Related Manuals for Cole Parmer SP-200-UV
Summary of Contents for Cole Parmer SP-200-UV
- Page 1 Cole-Parmer ® SP-200-UV UV / Visible Spectrophotometer Instruction Manual 635 015-CPB REV J...
- Page 2 Ces informations sont importantes ; veuillez les lire avec attention avant d'installer ou d'utiliser l'instrument. 1. Les spectrophotomètres de la série SP-200-UV sont conçus pour être utilisés par du personnel formé qui connaît les principes et les applications concernés. Pour obtenir de l'aide et des conseils supplémentaires, merci de consulter le site Internet...
- Page 3 Dies sind wichtige Informationen, bitte lesen Sie sie vor Installation oder Verwendung dieses Geräts sorgfältig durch. 1. Die Spektrophotometer der SP-200-UV-Serie sind für den Betrieb durch geschultes Personal, das über die entsprechenden Prinzipien und Anwendungen Bescheid weiß, vorgesehen. Weitere Hilfe und Hinweise finden Sie auf www.Cole-Parmer.de...
- Page 4 Información importante que debe leer atentamente antes de instalar o utilizar este instrumento. 1. Los espectrómetros de la serie SP-200-UV se han concebido para ser utilizados por personal con formación que conozca los principios y las aplicaciones que entrañan. Si necesita más ayuda o indicaciones, diríjase a...
- Page 5 Queste informazioni sono importanti; si prega di leggerle attentamente prima di installare o utilizzare lo strumento. 1. Gli spettrofotometri della serie SP-200-UV sono progettati per essere utilizzati da personale qualificato che conosce i principi e le applicazioni ad essi collegati.
- Page 6 eseguire misurazioni e analisi con questo strumento. Attrezzature idonee per la protezione personale e la sicurezza vanno utilizzate in ogni circostanza. 9. Se si sospetta che la protezione della sicurezza sia stata compromessa in qualsiasi modo, disattivare e rendere inutilizzabile lo spettrofotometro. La condizione di guasto deve essere comunicata al responsabile della manutenzione appropriato.
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Page 7: Table Of Contents
Contents SECTION 1 - Introduction ..................8 INSTRUMENT DESCRIPTION..................... 8 ENVIRONMENTAL CONDITIONS..................8 INSTRUMENT SPECIFICATION..................8 SECTION 2 – Installation ...................9 UNPACKING..........................9 INSTALLATION ........................9 DISPLAY ..........................10 CONTROLS ........................10 REAR PANEL ........................11 SAMPLING ......................... 12 SECTION 3 – Theory and Practice of Spectroscopy Measurements ....... 13 THEORY OF SPECTROSCOPY MEASUREMENT ............ -
Page 8: Section 1 - Introduction
SECTION 1 - Introduction INSTRUMENT DESCRIPTION The SP-200-UV spectrophotometer is suited to a wide range of applications in education, quality control, environmental and clinical analysis. This model is a UV/visible spectrophotometer covering a wavelength range from 198nm to 1000nm, with measurement modes for absorbance, % transmittance and concentration. -
Page 9: Section 2 - Installation
SECTION 2 – Installation UNPACKING Remove the SP-200-UV from the packaging and ensure the following items are included: 1. Model SP-200-UV spectrophotometer 2. Required power cable either UK mains lead moulded plug (HH179(S)), EU mains lead moulded plug (HH180(S)), or US mains lead moulded plug (FCABLEUS) 3. -
Page 10: Display
display before displaying the main measurement screen. Please note that the instrument will go to the last wavelength and measurement mode used. DISPLAY This spectrophotometer has an LCD display: Fig. 2.3.1 – Display 1. Primary display area – Transmission, Absorbance, Concentration 2. -
Page 11: Rear Panel
Fig. 2.4.1 – Controls 1. Arrow Keys: Used to adjust values on the selected display 2. Arrow Keys: Used to move the cursor horizontally between menu options 3. Return Key: Used to select the display menu option 4. CAL Key: Initiates a calibration routine 5. -
Page 12: Sampling
3. Power in socket IEC type connection socket for mains cable 4. Output sockets Analogue output 5. RS232 serial port Connection to a PC SAMPLING Model SP-200-UV is supplied with a 10mm cuvette holder. Other accessories can be fitted (refer to section 7). -
Page 13: Section 3 - Theory And Practice Of Spectroscopy Measurements
SECTION 3 – Theory and Practice of Spectroscopy Measurements THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. -
Page 14: Spectroscopy Measurement
The optical layout of the SP-200-UV spectrophotometer is shown below: Figure 3.2.1 – Diagram of light path The light from the xenon lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths. -
Page 15: Good Practice Guidelines
GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme. - Page 16 h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used. 5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible.
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Page 17: Section 4 - Photometrics Measurements
SECTION 4 – PHOTOMETRICS MEASUREMENTS The SP-200-UV can perform simple measurements of % transmittance or absorbance. The sample is measured at one wavelength and at one point in time. TRANSMISSION MEASUREMENT MODE To select % Transmittance use the LEFT or RIGHT arrow keys to move the cursor to the %T indicator on the menu bar (refer to figure 2.3.1). -
Page 18: Section 5 - Concentration Measurements
SECTION 5 – CONCENTRATION MEASUREMENTS The concentration measurement mode enables simple measurements of concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. -
Page 19: Section 6 - Connecting To A Pc
The sample number is incremented every time the PRINT key is pressed. The following commands can also be sent to the SP-200-UV via the serial interface (using Windows HyperTerminal, for example): ASCII D or d Same as pressing the PRINT key ASCII T<CR>... -
Page 20: Rs232 Output
– LINKED TO RTS DSR 6 – OUTPUT FROM SP-200-UV – OUTPUT FROM SP-200-UV DCD 8 DTR 20 – INPUT TO SP-200-UV (must be active) GND 7 Suggested interconnections are detailed below: SP-200-UV IBM PC XT (25 way “D”) TDX 2... -
Page 21: Recorder Output
SP-200-UV IBM PC XT (9 way “D”) TDX 2 3 TXD (From PC) RXD 3 2 RXD (To PC) RTS 4 7 RTS (From PC) CTS 5 8 CTS (To PC) DSR 6 6 DSR (To PC) DCD 8 1 DCD (To PC) -
Page 22: Section 7 - Accessories And Spare Parts
Interface cable kit 542 009 7.2 SPARES Part Code Description of Spare Part Xenon Flash Lamp Module (SP-200-UV) 012 094 10x10mm path length cuvette holder 630 204 Dual cell holder for 10mm cuvettes and 12.7mm diameter tubes (6320D ONLY) 632 511... -
Page 23: Section 8 - Maintenance And Service
SECTION 8 – Maintenance and Service ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. -
Page 25: Service
SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Cole-Parmer equipment develops a fault. Please contact them by one of the following means with a clear description of the problem: E-mail: cpservice@antylia.com Tel: +44 (0) 1785 810475 Fax: +44 (0) 1785 810471 On occasion it may be necessary for your equipment to be sent back to our service department for repair. -
Page 26: Section 9 - Troubleshooting
Scientific Help (refer to section 9.2). Error Code Error Name Issue This error occurs when the SP-200-UV closes the shutter to Err 1 Dark cal error block light entering the sample chamber. If the detector output does not fall to a level normally associated with a dark cal then this error is indicated. -
Page 27: Section 10 - Declaration Of Conformity
This product meets the applicable CE Directives that interference will not occur in practise. Where and UKCA Legislation for radio frequency there is a possibility that injury, damage or loss might interference and may be expected not to occur if equipment malfunctions due to radio interfere with, or be affected by, other equipment with similar frequency interference, or for general advise before qualifications. -
Page 28: Ordering Information
Ordering Information Order Cole-Parmer Cole-Parmer Jenway Jenway Series Model Model Part No. 6305 UV-Vis 99968-68 SP-200 SP-200-UV 635001 Spectrophotometer 230V 6305 UV-Vis 99968-69 SP-200 SP-200-UV-115 635031 Spectrophotometer 115V Warranty Registration India T: +44 (0) 1480 272279 T: +9122 61394444 E: uk.sales@antylia.com E: info@coleparmer.in...
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