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Related Manuals for Cole Parmer Jenway 7415 NANO
Summary of Contents for Cole Parmer Jenway 7415 NANO
- Page 1 Spectrophotometer 7415 NANO Instruction Manual JEN0003 / Version 1.1...
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Page 2: Table Of Contents
Contents Section 1 - Introduction ....................7 General Description ......................... Important Safety Advice ......................1.3 Symbols Defined ........................Electrical Requirements ......................Section 2 - Installation ....................9 Unpacking ..........................Installation Conditions ......................Overview ..........................10 2.3.1 Main View ........................10 2.3.2 Rear View ......................... - Page 3 Section 7 - Photometrics ....................23 Method Set up ........................23 7.1.1 Selecting a Wavelength ..................... 23 Blank Measurement......................... 24 Sample Measurement ......................24 Section 8 - Concentration .................... 25 Method Set up ........................25 8.1.1 Selecting a Wavelength ..................... 25 8.1.2 Using a Factor ........................
- Page 4 Section 11 - Kinetics ..................... 39 11.1 Method Set up ........................39 11.1.1 Selecting a Wavelength ..................... 39 11.1.2 Setting the Kinetics Measurement Time................40 11.1.3 Setting the Measurement Time Interval ................40 11.1.4 Setting Lag Time ....................... 40 11.1.5 Selecting Absorbance or % Transmittance .................
- Page 5 14.3.2.2 Entering a Factor ......................53 14.4 Blank Measurement......................... 54 14.5 Sample Measurement ......................54 Section 15 - Colorimetric Protein Assays ..............55 15.1 BCA ............................55 15.2 Biuret ............................55 15.3 Bradford ..........................55 15.4 Lowry ............................55 15.5 Pierce 660 ..........................
- Page 6 17.2 Spare Parts ..........................63 Section 18 - Maintenance and Servicing ..............63 18.1 Routine Maintenance ......................63 18.1.1 Cleaning ........................... 63 18.1.2 Read Head Cleaning ......................63 18.1.3 Read Head Re-conditioning ....................64 18.1.4 In Case of Accidental Spillage.................... 64 18.1.5 In Case of Contamination ....................
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Page 7: Section 1 - Introduction
Section 1 - Introduction Thank you for purchasing this Jenway product. To get the best performance from the equipment, and for your own safety, please read these instructions carefully before use. If the equipment is not used in the manner described in this manual and with accessories other than those recommended by the manufacturer, the protection provided may be impaired. -
Page 8: Electrical Requirements
Electrical Requirements THIS INSTRUMENT MUST BE GROUNDED Before connection please ensure that the line supply corresponds to the power requirements below: Power Supply requirements 65 W 100 V - 230 V ~ 50/60 Hz The equipment is provided with a power supply unit and three power cables consisting of a UK 3-pin and a “Schuko” 2-pin plug for 230 V installations and a NEMA 5-15 plug for 120 V installations. -
Page 9: Section 2 - Installation
Section 2 - Installation Unpacking Before discarding the packaging check that all parts are present and correct. 1 7415 Nano 2 Instruction manual 3 UK power lead 4 EU power lead 5 US power lead 6 Power supply unit Installation Conditions When the equipment is used for the first time or moved to a different environmental temperature, it is important to allow the equipment to equalise to the ambient temperature. -
Page 10: Overview
Overview 2.3.1 Main View 2 x USB Type A ports Open and close catch On/Off power switch Colour touchscreen and user interface 2.3.2 Rear View Ethernet (RJ45) port USB Type B port Power inlet socket... -
Page 11: Section 3 - Theory And Practice Of Spectroscopy Measurement
Section 3 - Theory and Practice of Spectroscopy Measurement Theory of Spectroscopy Measurement UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through. The Beer-Lambert law states that: A = Ɛ... -
Page 12: Spectroscopy Measurement
Spectroscopy Measurement There are four main components of a spectrophotometer. These are a light source to emit a high and constant amount of energy over the full wavelength range; a method for separating the light into discreet wavelengths; a sample holder and a light detector. The optical layout of the 7415 Nano spectrophotometer is shown below: Light from Xenon lamp Detected by photodiode detector... -
Page 13: Good Practice Guidelines
Good Practice Guidelines 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required, adherence to Standard Operating Procedures (S.O.P.) and Good Laboratory Practice (G.L.P.) should be monitored with regular calibration checks and a suitable Quality Control (Q.C.) programme. -
Page 14: Section 4 - Instrument Set Up
Section 4 - Instrument Set up Start up Screen The power up screen is shown below: Navigation The main menu is shown below: This spectrophotometer is controlled solely through the touchscreen interface of the equipment and follows a basic Android user interface. If the number of options available in a menu exceeds the number that can be displayed on the screen, swipe to the left to view the other modes. -
Page 15: Methods
Throughout, the software options can be turned ON and OFF using a switch: Option is ON Option is OFF In each measurement mode there is an overflow icon giving additional save and load method options. Touching gives options to load a previously saved method, touch to save the entered method parameters, touch to upload to CPLive* or touch clear recently used method parameters. *Refer to CPLive instructions for more information. When required to enter numbers, a keypad will pop up. -
Page 16: Results
Results Touch to access results that have been saved. Touch the required results to view the details of the result. You will then be able to delete, upload or export the selected result. See Section 16 for more information. Settings Touch to enable access to instrument status, measurement settings, network connections, storage and service settings. -
Page 17: Network Connections
4.5.3 Network Connections Touch to view available network connections. Options include Ethernet (RJ45), IP configuration, Status, IP address and CPLive options. 4.5.4 Storage Touch to view the amount of available storage on the internal memory of the spectrophotometer. If a USB memory stick is inserted the amount of free space on the USB stick will also be shown. -
Page 18: Regional Settings
4.5.5 Regional Settings Touch to view the Time, Date, Time zone and Language options. 4.5.5.1 Setting the Time To set the instrument time touch . Touch and move the clock hand to the correct hour position, repeat the same process for minutes, select AM or PM and touch to apply. -
Page 19: Setting The Date
4.5.5.2 Setting the Date To set the instrument date touch . Scroll up or down to change the month viewed. Touch the required date and touch to apply. To set the year touch and scroll up or down and touch the required date and touch to apply. -
Page 20: Instrument Language
4.5.5.4 Instrument Language The software can be viewed in five different languages with a choice of English, French, German, Italian or Spanish. To select the required language touch and select from the menu. Touch next to the required language to apply. 4.5.6 Service Settings Service Settings are protected from normal use. -
Page 21: Section 5 - Micro Volume Settings
Section 5 - Micro Volume Settings The Micro Volume Settings allows the user to select the required path length (0.2mm or 0.5mm) for a measurement and to calibrate the accessory using a standard solution with known absorbance values at 260 and 330nm. Jenway recommends that users select the required path length before the start of each experiment. -
Page 22: Section 6 - Adding, Removing And Recovering Samples
Section 6 - Adding, Removing and Recovering Samples Adding a Sample The 7415 Nano spectrophotometer is designed to measure sample volumes ranging from 0.5µl to 5.0µl. Jenway recommends that users should, if possible use at least 2.0ul of sample for their measurements. Open the lid of the spectrophotometer and the Read Head Mechanism will open. -
Page 23: Section 7 - Photometrics
Section 7 - Photometrics Jenway recommends that users select the required path length before the start of each experiment. See Section 5 - Micro Volume Settings for more information. The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. -
Page 24: Blank Measurement
Blank Measurement A blank measurement must be performed at the same wavelengths at which the sample will be measured. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. Sample Measurement Once a blank measurement has been performed will become active and a sample can be measured. -
Page 25: Section 8 - Concentration
Section 8 - Concentration The concentration measurement mode enables sample concentrations to be calculated using a standard of a known concentration or a known factor. The sample is measured at one wavelength at one point in time. There are no post measurement calculations available in this measurement mode. Touch the Concentration icon on the main menu to enter this measurement mode. -
Page 26: Using A Standard
8.1.3 Using a Standard If the factor is not known a standard of known concentration can be measured to calculate concentration. Touch to select this option and disable the use factor option. To enter the concentration of the known standard touch the value under standard and use the keypad to enter the required concentration value. -
Page 27: Calibrating To A Factor
8.2.1 Calibrating to a Factor If a Factor has been entered, only a blank measurement is required. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. -
Page 28: Sample Measurement
Sample Measurement It is not possible to perform sample measurements before the instrument has been calibrated at the selected wavelength. 8.3.1 Measuring a Sample After Calibrating to a Factor Remove the blank solution by cleaning the upper and lower read heads. Pipette the sample to be analysed onto the lower read head and close the lid. -
Page 29: Section 9 - Spectrum
Section 9 - Spectrum The spectrum measurement mode enables measurements of absorbance or % transmittance over a range of wavelengths to be performed. The absorbance or % transmittance at each wavelength is plotted graphically. Post measurement tools such as peaks and valleys analysis and area under the graph can be performed. This operating mode can be used to partially characterise a sample. -
Page 30: Setting The Scan Interval
9.1.2 Setting the Scan Interval This function enables the interval between wavelengths measured in the spectrum scan to be set. The scan interval can be altered to 1, 2, 5 or 10nm by touching the value below scan interval . Select the required scan interval from the available options. -
Page 31: Data Analysis
Once the spectrum scan is completed it is possible to analyse the spectrum scan. Post measurement tools include peaks and valleys and area under the curve. To analyse the data touch Data Analysis Touching the spectrum scan will open a sliding cursor . -
Page 32: Area Under Curve
9.4.2 Area Under Curve To view the area under the curve touch . The default mode is baseline. To change this between baseline and tangent, touch to select the required measurement mode. Repeat touches will cycle between the two options. 9.4.2.1 Area Under Curve - Baseline Mode Baseline will calculate the area under the curve between the two sliding cursors... -
Page 33: Area Under Curve - Tangent Mode
9.4.2.2 Area Under Curve - Tangent Mode Tangent will calculate the area under the curve from the point where each of the two sliding cursors crosses the spectrum scan. Slide the cursors to the select the area required. You can also use the arrows to move the selected area. -
Page 34: Section 10 - Quantitation
Section 10 - Quantitation The quantitation measurement mode enables sample concentrations to be calculated using a standard curve. In this mode a number of standard solutions covering a range of known concentrations are measured at a set wavelength. The absorbance or % transmittance of these solutions is plotted to create a standard curve. Once the standard curve has been created a sample of unknown concentration can be measured and the concentration calculated using the standard curve. -
Page 35: Selecting Number Of Replicates
10.1.2 Selecting Number of Replicates To select the number of repeat measurements of a calibration standard touch . Touch the circle adjacent to the required unit of replicates to apply and return to the method set up. If 2 or more replicates are selected, becomes active. -
Page 36: Measuring Calibration Standards
10.2 Measuring Calibration Standards The measured standards are used to create a calibration curve. If there is only one standard available the concentration measurement mode should be used. Touch to add the first standard. Touch to use keypad to enter the concentration value required for that standard. Touch to apply. -
Page 37: Standard Curve
Touch to save the absorbance results for the 1st standard. Touch to add another standard and use the keypad to enter the concentration value required. This time a blank measurement is not required so is active straight away. Pipette the 2nd known standard solution onto the lower read head and close the lid. -
Page 38: Sample Measurement
Specific points can be selected on the graph by touching the graph, a sliding cursor will appear. It is possible to move the cursor by dragging left or right. The curve fit algorithm can be changed by touching . Select between linear through zero, linear, quadratic through zero and quadratic. The curve statistics are also displayed for the curve fit chosen. For example if the curve fit is y = mx+c the curve statistics displayed will be the gradient of the line (m), constant (c) and correlation coefficient (r²). Once all the standards have been measured touch and then the unknown samples can be measured. 10.4 Sample Measurement Ensure the upper and lower read heads are clean and pipette the sample to be measured onto the lower read... -
Page 39: Section 11 - Kinetics
Section 11 - Kinetics The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be measured over a set time; for example, enzyme activity. The absorbance or % transmittance is measured at regular time intervals at one wavelength over time. The results are plotted on a graph to show the change in absorbance or % transmittance over time. -
Page 40: Setting The Kinetics Measurement Time
11.1.2 Setting the Kinetics Measurement Time To set the total kinetics measurement time touch and enter the required run time. Scroll up or down beneath Hours, Minutes, Seconds to select the required time and touch to apply. Touch to exit the run time set up without saving the changes. -
Page 41: Blank Measurement
11.2 Blank Measurement A blank measurement must be performed at the same wavelengths at which the sample will be measured. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. 11.3 Sample Measurement Once a blank measurement has been performed... - Page 42 If a lag time has been set the instrument will count down the lag time before the kinetics run starts. If no lag time has been set the kinetics run starts straight away and a live kinetics run is shown on the screen. If the kinetics run needs to be stopped touch . A warning message will appear asking for confirmation to stop the kinetics run.
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Page 43: Data Analysis
11.4 Data Analysis Following the completion of the kinetics measurements it is possible to analyse the data. These include the rate of change and end point concentration. To analyse the data touch The rate of change of absorbance over time can be viewed for the entire kinetics run or for selected parts of the kinetics run. -
Page 44: Section 12 - Multi-Wavelength
Section 12 - Multi-Wavelength The multi-wavelength measurement mode enables measurements of absorbance and % transmittance to be performed, as well as concentration and ratios to be calculated. The sample can be measured at four different wavelengths and at one point in time. Touch the Multi-wavelength icon on the main menu to enter this measurement mode. -
Page 45: Equation Parameters
12.1.2 Equation Parameters To select the required equation parameters, touch the The type of equation can be selected from several options. Touch and select the required equation from the menu. If no equation is selected then the factor and units options will be disabled. Touch to apply the equation parameters or touch to return to the method set up without saving any... -
Page 46: 12.1.2.1 Entering A Factor
12.1.2.1 Entering a Factor If the equation selected requires Factors to calculate the concentration result, the factors will also need to be entered. Touch and use the keypad to enter the required factor. Touch to apply the entered factor. Touch to apply the factor or touch to return to the method set up without saving any changes. -
Page 47: Blank Measurement
12.2 Blank Measurement A blank measurement must be performed at the same wavelengths at which the sample will be measured. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. 12.3 Sample Measurement Once a blank measurement has been performed... -
Page 48: Section 13 - Nucleic Acid Modes
Section 13 - Nucleic Acid Modes There are three Nucleic Acid modes to choose from on the home screen. Jenway recommends that users select the required path length before the start of each experiment. See Section 5 - Micro Volume Settings for more information. 13.1 dsDNA Based on Multi-Wavelength mode. -
Page 49: Method Set Up
13.4 Method Set up The parameters which can be entered in this measurement mode are wavelength, type of equation*, dilution volume, sample volume. *If you choose the dsDNA with correction for protein contamination equation, you can enter factors if required. See 8.4.2 Equation Parameters for more information. -
Page 50: 13.4.2.1 Entering A Dilution Volume And Sample Volume
The equation parameters can be adjusted. From the method set up screen touch . To change the equation touch Select the required equation from the menu. 13.4.2.1 Entering a Dilution Volume and Sample Volume If the equation requires dilution volume and sample volume, they will need to be entered. Touch and use the keypad to enter the required values. -
Page 51: Blank Measurement
13.5 Blank Measurement A blank measurement must be performed at the same wavelengths at which the sample will be measured. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. 13.6 Sample Measurement Once a blank measurement has been performed... -
Page 52: Section 14 - Protein Modes
Section 14 - Protein Modes There are two Protein modes to choose from on the home screen. Jenway recommends that users select the required path length before the start of each experiment. See Section 5 - Micro Volume Settings for more information. 14.1 Direct UV Based on Multi-Wavelength mode. -
Page 53: Method Set Up
14.3 Method Set up The parameters which can be entered in this measurement mode are wavelength, dilution volume, sample volume. 14.3.1 Selecting a Wavelength The wavelength value can be adjusted by touching and then using the keypad to enter the required wavelength. To add background correction touch , to remove touch 14.3.2... -
Page 54: Blank Measurement
14.4 Blank Measurement A blank measurement must be performed at the same wavelengths at which the sample will be measured. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch and the instrument will calibrate to zero absorbance and 100% transmittance. 14.5 Sample Measurement Once a blank measurement has been performed... -
Page 55: Section 15 - Colorimetric Protein Assays
Section 15 - Colorimetric Protein Assays There are five Colorimetric Protein Assays modes to choose from on the home screen. Jenway recommends that users select the required path length before the start of each experiment. See Section 5 - Micro Volume Settings for more information. All of the five colorimetric protein assays modes are based on the Quantitation mode and differ only in the wavelength they use. -
Page 56: Method Set Up
15.6 Method Set up The parameters which can be entered in this measurement mode are wavelength, number of replicates for the calibration standards and concentration units for the calibration standards. 15.6.1 Selecting a Wavelength The wavelength value can be adjusted by touching and using the keypad to enter the required wavelength. -
Page 57: Selecting Concentration Units
15.6.3 Selecting Concentration Units The units of concentration can be selected from several options. Touch to select from the menu. Touch the circle adjacent to the required unit of concentration. The selected unit will be displayed against the final concentration result. Once the method parameters have been entered touch to start measuring the calibration standards. Touch to return to the home screen. 15.7 Measuring Calibration Standards The measured standards are used to create a calibration curve. -
Page 58: Before The 1St Standard Can Be Measured You Will Need To Perform A Blank Measurement. Open The
Touch and use the keypad to enter the concentration value required for that standard. Touch to confirm. Before the 1st standard can be measured you will need to perform a blank measurement. Open the spectrophotometer lid and pipette the blank solution onto the lower read head and close the lid. Touch the instrument will calibrate to zero absorbance and 100% transmittance. -
Page 59: Standard Curve
Touch to add another standard and use the keypad to enter the concentration value required. This time a blank measurement is not required so is active straight away. Pipette the 2nd known standard solution onto the lower read head and close the lid. Touch to measure the standard. -
Page 60: Sample Measurement
15.9 Sample Measurement Ensure the upper and lower read heads are clean and pipette the sample to be measured onto the lower read head, close the lid and touch Once the measurement is complete the results will be shown on the screen. Touch to measure subsequent samples in the same way. -
Page 61: Section 16 - Saving, Loading, Deleting And Printing
Section 16 - Saving, Loading, Deleting and Printing 16.1 Saving Methods 16.1.1 Saving Methods to Internal Memory On each method set up screen there is an overflow icon . Touch and then touch to save the entered method parameters. Use the keypad to enter the method name and touch Save to apply the name, touch 16.1.2 Saving Methods to CPLive... -
Page 62: Saving Results
16.4 Saving Results 16.4.1 Saving Results to Internal Memory After a measurement has been performed, touch 16.4.2 Saving Results to CPLive You can save your results to CPLive via the home or results screen. On the home screen touch . Touch , you can then select each result individually by touching by the side of each method or touch to select all the methods. Touch the overflow icon... -
Page 63: Section 17 - Accessories And Spare Parts
Section 17 - Accessories and Spare Parts 17.1 Optional Accessories Please visit www.jenway.com for a full list of available accessories. 17.2 Spare Parts Please contact your local sales specialist or email cpspares@coleparmer to enquire about available spares. Section 18 - Maintenance and Servicing WARNING: Before attempting any maintenance, servicing or cleaning, ensure that the equipment has been allowed to cool down. -
Page 64: Read Head Re-Conditioning
18.1.3 Read Head Re-conditioning Reagents containing surfactants can “un-condition” the measurement read head surfaces so that the liquid does not form a stable sample droplet. If this occurs, “buff” the read head surfaces by rubbing each measurement surface firmly with a dry laboratory wipe 30-40 times. This will “re-condition” the surface allowing the sample droplet to form. -
Page 65: Service, Repairs And Support
18.2 Service, Repairs and Support Any service, repairs or replacement of parts MUST be undertaken by suitably qualified personnel. Only spare parts supplied or specified by Cole-Parmer or its agents should be used. Fitting of non-approved parts may affect the performance and safety features designed into the instrument. For a comprehensive list of parts required by service engineers conducting internal repairs please contact the service department quoting the model and serial number: Email: cpservice@coleparmer.com Tel: +44 (0)1785 810475... -
Page 66: Section 20 - Calibration
Section 20 - Calibration Jenway recommend that the micro volume accessory is calibrated every 6 months. A set of calibration solutions is available to order (part code 035 092). Please note that the calibration solutions should be discarded 1 week after being opened. -
Page 67: Step 1 - Air
Enter the values from the calibration certificate that is supplied with the calibration solution set. Touch 20.2.1 Step 1 - Air Touch , when the instrument finishes touch to move onto the next calibration step. 20.2.2 Step 2 - Blank Pipette matrix blank solution (white label) onto the lower read head, close the lid and touch . When the instrument finishes, clean the upper and lower read heads and touch to move onto the next calibration step. -
Page 68: Step 3 - Standard 1
20.2.3 Step 3 - Standard 1 Pipette 10x ref calibration standard (blue label) onto the lower read head, close the lid and touch . When the instrument finishes, clean the upper and lower read heads and touch to move onto the next calibration step. 20.2.4 Step 4 - Standard 2 Pipette 10x ref calibration standard (blue label) onto the lower read head, close the lid and touch . -
Page 69: Step 6 - Verification: Blank
20.2.6 Step 6 - Verification: Blank Pipette matrix blank solution (white label) onto the lower read head, close the lid and touch . When the instrument finishes, clean the upper and lower read heads and touch to move onto the next calibration step. 20.2.7 Step 7 - Verification: Standard Pipette 10x ref calibration standard (blue label) onto the lower read head, close the lid and touch . -
Page 70: Section 21 - Technical Specification
Section 21 - Technical Specification 21.1 General Specification 7415 Nano Wavelength Range 198 to 1000nm Resolution 1.0nm Wavelength Accuracy ± 2.0nm Spectral Bandwidth 5.0nm Path Length 0.2 or 0.5mm (user-selected) Absorbance Range - 15 to 125A (10mm equivalent) Absorbance Accuracy ±... -
Page 71: Weights And Dimensions
21.2 Weights and Dimensions Weight 9.0kg 280mm 500mm... -
Page 72: Section 22 - Troubleshooting
Section 22 - Troubleshooting During initial power on self-test (POST) The following errors can appear during the initial self-test. A hardware problem has been detected. You may continue to use the instrument but calibration data may have been affected causing any readings to be inaccurate. Contact Cole-Parmer support and quote error code 101. A hardware problem has been detected. - Page 73 During scanning (blank, standard or sample) The following errors can appear whenever the optical hardware is used. Each error message will be attached to the activity which was just performed. The error messages are displayed as a notification on screen with an option to display more information about the error. If multiple errors have been detected, they will be displayed in the more information. The ‘blank / standard / sample’...
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Page 74: Section 23 - Glossary Of Icons
Section 23 - Glossary of Icons Sample warning Lowry Overflow Analysis Pierce Accessory attached Area under curve Analysis Back arrow Printer Peaks and valleys Upload to CPLive Blank Take reading Concentration Blank complete Save Kinetics Apply Search Multi-wavelength Select files Settings Photometrics Cancel Spectrum peak... -
Page 75: Section 24 - Chemical Compatability
Section 24 - Chemical Compatability Assay Chemical Concentration Sodium bicinchoninate Sodium carbonate Sodium tartrate 0.16%* Sodium hydroxide 0.1M* Sodium bicarbonate 0.95%* Copper (II) sulphate 0.08% Biuret Sodium potassium tartrate 0.9%* Biuret Copper (II) sulphate 0.3%* Biuret Potassium iodide 0.5%* Biuret Sodium hydroxide 0.08M Lowry... -
Page 76: Index
Index Index Accessories and Spare Parts ..........63 Deleting Methods ..............61 Adding a Sample ..............22 Deleting Results ..............62 Adding, Removing and Recovering Samples ......22 Direct UV ................52 Blank Measurement ............54 Entering a Dilution Volume and Sample Volume ..53 BCA ..................55 Entering a Factor ............53 Biuret ...................55 Equation Parameters ...........53 Bradford ................55... - Page 77 Optional Accessories ............63 Kinetics ................39 Blank Measurement ............41 Overview ................10 Data Analysis ..............43 End Point Concentration ..........40 Packaging Material ..............65 Method Set up ............39 Photometrics ................23 Sample Measurement..........41 Blank Measurement ............24 Selecting Absorbance or % Transmittance ....40 Method Set up ............23 Selecting a Wavelength ..........39 Sample Measurement..........24 Setting Lag Time ............40...
- Page 78 Saving, Loading, Deleting and Printing .........61 Technical Specification ............70 Saving Methods ..............61 Theory and Practice of Spectroscopy Measurement ....11 Saving Methods to CPLive ...........61 Theory of Spectroscopy Measurement ........11 Saving Methods to Internal Memory ......61 Troubleshooting ..............72 Saving Methods to USB Memory Stick ......61 During initial power on self-test (POST)......72 During scanning (blank, standard or sample) ....73 Saving Results ..............62...
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Page 79: Declaration Of Conformity
Declaration of Conformity This product meets applicable and so we cannot guarantee that interference will not harmonized standards for radio frequency occur in practice. Where there is a possibility that injury, interference and may be expected not to damage or loss might occur if equipment malfunctions interfere with, or be affected by, other equipment with due to radio frequency interference, or for general similar qualifications. - Page 80 Cole-Parmer Ltd. Beacon Road, Stone, Staffordshire, ST15 0SA, United Kingdom Tel: +44 (0)1785 812121 Email: cpinfo@coleparmer.com Web: www.jenway.com...
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