Sign In
Upload
Download
Table of Contents
Contents
Add to my manuals
Delete from my manuals
Share
URL of this page:
HTML Link:
Bookmark this page
Add
Manual will be automatically added to "My Manuals"
Print this page
×
Bookmark added
×
Added to my manuals
Manuals
Brands
Thermo Scientific Manuals
Laboratory Equipment
Evolution 220
User manual
Thermo Scientific Evolution 220 User Manual
Uv-vis instruments
Hide thumbs
1
2
Table Of Contents
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
page
of
500
Go
/
500
Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
Table of Contents
About the Software and Hardware
Introduction
Overview
About this Document
Before Using the Spectrophotometer
Setting up the Instrument
Contacting Us
E-Mailing Data Files
Product Warranty
Items Not Covered by Warranty
Warranty Period
Warranty Repair
Shipping Cost for Items Covered by Warranty
Trademarks
Update Policy
Site Preparation and Safety
Applications
Fixed
INSIGHT Software
Quant
Rate
Scan
The INSIGHT Window
Action Buttons
Navigation Pane
Other Navigation Pane Features
Task Buttons
Right Pane
Data Display
Menus
Instrument Status Monitors
Measuring Samples
Operation
Thermo Software IQ
Performing a Quantitative Analysis
Quantifying Sample Data
Performing a Kinetics Measurement
Performing Kinetics Measurements
Using the Palette Tools and View Finder
Using the Selection Tool
Using the Spectral Cursor Tool
Using the Peak Area Tool
Using the Peak/Valley Measurement Tool
Using the Region-Threshold Tool
Using the View Finder
Setting Application Parameters
Fixed
Scan
Quant
Rate
Sharing Custom Templates
Managing Data
My Data
Reports
Adding Two Scan Spectra
Converting Scan Data to a Derivative
Converting Scan Data to Other Units
Math and Analysis Operations
Normalizing Scan Data
Ratioing Two Scan Spectra
Smoothing Scan Data
Subtracting a Scan Spectrum
Averaging Scan Spectra
Adding, Subtracting, Multiplying or Dividing Scan Data by a Factor
Finding Peaks in Scan Data
Finding Value Level Crossings in Scan Data
Applications Tab
Options
Report Master Page Tab
Data Store Tab
Preferences Tab
Formulas and Units Tab
Alignment Tab
System Settings
Calibrations Tab
Keyboard Shortcuts
Lamp Tab
System Tab
Evolution 200 Series Spectrophotometers
Safety Considerations
Safety Label Locations
Lifting or Moving the Instrument
Operating Precautions
Local Control or Computer Control
Opening and Closing the Sample and Reference Compartment Doors
Spectrophotometer Basics
Connectors
INSIGHT Software
Keypad
Touchscreen
Inside the Sample Compartment
Outside Cover
Removable Panels
Sample Compartment Front Panel
Tubing Access Panel
Mercury Lamp Access Cover
Detector Access Cover
Corrosion Protection
Detector Access Panel
Installing Sample Compartment Windows
Purging the Sample Compartment
10 MM Rectangular Sample Cell Holder
Standard Cell Holders
Rectangular Reference Cell Holder
Installing a Manual Accessory
Installing a Smart Accessory
Installing a Liquid Thermostatted Accessory
Computer Control Instruments
Installing a Reference Cell Holder
Using the Spectrophotometer
Setting up a Computer Control Instrument
Turning off a Computer Control Instrument
Local Control Instruments
Setting up a Local Control Instrument for Touchscreen Operation
Setting up a Local Control Instrument for Remote Operation
Turning off a Local Control Instrument
Keypad Operation
Cleaning the Instrument
Keypad Shortcuts
Maintenance
Routine Maintenance
Cleaning the Touchscreen
Removing and Installing the Detector Module
Ordering Parts
About the Life Science Applications
Life Science Applications
Nucleic Acid
Nucleic Acid Concentration Calculations
Overview
Accessories Tab for Nucleic Acid
Application Settings
Instrument Tab for Nucleic Acid
Measurement Tab for Nucleic Acid
Samples Tab for Nucleic Acid
Type Tab for Nucleic Acid
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
Nucleic Acid Labels
Dye/Chromophore Editor
Overview
Application Settings
Instrument Tab for Nucleic Acid Labels
Measurement Tab for Nucleic Acid Labels
Type Tab for Nucleic Acid Labels
Accessories Tab for Nucleic Acid Labels
Samples Tab for Nucleic Acid Labels
Unique Screen Features
Making Nucleic Acid Labels Measurements
Oligo Calculator
Overview
Protein A280
Accessories Tab for Protein A280
Application Settings
Instrument Tab for Protein A280
Measurement Tab for Protein A280
Samples Tab for Protein A280
Type Tab for Protein A280
Making Protein A280 Measurements
Unique Screen Features
Proteins and Labels
Application Settings
Dye/Chromophore Editor
Instrument Tab for Proteins and Labels
Measurement Tab for Proteins and Labels
Overview
Type Tab for Proteins and Labels
Accessories Tab for Proteins and Labels
Samples Tab for Proteins and Labels
Making Proteins and Labels Measurements
Unique Screen Features
Pierce BCA
BCA Kits and Protocols
Overview
Application Settings
Measurement Tab for Pierce BCA
Type Tab for Pierce BCA
Standards Tab for Pierce BCA
Instrument Tab for Pierce BCA
Accessories Tab for Pierce BCA
Samples Tab for Pierce BCA
Making Pierce BCA Measurements
Working with Standard Curves
Accessories Tab for Protein Bradford
Application Settings
Bradford Reagent Kits and Protocols
Instrument Tab for Protein Bradford
Measurement Tab for Protein Bradford
Overview
Protein Bradford
Samples Tab for Protein Bradford
Standards Tab for Protein Bradford
Type Tab for Protein Bradford
Bradford Standard Curves
Making Protein Bradford Assay Measurements
Pierce Modified Lowry
Accessories Tab for Pierce Modified Lowry
Application Settings
Instrument Tab for Pierce Modified Lowry
Measurement Tab for Pierce Modified Lowry
Modified Lowry Kits and Protocols
Overview
Samples Tab for Pierce Modified Lowry
Type Tab for Pierce Modified Lowry
Lowry Standard Curves
Making Pierce Modified Lowry Assay Measurements
Pierce 660 Nm Protein Assay
Application Settings
Overview
Pierce 660 Nm Reagent and Protocols
Type Tab for Pierce 660 Nm Protein Assay
Measurement Tab for Pierce 660 Nm Protein Assay
Standards Tab for Pierce 660 Nm Protein Assay
Instrument Tab for Pierce 660 Nm Protein Assay
Accessories Tab for Pierce 660 Nm Protein Assay
Samples Tab for Pierce 660 Nm Protein Assay
Pierce 660 Nm Assay Workbooks
Standard Curves
Making Pierce 660 Nm Protein Assay Measurements
Protein Biuret
Accessories Tab for Protein Biuret
Application Settings
Instrument Tab for Protein Biuret
Measurement Tab for Protein Biuret
Overview
Samples Tab for Protein Biuret
Standards Tab for Protein Biuret
Type Tab for Protein Biuret
Making Protein Biuret Assay Measurements
Standard Curves
Accessories
Degree Near Normal Specular Reflectance
15 Degree Specular Reflectance
30 Degree Specular Reflectance
45 Degree Specular Reflectance
60 Degree Specular Reflectance
Calibrated Validation Carousel (CVC)
Installing Complete CVC in the Instrument
Installing the Carousel on the Smart Base Unit
Initialization
Loading CVC Calibration Data
CVC Test Limits
Running Performance Verification Tests
Using the CVC
CVC Test Results
Viewing CVC Test Reports
Maintenance
Routine Maintenance
Recertification
Depolarizer
Grazing Angle Specular Reflectance
Integrated Fiber Optic Module
Installation
Attaching Cables and Probes
Maintenance
Routine Maintenance
Aligning the Accessory
Troubleshooting
Integrating Sphere Diffuse Reflectance
Mercury Lamp
Installation and Operation
Operating Precautions
Getting Started
Nanocell
Overview
Introduction to Small Volume Measurements
Installing the Nanocell
Loading a Sample
Using the Nanocell
Measurements
Cleaning the Nanocell
Storing the Nanocell
Concentration
Direct UV Assays for DNA, RNA, Oligonucleotides and Protein Concentration
Pathlength Correction Factors
Frequently Asked Questions
TPS-1500W Sealed Peltier Recirculator
General Safety Information
Front and Back Panel
Product Description
Specifications
Preinstallation
Verifying the Power Supply and Fuse
Changing the Voltage Setting or Fuse
Installation
Operation
Cleaning the Accessory
Maintenance
Routine Maintenance
Filling the Reservoir
Acoustic Alarm
Other System Failures
Water Level Failure
Peltier Thermostatted Single Cell Holder
General Safety Information
Product Description
Temperature Controller Front and Rear Panels
Specifications
Preinstallation
Changing the Voltage Setting or Fuse
Verifying the Power Supply and Fuse
Installation
Operation
Setting the Target Temperature and Stir Speed
Using INSIGHT Software to Set Temperature and Stir Speed
Using the Peltier Controller to Set Temperature and Stir Speed
Cleaning the Accessory
Routine Maintenance
Troubleshooting
Probe Handling and Maintenance
Installation
Operation
Maintenance
Routine Maintenance
Rapid MIX Accessory
Cylindrical Reference Cell Holder
Rectangular Reference Cell Holder
Reference Cell Holders
Sample Position Cell Holders
Water Thermostatted 10 MM Rectangular Cell Holder
Cell Holder for 15 MM Beam Height Cells
Cylindrical Cell Holder
Solid Sample Holders
Rotatable Test Tube and Cell Holder
Universal Sample Holder Slide
Sample Holder for 1-Inch Square Cells
Sample Holder for Small Test Tubes and Vials
Variable Pathlength Rectangular Cell Holder
Variable Pathlength Cell Holder Slide
Installation
Installing a Smart Cell Changer
Operation
Replacing the Cell Holder
Smart Linear 8-Cell Changer
Calibrating the Cell Changer
Cell Changer Settings
Displaying the Cell Changer Status Monitor
Cleaning the Accessory
Routine Maintenance
Troubleshooting
SPE-8W Smart Peltier Linear Cell Changer
General Safety Information
Product Description
Temperature Controller Front and Rear Panel
Changing the Voltage Setting or Fuse
Preinstallation
Verifying the Power Supply and Fuse
Installation
Calibrating the Cell Changer
Initializing the Cell Changer
Operation
Configuring the Cell Changer
Setting the Target Temperature and Stir Speed
Measuring Samples with the Cell Changer
Cleaning the Accessory
Maintenance
Routine Maintenance
Troubleshooting
Smart Rotary Cell Changer
Installation
Installing a Smart Cell Changer
Operating Precautions
Replacing the Cell Holder
Safety Considerations
Cell Changer Settings
Displaying the Cell Changer Status Monitor
Initializing the Cell Changer
Measuring Samples with a Cell Changer
Measuring Standards with a Cell Changer
Operation
Cleaning the Accessory
Maintenance
Routine Maintenance
Safety Considerations
Smart Sipper
Installation
Installing a Flow Cell
Installing Pump Tubing
Installing the Smart Sipper
Operating Precautions
Calibration
Operation
Cleaning Flow Cells
Cleaning the Accessory
Maintenance
Replacing Flow Cell Tubing
Replacing Pump Tubing
Routine Maintenance
Pump Tubing Chemical Compatibility
Specifications
Troubleshooting
Operating Precautions
Temperature Probe
Installation
Operation
Configuring a Target Temperature
Displaying a Temperature Status Monitor
Temperature Settings
Cleaning Temperature Probes
Maintenance
Replacing Stopper O-Rings
Routine Maintenance
Troubleshooting
Variable Angle Transmission
Fiber Optic Probes
Operating Precautions
VERSA Probe
Fiber Optic Cables
Installation
Installing the VERSA Probe
Installing Tips
Operation
Routine Maintenance
Advertisement
Quick Links
1
Table of Contents
2
About the Software and Hardware
3
Setting up the Instrument
Download this manual
UV-Vis Instruments
Evolution 200 Series
User Guide
269-251400 Revision A
August 2010
Table of
Contents
Previous
Page
Next
Page
1
2
3
4
5
Advertisement
Table of Contents
Troubleshooting
Troubleshooting
284
Troubleshooting
343
Troubleshooting
379
Troubleshooting
408
Troubleshooting
442
Troubleshooting
454
Need help?
Do you have a question about the Evolution 220 and is the answer not in the manual?
Ask a question
Questions and answers
Related Manuals for Thermo Scientific Evolution 220
Laboratory Equipment Thermo Scientific Matrix 1029 User Manual
Electronic pipette (66 pages)
Laboratory Equipment Thermo Scientific Thermo Scientific Sorvall Legend Micro Series Instruction Manual
(94 pages)
Laboratory Equipment Thermo Scientific Orion 2111XP Manual
Sodium analyzer (149 pages)
Laboratory Equipment Thermo Scientific Precision 280 Installation And Operation Manual
Microprocessor-controlled general purpose water baths (26 pages)
Laboratory Equipment Thermo Scientific Heraeus Fresco 21 Instructions For Use Manual
(50 pages)
Laboratory Equipment Thermo Scientific Heracell Vios 160i CR Operating Instructions Manual
(216 pages)
Laboratory Equipment Thermo Scientific Heracell VIOS 250i Supplement To Operating Instructions
Co2 incubators (20 pages)
Laboratory Equipment Thermo Scientific VARIOKLAV 25T Operating Manual
Steam sterilizer (60 pages)
Laboratory Equipment Thermo Scientific 2870 Operating And Maintenance Manual
Reciprocal shaking water baths (62 pages)
Laboratory Equipment Thermo Scientific Jewett 430 Installation & Operation Manual
-30 c laboratory freezers v and w (30 pages)
Laboratory Equipment Thermo Scientific 180 Series Operating And Maintenance Manual
Precision general purpose water baths (36 pages)
Laboratory Equipment Thermo Scientific 2860 Service Manual
Coliform incubator bath (23 pages)
Laboratory Equipment Thermo Scientific Shandon TissueWave 2 Operator's Manual
Microwave specimen processing system (62 pages)
Laboratory Equipment Thermo Scientific Heracell 150i GP Operating Instructions Manual
(193 pages)
Laboratory Equipment Thermo Scientific Thermo Scientific Barnstead Nanopure Life Science 2114 Series Operating Manual And Parts List
Uv/uf ultrapure water system (74 pages)
Laboratory Equipment Thermo Scientific HERACELL VIOS 160i LK User Manual
Co2 incubator (234 pages)
Table of Contents
Save PDF
Print
Rename the bookmark
Delete bookmark?
Delete from my manuals?
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL
Need help?
Do you have a question about the Evolution 220 and is the answer not in the manual?
Questions and answers