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OPERATING MANUAL
CO
Incubator
2
WCI-40 / WCI-120 / WCI-180 / WCI-260
WCI-650 / WCI-850 / WCI-1200
WIGGENS GmbH
Gässlesweg 22-24, 75334 Straubenhardt,
Germany
Tel.: 0049 7248 4529088
Wiggens China
Room 426, Hall A, Office Building M8,
No.1 Jiuxianqiao East Road, Chaoyang
District, Beijing 100015, China
Tel: +86 400-809-2068
Fax: +86 400-809-2068-112
info@wiggens.com
www.wiggens.com
W6011/6012XXX-V3.2

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  • Page 1 English OPERATING MANUAL Incubator WCI-40 / WCI-120 / WCI-180 / WCI-260 WCI-650 / WCI-850 / WCI-1200 WIGGENS GmbH Gässlesweg 22-24, 75334 Straubenhardt, Germany Tel.: 0049 7248 4529088 Wiggens China Room 426, Hall A, Office Building M8, No.1 Jiuxianqiao East Road, Chaoyang...
  • Page 2: Unpacking And Inspecting

    Congratulations! You have made an excellent choice. WIGGENS thanks you for the trust you have placed in us. This operating manual has been designed to help you gain an understanding of the operation and possible applications of our instruments. For optimal utilization of all functions, we recommend that you thoroughly study this manual prior to beginning operation.
  • Page 3: Table Of Contents

    WIGGENS CO Incubator CONTENTS 1. Safety and general precaution ....................5 1.1. General information on precaution ................5 1.2. CE Conformity ........................ 5 1.3. Precaution for using the power cable ................6 1.4. Precaution for ground connection ................. 6 1.5. Precaution for use ......................7 2.
  • Page 4 Operating Manual 9.1. UV decontamination ....................24 9.2. Printer .......................... 26 10. Service and Check Point ......................26 11. Troubleshooting ........................27 12. Service ........................... 28 12.1. Warranty ........................28 12.2. Contact /Technical Service ..................28...
  • Page 5: Safety And General Precaution

    WIGGENS CO Incubator 1. Safety and general precaution 1.1. General information on precaution  Precaution is to prevent the possible accident or danger during operation. So, you must keep it.  Precaution is divided into caution and warning. And, each of them has following meanings.
  • Page 6: Precaution For Using The Power Cable

    Operating Manual 1.3. Precaution for using the power cable Do not make the power plug be pressed by back of the product. (A space between the product and the plug must be 30cm at least. The power outlet must be only for this product. (Using various products simultaneously can cause a fire) Clean the power plug with a dry towel and connect it properly.
  • Page 7: Precaution For Use

    WIGGENS CO Incubator 1.5. Precaution for use You must not disassemble, fix and remodel the product by yourself. (You can damage the product throughout a fire and malfunction or get a property loss as well as experimental loss. Do not use the product for different purpose.
  • Page 8: Transportation, Storage And Location Of Installation

    Operating Manual 2. Transportation, storage and location of installation 2.1. Transportation DO NOT try to slide or tilt the unit Lift the unit at its four lower corners with the aid of 2 people. (Weight: 35kg) Permissible ambient temperature range for transport: -10℃ to 60℃. 2.2.
  • Page 9: Location Of Installation And Ambient Conditions

    WIGGENS CO Incubator 2.3. Location of installation and ambient conditions Do not put inflammable substances near the product. (It can cause a fire) When you install the product, you have to put the distance of at least 30cm from the wall. To completely separate the unit from the power supply, power plug must be disconnected.
  • Page 10: Prerequisite And Configuration

    When you have received the instrument which is packed on pallet, inspect the box carefully for any damages that may have caused any damages to product during shipping. Please report any damage to the carrier or to your local WIGGENS distributor immediately. LOCATION The incubator is designed to operate at temperature 5℃...
  • Page 11: Configuration

    WIGGENS CO Incubator 3.2. Configuration Exterior Configuration of CO incubator Air jacket-6 side direct heating Outer door Ventilation fan Magnetic door seal Glass door heater-Prevent condensation Power switch Stainless steel shelve- Perforated to improve Control panel Inner door seal- Airtight to prevent CO...
  • Page 12: Features And Specifications Of The Unit

    Operating Manual 4. Features and specifications of the unit 4.1. Features 1. Excellent Uniformity of Temperature, CO 2. 6 Sides Direct Heat for Temperature Uniformity and Fast Recovery 3. IR CO Sensor detects precise density of CO 4. Outer Heated Door ensures no condensation on glass door. Max.
  • Page 13: Specification

    WIGGENS CO Incubator 4.2. Specification WCI-40 / WCI-120 / WCI-180 / WCI-260 Items WCI-40 WCI-120 WCI-180 WCI-260 Temperature Range Ambient +5 C to 60 Accuracy ±0.1 C at 37 Uniformity at 37 C / RT 25 ±0.3 ±0.3 ±0.3 ±0.4 Inlet pressure range 0.3~0.5bar...
  • Page 14 Operating Manual WCI-650 / WCI-850 / WCI-1200 Items WCI-650 WCI-850 WCI-1200* Temperature Range Ambient +5 C to 60 Accuracy ±0.1 C at 37 Uniformity at 37OC / RT 25 OC ±0.5 ±0.5 ±0.5 Inlet pressure range 0.7~1.0bar 0.7~1.0bar 0.7~1.0bar Controller Microprocessor Digital PID Humidity at 37 70% ~ 80% (with water tray)...
  • Page 15: Control Panel

    WIGGENS CO Incubator 5. Control Panel Heating Signal: To show status of heating activation at 3 parts where is controlled by 3 each sensor. Glass door means the heater around glass door. Temperature Display LED window. Temperature set button. Set temperature display.
  • Page 16: Operation

    Operating Manual 6. Operation Before Switch ON, insert water tray filling with distilled water (If necessary). Make sure connection of gas supply. And Open CO2 gas cylinder or supply with the pressure of regulator set to 4.5~7.25Psi or 0.3~0.5 bar. 6.1.
  • Page 17: Calibration Of Temperature & Co2

    WIGGENS CO Incubator 6.4. Calibration of temperature & CO Please follow up the procedure for calibration below in case of discrepancy between actual value (measured by reliable thermometer or device) in chamber and displayed value. Conduct the analysis of CO density and Temperature when incubator has been stabilized for more than 2 hours.
  • Page 18: Alarm

    Operating Manual Push UP (▲) and DOWN (▼) to adjust CO % display. Before release of CO incubator from WIGGENS factory, CO density is calibrated optimizing at 5%. When using different density ofCO rather than 5%, this calibration is highly recommendable.
  • Page 19: Hot Air Sterilization (Only For Sterilization Model, Serial No. Are W6012Xxx

    WIGGENS CO Incubator 6.7. Hot Air Sterilization (Only for Sterilization model, Serial No. are W6012xxx) When CO incubators are used in contamination hazardous areas, hot air sterilization can be used as an additional means of cleaning the inside of the chamber. Hot Air Decontamination uses 6 side direct heating up to 125°C. This makes chamber decontaminated destroying bacteria and germ.
  • Page 20 Operating Manual How to start Figure 1: Example of the display of an incubator with the hot air sterilization option. After conducting prerequisite, take the following steps: Turning on the cycle: On the display of the incubator, press the two following buttons simultaneously (∧& CAL/SET) which directed as CLEAN MODE.
  • Page 21: Safety Switch

    WIGGENS CO Incubator 7. Safety Switch It is the safety device to prevent the heater from overheating, when the temperature controller is malfunctioning. Set the Safety S/W higher than setting point.  The Safety S/W has wide deviation  Safety S/W is the safety device for preventing the heater to overheat when ...
  • Page 22: Rs-232 Interface

    Operating Manual 8.1. RS-232 interface The RS-232 interface uses automatic identification. Parameter settings are as follows: Baud: 4800~19200 bits/second Data bits: 8 Stop bits:1 Handshake: None Parity: None List of commands Item Input / output Content of "#" Remarks Set the equipment parameters Set temperature out_sp_00 #### Decimal number...
  • Page 23 WIGGENS CO Incubator Holding register Register address Explanation Data type Remark Read or write (DEC.) 0:4800 1:9600 40001 Baudrate Read and write 2:19200 3:38400 40002 Set temperature Unit:0.1℃ Read and write 40004 Set CO2 density Unit:0.1% Read and write 0: Null...
  • Page 24: Optional Function

    UV Lamp is located near circulation fan and above ceiling of chamber. It decontaminates circulation fan and in-air circulated from fan. Most light is blocked by ceiling. Circulation Fan UV Light (View when taking off ceiling of chamber, model WCI-40/120/180/260...
  • Page 25 When UV lighting with cover, expose of UV light to chamber is very little. However, it is not guaranteed that cell is not damaged in this situation. WIGGENS recommend avoid cell culture when UV light is on even with cover.
  • Page 26: Printer

    Operating Manual After the UV lighting without ceiling, ceiling need to be returned to its position. For that, follow below procedure. Insert it until it is reached more than the part at top which bended to inside. At that point, lift the edge of ceiling up and put two holes into its hook.
  • Page 27: Troubleshooting

    5x20mm, Unit does The miniature fuse has blown. 220V(5A),110V(7A). If the newly inserted fuse triggers switch on (main again, there is short circuit: contact WIGGENS service. switch position “l” Switch defective Replace the switch Defective function of the CO concentration Reset the alarm.
  • Page 28: Service

    WIGGENS reserves the right to decide the validity of any warranty claim. In case of faults arising either due to faulty materials or workmanship, parts will be repaired or replaced free of charge.
  • Page 29: Confirmation Of Condition Of Unit

    WIGGENS CO Incubator Confirmation of condition of unit In the case of repair, copy and complete the Conformation of condition of unit and send it to WIGGENS Instruments. 1. Details about the unit Product number Serial number Reason for repair 2.
  • Page 30 Operating Manual Phone E-mail...
  • Page 31 WIGGENS CO Incubator WIGGENS GmbH Gässlesweg 22-24, 75334 Straubenhardt, Germany Tel.: 0049 7248 4529088 WIGGENS China Room 303, Hall C, Office Building M8, No.1 Jiuxianqiao East Road, Chaoyang District, Beijing 100015, China Tel: +86 400-809-2068 Fax: +86 400-809-2068-112 info@ wiggens.com...

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