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Summary of Contents for Cole Parmer 1100 Series
- Page 1 Cole Parmer 1100 SERIES SPECTROPHOTOMETER USER’S MANUAL Cole Parmer Instruments Company 625 East Bunker Court Vernon Hills, IL 60061 800-323-4340...
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Page 2: Table Of Contents
TABLE OF CONTENTS Page number Safety, General Information ............Introduction .............…………………..… Working Principle ................Unpacking Instructions ...............… Specifications …................Installation ..................Components of 1100, 1100RS (backside) Basic Operation ................Sample Preparation and Analysis ..........Additional Features of 1100RS Concentration Mode ……………………..…………………..Factor Mode ………………………….………………………... -
Page 3: Safety, General Information
Safety This apparatus has been designed and tested in accordance with EN 61010 Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, and has been supplied in a safe condition. The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. -
Page 4: Introduction
The protection is likely to be impaired if, for example, the apparatus • Shows visible damage • Fails to perform the intended measurements • Has been subjected to prolonged storage under unfavorable conditions • Has been subjected to severe transport stresses Performance To ensure that the instrument is working within its specification, especially when making measurements of an important nature, carry out performance checks with particular... -
Page 5: Unpacking Instructions
Block diagram for the Spectrophotometer 100%T Light Mono- Sample Detector Display Source chromator Compartment In your spectrophotometer, light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating. The grating disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of the monochromator by a collimating mirror. -
Page 6: Specifications
Specifications Model 1100 Model 1100RS Wavelength Range 335-1000nm 335-1000nm Spectral Bandpass 20nm 10nm Wavelength Accuracy Better than 2nm Better than 1nm Wavelength Repeatability Better than 1nm Better than 1nm Stray Radiant Energy <0.5 @ 340 and 400nm <0.5 @ 340 and 400nm Photometric Range 0 to 125%T 0 to 125%T... -
Page 7: Basic Operation
Basic Operation: Simple operating instructions are printed on the front panel of your Cole-Parmer 1100/1100RS. Prepare the spectrophotometer Turn on the spectrophotometer by pressing the Power Switch (IO). Allow 15 minutes for the instrument to warm up. Select either the %Transmittance or Absorbance operating mode by pressing the %T/A selector button (MODE) until the red light for T or A is on. -
Page 8: Additional Features Of 1100Rs
Set 0.000A or 100%T with the (0A/100%T) control button. NOTE: This step fulfills the instruction on the front of the spectrophotometer…(Set Full Scale). Remove the blank cuvette or test tube. Set it aside in the case that you may need to adjust the (0A/100%T) control button later (i.e. change the wavelength). Analyze Sample Rinse a second cuvette with a small amount of the sample solution to be tested. -
Page 9: Factor Mode
displayed. In this case, divide the concentration by 10, re-select the C mode by successive presses on the Mode button, cycling through the F, %T, and A modes, and follow step 2 above to set the concentration of the standard to the reduced value. 8. -
Page 10: Model 1100 Nomenclature
Model 1100 Spectrophotometer: Mode Indicator: Allows the operator to know the measurement mode currently in use (%Transmittance, Absorbance). MODE Button: Switches between %Transmittance and Absorbance operating modes at any time of measurement. 0A/100%T Button: Adjusts digital readout to 100%T or 0.000A when blank reference solution is in sample compartment. -
Page 11: Model 1100Rs Nomenclature
Model 1100RS Spectrophotometer: Mode Indicator: Allows the operator to know the measurement mode currently in use (%Transmittance, Absorbance). MODE Button: Switches between %Transmittance and Absorbance operating modes at any time of measurement. 0A/100%T Button: Adjusts digital readout to 100%T or 0.000A when blank reference solution is in sample compartment. -
Page 12: Maintenance
6. Re-install the grill plate. Wavelength Calibration: Normally the Cole-Parmer 1100 Series spectrophotometer retains its wavelength calibration indefinitely. However if the instrument receives a severe shock or is abused, use the following methods to check wavelength calibration. Please note that this test requires the Cole-Parmer Didymium filter, p/n 1100-110, or the Holmium Oxide filter, p/n 1100-109. - Page 13 2. Select the Absorbance operating mode. 2. Set the wavelength to 350nm. 3. Make sure the cuvette holder is empty and place it in the sample compartment. Close the sample compartment lid. 4. Set zero Absorbance by pressing the 0A/100%T. Wait a few seconds while the display flashes ‘BLA’.
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Page 14: Absorbance Accuracy Checks
10. Make sure the cuvette holder is empty and place it in the sample compartment. Close the sample lid. 11. Set zero Abs by pressing the 0A/100%T button. Wait a few seconds while the display flashes ‘BLA’. The reading should then be 0.000A. If not repeat step 11. 12. -
Page 15: Stray Light Check
Put the cuvette in the adapter into the sample compartment and close the lid. Read the absorbance of the standard from the display. The value should be 1.288 + 0.04A. Refer to the notes above when interpreting the result. Stray Light Check Specification: Less than 0.5%T at 340nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows:... -
Page 16: Troubleshooting
Trouble Shooting: PROBLEM Possible Cause Solution Instrument Inoperative Power cord not connected Plug instrument in. to outlet Dead Power outlet Change to a different outlet Internal fuse blown or Call an authorized service defective electronic engineer. component Light beam blocked Check sample holder. - Page 17 Problem Possible Cause Solution Digital Display does not change Wrong wavelength setting. Check sample procedure and wavelength setting. regardless of sample concentration (continued…) Insufficient sample Fill cuvette with more volume. sample. Stray sample preparation Prepare the sample away vapors. from the instrument. Use proper ventilation.
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