Though the reliability and robustness of Leica microscopes is already practically proverbial, they still require a certain degree of care and attention. Therefore, we recommend that you read this User Manual.
Unpacking Identifying your Tube Dimensions Mounting the binocular tube Dimensions (all dimensios in mm, DM100 and DM300 identical) Installing the Monocular Tube Dimensions (all dimensios in mm, DM100 and DM300 identical) Removing and Inserting Objectives Removing and inserting objectives (continued)
Failure to comply can cause the following: Hazards to personnel. Failure to comply can cause the following: Ϙ Hazards to personnel. Instrument malfunctions and damage. Ϙ Ϙ Instrument malfunctions and damage. Ϙ Leica DM300 User Manual...
Original Condition non-Leica components that are outside of the To maintain the unit in its original condition scope of this User Manual! Unauthorized alte- and to ensure safe operation, the user must...
Legal Regulations Disconnecting from the Power Supply Adhere to general and local regulations rela- Always position the Leica DM300 so ting to accident prevention and environmental that you can disconnect it from the protection. power supply at any time. The power cable...
The Leica DM300 can be used at +10 °C to +40 °C. Protect the microscope from oil, chemi- cals and extreme humidity. If it is used outdoors, the microscope must be protected from dust and moisture.
(according to the User Integration in third-party products Manual) can be assembled and disassembled When installing Leica products into third-party by the customer should be packed separately. products, the manufacturer of the complete system or its dealer is responsible for following...
Monitor Only original Leica replacement parts may Ϙ compliance with this. be used in servicing the product. Do not use the Leica DM300 unless it is in Ϙ perfect condition. Leica DM300 User Manual...
Accessories, Maintenance and Repair Accessories Maintenance Repairs and Service Work Only the following accessories may be used The Leica DM300 microscope is basically Only original Leica Microsystems spare Ϙ Ϙ with the Leica DM300: maintenance-free. To ensure that it always parts may be used.
In order to maintain this condition and to ensure safe operation, Unplug the instrument before changing any fuses. The Leica the user must follow the instructions and warnings contained in DM300 has two fuses, which are located behind the power cord this instruction manual. receptacle. ...
These items are delivered in separate packages. Please do not discard any of the packing Ϙ materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM300 User Manual...
Type 1: Monocular "EZTube" Type 2: Binocular "EZTube" Other tubes, however, are installed exactly the same way. Orientation All tubes are connected via a dovetail and can be installed in any orientation. Type 3: Open binocular tube Leica DM300 User Manual...
2. Insert the dovetail of the binocular tube into the mount. Eyepieces The eyepieces are already integrated in the Leica "EZTube" and preset; for this reason, the eyepieces do not require adjust- ment or assembly. Leica DM300 User Manual...
2. Insert the dovetail of the monocular tube into the mount. Eyepieces The eyepieces are already integrated in the Leica "EZTube" and preset; for this reason, the eyepieces do not require adjust- ment or assembly. Leica DM300 User Manual...
The objectives are already installed Always turn the objective nosepiece by The proper Sequence on the Leica DM300 at delivery. The its knurled ring. Never turn the objective When inserting the objectives, you can following description shows how to (un)install nosepiece by using an objective as a handle.
1. Screw the 4× objective into any available space on the objective nosepiece. 2. Turn the objective nosepiece clockwise one unit. 3. Screw in the next-largest objective. 4. Repeat steps 2 and 3 until all objectives have been installed. Leica DM300 User Manual...
The mirror is equipped with a flat and a concave surface. Try them out to see which side works better. 3. Look through the eyepieces and align the mirror so that it works best. Leica DM300 User Manual...
2. Place the analyzer with the flat side facing down into the beam path. The two screws have to rest in the two existing holes. 3. Install the tube. 5. Turn the polarizer to align it. The arrows indicate the current orientation. Leica DM300 User Manual...
2. Set the illumination to the lowest setting grounding ineffective. using the controller on the bottom left of the stand. Work Surface Always use the microscope on a hard, stable surface. Leica DM300 User Manual...
The condenser is furnished with an iris 1. Rotate the desired objective over the specimen stage so that the displayed value diaphragm, which has to be adjusted to sample. matches the selected objective. match the effective numerical aperture of the individual objectives. Leica DM300 User Manual...
2. Insert the specimen slide and slowly release 3. Using the X/Y control, position the speci- 1. Open the specimen holder using the the tension lever. men slide such that a part of the specimen tension lever. is under the objective used. Leica DM300 User Manual...
4. Bring the specimen into focus using the tive with the lowest magnification level is fine adjustment knob. turned into the working position. Leica DM300 User Manual...
1. Set the focusing eyetube (left side) to "0". tion. Leica EZ tubes If you are using a Leica EZ viewing tube, which already has the eyepieces integrated with the eyetubes, no additional adjustments are neces- sary. Be sure you are wearing your eyeglasses or contact lenses.
4. Rotate the knurled ring on the left eyetube, until the specimen is sharply focused for the left eye. This corrects for any vision differences between your right eye and left eye. Leica DM300 User Manual...
The risk can be order to help block out ambient room light. minimized by the use of individual eyecups. Eyecups can be ordered separately. Please contact your Leica partner. Leica DM300 User Manual...
Oil Immersion Technique Oil Immersion Technique 3. Add a drop of immersion oil from Leica 5. Slowly move the specimen stage upwards 1. Search for the area on the specimen slide to the area of the specimen slide you are using the coarse adjustment knob until that you want to study.
Follow the instructions of the disinfectant Ϙ surfaces or accessories with rubberized manufacturer. parts. This could damage the surfaces, and We recommend concluding a service specimens could be contaminated by abra- Ϙ contract with Leica Service. ded particles. Leica DM300 User Manual...
General Maintenance (cont.) Illumination The lower magnification objectives have Ϙ The Leica DM300 uses an LED illuminator. fairly large front lenses and can be cleaned Ϙ Therefore, no lamp changing is required for with a cloth or lens tissue wrapped around the entire life of the microscope.
Dimensions (all Dimensions in mm, DM100 and DM300 identical) side view with binocular tube front view with binocular tube side view with binocular tube 180° rotated Ϙ Ϙ Ϙ for storage Leica DM300 User Manual...
Dimensions (all Dimensions in mm, DM100 and DM300 identical) side view with monocular tube front view with monocular tube side view with monocular tube 180° Ϙ Ϙ Ϙ rotated for storage Leica DM300 User Manual...