary measures 2. Switch on the illumination by briefly pres- The brightness can be adjusted in 15   The light of the Leica DM750 M can be sing the power switch. increments. very bright. Therefore, only look into the eyepieces after you have switched on and...

  • Page 18: Modifying The Aperture Diaphragm

      The aperture diaphragm must be completely open for all oblique light illu- mination scenarios. Leica DM750 M Manual...

  • Page 19: Illumination Scenarios

    (oblique incident illumination). oblique illumination. Combinations with other light fields are also possible.   The aperture diaphragm must be completely open for all oblique light illu- mination scenarios. HOLD Bright field illumination Individual light field Oblique illumination Leica DM750 M Manual...

  • Page 20: Illumination Scenarios (Continued)

    HOLD Any arrangement Switching between bright field illumination Switching automatically and the most recent setting between the light fields Leica DM750 M Manual...

  • Page 21: Using Specimen Holders

    Order number for 25 mm version: 13 613 Ϙ Order number for 30 mm version: 13 613 Ϙ 5. Position the specimen by moving the stage   The maximum height of the specimen is and looking through the eyepieces simulta- 30  mm. neously. Leica DM750 M Manual...

  • Page 22: Automatic Shutoff Of Illumination

    Automatic Shutoff of Illumination   The illumination of the Leica DM750 M Deactivating delayed shutoff Activating delayed shutoff is shut off automatically if the user does 1. Switch the incident light axis off. 1. Switch the incident light axis off.

  • Page 23: Conversion And Use With Transmitted Light

    Conversion and Use with Transmitted Light Leica DM750 M Manual...

  • Page 24: Optional Koehler Illumination

    Optional Koehler Illumination Transmitted illumination Type 1: Standard condenser Type 2: Koehler condenser   For the Leica DM750 M, there are two Adjustable condenser centering with tool kept Adjustable condenser centering with condensers available for optimum trans- at the ready: wingscrews and adjustable Koehler field mitted illumination.

  • Page 25: Installing The Condenser

    1. Move the specimen stage upwards as far as 3. Unscrew the two setscrews (or wingscrews   The Leica DM750 M is equipped with it will go using the coarse focusing knob. for Koehler stands) on the condenser an open holder for the specimen stage holder.

  • Page 26: Installing The Condenser (Continued)

      How to center the condenser more precisely is described in the section entitled "Complete Condenser Centering" on page 28. Leica DM750 M Manual...

  • Page 27: Switching On The Microscope

    Precautionary measures Connecting and switching on the micro- 3. Switch on the microscope using the switch   The light of the Leica DM750 M can be scope at the bottom right of the microscope very bright. Therefore, do not look into 1.

  • Page 28: Complete Condenser Centering

    Make sure that the condenser is in the 3. Look through the eyepieces and center the highest position. "X" in the field of view by tightening the setscrews. (Leica DM750 M with Koehler Centered illumination: tighten the knurled screws). Leica DM750 M...

  • Page 29: Complete Condenser Centering (Cont.)

    Complete Condenser Centering (cont.)   4. For a Leica DM750 M with standard conden- For a Leica DM750 M with Koehler field ser (without Koehler field diaphragm), you diaphragm, center and focus the conden- can now lock the condenser into its posi- ser more precisely.

  • Page 30: Using The Condenser

    The line on the ring is aligned with the objective magnification used. Leica DM750 M Manual...

  • Page 31: Preparation For Viewing A Specimen Slide

    1. Position a specimen slide on the specimen 2. Position the specimen slide such that a stage by sliding it under the slide grips. part of the specimen is under the objective used.   Slide grips hold the slide in place. Slide grips Leica DM750 M Manual...

  • Page 32: Focusing

      tion. The stand of the Leica DM750 M has been calibrated at the factory so that the focus can be found from this position within 1.5 rota- tions of the fine focus.

  • Page 33: Viewing Tube Adjustment

    Adjust the eyetubes Leica EZ Viewing Tube Standard Viewing Tube with Two Fixed 1. Adjust the tubes to your interpupillary If you are using a Leica EZ Viewing Tube, Eyepieces Ϙ distance. Fold or unfold the eyetubes to which has eyepieces that are integrated If you are using a standard tube with two Ϙ...

  • Page 34: Viewing Tube Adjustment (Cont.)

      If you are comfortable wearing your non-focusable eyepiece, look through and left eye. corrective lenses (contact lenses or the non-focusable eyepiece. eyeglasses) for microscope viewing, leave them on and your adjustments will be minimal. Leica DM750 M Manual...

  • Page 35: Viewing Tube Adjustment (Cont.)

    If your DM750 M is equipped with a Koeh- Ϙ ler configuration, continue with the next section entitled "Koehler Configuration" on page 36.

  • Page 36: Koehler Configuration

    Koehler Configuration   If your Leica DM750 M is equipped with a field diaphragm for Koeh- 2. Focus the illuminated field diaphragm using the condenser focusing ler illumination, center the condenser as described below. knob on the left side of the stage mount.

  • Page 37: Koehler Configuration (Cont.)

    Koehler Configuration (cont.) 3. Turn the condenser centering wingscrews simultaneously to center 4. Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm. of the field of view. Leica DM750 M Manual...

  • Page 38: Automatic Shutoff Of Illumination

    Automatic Shutoff of Illumination   The illumination of the Leica DM750 M Deactivating delayed shutoff Activating delayed shutoff is shut off automatically if the user does 1. Switch the incident light axis off. 1. Switch the incident light axis off.

  • Page 39: Care And Repair Instructions

    Care and Repair Instructions Leica DM750 M Manual...

  • Page 40: Care Instructions

    Do not store chemicals in the vicinity. bellows, or by using a vacuum. Remove coarse debris with a moistened Ϙ Protect the Leica DM750 M from oil and disposable cloth. Remove dirt using a clean cloth that has Ϙ...

  • Page 41: Accessories, Maintenance And Repair

    Accessories, Maintenance and Repair Accessories Maintenance Service address Only the following accessories may be used The Leica DM750 M is basically mainte- In case of problems, please contact us as Ϙ with the Leica DM750 M: nance-free. To ensure that it always opera-...

  • Page 42: Specifications And Dimensions

    Specifications and Dimensions Leica DM750 M Manual...

  • Page 43: Electrical Data And Ambient Conditions

      Unplug the instrument before changing any fuses. The Temperature for use +10 °C … +40 °C Leica DM750 M includes two fuses that are located behind Storage temperature -20 °C … +52 °C the power cable compartment. +50 °F … +104 °F  ...

  • Page 44: Dimensions (In Mm)

    Dimensions (in mm) Leica DM750 M Manual...

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