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Leica DM750 P
User Manual

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Summary of Contents for Leica DM750 P

  • Page 1 Leica DM750 P User Manual...
  • Page 2 Manufacturer Information Published in January 2015 by: Leica Microsystems (Schweiz) AG Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) In case of questions, please contact the hotline: sms.support@leica-microsystems.com Leica DM750 P User Manual...
  • Page 3: Table Of Contents

    Chapter Overview Safety regulations The Leica DM750 P Get Ready! Get Set! Care of the Microscope Dimensions Leica DM750 P User Manual...
  • Page 4 Time Delay Shutoff The Leica DM750 P Introduction Ready! Set! Go! Unpacking Care of the Microscope Get Ready! General Maintenance Attaching Analyzer Module or Analyzer/Bertrand Lens Module Dimensions Viewing Tube Dimensions Installing Objectives Substage Condenser Leica DM750 P User Manual...
  • Page 5: Safety Regulations

    Safety Regulations Leica DM750 P User Manual Safety Regulations...
  • Page 6: Safety Concept

    Safety Concept The individual modules of the Leica DM micros- Before installing, operating or using the instru- copy series include an interactive CD-ROM with ments, read the user manuals listed above. In all relevant user manuals in several languages. particular, please observe all safety instructions.
  • Page 7: Symbols Used In This Instruction Manual

    Instructions for disposing of the Failure to comply can cause the following: instrument, accessory components, Hazards to personnel and consumables. Functional disturbances or damaged instruments Leica DM750 P User Manual Safety Regulations...
  • Page 8: Important Notes

    Important Notes Description User manual Accessories from third-party suppliers The Leica DM750 P microscope meets today's This user manual includes important instruc- You can combine individual system articles with state of the art of technology. Nevertheless, tions related to operating safety, maintenance articles from external suppliers.
  • Page 9: Instructions On Use

    If using the devices outdoors, protect The Leica DM750 P microscope can be used in them from dust and moisture. Never use electri-  ...
  • Page 10 Once the product has reached the end of its When installing Leica products into third-party shipping or transporting individual modules. service life, please contact Leica Service or Sales products, the manufacturer of the complete about disposal. system or its dealer is responsible for follow-...
  • Page 11: Health Risks And Dangers Of Use

      Workplaces with microscopes facilitate Leica microscopy series are intended to reduce The Leica DM750 P microscope may only be and improve the viewing task, but they the exertion of the user to a minimum. connected to a grounded socket.
  • Page 12: Information For The Person Responsible For The Instrument

    Information for the Person Responsible for the Instrument Information for the person responsible for Do not use the Leica DM750 P microscope Only original Leica replacement parts may the instrument unless it is in perfect condition. be used in servicing the product.
  • Page 13: Care Instructions

    Remove coarse debris with a moistened disposable cloth. Optical surfaces should be cleaned with a Protect the Leica DM750 P microscope from lint-free cloth, lens tissue, or cotton swab oil and grease. Never grease or oil mechani- Acetone, xylene or nitro-containing thin- moistened with a commercially available cal parts or sliding surfaces.
  • Page 14: Accessories, Maintenance And Repair

    Accessories Maintenance Repairs and service work Only the following accessories may be used The Leica DM750 P microscope is basically Only original Leica Microsystems spare with the Leica DM750 P microscope: maintenance-free. To ensure that it always parts may be used.
  • Page 15: Electrical Data And Ambient Conditions

      Unplug the instrument before changing any fuses. The Temperature for use +10 °C … +40 °C Leica DM750 P has two fuses, which are located behind the Storage temperature -20 °C … +52 °C power cord receptacle. +50 °F … +104 °F...
  • Page 16: The Leica Dm750 P

    The Leica DM750 P Leica DM750 P User Manual The Leica DM750 P...
  • Page 17: Introduction

    Introduction Thank you for purchasing the Leica DM750 P Polarizing Microscope System from Leica Microsystems. The Leica DM750 P is a highly adaptable instrument with superior optics and precision engineering for educational and basic industrial polarized light microscopy applica- tions.
  • Page 18: Unpacking

    These items are delivered in separate packages. Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM750 P User Manual The Leica DM750 P...
  • Page 19: Get Ready

    Get Ready! Leica DM750 P User Manual Get Ready!
  • Page 20: Attaching Analyzer Module Or Analyzer/Bertrand Lens Module

    Allen key 1. Slightly unscrew the set screw located on top of the stand with the Allen key tool provided. 3. Insert the dovetail of the module into the stand. (Analyzer/Bertrand Lens Module shown). Leica DM750 P User Manual Get Ready!
  • Page 21: Viewing Tube

    4. Insert the eyepieces into the eyetubes. ing tube into the module and tighten the set screw firmly without forcing it. This precisely positions the body onto the opti- cal axis of the microscope. Leica DM750 P User Manual Get Ready!
  • Page 22: Installing Objectives

    In this case, go to highest magnification. section "Operation". If you purchased your Leica DM750 P by components and not by standard configuration, please continue in this section. •  Always rotate the nosepiece by using the knurled nosepiece ring.
  • Page 23: Substage Condenser

    Substage condenser 2. Adjust the condenser mount to its lowest the bottom of the condenser mount, to the   The Leica DM750 P has an open sub- position using the condenser focus knob left. stage condenser mount. Therefore, the on the left-hand side of the substage.
  • Page 24 Koehler Setup. 5. Slide the substage condenser into the fork mount located underneath the stage by aligning the positioning pin on the condenser into the slot on the back of the fork. Leica DM750 P User Manual Get Ready!
  • Page 25: Get Set

    Get Set! Leica DM750 P User Manual Get Set!
  • Page 26: Basic Setup

    3. Set the illumination control (knob is located has been grounded to the microscope The DM750 P has a 5 V/1.5 A USB power connec- on the bottom left-hand side of the micro- base. Never use an adapter between the power tor in the center of the cord wrap.
  • Page 27: Using The Aperture Condenser

    2. To start, fully open the iris diaphragm of the aperture condenser by rotating the ring to the extreme right. Leica DM750 P User Manual Get Set!
  • Page 28: Setting Up Polarized Light

    2. Rotate the polarizer to the zero position. substage condenser in a swing in/out which is located on the right-hand side of mount. Swing it into place and be sure it "clicks" the polarizer. into place. Leica DM750 P User Manual Get Set!
  • Page 29 Analyzer/ look dark. If not, rotate the polarizer slightly Bertrand Lens Module is flipped to the left until you have the darkest field of view. (the "O" position/Bertrand Lens out). Leica DM750 P User Manual Get Set!
  • Page 30: Focusing

    2. Rotate the 10× objective into working posi- 5. Using the fine focus adjustment knob, tion. bring the specimen into sharp focus. Leica DM750 P User Manual Get Set!
  • Page 31: Viewing Tube Adjustment

    (focusing eyepiece without cross- in the slot of the right eyetube. slightly, if at all. hair) with the other eye. Leica DM750 P User Manual Get Set!
  • Page 32: Koehler Set Up

    2. Use the condenser focus knob on the left-hand side of the substage to 4. Open the field diaphragm until the iris leaves disappear just beyond bring the leaves of the field diaphragm into sharp focus. the field of view. Leica DM750 P User Manual Get Set!
  • Page 33: Objective Centration

    This may even be outside of the field of view. This indicates the objec- tive is not centered to the stage. Leica DM750 P User Manual Get Set!
  • Page 34   Each objective must be centered sepa- rately. Also, there is a magnetic storage location for the objective centering tools in the back of the Analyzer or Analyzer/Bertrand Lens modules. Leica DM750 P User Manual Get Set!
  • Page 35: Bertrand Lens Operation

    Bertrand Lens) to the center of the field 2. Swing in the Bertrand Lens by flipping the upper rocker of the Analyzer/Bertrand Lens module to the "B" position. Be sure the Polarizer and Analyzer are also in position. Leica DM750 P User Manual Get Set!
  • Page 36: Time Delay Shutoff

    Time Delay Shutoff   The Leica DM 750 P is equipped with Changing the status of the time delay The LED Illumination will flash to indicate a time delay shutoff capability, which shutoff the time delay shutoff status was changed.
  • Page 37 Leica DM750 P User Manual...
  • Page 38: Ready! Set! Go

    Ready! Set! Go! Now all you need to do is change objectives, set the aperture condenser and field diaphragm appropriately for the objective magnification in use, and enjoy the view! Leica DM750 P User Manual...
  • Page 39: Care Of The Microscope

    Care of the Microscope Leica DM750 P User Manual Care of the Microscope...
  • Page 40: General Maintenance

    Keep all optical components clean. Clean- around the lens. liness is important for maintaining good optical performance. Leica DM750 P User Manual Care of the Microscope...
  • Page 41 Note: To achieve the high degree Illumination of flatness obtained with higher magnifica- The Leica DM750 P uses LED illumination. tion objectives, the objective has a small Therefore, no lamp changing is required for concave front lens of fairly short radius or the life of the microscope.
  • Page 42: Dimensions

    Dimensions Leica DM750 P User Manual Dimensions...
  • Page 43: Dimensions

    Leica DM750 P User Manual Dimensions...
  • Page 44 Leica DM750 P User Manual Dimensions...
  • Page 45 Leica DM750 P User Manual Dimensions...

Table of Contents