Related Manuals for WPI EVA-AC-03-03-12
Summary of Contents for WPI EVA-AC-03-03-12
- Page 1 INSTRUCTION MANUAL EVA-AC-03-03-12 SUMILON™ Companion Plate for Cell Culture Inserts for Use with EVOM™ Auto (24-Well) www.wpiinc.com 040325 040325...
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Page 3: Table Of Contents
EVA-AC-03-03-12 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 SETTING UP AUTO EXCHANGE PLATES WITH EVOM AUTO AND CELL CULTURE ™ INSERTS ............................. 2 USING WITH CORNING 24 INSERTS ................... 7 USING WITH MATTEK 24 INSERTS ....................11 COMPATIBLE INSERTS & RECOMMENDED VOLUMES ............15 Copyright ©... - Page 4 World Precision Instruments...
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Page 5: About This Manual
EVA-AC-03-03-12 ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. -
Page 6: Setting Up Auto Exchange Plates With Evom Auto And Cell Culture Inserts
SETTING UP AUTO EXCHANGE PLATES WITH EVOM AUTO AND CELL CULTURE INSERTS ™ To be able to use the EVOM Auto, 24-well Auto Exchange Plate, and cell culture ™ inserts, there are a few accessories that are needed. The 24M EVOM Auto Head, ™... - Page 7 EVA-AC-03-03-12 Fig. 2—24M EVOM Electrode Array Head and C24/M24 Plate Positioner inserted. ™ Fig. 3—24-well Auto Exchange Plate. Take note of the indent on the front right corner. World Precision Instruments...
- Page 8 Take note of the indent Fig. 4—Complete Assembly. The Indent should always be in this position to ensure correct plate orientation. Looking at the Auto Exchange Plate wells, as seen in Fig. 5, there is a larger primary well area with numerous kinds of tabs that will act as the adapter for different kinds of inserts.
- Page 9 EVA-AC-03-03-12 Front Left / Center Rear Right/ Basolateral electrode placement electrode placement Fig. 5—Close-up of 24-well Auto Exchange Plate. Fig. 6—Electrodes aligned over the Auto Exchange Plate. World Precision Instruments...
- Page 10 The following steps need to be followed to use 24-well cell culture inserts on the Auto Exchange Plate. In this guide, the Corning 24 and MatTek 24 inserts have been used as examples with details on how to set them up. Please refer to Fig. 7 for Corning 24 and MatTek 24 cell culture inserts.
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Page 11: Using With Corning 24 Inserts
EVA-AC-03-03-12 USING WITH CORNING 24 INSERTS For this section, the set up for Corning 24 inserts is shown. Carefully place a Corning insert into the larger well, as shown in Fig. 8. The orientation of the insert has no significance when using Corning 24-well inserts. - Page 12 Fig. 9—Electrodes are aligned with the Auto Exchange Plate with Corning 24 inserts. Fig. 10—Different angle of view of electrodes aligned with the Auto Exchange Plate with Corning 24 inserts. World Precision Instruments...
- Page 13 EVA-AC-03-03-12 While in the Expert window, click on measure position button in the well plate adjustments area. Now the electrodes should be inside the Corning insert and the Auto Exchange Plate. Use left and right adjustments as needed to properly...
- Page 14 Make sure to save your settings and return to the Main window. Fill the insert and the auto exchange plate well with 160mM KCl solution. You may use sterile media or buffer to run this test instead of KCl solution. We recommend liquid volumes of 1200µL/1.2mL in the basolateral well of the Auto Exchange Plate and 200µL/0.2mL for the Corning insert.
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Page 15: Using With Mattek 24 Inserts
EVA-AC-03-03-12 USING WITH MATTEK 24 INSERTS The MatTek 24 insert set up will follow a very similar principle as the Corning 24 insert. Take note of the three tabs on the upper rim of the MatTek insert. To successfully install the insert into the 24-well Auto Exchange Plate, have one of the three tabs pointed towards the back right of the well, where the opening that leads into the basolateral well. - Page 16 Fig. 13—MatTek 24 insert improperly placed. Select Expert from the Main menu to access the Expert window. Click on the travel position button in the well plate adjustments area and observe the position of the electrodes. The front left (center) electrode should be positioned near the center of the MatTek insert, and the rear right (basolateral) electrode should be in the basolateral well of the Auto Exchange Plate, outside of the edge of the MatTek insert.
- Page 17 EVA-AC-03-03-12 While you are on the Expert window, click on the measure position button in the well plate adjustments area. Now the electrodes should be inside the MatTek insert and the Auto Exchange Plate. Use left and right adjustments as needed,...
- Page 18 Save your settings and return to the Main window. Fill the insert and Auto Exchange Plate well with 160mM KCl solution. You may use sterile media or buffer to run this test instead of KCl solution. We recommend a basolateral liquid volume of 1750µL/1.75mL in the basolateral chamber of the Auto Exchange Plate, and 300µL/0.3 mL in the apical MatTek insert.
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Page 19: Compatible Inserts & Recommended Volumes
EVA-AC-03-03-12 COMPATIBLE INSERTS & RECOMMENDED VOLUMES Please follow the recommended liquid volumes mentioned in the table below to use 24-well cell culture inserts inside the Auto Exchange Plates and measure TEER with EVOM Auto. ™ Min. Apical Min. Basolateral Volumes Required... - Page 20 W OR LD P RE C I SI O N I NSTR U M E N TS...
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