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Summary of Contents for Cole Parmer SP-200-VIS
- Page 1 Cole-Parmer ® SP-200-VIS SP-250-VIS Visible Spectrophotometer 630 026-CPB REV K...
- Page 3 Ces informations sont importantes ; veuillez les lire avec attention avant d'installer ou d'utiliser l'instrument. 1. Les spectrophotom•tres SP-200-VIS et SP-250-VIS sont con•us pour •tre utilisŽs par du personnel formŽ qui conna”t les principes et les applications concernŽs. Pour obtenir de l'aide et des conseils supplŽmentaires, merci de consulter le site Internet...
- Page 4 Dies sind wichtige Informationen, bitte lesen Sie sie vor Installation oder Verwendung dieses GerŠts sorgfŠltig durch. 1. Die Spektrophotometer SP-200-VIS und SP-250-VIS sind fŸr den Betrieb durch geschultes Personal, das Ÿ ber die entsprechenden Prinzipien und Anwendungen Bescheid wei§, vorgesehen. Weitere Hilfe und Hinweise finden Sie auf www.Cole-Parmer.de...
- Page 5 Informaci—n importante que debe leer atentamente antes de instalar o utilizar este instrumento. 1. Los espectr—metros SP-200-VIS y SP-250-VIS se han concebido para ser utilizados por personal con formaci—n que conozca los principios y las aplicaciones que entra–an. Si necesita m‡s ayuda o indicaciones, dir’jase a www.Cole-Parmer.com...
- Page 6 Queste informazioni sono importanti; si prega di leggerle attentamente prima di installare o utilizzare lo strumento. 1. Gli spettrofotometri SP-200-VIS e SP-250-VIS sono progettati per essere utilizzati da personale qualificato che conosce i principi e le applicazioni ad essi collegati. Per ulteriori informazioni e per assistenza visitare il sito internet www.Cole-Parmer.com...
- Page 7 eseguire misurazioni e analisi con questo strumento. Attrezzature idonee per la protezione personale e la sicurezza vanno utilizzate in ogni circostanza. 9. Se si sospetta che la protezione della sicurezza sia stata compromessa in qualsiasi modo, disattivare e rendere inutilizzabile lo spettrofotometro. La condizione di guasto deve essere comunicata al responsabile della manutenzione appropriato.
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Page 8: Table Of Contents
Contents SECTION 1 - Introduction ..................INSTRUMENT DESCRIPTION .................... INSTRUMENT SPECIFICATION ..................9 SECTION 2 Ð Installation ..................10 UNPACKING ........................10 INSTALLATION ........................10 DISPLAY ..........................11 CONTROLS ........................11 REAR PANEL ........................12 SAMPLING ......................... 13 SECTION 3 Ð Theory and Practice of Spectroscopy Measurements ....... 14 THEORY OF SPECTROSCOPY MEASUREMENT ............ -
Page 9: Section 1 - Introduction
SECTION 1 - Introduction INSTRUMENT DESCRIPTION The SP-200-VIS and SP-250-VIS spectrophotometers are suited to a wide range of applications in education, quality control, environmental clinical analysis. Both models visible spectrophotometers covering a wavelength range from 320nm to 1000nm, with measurement modes for absorbance, % transmittance and concentration. These... -
Page 10: Unpacking
2. Required power cable either UK mains lead moulded plug (HH179(S)), EU mains lead moulded plug (HH180(S)), or US mains lead moulded plug (FCABLEUS) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084), model SP-200-VIS only 4. Cole-Parmer 63-zero PC software (635 100) and interface cable (013 210) model SP-200-VIS only 5. -
Page 11: Display
The instrument will automatically re-align the monochromator at zero order wavelength. During this initialisation, the instrument displays CAL on the primary display before displaying the main measurement screen. Please note that the instrument will go to the last wavelength and measurement mode used. -
Page 12: Rear Panel
Fig. 2.4.1 Ð Controls 1. Arrow Keys: Used to adjust values on the selected display 2. Arrow Keys: Used to move the cursor horizontally between menu options 3. Return Key: Used to select the display menu option 4. CAL Key: Initiates a calibration routine 5. -
Page 13: Sampling
5. RS232 serial port Connection to a PC SAMPLING Model SP-200-VIS is supplied with a 10mm cuvette holder. Other accessories can be fitted (refer to section 7). Model SP-250-VIS is supplied with a dual 10mm cuvette holder with a 12.7mm test tube holder. -
Page 14: Section 3 - Theory And Practice Of Spectroscopy Measurements
SECTION 3 – Theory and Practice of Spectroscopy Measurements THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. -
Page 15: Spectroscopy Measurement
The optical layout of the SP-200-VIS and SP-250-VIS spectrophotometers is shown below: Figure 3.2.1 Ð Diagram of light path The light from the pre-focused tungsten halogen lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths. -
Page 16: Good Practice Guidelines
GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme. - Page 17 h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used. 5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible.
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Page 18: Section 4 Ð Photometrics Measurements
SECTION 4 Ð PHOTOMETRICS MEASUREMENTS The SP-200-VIS/SP-250-VIS can perform simple measurements of % transmittance or absorbance. The sample is measured at one wavelength and at one point in time. TRANSMISSION MEASUREMENT MODE To select % Transmittance use the LEFT or RIGHT arrow keys to move the cursor to the %T indicator on the menu bar (refer to figure 2.3.1). -
Page 19: Section 5 Ð Concentration Measurements
SECTION 5 Ð CONCENTRATION MEASUREMENTS The concentration measurement mode enables simple measurements of concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. -
Page 20: Section 6 Ð Connecting To A Pc
A printout is initiated by pressing the PRINT key. If the sample number is unity, then the printout will include a header block. The sample number is incremented every time the PRINT key is pressed. The following commands can also be sent to the SP-200-VIS and SP-250-VIS via the serial interface (using Windows HyperTerminal, for example):... -
Page 21: Rs232 Output
Ð LINKED TO RTS CTS 5 Ð OUTPUT FROM SP-200-VIS/SP-250-VIS DSR 6 Ð OUTPUT FROM SP-200-VIS/SP-250-VIS DCD 8 Ð INPUT TO SP-200-VIS/SP-250-VIS (must be active) DTR 20 GND 7 Suggested interconnections are detailed below: SP-200-VIS/SP-250-VIS IBM PC XT (25 way ÒDÓ) TDX 2... -
Page 22: Recorder Output
SP-200-VIS/SP-250-VIS IBM PC XT (9 way ÒDÓ) TDX 2 3 TXD (From PC) RXD 3 2 RXD (To PC) RTS 4 7 RTS (From PC) CTS 5 8 CTS (To PC) DSR 6 6 DSR (To PC) DCD 8 1 DCD (To PC) -
Page 23: Section 7 - Accessories And Spare Parts
Interface cable kit 542 009 7.2 SPARES Description of Spare Part Part Code Tungsten halogen lamp (SP-200-VIS & SP-250-VIS) 012 075 10x10mm path length cuvette holder 630 204 Dual cell holder for 10mm cuvettes and 12.7mm diameter tubes (SP-250-VIS ONLY) -
Page 24: Section 8 - Maintenance And Service
SECTION 8 – Maintenance and Service ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. - Page 25 For further instructions refer to the service manual. SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Cole-Parmer equipment develops a fault. Please contact them by one of the following means with a clear description of the problem: E-mail: cpservice@antylia.com Tel: +44 (0) 1785 810475...
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Page 26: Section 9 - Troubleshooting
Refer to the table below to diagnose error codes. If you require further assistance contact Cole- Parmer Help (refer to section 9.2). Error Code Error Name Issue This error occurs when the SP-200-VIS/SP-250-VIS closes Err 1 Dark cal error the shutter to block... - Page 27 This product meets the applicable CE Directives that interference will not occur in practise. Where and UKCA Legislation for radio frequency there is a possibility that injury, damage or loss might interference and may be expected not to occur if equipment malfunctions due to radio interfere with, or be affected by, other equipment with similar frequency interference, or for general advise before qualifications.
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Page 28: Ordering Information
Ordering Information Order Cole-Parmer Cole-Parmer Jenway Jenway Series Model Model Part No. 6300 Visible SP-200 SP-200-VIS-115 630531 Spectrophotometer 115V 6300 Visible 83054-05 SP-200 SP-200-VIS 630501 Spectrophotometer 230V 6320D Visible 83054-00 SP-200 SP-250-VIS-115 632621 Spectrophotometer 115V 6320D Visible 83054-07 SP-200 SP-250-VIS...
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