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Cole Parmer SP-300-VIS Instruction Manual

Spectrophotometer
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Cole-Parmer
®
SP-300-VIS
SP-300-UV
Spectrophotometer
Instruction Manual
730 005-CPB Rev K

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  • Page 1 Cole-Parmer ® SP-300-VIS SP-300-UV Spectrophotometer Instruction Manual 730 005-CPB Rev K...

  • Page 2: Safety

    Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed to be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.
  • Page 3 9. ATTENTION. Si l’appareil n’est pas utilisé de manière adéquate, la protection de l’appareil pourrait être impactée. 10. Ne pas remplacer le cordon d’alimentation fourni par un cordon d’alimentation de dimension électrique non adapté. Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch. 1.
  • Page 4 Questo strumento utilizza una sorgente di luce UV. Non guardare direttamente la sorgente di luce. AVVERTENZA: Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile. 9. AVVERTENZA: qualora il dispositivo non venga utilizzato nel modo descritto, la protezione fornita dal dispositivo stesso potrebbe risultare compromessa.

  • Page 5: Table Of Contents

    Contents Safety..............................2 Contents ..............................5 SECTION 1 - Introduction ........................7 INSTRUMENT DESCRIPTION .....................7 IMPORTANT SAFETY ADVICE ....................7 INSTRUMENT SPECIFICATION ..................8 SECTION 2 – Installation ........................8 UNPACKING .........................8 INSTALLATION ........................9 DISPLAY..........................10 CONTROLS ........................10 REAR PANEL........................11 FRONT PANEL ........................11 SECTION 3 – Theory and Practice of Spectroscopy Measurements..........12 THEORY OF SPECTROSCOPY MEASUREMENT ............12 SPECTROSCOPY MEASUREMENT .................13 GOOD PRACTICE GUIDELINES ..................14...
  • Page 6 6.5.2 Changing the Concentration Factor................28 6.5.3 Changing the Calibration Standard Value ..............28 SECTION 7 – PRINTING AND AUTOLOGGING..................29 PRINTING ...........................29 7.1.1 Print Setup ........................29 7.1.2 Printing Results......................30 AUTOLOGGING .........................30 7.2.1 Setting the Number of Sample Repetitions..............30 7.2.2 Selecting Result’s Destination ..................31 CONNECTING TO A PC .....................32 SECTION 8 –...

  • Page 7: Section 1 - Introduction

    INSTRUMENT DESCRIPTION The SP-300-VIS and SP-300-UV spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. The SP-300-VIS is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The SP-300-UV is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm.

  • Page 8: Instrument Specification

    Weight SECTION 2 – Installation UNPACKING Remove the SP-300-VIS or SP-300-UV from the packaging and ensure the following items are included: 1. Model SP-300-VIS spectrophotometer, or Model SP-300-UV spectrophotometer 2. 24V 65W power supply unit (021 060) 3. SP-300-VIS - Pack of 100 disposable plastic visible wavelength cuvettes (060 084), SP-300-UV - Pack of 100 disposable UV plastic cuvettes (060 230) 4.

  • Page 9: Installation

    INSTALLATION Models SP-300-VIS and SP-300-UV are supplied ready to use. The unit should be placed on a clean flat surface which is free from drafts and vibrations. The units are designed for operation on 90V to 264V AC input at 47 to 63Hz. Select the correct plug attachment and attach to the power supply unit as shown below: Fig 2.2.1 –...

  • Page 10: Display

    DISPLAY These spectrophotometers have a dot matrix display which enables icons to be displayed clearly. Following successful completion of the power on tests the main menu screen will be displayed: Fig. 2.3 – Display CONTROLS The keypad used for these models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results.

  • Page 11: Rear Panel

    REAR PANEL The image below shows the rear panel on the instrument: Fig. 2.5.1 – Rear Panel 1. Lamp access panel Allows access to lamp when replacement is necessary 2. Power switch On/off switch for the unit 3. Power in socket Connection socket for power supply unit 4.

  • Page 12: Section 3 - Theory And Practice Of Spectroscopy Measurements

    SECTION 3 – Theory and Practice of Spectroscopy Measurements THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample.

  • Page 13: Spectroscopy Measurement

    The optical layout of the SP-300-VIS and SP-300-UV spectrophotometers is shown below: Figure 3.2.1 – Diagram of light path...

  • Page 14: Good Practice Guidelines

    GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme.
  • Page 15 5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible. If stored in stoppered and sealed cuvettes, they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material.

  • Page 16: Section 4 - Instrument Setup

    SECTION 4 – Instrument Setup NAVIGATING AND SCREEN SETUP The main menu screen is displayed below. Fig 4.1 – Home Screen To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen. In the main menu either of the two soft keys adjacent to the measurement mode icon can be pressed to access the mode.

  • Page 17: Time And Date

    TIME AND DATE The time and date menu enables the current time and date to be set. This information will be saved on all results and displayed on printouts. The time and date menu can be accessed from the main menu by holding the key below the time and date icon for 2 seconds.

  • Page 18: Screen Contrast

    LAMP SAVE This function is only available on the SP-300-VIS visible spectrophotometer which uses a tungsten halogen lamp. The lamp save function enables the time in...
  • Page 19 The time set will begin to count down when there is no lamp activity. When the count down is complete the lamp and the fan will be turned off and the lamp save icon is shown in all the measurement modes. To bring the instrument out of the lamp save in order to perform a measurement press the key below the lamp save icon.

  • Page 20: Section 5 - Photometrics

    SECTION 5 – PHOTOMETRICS The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at one wavelength and at one point in time. There are no post measurement calculations available in this measurement mode. MODE SPECIFIC PARAMETERS The photometrics operating menu enables measurement parameters to be changed.

  • Page 21: Calibration

    CALIBRATION The calibration must be performed at the same wavelength at which the sample will be measured. Insert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key below the calibrate to zero absorbance icon.

  • Page 22: Section 6 - Concentration

    SECTION 6 – CONCENTRATION The concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time.

  • Page 23: Settings

    This will open a number entry screen. Use the keys at the bottom of the screen to select the digit to be adjusted. Use the keys adjacent to the arrow icons to increase or decrease the wavelength to the required number. Press the key adjacent to the tick icon to save the changes and return to the settings menu.

  • Page 24: Changing The Resolution

    save and return to the settings menu. The selected unit will be displayed in the minimal and operating menu along with absorbance and selected wavelength. 6.2.2.2 Changing the Resolution The resolution that the concentration is displayed as can be selected from 1, 0.1, 0.01 or 0.001 by repeat presses of the key below the resolution icon in the settings menu.

  • Page 25: Calibration

    If the factor is not known a standard should be measured in order to calculate the factor. If a standard is used the factor value should be set to 1.000. CALIBRATION concentration measurement mode calibrations against a standard or a factor can be performed following a zero calibration.

  • Page 26: Calibrating To A Factor

    6.3.2 Calibrating to a Factor Insert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key below the calibrate to zero absorbance icon. The instrument will calibrate to zero absorbance. Once the calibration is complete the sample can be measured using the measure to factor icon.

  • Page 27: Changing Concentration Units

    6.5.1 Changing Concentration Units Users can adjust the displayed concentration unit after a measurement. If the measurements concentration unit is updated, the instrument will adjust the displayed concentration value to reflect the new selection. This functionality is limited to ensure that the user can only update the measurement unit to one that is a direct conversion from the original measurement unit.

  • Page 28: Changing The Concentration Factor

    The updated concentration value and unit are shown in the main display. 6.5.2 Changing the Concentration Factor Users cannot adjust the concentration factor once a blank measurement is performed. If the concentration factor needs to be changed, the user must exit and re-enter the concentration measurement mode.

  • Page 29: Section 7 - Printing And Autologging

    SECTION 7 – PRINTING AND AUTOLOGGING The utility toolbar in the operating menu provides access to printing, print setup options, and autologging options. Print/print settings Autolog menu Fig 7.1 - Operating Menu PRINTING The utility toolbar in the operating menu enables results to be printed and print setup options to be set.

  • Page 30: Printing Results

    printer. The results can only be sent to the internal printer if there is an internal printer connected. To select the internal printer for the printout destination press the key adjacent to the printer icon. Once the required printout destination and language has been selected press the key adjacent to the tick icon to save and return to the operating menu.

  • Page 31: Selecting Result's Destination

    To set the time period between each measurement press the key below the timer icon and use the keys adjacent to the arrow icons to increase or decrease the time in 1 second intervals. To reset the time to one second press and hold the key below the timer icon for 2 seconds.

  • Page 32: Connecting To A Pc

    CONNECTING TO A PC Connect the interface cable to the RS232 serial port on the rear of the instrument and connect to the serial port on the rear of the PC. Turn the PC on and load the PC software by inserting the PC software disc into the CD drive.

  • Page 33: Section 8 - Accessories And Spare Parts

    SECTION 8 – Accessories and Spare Parts OPTIONAL ACCESSORIES Part Code Description of Accessory 660 101 Internal printer 735 401 Automatic 8 cell turret 735 201 Sipper pump 735 301 Peltier 735 701 Combined sipper Peltier pump 630 204 10x10mm path length cuvette holder 637 071 16/24mm test tube holder 630 005...

  • Page 34: Passive Accessories

    Insert the paper roll into the printer – ensuring that there is some paper sticking out of the printer before clicking the grey plastic back into place. Switch the instrument on. The power and error lights on the printer will flash. Once the instrument power on tests are complete press the feed button to check that the paper is fed correctly.
  • Page 35 For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in the forwards direction. Once the base plate and front panel have been removed insert the water-heated cuvette holder into the chamber, ensuring the base plate rests flat in the unit.
  • Page 36 Fit the custom front panel to the unit ensuring the metal pipes on the accessory are aligned and fed through the two holes in the front panel. The metal pipes should protrude from the outer casing. Secure the custom accessory front plate to the unit with screws 5 and 6.

  • Page 37: Active Accessories

    8.2.3 Active Accessories Unscrew the thumb screw to undo the passive accessory. Lift out the passive accessory. To fit an active accessory unscrew screws 1 to 4 and lift out the metal base plate. This will expose the bottom of the sample chamber with the power supply connection needed to operate the active accessories.

  • Page 38: Peltier

    8.2.3.2 Peltier For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in the forwards direction. Take the Peltier base plate.

  • Page 39: Sipper Pump

    8.2.3.3 Sipper pump For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in the forwards direction. Take the sipper base plate.
  • Page 40 For sipping: 1. Connect the sipper pump tubing to the outlet port on the flow-through cuvette. 2. Secure the tubing using the clip on the righthand side of the pump head. 3. Ease the tubing round the rollers by carefully rotating them clockwise, by hand.

  • Page 41: Combined Sipper Peltier Pump

    5. Fit the other end of the tubing onto the outlet port, located on the inside of the front bulkhead. 6. Connect a suitable length of sipper pump tubing to the external outlet port. 7. Insert one end of the capillary tube into the sipper pump tubing, as shown. 8.

  • Page 42: Using The Accessories

    8.3 USING THE ACCESSORIES 8.3.1 Automatic 8 cell turret When the automatic 8 cell turret is in use the 8 cell turret icon is displayed in the bottom right hand corner of the screen. The current cell position is displayed adjacent to the 8 cell turret icon. The 0 position should always be used for the zero calibration blank sample.

  • Page 43: Sipper Pump

    This opens the Peltier settings screen. Use the keys at the bottom of the screen to select the digit to be changed and use the arrow icons to increase or decrease the number. The temperature can be set in °C or °F by pressing the key adjacent to the °C icon.

  • Page 44: Manual Sipper Pump Settings

    8.3.3.1 Manual Sipper Pump Settings To operate the sipper pump in manual mode press the key adjacent to the manual sipper icon. Select the preferred pump direction by pressing the key below the forwards or backwards arrow. Press the key adjacent to the tick icon to save and return to the operating menu.

  • Page 45: Timed Sipper Pump Settings

    8.3.3.2 Timed Sipper Pump Settings To operate the sipper pump in timed mode press the key adjacent to the timed sipper pump icon. Press the key below the calibrate timed sipper icon. Select the required pump direction by pressing the key below the forwards or backwards arrow.
  • Page 46 This stage allows an air gap to be added to the calibration sequence. If an air gap is not required press the key below the 001 icon to set the air gap to one. If a previously programmed air gap is to be used press the key adjacent to the double greater than icon to skip this stage and retain the current air gap time.
  • Page 47 To perform a measurement place the sipper tubing into the sample and press the key below the sipper pump icon. Confirmation will be needed to start the sipper pump. Press the key adjacent to the cross icon to cancel and return to the operating menu. Press the key adjacent to the tick icon to confirm and start the sipper pump.

  • Page 48: Combined Sipper Peltier Pump

    8.3.4 Combined sipper Peltier pump When the combined sipper Peltier is in use the sipper Peltier icon is displayed in the bottom right hand corner of the screen. The current temperature is displayed above the set point temperature adjacent to the sipper Peltier icon. Adjacent to the current temperature is an arrow to indicate if it is below or above the set temperature.

  • Page 49: Section 9 - Maintenance And Service

    9.2.1 Tungsten Halogen Lamp Replacement This option is only valid for SP-300-VIS spectrophotometers. Before replacing the lamp disconnect the unit from the mains supply and ensure the lamp is cool before handling. Access to the tungsten halogen lamp can be gained via the lamp access panel located on the rear of the instrument (refer to section 2.5).

  • Page 50: Xenon Lamp Module Replacement

    Cole-Parmer Ltd UV but excluded the Tungsten lamp used in the SP-300-VIS which is covered by a 1 year warranty. In the t h e f a u l t . P l e a s e c l e a r l y m a r k t h e p a c k a g e f o r t h e a t t e n t i o n o f t h e S e r v i c e D e p a r t m e n t a n d p o s t event of a justified claim Cole-Parmer will replace any defective component or replace the unit free of charge.

  • Page 51: Section 10 - Troubleshooting

    SECTION 10 – Troubleshooting 10.1 ERROR CODES If an error code is displayed it will be accompanied by a spanner icon and a symbol to indicate if the error is a warning (caution icon) or fatal (stop icon). If the error is fatal contact your local distributor or Cole-Parmer service department (refer to section 9.3).
  • Page 52 The most likely causes of this error are: 1. Lamp failure. 2. Deteriorating lamp signal. 3. Sample or cuvette in the sample holder. Solution: Fatal Ensure the sample holder is empty. Restart the unit, if the problem persists contact a service technician. Err 8 Unable to find Vane on Turret This error indicates that the vane position zero on the turret...

  • Page 53: Troubleshooting Guide

    10.2 TROUBLESHOOTING GUIDE Issue Solution Unable to achieve zero absorbance or Ensure that there is not a sample in the sample 100% transmittance when calibrating chamber. Ensure the instrument lid is closed before and during the calibration. Ensure the lamp is working – if the lamp has failed replace the lamp (7300) or lamp module (SP-300-UV).

  • Page 54: Section 11 - Declaration Of Conformity

    This product meets the applicable CE Directives that interference will not occur in practise. Where and UKCA Legislation for radio frequency there is a possibility that injury, damage or loss might interference and may be expected not to occur if equipment malfunctions due to radio interfere with, or be affected by, other equipment with similar frequency interference, or for general advise before qualifications.

  • Page 55: Section 12 - Glossary Of Icons

    SECTION 12 – Glossary of Icons Mode ICON Description Common Back key Common Tick icon - Done/yes Common Cross icon – Cancel/no Common Printer icon - Print/open printer settings Common No results to send to printer Common Computer icon - RS232 serial port for connection to an external printer or a computer Common No results to send to RS232...
  • Page 56 Main Menu Opens photometrics measurement mode Main Menu Instrument settings Main Menu 12.00 Time/date icon Time & Date Clock icon - Set time Time & Date Calendar icon - Set date Time & Date Toggle icon – Switches date format Autolog Autolog icon - Opens auto log menu Autolog...
  • Page 57 Concentration Factor menu Concentration Standard menu Concentration Resets standard or factor value to one Concentration Check standard Accessories No single cell - Method created on a unit with a single cell accessory fitted Accessories 8 cell turret icon – the automatic 8 cell turret is in use Accessories Error finding cell position 0 on turret Accessories...
  • Page 58 Accessories Reduce sample uptake/reduce air gap Accessories Increase sample uptake/increase air gap Accessories No sipper - Method created on a unit with a sipper accessory fitted Accessories Sipper Peltier pump in use Accessories No sipper Peltier - Method created on a unit with a sipper Peltier accessory fitted Accessories PC comms - shown when the instrument is connected to a...

  • Page 60: Ordering Information

    Ordering Information Order Cole-Parmer Cole-Parmer Jenway Jenway Series Model Model Part No. 7300 Visible 83058-15 SP-300 SP-300-VIS Spectrophotometer 730001 90 to 264 V ac 7305 UV/VIS 83058-16 SP-300 SP-300-UV Spectrophotometer 730501 90 to 264 V ac Warranty Registration India T: +44 (0) 1480 272279 T: +9122 61394444 E: uk.sales@antylia.com...

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