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Summary of Contents for WPI UltraPath 2
- Page 1 SYSTEM GUIDE for visible and ultraviolet (UPUV-2) systems UltraPath 2 Multiple pathlength sample cell for absorbance spectroscopy with extended dynamic range Serial No._____________________ www.wpi-europe.com 180522...
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Page 2: Table Of Contents
How to fill the UltraPath 2 sample cell ................15 Acquiring Data with TIDASDAQ – a typical measurement cycle ........16 Refractive index sensitivity of the UltraPath 2 sample cell ..........18 - salinity matched reference solutions to avoid baseline offsets ........18 Measurement reliability .................... -
Page 3: General Warnings And Cautions
Warning: Dangerous voltages are present. No user serviceable parts inside unit. Instrument should be serviced by qualified service personnel only. Warning: The UltraPath 2 sampling system contains cleaning solutions. only qualified personnel should use these solutions. Please refer to the SDS Sheets for details on the solutions. -
Page 4: Introduction
10, 50 and 200 cm. Designed for the detection of low absorbing species in solution, UltraPath 2 is an ideal tool for any study requiring precise and highly sensitive spectroscopic determination of analytes, either in the lab or in the field. The instrument operates in the wavelength range of 220 to 720 nm (UPUV-2) and has an exceptional dynamic range allowing reliable absorbance measurements between 5μAU/cm to... -
Page 5: Instrument Description
0.2 mAU. The spectrometer includes an integrated deuterium/halogen light source which delivers light via the supplied fiber optic cables to the UltraPath 2 sample cell. A peristaltic pump (Peri-Star Pro) is utilized to evenly draw sample up into the Sample Injector and through the UltraPath 2 sample cell. -
Page 6: Ultrapath 2 Sample Cell
UltraPath 2 Sample Injector Kit. The Sample Injector (WPI #58006) is connected to the Liquid Input connector (7). Sample is drawn into the UltraPath 2 sample cell with the Sample Injector using vacuum suction of the Peri-Star Pro peristaltic pump. -
Page 7: Tidas S 300 Uv/Vis Spectrometer
TIDAS S 300 UV/VIS spectrometer module and this LED will be lit. 11. Fiber optic input (SMA type). The Fiber optic input connects to the light output of the UltraPath 2 sample cell (4). 12. Debug RS-232. Interface for system diagnostics (connectors supplied). For further instructions refer to TIDAS S 300 UV/VIS instruction manual. -
Page 8: Fiber Optic Cables
Fiber Optic Cables 18. Fiber optic cable — Light is coupled into the fiber optic input connector (1) of the UltraPath 2 sample cell from the TIDAS S 300 UV/VIS light output (22) with a 600 μm core fiber (WVLUXDUV-S-0600-SMA). -
Page 9: Cleaning Kit (Available Us Only)
Refer to the instruction manual for details. Cleaning Kit (Available US Only) 26. Waveguide Cleaning Kit. The waveguide cleaning kit contains three solutions specifically developed to keep the UltraPath 2 sample cell clean and ensure a high measurement accuracy and repeatability. LWCC Injection System 27. -
Page 10: Setting Up Ultrapath 2
10mm, 3.5ml * Used when you need to measure particulate absorption on glass fiber filter pads (see Setting up QFT2 Glass Fiber Filter section) – not used with the UltraPath 2 System setup. Opening the package Upon receipt of this system, make a thorough inspection of the contents and check for possible damage. - Page 11 Please read the section entitled “Claims and Returns” on the warranty page of this manual. Please call WPI Technical Support if any parts are missing. 11 | P a g e...
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Page 12: Assembling The Ultrapath 2 System
UltraPath 2 sample cell. Use a 600 μm core diameter fiber optic cable (19) to connect the light output (4) of the UltraPath 2 Fiber Optic Switch to the fiber optic input (11) of the TIDAS S 300 UV/VIS spectrometer. -
Page 13: Installing The Software
The 89372-2 LWCC Injections system together with the LOOP-5ML can be used in the setup to provide an efficient, continual flow for injecting a sample through the UltraPath 2. It minimizes contamination and the formation of tiny bubbles that interfere with spectroscopic data recording. -
Page 14: Setting Up Qft2 Glass Fiber Filter (Gf/F) & Cuvette Holder
Setting up QFT2 Glass Fiber Filter (GF/F) & cuvette holder The components required to measure particulate absorption on glass fiber filter pads are: TIDAS S 300 UV/VIS UV/VIS Spectrometer system (190-720 nm) including software, DH Lightsource. WVLUXUVIS-S-1000-100- UVIS Fiber (260-1200 nm) 1000 µm 100 cm SMA WVLUXUVIS-S-0600-100- UVIS Fiber (200-1200nm) 600 µm 100 cm... -
Page 15: Using Ultrapath 2
60 RPM or 12mL/min via the control panel (21). The sample is drawn into the waveguide by vacuum suction. • Select a pathlength (10, 50, 200 cm) at the fiber optic switch (4) of the UltraPath 2 sample cell. How to fill the UltraPath 2 sample cell •... -
Page 16: Acquiring Data With Tidasdaq - A Typical Measurement Cycle
Acquiring Data with TIDASDAQ – a typical measurement cycle After the light source is running for 15 minutes, the waveguide is filled with reference solution and the TIDASDAQ software started. Measurements can then be taken by the following steps. Define Directory, Filename and Comment of your dataset. Press the Settings icon to open the TIDASDAQ Parameter window. - Page 17 3. Press the Scan Start icon to start a continuous scan. 4. Start the pump and draw sample into the UltraPath 2 sample cell (See “How to fill the UltraPath 2 sample cell” for details). 5. Introduce an air bubble of approximately the length of the Injector tubing at the liquid input of the UltraPath 2 sample cell and draw the sample into the cell.
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Page 18: Refractive Index Sensitivity Of The Ultrapath 2 Sample Cell
Typical flow rates of the UltraPath 2 sample cell are 12 mL/ min (equal to 60 RPM with the Peri-Star Pro pump using size #14 tubing). Drawing liquid into the cell via the injector fills the sample cell. -
Page 19: Effective Pathlength And Linearity
A is absorbance, �� is the absorption coefficient, �� is concentration and �� is the optical pathlength of the sample cell. UltraPath 2’s three pathlengths (10, 50 and 200 cm) are manufactured with a tolerance of ± 2 mm and calibrated experimentally with a dye solution (Amaranth). -
Page 20: Using The Qft2 Cuvette Holder For Particulate Absorption Measurements
Using the QFT2 cuvette holder for particulate absorption measurements Background The rugged and portable QFT2 is specially designed for measuring absorption of particulate concentrated on Glass Fiber Filter (GF/F) Pads and for simple cuvette measurements in 10 mm cuvettes. Instead of collecting samples and transporting them to a laboratory, samples can be measured on-site. -
Page 21: Taking Particulate Absorption Measurements With A Gf/F Pad
*(λ) = uncorrected optical density of the collected sample on the GF/F pad = filtered volume = optical clearance area on the GF/F Pad Coefficients a and b are filter-type dependent (Whatman GF/F fi lters: a =0.392, b = 0.665). Taking particulate absorption measurements with a GF/F pad •... -
Page 22: Relevant Literature For Particulate Absorption Measurements
Relevant Literature for particulate absorption measurements A detailed description on measuring particulate absorption of fresh water and seawater with Glass Fiber Filters (GF/F) and determining the particulate absorption coefficient a (λ) concentrated on the filter has been published in the following papers: Mitchell, B. -
Page 23: Instrument Maintenance
It is recommended that the user confirm these after first receiving the instrument. The optional cleaning kit (WPI #501609) can be used with the UltraPath 2 system. It consists of three solutions numbered 1, 2, and 3. Organic contamination can be cleaned very efficiently using these solutions in sequence, as described below. - Page 24 Experiments have shown that three to five cleaning cycles were sufficient to clean the cell or organic contamination. In extreme cases (for example, CDOM left in the UltraPath 2 cell overnight), it was found that filling the waveguide with Solution 1 and letting it sit for several minutes (e.g., 10 minutes) and then subsequently flushing with Solutions 2 and 3 were...
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Page 25: Self-Test
Self-Test It is advisable to perform a self-test of the UltraPath 2 sample system before sampling. Reference light intensity scans of all three pathlength are supplied with each UltraPath 2 sampling system. These scans were taken with Millipore de-ionized water after the cells were rigorously cleaned. -
Page 26: Troubleshooting
UltraPath 2 cell. Ensure that the ferrules are installed properly; hand tighten the nuts. 2 . If the ferrules are deformed, exchange them. 3 . If the tubing end of the Injector is deformed and... - Page 27 S 300 UV/VIS contaminated UltraPath 2. Check if light exits at the 10, 50 or 200 detector cm output. This light will be dimmer than the light coupled into the sample cell.
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Page 28: Storage
QFT2 Where to go for more help If you have questions about any aspect of UltraPath 2, you are welcome to contact WPI at the offices specified on the back of the manual. Storage The UltraPath 2 system should be stored empty at temperatures between 10 ˚C to 60˚ C, preferably at room temperature and moderate humidity. -
Page 29: Accessories
Accessories Part Number Description WVLUXDUV-S-0600-100-SMA DUV Fiber (200-1200nm) 600um 100cm SMA WVLUXDUV-S-0200-100-SMA DUV Fiber (200-1200nm) 200um 100cm SMA 13395 SMA Bulkhead Feed Thru Connector, D-hole 58006 Sample Injector Assembly for UltaraPath2 58450 Kit, Adapter Syringe, LWCC 500320 Silicone tubing, 1m length, 1.6 mm I.D., 1.6 mm wall thickness CUV2012-1 Quartz Cuvette, Style C, 12.4x12.4x45mm, 10mm,... -
Page 30: Specifications
Specifications Dynamic range 0.002 m-1 – 230 m-1 (Absorption) 5 μAU cm-1- 1 AU cm-1 (Absorbance) Optical pathlengths Selectable: 10 cm, 50 cm and 200 cm Wavelength range 220 nm to 720 nm Inner diameter ≈ 1 mm Cell volume ≈... -
Page 31: References
References UltraPath 2 Richard L. Miller, Mathias Belz, Carlos Del Castillo, Rick Trzaska, “Determining CDOM Absorption Spectra in Diverse Coastal Environments Using a Multiple Pathlength, Liquid Core Waveguide System”, Continental Shelf Research, [in press] (July 2002) Vol. 22, Issue 9, p 1301-1310. - Page 32 November 13, 1998. P. K. Dasgupta, Z. Genfa, S. K. Poruthoor, S. Caldwell, S. Dong, and S.-Y. Liu, “High-Sensitivity Gas Sensors Based on Gas-Permeable Liquid Core Waveguides and Long-Path Absorbance Detection”, Anal. Chem., (1998), Vol. 70 pp. 4661-4669. Li. Song, S. Liu, V. Zhelyaskov, and M.A. El-Sayed, “Application of liquid waveguide to Raman spectroscopy in aqueous solution”, Appl.
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Page 33: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 34 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: +44 (0)1462 424700 • Fax: +44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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