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OPERATING MANUAL
Heating Shaker
WS-350B
WIGGENS GmbH
Gässlesweg 22-24, 75334
Straubenhardt, Germany
Tel.: 0049 7248 4529088
WIGGENS China
Room 303, Hall C, Office Building M8,
No.1 Jiuxianqiao East Road,
Chaoyang District, Beijing 100015,
China
Tel: +86 400-809-2068
Fax: +86 400-809-2068-112
info@ wiggens.com
www.wiggens.com
English

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  • Page 1 English OPERATING MANUAL Heating Shaker WS-350B WIGGENS GmbH Gässlesweg 22-24, 75334 Straubenhardt, Germany Tel.: 0049 7248 4529088 WIGGENS China Room 303, Hall C, Office Building M8, No.1 Jiuxianqiao East Road, Chaoyang District, Beijing 100015, China Tel: +86 400-809-2068 Fax: +86 400-809-2068-112 info@ wiggens.com...
  • Page 2: Unpacking And Inspecting

    Congratulations! You have made an excellent choice. WIGGENS thanks you for the trust you have placed in us. This operating manual has been designed to help you gain an understanding of the operation and possible applications of our instruments. For optimal utilization of all functions, we recommend that you thoroughly study this manual prior to beginning operation.
  • Page 3: Table Of Contents

    WIGGENS Heating Shaker WS-350B Content 1. Safety .............................. 5 1.1. Operation Manual ........................ 5 1.2. WARNING/CAUTION/NOTICE Alerts ..................5 1.3. Exemption for Responsibility ....................5 1.4. Warning Statement ......................6 1.5. Caution Statement ....................... 7 2. Functional Description ........................8 2.1.
  • Page 4 4.3.3. Checking Remaining Time during Timer Operation ..........20 4.3.4. Stopping Timer during Operation ................21 4.4. Program Mode ........................21 4.4.1. Starting User Program ..................... 23 4.4.2. Display during Program Operation ................24 4.4.3. Editing User Program ....................25 4.4.4.
  • Page 5: Safety

    WIGGENS Heating Shaker WS-350B 1. Safety 1.1. Operation Manual This manual contains important safety and operation information. You must carefully read, understand, and follow all the instructions in this manual prior to operating this instrument. Keep this manual in a safe place nearby for reference and make it easily available to all users.
  • Page 6: Warning Statement

    1.4. Warning Statement Warning: Ignoring the following warnings could cause serious injuries or even fatal accidents. Ignoring the following warnings could cause serious injuries or even fatal accidents. Case of explosive and flammable chemicals, you must use with sufficient safety countermeasure. In accordance with experiment, you should install safety devices and should follow suitable regulations in your laboratory.
  • Page 7: Caution Statement

    WIGGENS Heating Shaker WS-350B 1.5. Caution Statement Caution: Indicates a hazardous situation which, if not avoided, may result in minor or moderate injury. Do not touch block immediately, even power off. Because the block might be have Residual heat. It may be cause burns from high temperature of block, seemingly invisible.
  • Page 8: Functional Description

    2. Functional Description 2.1. Introduction WS-350B is generally used in for mixing sample, homogenization process, separation of the DNA / Plasmid, gel elution, and DNA, RNA, Protein, Yeast denaturation. General application follows that: Lipid extractions  Denaturation of DNA, RNA and proteins ...
  • Page 9: Construction

    WIGGENS Heating Shaker WS-350B 2.3. Construction VFD (Vacuum Fluorescent Display) : To display operating status. (1.) Touch Button : To choose temperature and time (2.) Dial Knob : To set a temperature value and time (3.) Block cover (Included in purchasing a block option) (4.)...
  • Page 10: Installation

    Inspect to see that the instrument is not damaged during transportation. (3.) 3.2. Checking Instrument Components Please check the instrument components supplied in the package after unpacking. (1.) If a noticeable or an omission is found, immediately notify your local WIGGENS dealer’s Service (2.) Department. Item...
  • Page 11: Location Conditions

    WIGGENS Heating Shaker WS-350B 3.4. Location Conditions Place the instrument far from the other instruments and keep the proper distance (normally more than 30℃). Warning: Place and install the instrument on a stable fireproof surface with non-slip and non-moisture and avoid direct sunlight & heat.
  • Page 12: Instrument Plug-In

    3.7. Instrument Plug-in When you switch on, the instrument displays as below. Notice: Touch Buttons are displayed with green or red colored backlight. When a backlight  color of a button is green, the button is valid so that it can sense user’s touches to itself.
  • Page 13: Operation

    WIGGENS Heating Shaker WS-350B 4. Operation 4.1. Control Panel and Function VFD (Vacuum Fluorescent Display) : You can check set value of temperature and shaking speed(RPM) (1.) and current state of operation. v Rem’n Remaining time during operation Indicate heating control ON.
  • Page 14: General Modes

    Set timer function. TIMER Check remaining time or elapsed time. Dial Knob : It can be used when setting temperature, shaking speed, timer and program parameters. (3.) 4.2. General Modes 4.2.1. Setting Temperature Press TEMP when the temperature control stops. (1.)...
  • Page 15 WIGGENS Heating Shaker WS-350B Notice: To step back from temperature setting, press START/STOP. The instrument returns  to standby. You can also change the temperature SV when the instrument is in operation.[Refer  to 4.2.1-1 Changing Temperature in Operation] •The instrument keeps the temperature after the temperature PV(Process Value) reaches to its SV by repeating Heater on and off.
  • Page 16: Setting Shaking Speed

    4.2.1.2. Checking Elapsed Time in Operation Elapsed time for operation can be checked by pressing TIMER once when the instrument is in operation. [Elapsed time] To step back from the elapsed time check, press START/STOP or leave the instrument for 10 seconds. 4.2.1.3.
  • Page 17: Timer Modes

    WIGGENS Heating Shaker WS-350B 4.2.2.1. Stopping the Shaking Operation You can stop the shaking operation by turning Dial Knob left side to make RPM SV 00. The instrument stops shortly after setting SV to 00. 4.3. Timer Modes This instrument provides two types of timers(T1 and T2 timer). The timers are different in a operating condition and point of time countdown.
  • Page 18: Setting T1 Timer

    4.3.1. Setting T1 Timer T1 timer can be set only when the instrument stops temperature control. After setting T1 timer, the temperature control starts operating to make the temperature PV the same to the temperature SV. When its temperature reaches to the temperature SV, the timer starts to count down. Press TIMER when the temperature control is not operating.
  • Page 19: Setting T2 Timer

    WIGGENS Heating Shaker WS-350B Confirm the end of the timer operation by using Touch Buttons or Dial Knob. Notice: If you press Dial Knob in “RUN NO”, all inputs for T1 timer are canceled and the  instrument returns to the standby.
  • Page 20: Checking Remaining Time During Timer Operation

    When the timer operation ends, the instrument generates a sound alarm with a display as follows. (4) Confirm the end of the timer operation by using Touch Buttons or Dial Knob. Notice: In case of T2 timer, the timer starts to count down just after the timer setting, regardless of reaching the temperature SV.
  • Page 21: Stopping Timer During Operation

    WIGGENS Heating Shaker WS-350B 4.3.4. Stopping Timer during Operation You can stop the operation by pressing START/STOP. Confirm the end of the timer operation by using Touch Buttons or Dial Knob. Caution: The instrument and its accessories can be hot even though the Power Switch is off.
  • Page 22 ABBREVIATIONS DESCRIPTIONS PROG (PR) User program INTV T (IN) Interval mix program EXEC Run a program VIEW View configurations of a program EDIT Edit configurations of a program CLER Clear all programs or a program. [Temperature and shaking speed control in Program Mode] Notice: A time SV for a step starts to be counted just after the instrument temperature reaches to the temperature SV of the step.
  • Page 23: Starting User Program

    WIGGENS Heating Shaker WS-350B 4.4.1. Starting User Program Press the PROGRAM when the instrument is on standby. (1) Select one of user programs by turning Dial Knob to the right side. (2) Turn dial Push dial Select a program execution “PR n EXEC” by pushing Dial Knob (n: program number).
  • Page 24: Display During Program Operation

    4.4.2. Display during Program Operation During program mode operation, the instrument displays its operating status, ‘’ program number and current step’’  ‘’remaining time of current step’’  ’’ the current number of repeats in infinite loop”or“the current of iterations and total number of iterations” “process value and set value for RPM process value and set value for temperature”.
  • Page 25: Editing User Program

    WIGGENS Heating Shaker WS-350B [Program 2 and step 3]  [ramping to step 3 temperature]  [1st iteration of 5 total repeats]  [RPM-PV : 500, SV : 500]  [temperature-PV : 23.5℃, SV : 60℃] 4.4.3. Editing User Program Press PROGRAM when the instrument is on standby.
  • Page 26 [In case of setting 3 steps for program 2] The program is reset and generates new steps as many as step SV. Then you can set SVs for temperature, (5) time and shaking RPM for each step by using Dial Knob. Push dial Enter the target temperature and time for the step, and then save the entered value (6)...
  • Page 27 WIGGENS Heating Shaker WS-350B [Enter time for the step] [Enter shaking RPM for the step] [Save the entered value ] If you skip the saving process, all entered inputs are not saved. By applying (5) and (6) for every step, you can complete program editing.
  • Page 28: Viewing Program Setting

    After saving all generated steps, push START/STOP to escape program editing. (7) Notice: If you want to write a new program on a program slot, set step size of the program  slot. The step size setting includes clearing existing program and initializes a new ...
  • Page 29: Stopping Program

    WIGGENS Heating Shaker WS-350B Turn Dial Knob to find a specific step you want to view. Push Dial Knob to get into the step. (4) Turn dial Push dial [In case of viewing step No. 3] And then you can view the SVs of temperature, time and RPM for the step..
  • Page 30: Confirming Program End

    4.4.6. Confirming Program End “END PR n” is displayed with sound alarm at the end of a program (n: program number). Please check the program end by pressing START/STOP. 4.4.7. Resetting User Program 4.4.7.1. Reset All User Programs Press PROGRAM at standby. (1)...
  • Page 31 WIGGENS Heating Shaker WS-350B 4.4.7.2. Reset Individual User Program Press PROGRAM at standby. (1) (2) Select a target program that needs to be deleted. [In case of resetting program 2] (3) Turn Dial Knob to the right and push “PR n EDIT” (n: program number).
  • Page 32: Interval Mix Program

    4.4.8. Interval Mix Program The WS-350B provides 3 standard Interval Mixes that help enzyme reaction be more efficient. Interval Mixes are programmed in advance as follows. Interval mix program SV (Set Value) INTV INTV INTV T 45 T 55 T 65...
  • Page 33: How To Replace A Block

    WIGGENS Heating Shaker WS-350B Turn right Push dial [In case of repeat 5 times] Turn left Push dial [In case of repeat infinitely] Notice: Operating Interval Mix Program is almost the same to operating User Program. 4.5. How to Replace a Block Take out of the plug.
  • Page 34: Offset

    [Cover Seperation] [Block Seperation] Place another block and cover in order. Fix PEEK wrench bolt to the cover by turning hexagon wrench (4) clockwise. Caution: Before replacing a block, please detach tubes, vials, microplate etc. from the block.  You should be careful in handling the instrument. The instrument and its ...
  • Page 35: Safety Device

    WIGGENS Heating Shaker WS-350B Select the offset value by turning the Dial Knob appropriately and, when selected, save it by pushing Dial (3) Knob. When properly saved, the save confirmation screen will appear as shown below: [Offset value selection 0.5℃] After confirm saving offset, setting a SV temperature is automatically displayed as below.
  • Page 36: Maintenance

    activate this device to protect the instrument. Notice: The instrument should be used after fully cooling it down when the instrument is switched off by these kinds of the protection devices. 6. Maintenance 6.1. Periodic Maintenance Classifications A period of checking time daily weekly Power cord...
  • Page 37: Accessories

    WIGGENS Heating Shaker WS-350B 6.2.2. Accessories Remove a contaminant by cleaning the instrument frequently with a soft cloth before and after using, otherwise it cannot be readily wiped out for a long time Keep the instrument cleans always without any contaminant.
  • Page 38: Trouble During Operation

    instrument is together, if so you should not use overly. turned on or Product internal circuit problem Request service. running. Room circuit Too many plugs connect at 1. Check the circuit breaker size along with the breaker trips often the same time. voltage and current supplied to it.
  • Page 39 WIGGENS Heating Shaker WS-350B 8. Blocks Order No. Block dimension (W x D x H, mm) 0.5 ml tube block Block W5012451 98 x 76.5 x 30 1.5 ml tube block Block W5012452 98 x 76.5 x 41 50 ml tube block...
  • Page 40: Appendix

    9. Appendix 9.1. Technical Specification Heating shaker WS-350B Order No. W5012350 Control range (℃) Ambient + 5 to 100 Display up to 100℃, 0.1℃ resolution Fluctuation at 80℃ Temp. Variation at 80℃ Heater output, max. (W) Control Feedback control PID...
  • Page 41: General

    ▪ Defect and trouble ▪ Application and using condition. 9.3. Disclaimer Wiggens does not cover the following cases even it is within the warranty period. ▪ Abuse, ▪ Misuse, neglect, and accident ▪ Improper application, repair or attempt repair not authorized by Wiggens.
  • Page 42: Contact /Technical Service

     Copy and complete the Conformation of condition of unit from these operating instructions.  Please repack the device appropriately for transport and send to WIGGENS Instruments together with the Confirmation of condition of unit. Our contact details WIGGENS GmbH Add:Gässlesweg 22-24, 75334 Straubenhardt, Germany...
  • Page 43: Confirmation Of Condition Of Unit

    WIGGENS Heating Shaker WS-350B Confirmation of condition of unit In the case of repair, copy and complete the Conformation of condition of unit and send it to WIGGENS Instruments. 1. Details about the unit Product number Serial number Reason for repair 2.
  • Page 44 Phone E-mail...
  • Page 45 WIGGENS Heating Shaker WS-350B WIGGENS GmbH Gässlesweg 22-24, 75334 Straubenhardt, Germany Tel.: 0049 7248 4529088 WIGGENS China Room 303, Hall C, Office Building M8, No.1 Jiuxianqiao East Road, Chaoyang District, Beijing 100015, China Tel: +86 400-809-2068 Fax: +86 400-809-2068-112 info@ wiggens.com www.wiggens.com...

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