Subscribe to Our Youtube Channel
Related Manuals for WPI PV850
Summary of Contents for WPI PV850
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL PV850 Microinjector for Use with External Pressure Source (Pressure source not included) Serial No._____________________ www.wpiinc.com 113020...
-
Page 3: Table Of Contents
PV850 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 Features............................2 ts ............................2 Applications ..........................2 Notes and Warnings ......................... 2 Parts List ............................3 Unpacking ........................... 3 INSTRUMENT DESCRIPTION ......................4 Front Panel ..........................4 Rear Panel ........................... 5 Foot Switch .......................... - Page 4 World Precision Instruments...
-
Page 5: About This Manual
Fig. 1—MIRO-ePUMP Microinjector requires an external pressure source. INTRODUCTION Designed to simplify intracellular injection and a variety of other micro in jec tion tasks, the PV850 us s ar u r u at a r r s sur or n volumes range from picoliters to nanoliters. -
Page 6: Features
The PV850 off rs s arat r u at nsat on ( a nt on) an ejection pres sures with a pre ci sion timing circuit that switches from injection pressure to compensation pressure au to mat i cal ly. -
Page 7: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 27 of this manual. Please contact WPI Customer arts ar... -
Page 8: Instrument Description
Touch screen interface Adjustment Knob Injector Port Fig. 2—The major features of the PV850 are labeled in this diagram. Touch Screen Interface – The responsive panel allows for intuitive control. Touch t on ount r to r s t t t... -
Page 9: Rear Panel
TTL signal Fig. 3—The foot switch connection, power supply connection and power switch are located on the back panel of the PV850. Foot Switch Connection – Plug the connector from the foot switch into the connection port marked with the foot switch icon. Press the foot switch to initiate a... -
Page 10: Foot Switch
You can store and recall up to three of your most used protocols in the PV850 memory. If the box is empty, no protocol is loaded. Clearing Clogged Tip – Press Clear Tip to force a 500 ms burst of maximum pressure... -
Page 11: Setting Menu
Timed injections use the duration set on the main screen. Units – Select one of the checkboxes to set the display to either PSI or kPa. Save/Load Protocols – The PV850 can store your three favorite parameter sets ( roto o s) On t... -
Page 12: Setup
Fig. 7—The Foot Switch Connector is labeled “Rear Panel.” Do NOT plug it into the injection port on the front panel. NOTE: DO NOT connect the capillary kit hose to the PV850. Verify that the capillary kit hose is disconnected from the Injection Port. Then, power on the PV850. - Page 13 PV850 Fig. 8—(Left) The power up screen shows the software revision on your PV850. Fig. 9—As the self-test runs through the programmed diagnostic sequence, the display shows the results. Press the touchscreen to access the main screen. By default, the injection port is disabled.
- Page 14 803130 Y stereo splitter to the foot switch port on the rear of the PV850. Connect the CBL102 jack to one of the female ports on the Y stereo splitter. Plug the BNC cable into the appropriate TTL port.
-
Page 15: Operating Instructions
To change the dial adjustment speed, press the knob on the front of the PV850. The snail mode allows movements in 0.01 increments to the compensation pressure only, and only when it is below 3.0 PSI. Both conditions must be met. - Page 16 Gross Adjustment Fine Adjustment Fig. 18—Touch the parameter in the center column of the screen that you want to adjust and rotate the knob to modify the parameter. Press the knob to toggle between gross and fine tuning adjustments, indicated by the running man and the snail on the screen. Two seconds after a parameter is adjusted, it is saved into the memory.
-
Page 17: Saving A Protocol
PV850 Adjust the PV850 Settings s tt n s t on ( otto t orn r o t a n s r n) s a s t urr nt s tt n s for the PV850 ( anua or Fig. 20—(Left) The Setting area is located in the bottom right corner of the main screen. -
Page 18: Loading A Protocol
Fig. 23—The Settings menu lets you save a protocol or load a saved protocol. To bring up a saved protocol with the PV850 parameters, press the LOAD 1, LOAD2 or LOAD3 button. The unit loads the saved parameters of Protocol 1, Protocol 2 or Protocol 3, depending on which LOAD button you chose. -
Page 19: Techniques In Microinjection
When the micropipette is properly installed, click on the Settings area to access the Settings menu and press the Port OFF button to enable the Injection Port. TECHNIQUES IN MICROINJECTION The PV850 as an n tas s ro n t on o In this section, we will look at several important things to keep in mind when working with the PV850. -
Page 20: Manufacturing Micropipettes
(s an at on n r as s t θ in the LaPlace equation Manufacturing Micropipettes Pulling suitable micropipettes is one of the biggest obstacles to taking full advantage of the PV850 ar an st a an s ar r markedly dependent on the micropipette tip size. -
Page 21: Calibrating Volume By Measuring Droplet
Fig. 26—(Left) The volume of a droplet increases as the diameter increases. See “Appendix A: Droplet Volume” on page 23. Fig. 27—(Right) WPI’s MicroFil is helpful in backfilling glass pipette tips. The MicroFil’s tip is thin and very flexible. o o n ta s us u or on rt n ff... -
Page 22: Calibrating Rate Using A Known Volume
1 nl injection by counting the time between 1 ar s as t NOTE: The PV850 timing should not be adjusted to go below 10–15 ms as an absolute minimum, since it takes 6–10 ms for the pressure valves to respond. -
Page 23: Multibarrel Microinjection
The A drop of hot glue ts n u an o provides a secure, leak-free seal. allows use of a single PV850 to drive up to six micropipette barrels independently. Fig. 28—PolyFil multibarrel micropipette coupling kit Pipette pulled from... -
Page 24: Maintenance
MAINTENANCE The PV850 has been designed to yield reliable performance. However, some a orator on t ons u r o as ona r nt o t r ssur t rs this is necessary, return the instrument to the factory. If the unit displays a warning indicating that the pump needs service, please return it to the factory. -
Page 25: Troubleshooting
Injection Port disabled. After addressing the failure source, press the yellow banner to re-enable the port. On power up the PV850 performs a diagnostic self-test. If there is a failure during the self-test, an error code displays and the unit stops the sequence. It will not progress... -
Page 26: Specifications
SPECIFICATIONS s un t on or s to t o o n s at ons PRESSURE Time Interval 0.001 - 2.000 s Time Increment 0.001 s Injection Pressure Range, Controlled PS ( Injection Pressure Increment PS ( Injection Pressure, Uncontrolled PS ( Compensation Pressure Range, Controlled PS (... -
Page 27: Appendix A: Droplet Volume
PV850 APPENDIX A: DROPLET VOLUME Use the chart below to gauge the volume of a droplet and the table to determine the volume a micropipette can hold. 1 cm 215 µm 1 mL = 1.24 cm 10 nL = 267 µm 4.64 mm... -
Page 28: Bibliography
BIBLIOGRAPHY R.E. McCaman, D.G. McKenna and J.K. Ono, experimental parameters on hydrophobicity , A Pressure System for Intracellular and Extracellular Ejections of Picoliter Volumes , Capecchi, M.R., trans or at on ra n s ar by direct microinjection of DNA into cultured T.W. - Page 29 PV850 World Precision Instruments...
-
Page 30: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 31: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 32 China & Hong Kong WPI Shanghai Trading Co., Ltd. Rm 27A, Liangfeng Building, No 8, Dongfang Road, Pudong Free Trade Zone, Shanghai, PR China nasa s Brazil sa a Santos S o Pau o ra Internet www.wpiinc.com • www.wpi-europe.com • www.wpiinc.net • www.wpibrasil.com.br...
Need help?
Do you have a question about the PV850 and is the answer not in the manual?
Questions and answers