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• • • •
Specifications of the condenser lenses
N.A.
Working distance
Available microscopy
Usable condenser modules
Others
9. Objectives
Ph objectives
A Ph objective is labeled with a Ph code: PhL, Ph1, Ph2 or Ph3.
For Ph microscopy, use the annular diaphragm (condenser module) that has the same Ph code as the objective, regardless
of the magnification of the objective.
Objective with correction ring
An inverted microscope is frequently used to observe specimens through the bottom plate of a laboratory dish or culture
bottle made of glass or plastic. For such applications, the normal objectives (for 0.17 mm thick cover glass) may not
provide clear images, disabling the microscope from demonstrating its full performances. In such cases, use an objective
with a correction ring to compensate for bottom plate thickness.
Since the objectives with correction rings are not intended to compensate for wedge-like changes of thickness at edges of
a container, we recommend that they should be used for compensation for even thickness.
• • • •
Adjusting the correction ring
1)
Turn the correction ring to match the bottom plate thickness of the container. The thickness should be a
measured value or the value stated by the manufacturer of the container. (The acrylic stage ring is available to
improve working efficiency since it enables the part of operation to be seen from above the stage.)
2)
Focus on the specimen with the focus knobs.
3)
If the image has poor resolution and/or contrast, slightly rotate the correction ring clockwise or counterclockwise.
When the correction ring is rotated, the specimen image becomes slightly out of focus. Focus on the specimen
again with the fine focus knob.
4)
If this has improved the resolution and contrast, rotate the correction ring further in the same direction, then
adjust the focus again.
If the resolution and contrast are deteriorated, rotate the correction ring in the reverse direction double the
amount rotated in previous turn, then adjust the focus.
In this way, rotate the correction ring in the same direction if a better image is obtained , or rotate it in this reverse
direction if a poor image is obtained. Repeat this operation to find the best point.
5)
Taking a note of the reading of a well-visible position on the correction ring will be a help when you later use
containers having different bottom plate thickness. (The 0 mm position of the correction ring is used for
microscopy of a specimen with no cover glass on an upright type microscopes.)
LWD condenser lens
0.52
30 mm
BF, Ph, DIC
For BF:
"A"
For Ph:
"PhL", "Ph1", "Ph2", "Ph3"
For DIC:
"DIC L", "DIC M", "DIC H"
For HMC:
"MC1", "MC2", "MC3"
* Condenser refocusing clamp can be used.
* Supplementary lens is not used with this
microscope.
III. Operation of each part
ELWD condenser lens
0.3
75 mm
BF, Ph
For BF:
「A」
For Ph:
"PhL", "Ph1", "Ph2"
45
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