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Summary of Contents for CORNING Axygen Axypet
- Page 1 Axygen® Axypet® Pipettors Single-channel, 8-channel, A Corning Brand and 12-channel Pipettors Instruction Manual...
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Page 2: Table Of Contents
Table of Contents 1. Product Description .............. 1 2. Setting the Volume ..............5 3. Aspiration and Dispensing Instructions ......8 4. Pre-rinsing ................9 5. Dense and Viscous Liquids ..........10 6. Axygen® Pipet Tips ...............10 7. Recommendations ...............10 8. Recalibration .................11 9. -
Page 3: Product Description
It can accommodate volumes from 0.1 μL to 10,000 μL depending on the model. Axygen Axypet 8-channel and 12-channel pipettors have been designed for microplate filling. The pipettors enable precise and simultaneous delivery of 8 or 12 preset volume doses of liquid. - Page 4 The “H“ spacers included with each pipettor, allow the ejector length to be adjusted by +1 mm or +2 mm. An ”H0” spacer is fitted as standard. The outside shape of the spacer identifies the adjustment. 2 | AXYGEN AXYPET PIPETTORS...
- Page 5 5,000 and 10,000 μL Pipettors The length of tip ejector is adjusted by screwing in or screwing out the tip ejector arbor with a small screwdriver. Turn the screwdriver counterclockwise to increase the length of the tip ejector, and clockwise to reduce the length of the tip ejector.
- Page 6 ±0.8 ≤0.24 Max. ±0.8 ≤0.20 Min. ±1.2 ≤0.60 AP-200 ±0.8 ≤0.25 Max. ±0.6 ≤0.20 Min. ±1.0 ≤0.40 AP-250 ±0.8 ≤0.30 Max. ±0.6 ≤0.30 Min. ±1.6 ≤0.40 1,000 AP-1000 ±0.7 ≤0.20 1,000 Max. 1,000 ±0.6 ≤0.15 4 | AXYGEN AXYPET PIPETTORS...
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Page 7: Setting The Volume
Model Fit to Tips Volume Without Range Volume Accuracy Precision Filter (μL) Cat. No. (μL) (μL) Single-channel Pipettors (continued) Min. 1,000 ±0.6 ≤0.25 5,000 AP-5000 2,500 ±0.6 ≤0.20 5,000 Max. 5,000 ±0.5 ≤0.15 Min. 1,000 ±2.5 ≤0.60 10,000 AP-10ML 5,000 ±0.8 ≤0.30 10,000... - Page 8 12.5 μL 0.02 μL 38.0 μL 0.2 μL 75.0 μL 0.2 μL 125.0 μL 0.2 μL 245.0 μL 0.2 μL 1,000 750.0 μL 2 μL 5,000 1.25 mL 10 μL 10,000 7.5 mL 20 μL 6 | AXYGEN AXYPET PIPETTORS...
- Page 9 Model Volume Counter Range (μL) Readings Volume Increment 8-channel and 12-channel Pipettors 8 - 10 3.5 μL 0.02 μL 12 - 10 8 - 50 6.5 μL 0.10 μL 12 - 50 8 - 200 85.0 μL 0.20 μL 12 - 200 8 - 300 250.0 μL 1.0 μL...
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Page 10: Aspiration And Dispensing Instructions
Model Volume Range (μL) Immersion Depth (mm) 2, 10 ≤1 20, 50, 100 2 - 3 200, 250, 300, 1,000 2 - 4 5,000 3 - 6 10,000 5 - 7 8 | AXYGEN AXYPET PIPETTORS... -
Page 11: Pre-Rinsing
2. Release the pipetting pushbutton slowly and smoothly to aspirate the sample. Wait one second and then withdraw the pipet tip from the liquid. If the pipet tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released, air may enter the disposable pipet tip. -
Page 12: Dense And Viscous Liquids
• Make sure to operate the pipettor slowly and smoothly. • The depth of immersion in the sample liquid should be the minimum necessary and should remain constant during aspiration. 10 | AXYGEN AXYPET PIPETTORS... -
Page 13: Recalibration
• The pipettor should be held in a vertical position. • Change the pipet tip when volume setting is changed or when a different liquid is to be aspirated. • Change the pipet tip if a droplet remains on the end of the tip from the previous pipetting operation. - Page 14 9.84 - 10.16 2.50 20 - 200 19.76 - 6.30 20.24 50 - 250 49.5 - 50.5 6.30 1,000 100 - 1,000 98.4 - 101.6 25.00 5,000 1,000 - 5,000 1,000 994 - 1,006 125.00 12 | AXYGEN AXYPET PIPETTORS...
- Page 15 Volume Change Model ΔV for Full Turn of Volume Range of Preset Permissible the Calibration Key Range Pipettor Volume Volumes (24 Increments) (μL) Volumes (μL) (μL) (μL) (μL) 10,000 1,000 - 1,000 975 - 1,025 250.00 10,000 8-channel and 12-channel Pipettors 8 - 10 0.5 - 10 0.92 - 1.08...
- Page 16 4. Take out the calibration key and fix the pipetting pushbutton. The pipetting pushbutton should be fixed by placing the lower section on the arbor first (A2) and then the button (A1). Determine the average aspirated volume. The average 14 | AXYGEN AXYPET PIPETTORS...
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Page 17: Troubleshooting
volume should be within the permissible range shown in the table. If the volume exceeds the values stated, the recalibration procedure should be repeated. When pipetting liquids with physical properties that are considerably different from those of water, follow the rules as described in Section 5. - Page 18 To remove the tip ejector in models AP-5000 and AP-10ML, remove the ejector pushbutton and using a screwdriver unscrew the tip ejector by turning the screwdriver counterclockwise. 16 | AXYGEN AXYPET PIPETTORS...
- Page 19 If the problem continues after carrying out the above steps, contact your Corning representative. Before returning the pipettor, please ensure the pipettor is completely free of any chemical, radioactive, or microbiological contamination which could pose a threat during transport and repair.
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Page 20: Cleaning And Sterilization
The pipettors are UV-resistant. However, the recommended distance from the radiation source to the exposed element should be not less than 50 cm. Prolonged and very intense UV exposure can cause decoloration of pipettor parts without effecting their performance. 18 | AXYGEN AXYPET PIPETTORS... -
Page 21: Components
11. Components The following components are supplied with the pipettor: • Instruction manual • Calibration key • Ejector length adjustment spacers (for single-channel pipettors) • Ejector cap (for pipettor models AP-2, AP-10, AP-5000, AP-10ML) • Identification labels • Filters (for pipettor models AP-5000, AP-10ML) •... -
Page 22: Limited Warranty
Corning of any such defect. Corning is not liable for any incidental or consequential damages, commercial loss, or any other damages from the use of this product. - Page 23 This warranty gives you specific legal rights. You may have other rights which vary from state to state. No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty.
- Page 24 55 (11) 3089-7400 t 65 6572-9740 Mexico Taiwan t (52-81) 8158-8400 t 886 2-2716-0338 For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners. © 2022 Corning Incorporated. All rights reserved. 1/22 CLS-A-EQ-015DOC REV1...
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