Oil Immersion Technique; Manual - Leica DM500 Manual

Oil Immersion Technique

Oil Immersion Technique
1. Find the field of view on the slide that you
would like to inspect.
2. Lower the stage to its lowest position using
the coarse focus knob.
Leica DM 500, DM500 B
3. Place a drop of Leica Immersion Oil on the
specimen slide on the area of the specimen
that you were inspecting.
4. Swing the oil immersion objective in place
(the objective will be labeled "OIL")

Manual

5. Raise the stage slowly using the coarse
focus knob until the drop of oil on the spec-
imen slide just comes in contact with the
front of the oil immersion objective lens.
6. Grip the knurled nosepiece ring and rock
the objective back and forth to eliminate
any air bubbles, then settle the oil objec-
tive into position with the oil between the
front lens of the objective and the speci-
men slide.
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