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Summary of Contents for CORNING Axygen Axypet AP-2
- Page 1 Axygen Axypet ® ® Pipettors Single-channel, 8-channel, A Corning Brand and 12-channel Pipettors Instruction Manual...
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Page 2: Table Of Contents
Table of Contents 1.0 Product Description ............1 2.0 Packaging ................1 3.0 Pipettor Design ..............2 4.0 Safety Recommendations ..........3 5.0 Specifications ................ 4 6.0 Pipettor Operation .............. 5 7.0 Operating Instructions ............9 8.0 Aspirating and Dispensing ..........10 9.0 Checking Pipetting Accuracy Parameters and Pipettor Recalibration ..........12 10.0 Pipettor Maintenance .............15... -
Page 3: Product Description
1.0 Product Description The Axygen® Axypet® single-channel pipettor is a volumetric instrument designed to measure and transfer liquids precisely and safely. It is available in volumes from 0.1 μL to 10,000 μL. Axygen Axypet 8-channel and 12-channel pipettors are designed for microplate filling. The pipettors enable precise and simultaneous delivery of 8 or 12 preset volumes of liquid. -
Page 4: Pipettor Design
Filters The 5,000 μL and 10,000 μL pipettors include a replaceable filter, fitted at the bottom of the shaft which prevents aspirated liquid from entering the shaft and contaminating the inner surface and plunger. Use of the filter is particularly important when aspirating and dispensing large volumes of liquid. The filter should be replaced if it becomes wet. 3.0 Pipettor Design Model Identification The volume range of the pipettor is shown on the pushbutton and on the tip ejector bar in multi-channel pipettors. -
Page 5: Safety Recommendations
D. Tip ejector: For multi-channel pipettors, the tips are ejected sequentially, reducing the required force. F. Shaft nut N. Ejector button R. Multi-channel manifold: Contains a set of plungers and a set of flexible suspension shafts, which function to reduce the force required to attach the tip. 4.0 Safety Recommendations Long-term use of the pipettor relies on the correct method of use. -
Page 6: Specifications
5.0 Specifications The pipettor is a high quality instrument which offers excellent accuracy and precision. The accuracy and precision (repeatability) of the liquid volume depends on the quality of pipette tips used. The values for accuracy and precision shown in the table below were obtained using manufacturer non-filter pipette tips. These tips are recommended for use to ensure compatibility, accuracy, and precision when pipetting. -
Page 7: Pipettor Operation
Max. ±1.0 ≤0.6 The accuracy and precision were obtained gravimetrically using Corning’s tips performing at least 10 measurements of distilled water at a temperature of 20°C ± 1°C according to EN ISO 8655 standards. The use of tips from other manufacturers or filter tips may result in incorrect liquid aspiration and require pipettor recalibration. - Page 8 ✓ ✕ ✓ ✕ NOTE: • Do not attach the tips with a rocking motion, as this may damage the shaft or plunger. Observe this rule particularly with single-channel pipettors of low volume range. • Never draw liquids directly into the pipettor without the tip attached.
- Page 9 2. Tip ejector adjustment • To increase the ejector length turn the plastic bush counterclockwise. • To decrease the ejector length turn the plastic bush clockwise. 3. Tip ejector assembly • Press the ejector button. • Line the tip ejector up with the plastic arbor. •...
- Page 10 Volume Setting 1. To enable volume selection, set the locking ring to the lower position. 2. Aspiration volume can be set by either turning the pipetting pushbutton knob, or the adjustment knob. The volume setting is displayed as a three digit counter, which should be read from top to bottom.
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Page 11: Operating Instructions
Nominal Volume Counter (µL) Readings Set Volume Increment Single-channel Pipettors (continued) 10,000 7.5 mL 20 μL 8-channel and 12-channel Pipettors 8 - 10 3.5 μL 0.02 μL 12 - 10 8 - 50 6.5 μL 0.10 μL 12 - 50 8 - 200 85.0 μL 0.20 μL... -
Page 12: Aspirating And Dispensing
• The pipettor should be held in a vertical position. • The pipette tip should be changed whenever the volume setting is altered, and when a different liquid is to be aspirated. • The pipette tip should be changed if a droplet remains on the end of the tip from the previous pipetting operation. - Page 13 Aspirating Liquid 1. Press the pushbutton to the first stop point. Holding the pipettor vertically, immerse the pipette tip into the sample liquid to the recommended depth (for recommended values see Section 7). If the pipette tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released, air may enter the pipette tip.
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Page 14: Checking Pipetting Accuracy Parameters And Pipettor Recalibration
It is recommended to record recalibration and correction values, in order to facilitate reverse calibration to a standard liquid. 9.0 Checking Pipetting Accuracy Parameters and Pipettor Recalibration The pipettors have been factory-calibrated using gravimetric methods with manufacturer pipette tips and distilled water, in accordance with EN ISO 8655 guidelines for the maximum (nominal) liquid volume drawn by the pipettor and for 10% of the maximum or minimum liquid... - Page 15 Volume Checked (V, µL) Balance Sensitivity (mg) 0.1≤V≤10 0.001 10≤V≤100 0.01 100≤V≤1,000 V>1,000 • When calculating the liquid volume aspirated by the pipettor, the conversion factor (Z) [μL/mg] for distilled water or a liquid with comparable density should be taken into account. Sample values of conversion factors are given in the following table.
- Page 16 Volume Change ΔV (μL) for a Nominal Preset Turn of a Calibration Key by Volume Volume Permissible (µL) (µL) Values (µL) 1 Turn 1 Increment 8-channel and 12-channel Pipettors 0.92 - 1.08 0.33 0.0137 4.8 - 5.2 1.67 0.070 19.4 - 20.6 6.30 0.262 49.2 - 50.8...
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Page 17: Pipettor Maintenance
When pipetting liquids with physical properties considerably different from those of water, follow the guidance given in Section 5. 10.0 Pipettor Maintenance Depending on the applications and intensity of use, the pipettor requires periodic maintenance. The components exposed to corrosive vapors, such as shaft elements, should be regularly checked and cleaned. -
Page 18: Troubleshooting
Ultra Violet (UV) Sterilization The outer body of the pipettor is UV-resistant. The recommended distance from the radiation source to exposed element should not be less than 50 cm. Prolonged or intense UV exposure can cause discoloration of pipettor parts but does not affect its performance. 11.0 Troubleshooting If a problem is encountered during pipettor operation, use the following table to identify and eliminate the fault... -
Page 19: Spare Parts
12.0 Spare Parts Contact Corning Customer Service to inquire about availability of spare parts. Pipettor model and name of the part required should be specified. The most common parts are depicted below. - Page 20 Item Description Model Cat. No. Qty/Pk Volume adjustment SP29327 knob set C, I, J* Shaft with O-ring SP19111 and seal (grey) SP19112 SP19113 50, 100 SP19114 200, 250 SP19115 1,000 SP19116 5,000 SP19118 10,000 SP19119 Tip ejector 2, 10 SP19051 20, 50, 100 SP19052 200, 250...
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Page 21: Limited Warranty
Corning of any such defect. Corning is not liable for any incidental or consequential damages, commercial loss, or any other damages from the use of this product. - Page 22 This warranty gives you specific legal rights. You may have other rights which vary from state to state. No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty. For your reference, make a note of the model number, serial number, date of purchase, and supplier here.
- Page 23 *For a listing of US medical devices, regulatory classifications or specific information on claims, visit www.corning.com/resources. Corning’s products are not specifically designed and tested for diagnostic testing. Many Corning products, though not specific for diagnostic testing, can be used in the workflow and preparation of the test at the customers discretion. Customers may use these products to support their claims.
- Page 24 55 (11) 3089-7400 t 65 6572-9740 Mexico Taiwan t (52-81) 8158-8400 t 886 2-2716-0338 For a listing of trademarks, visit www.corning.com/trademarks. All other trademarks are the property of their respective owners. © 2022, 2025 Corning Incorporated. All rights reserved. 3/25 CLS-A-EQ-015DOC REV3...
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