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Summary of Contents for WPI Nanoliter 2000
- Page 1 Nanoliter 2000 Microprocessor controlled nanoliter injection INSTRUCTION MANUAL Serial No._____________________ 092512 World Precision Instruments...
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Page 3: Table Of Contents
Cleaning Recommendations................... 12 OPTIONAL ACCESSORIES ..................... 12 TROUBLESHOOTING ...................... 13 SPECIFICATIONS ....................... 13 APPENDIX: NANOLITER 2000/MICRO4 VOLUME SETTINGS ......14 Setting the Correct Volume on the Micro4 ............15 INDEX ..........................17 DECLARATION OF CONFORMITY ................18 WARRANTY ........................19 Claims and Returns .................... - Page 4 World Precision Instruments...
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Page 5: About This Manual
NOTES and TIPS contain helpful information. Reversing mount 180º will reduce manipulator-to-tip distance by 10cm. Universal Adapter for Nanoliter Injector Mounting Rod: 8.2Ø x 50mm Total volume in pipette: 1~3” = 4.9–17.4µL Fig. 1–Nanoliter 2000 setup. Micromanipulator is not included . World Precision Instruments... -
Page 6: Introduction
INTRODUCTION The Nanoliter 2000 microinjection system provides you with the capability to inject liquids in discrete aliquots ranging from 2.3nL to 69nL. The Nanoliter Injector utilizes a stainless steel plunger to create a mechanical displacement of fluid within a micropipette, thus pushing the contents out through the pipette tip. -
Page 7: Unpacking
Please read the section entitled “Claims and Returns” on page 19 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. -
Page 8: Instrument Description
Nanoliter Injector (shaft diameter of 8.2mm) is used for mounting the Nanoliter Injector to a micromanipulator. The Nanoliter 2000 is comprised of a controller (Fig. 3) and an injector handle (Fig. 4). Fig. 3 shows the right side of the controller where the DIP switches are located. -
Page 9: Controller Buttons
Clear Stop O-ring with Large Hole Collet Glass Fig. 4–Nanoliter 2000 (exploded view) shows the parts of the Nanoliter 2000 injector handle Controller Buttons Fill–The Fill button retracts the wire plunger by one aliquot volume when the button is depressed. A beep sounds at the extreme fill position. DIP switch #5 (Fig. -
Page 10: Plunger In "Home" Position
Plunger in “HOME” Position When you plug in the Nanoliter 2000, it assumes it is in the “home” position. When in this position, the tip of the wire plunger should be slightly recessed from the end of the collet. This prevents accidental damage to the plunger when it is not in use. -
Page 11: Operating Instructions
Nanoliter 2000 OPERATING INSTRUCTIONS The precise operation of the Nanoliter 2000 depends greatly on the use of tips prepared from the glass provided. Micropipettes pulled from capillaries with other dimensions may not work. CAUTION: Never attempt to use micropipettes pulled from glass containing a filament. -
Page 12: Micropipette Filling Techniques
Plastic Sleeve (spacer) “O” ring with large hole placed between “O” rings with indentation toward glass Collet (K3) Plunger Aluminum Barrel Glass Sealing “O” ring (small hole) Fig. 5–Standard configuration for installing the micropipette 3. Once positioned, tighten the collet securely. Give the micropipette a pull to confirm it is securely mounted. -
Page 13: Front Filling
2000. 4. Once the micropipette is installed, submerge the micropipette tip into the sample. 5. Press the Fill button on the Nanoliter 2000 controller to pull the sample into the tip of the micropipette. Injecting Injection aliquot volumes are determined by the position of the DIP switches, located on the right side of the Nanoliter 2000. -
Page 14: Setting The Aliquot Volume And Injection Speed
TIP: To set the DIP switches, use a ball point pen or a jeweler’s flat head screw driver. NOTE: DIP switch settings for the Nanoliter 2000 are completely different from the previous model, the Nanoliter Injector A203. Table 1: DIP Switch Settings... -
Page 15: Maintenance
After a period of time or heavy use, you may observe some leakage around the seals. To correct this, replace the O-rings. Replacement O-rings are included with your Nanoliter 2000. Additional O-rings (WPI #5340) may also be ordered. See “Securing the Micropipette to the Injector” on page 7. -
Page 16: Cleaning Recommendations
The injector can be cleaned by removing the collet, O-rings and spacers and wiping them with alcohol. CAUTION: Do NOT soak the Nanoliter 2000 parts in liquid. Do NOT autoclave the Nanoliter 2000. The control box may be cleaned by wiping it with a clean, damp cloth. -
Page 17: Troubleshooting
6 for instruction on resetting the home position. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. SPECIFICATIONS The Nanoliter 2000 conforms to the following specifications:... -
Page 18: Appendix: Nanoliter 2000/Micro4 Volume Settings
APPENDIX: NANOLITER 2000/MICRO4 VOLUME SETTINGS When using the Micro4 to control injections with the Nanoliter 2000, take care when entering the injection volume. The Nanoliter 2000 injector’s volume per step is based on the movement of the plunger wire inside a pulled glass pipette. -
Page 19: Setting The Correct Volume On The Micro4
(10.35nL), but the actual injection is still only 9.2nL. To avoid this error, enter only multiples of 2.3nL when calculating required volumes. Fig. 8–Micro4- an optional microprocessor-based controller, can provide an intelligent and easy-to-use interface for up to four Nanoliter 2000 injections. World Precision Instruments... - Page 20 World Precision Instruments...
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Page 21: Index
Nanoliter 2000 INDEX Symbols Micro4 Volume 14 4878 12 MicroFil 2, 7, 12 4879 12 micromanipulator 4 5340 12 micropipette 9 13142 12 300033 12 oocyte 2 500778 12 parts list 2 accessories 12 plunger 2, 11, 13 alcohol 12... -
Page 22: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 23: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 24 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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