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Leica BIOSYSTEMS APERIO GT 450 User Manual

Digital pathology slide scanner
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APERIO GT 450
DIGITAL PATHOLOGY SLIDE SCANNER
USER'S GUIDE
(NOT for use in China)

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  • Page 1 APERIO GT 450 DIGITAL PATHOLOGY SLIDE SCANNER USER'S GUIDE (NOT for use in China)
  • Page 2 Aperio GT 450 User's Guide MAN-0391, Revision G | December 2024 This manual applies to Aperio GT 450 Controller, Aperio GT 450 Console, and Aperio GT 450 SAM versions 1.4 and later. Original Instructions. Copyright Notice Copyright © 2019 - 2024 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are trademarks and registered trademarks of Leica Microsystems IR GmbH.

  • Page 3: Table Of Contents

    Help videos and guides Key features Continuous load workflow Scan magnification Automatic image quality check Manual Scanning Z-Stack scanning Extended Focus Image types supported Slide types supported Coverslips Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 4 Change the scan magnification for a rack of slides Z-Stack scanning Scan a rack of slides using z-stack scanning View z-stack images Extended Focus scanning Scan a rack of slides using Extended Focus Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 5 Six month maintenance Clean the objective and Koehler Materials needed Clean the stage slide tray Clean the carousel Clean the fan filter Clean the slide racks Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 6 Image transfer error – pending retry Aborted Aborted message appears on all slides Artifact detection messages from the optional Aperio iQC Software Module Symptoms and solutions Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 7 Touchscreen does not respond to touch Touchscreen is black Slides are broken inside the scanner Network connection lost White striping appears on scanned images Index Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 8: Notices

    DICOM converter. (on page 108) for clarity; revised Image transfer error – pending retry (on page 120) for clarity; revised Network connection lost (on page 124) for clarity. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 9: Cautions And Notes

    Cautions and Notes Serious Incidents Reporting — Any serious incident that has occurred in relation to the Aperio GT 450 shall be reported to the manufacturer and the competent authority of the member state in which the user is established.

  • Page 10: Intended Use

    As a system security measure, Leica Biosystems products capture and log external attempts to access system data. For more information, contact your Leica Biosystems representative. Training — This manual is not a substitute for the detailed operator training provided by Leica Biosystems or for other advanced instruction.

  • Page 11: Symbols

    Device is in compliance with UK Conformity Assessment Regulations 2016 and Low requirements. Voltage Electrical Equipment Regulations 1989 ISO 15223-1 - 5.4.4 Caution SO 7010 – W001 General warning Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 12 GB/T 26572- GB/T 26572-2011 2011. The outer circle indicates that the product can be recycled. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 13 California to cause Cancer and Reproductive Harm. For more information go to https://www.P65Warnings.ca.gov. Device is made in the USA of U.S. and foreign components. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 14: Introduction

    Introduction In this section: Resources Warnings Aperio GT 450 Compliance Specifications Installation Disposal of the Aperio GT 450 Scanner safety instructions Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 15 1 Introduction The Aperio GT 450 is a high performance, brightfield whole slide scanner that includes continuous loading with 450-slide capacity across 15 racks, priority rack scanning, automated image quality check and a scan speed of ~32 seconds at 40x scanning magnification for a 15 mm x 15 mm area.

  • Page 16: Resources

    Aperio GT 450 Specifications Provides detailed specifications for the scanner. Aperio GT 450 DICOM Upgrade Provides information on setting up the optional Aperio GT 450 1.3 DICOM Feature Guide Pack. Instructional videos Provides video instruction on performing various tasks. You can view the videos from the Help area on the scanner’s touchscreen interface.

  • Page 17: Instrument Warnings

    Component and part replacement warnings There are no user-replaceable parts or components in the Aperio GT 450 scanner. Replacement of parts or components within the Aperio GT 450 scanner must be performed by qualified Leica Biosystems Technical Services personnel using Leica Biosystems specified parts.

  • Page 18: Installation

    WARNING: Two people are required for lifting the scanner. Installation and setup of the scanner should only be performed by a trained Leica Biosystems Service representative. After installation, the Leica Biosystems Service representative will check the scanner for proper operation.

  • Page 19: Disposal Of The Aperio Gt 450

    If the package has visible damage from shipment, contact Leica Biosystems Customer Service for assistance. Disposal of the Aperio GT 450 The service life of the Aperio GT 450 is five years. After that time, cybersecurity patches and other technical support may not be provided.
  • Page 20 Moving – Use care if you must slide the scanner around the workbench. If you need to pick up the scanner to move it off the workbench, contact Leica Biosystems Technical Services. Moving the scanner may void the applicable product warranty—contact Leica Biosystems for advice.
  • Page 21 Unauthorized substitutions may result in fire, electric shock, or other hazards and may affect product compliance. The objective in the Aperio GT 450 scanner has been specifically designed for this device. Do not replace it with another type of objective.

  • Page 22: Aperio Gt 450 Overview

    Aperio GT 450 overview In this section: Theory of operation Scanner overview Touchscreen interface overview Help videos and guides Key features Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 23: Theory Of Operation

    This chapter provides an overview of the Aperio GT 450 Scanner. Theory of operation The Aperio GT 450 scanner contains an automated slide loader subsystem which is designed to process standard 1" × 3" (2.54 cm × 7.62 cm) microscope slides.

  • Page 24: Turn The Scanner On And Off

    Turn the scanner on and off The On/Off switch is located on the right side of the scanner near the back. On position = Off position = Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 25: Touchscreen Interface Overview

    The Home screen (Carousel view) is where you view rack and slide status. The buttons along the top enable you to access other areas, such as Help and Maintenance. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 26 63) Note: Rack settings appear when you Change the scan magnification for select a rack. a rack of slides (on page 47) Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 27: Help Videos And Guides

    Help videos and guides You can access training videos and the Aperio GT 450 Quick Reference Guide directly from the touchscreen interface. We recommend that you watch the training videos before operating the scanner for the first time.

  • Page 28: Key Features

    The scanner supports 1-inch x 3-inch (2.54-cm x 7.62-cm) glass slides (per ISO 8037/1). Coverslips Fully-cured coverslips are required. The Aperio GT 450 supports common industry coverslips used for 1 x 3 inch slides. Aperio GT 450 User's Guide, MAN-0391 Revision G...

  • Page 29: Slide Racks Supported

    We recommend using the Aperio Viewing Station for slide image viewing. The Aperio Viewing Station is optional and is not included in the Aperio GT 450 product. For more information, contact your Leica Biosystems representative. If you do not purchase the viewing station, we recommend using a calibrated monitor for optimal image viewing.

  • Page 30: Aperio Digital Slide Viewers

    2 Aperio GT 450 overview Aperio Digital Slide Viewers You can use the following viewers to view digital slides created on the Aperio GT 450 scanner: Aperio ImageScope (version 12.4 or higher) – this product comes pre-installed on viewing workstations (if purchased).

  • Page 31: Slide Preparation

    In this section: Slide preparation overview Slide preparation checklist Tissue preparation Staining Slide preparation guidelines Coverslips Labels Barcodes Slide quality control General guidelines for slide preparation Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 32: Slide Preparation Overview

    Slide preparation overview This section contains tips on best practices for slide preparation for your Aperio GT 450 scanner. To ensure the best possible quality and continued peak operation, it is crucial to ensure slides and scanner components are clean and free of obstructions.

  • Page 33: Slide Preparation Checklist

    Make sure that the variations of the staining process are controlled and eliminated to the greatest extent possible. Use appropriate morphological studies and controls as specified in the reagent manufacturer’s instructions. Avoid excessive stain residue, as this may affect scanner performance. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 34: Slide Preparation Guidelines

    Slides are fully cured (not “wet”). All slides have coverslips. Coverslips are required when using the Aperio GT 450 scanner. There is no mounting adhesive around the edges of a slide. This can cause it to stick or catch in the scanner stage area.

  • Page 35: Coverslips

    Wipe the coverslip clean of dust and fingerprints before loading the slide in the scanner. Labels The Aperio GT 450 supports common industry labels used for 1 x 3 inch (2.54 cm x 7.62 cm) slides. Improperly applied slide labels can cause slides to jam.

  • Page 36: Barcodes

    (covering tissue, barcodes), placement (label region), print quality and supported barcode type Coverslips Overhanging (skewed), lifting, multiple, bubbles, excessive mounting media (edges of slide, bottom), wet Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 37: General Guidelines For Slide Preparation

    Here are a few examples of poor sample preparation that may cause slide handling errors in a scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 38 Excessive mounting media around slide edges may lead to debris build up within the slide racks slots. This may cause slides to become affixed within the slide rack. Mounting media around the slide edges may also lead to slide load or unload errors. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 39 Ensure the following: The coverslip covers all the tissue and the label does not cover any tissue. The example below shows minimum distances from the side and bottom edges of a 26 mm x 76 mm slide. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 40: Scan Slides

    Check scan status Scan entire slide for whole rack Image quality control for histotechnicians and pathologists Scan statistics Manual Scanning Aperio iQC Software Module Notifications Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 41: Scanning Concepts

    This section provides basic concepts on the scanning workflow and the carousel rotation features. We recommend reviewing this section before using the scanner. Scan workflow The Aperio GT 450 is a continuous load scanner. You can continually load new racks and unload completed racks, as shown below: Aperio GT 450 User's Guide, MAN-0391 Revision G...

  • Page 42: Carousel Rotation

    Log in and log out of the scanner Your Lab Administrator sets up the scanner pass codes and timeout period for your lab using the Aperio GT 450 Aperio GT 450 SAM client application software. You need to log into the scanner to interact with the touchscreen interface. You do not need to log in to load and unload racks for scanning.

  • Page 43: Load Slides In The Rack

    CAUTION: Use caution when loading slides into a rack to prevent improper slide orientation or damaged slides. Never use damaged slides in the scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 44: Load Racks In The Carousel

    When the rack is fully inserted, there is a “click” sound, and the rack position on the Home screen turns light blue. Continue loading racks as needed or until you fill the front six rack slots. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 45 If the completed rack is in the back of the carousel, you can rotate it to the rack loading area. See Rotate a rack to the rack loading area (on page 55). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 46: Priority Scanning

    Tap the rack position. The Priority option appears when you select the rack position. Tap Priority. The priority icon and scan queue number appear on the rack position. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 47: 20X Magnification Scanning

    This section contains instructions for performing rack-level scans at 20x or 40x magnification. Your Aperio GT 450 scanner is initially set to scan all racks using 40x magnification. If you want this scanner to scan using 20x magnification by default, your administrator must turn on the DEFAULT TO 20X SCANNING option in SAM. For more information, see the Aperio GT 450 IT Manager and Lab Administrator Guide.
  • Page 48 The Scan at 40x option is selected, and 40x appears on the rack position. During scanning, the Rack View indicates the scanner is scanning this rack at 40x magnification. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 49: Z-Stack Scanning

    Scan a rack of slides using z-stack scanning For each scanner in Aperio GT 450 SAM, your Lab Administrator sets the default number of layers and the distance between layers (in microns) that are used for z-stack and extended focus scanning.​ You can adjust these settings on the scanner console for each rack of slides you scan.
  • Page 50 Lab Administrator previously set in SAM. (If your scanner has the optional Extended Focus feature installed, then the Extended Focus options also appear on this screen.) Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 51: View Z-Stack Images

    To view the 3-dimensional z-stack images, you must use a digital slide viewer that supports z-stack viewing, such as Aperio WebViewer (versions 1.2 and later), Aperio ImageScope, or a viewer of your choice that supports z-stack viewing. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 52: Extended Focus Scanning

    Scan a rack of slides using Extended Focus For each scanner in Aperio GT 450 SAM, your Lab Administrator sets the default number of layers and the distance between layers (in microns) that are used for z-stack and extended focus scanning.​ You can adjust these settings on the scanner console for each rack of slides you scan using the Extended Focus feature.
  • Page 53 The Z-STACK AND EXTENDED FOCUS SETTINGS screen appears with the default Number of layers and Layer separation (in microns) that your Lab Administrator previously set in Aperio GT 450 SAM. Follow these steps to adjust the Z-STACK AND EXTENDED FOCUS SETTINGS for the rack of slides you are...
  • Page 54 Under Output Type, select Extended Focus if it is not already selected. (Your Lab Administrator may have already set this option as a default in Aperio GT 450 SAM.) Note that Extended Focus image file names are appended with "_EF."...

  • Page 55: Rotate A Rack To The Rack Loading Area

    After the rack is rotated to the front, you can remove the rack. (The status light in the loading area blinks to indicate the location of the rotated rack). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 56: Unload Racks From The Carousel

    If the rack is in the loading area, carefully remove the rack from the rack slot. If the rack is not in the loading area, you can rotate it to the front: Tap the rack position. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 57: Check Scan Status

    CAUTION:  If you must remove a rack before all slides in the rack are scanned, note the rack and slide status. After removal, the rack’s scan status is no longer available. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 58: Check Rack Status

    If there is an error on at least one slide in the rack that is currently scanning, the rack position pulses blue and orange. Rack warnings (on page 61). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 59: View Slide Status For A Rack

    The slide scanned successfully, passed image QC, and was transferred to the specific image location. The slide has a scan warning. See Rack warnings (on page 61). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 60: View Macro Images Of Scanned Slides

    Racks in the list appear in the scan order. The scan status is indicated for each rack. In the example, rack 3 is set as priority. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 61: Rack Warnings

    Take note of the error code and message. For information and steps to resolve the rack error, go to Rack warnings and solutions (on page 115). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 62 An error occurred when transferring an image to the designated storage location A barcode is missing or invalid. (This issue only occurs if the Aperio GT 450 SAM is configured to require barcodes.) In the example below, slide 7 in the selected rack has a warning. ...

  • Page 63: Scan Entire Slide For Whole Rack

    Tap Settings , and then tap Scan Entire Slide. The Scan Entire Slide option is selected, and the Settings icon appears on the rack position: Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 64: Image Quality Control For Histotechnicians And Pathologists

    (Tap to navigate through the slides in the rack. You can view slide images only after the scanner has completed scanning the slide.) Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 65 If your scanner has the optional Manual Scan feature, tap Manual Scan to adjust the scan area. For more details, Manual Scanning (on page 67) Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 66: Scan Statistics

    Scan statistics The Aperio GT 450 provides scan statistics, such as the number of slides or racks scanned per day, per week, etc. Tap Stats to view statistics for the Last 7 Days, Last 12 Months, or Lifetime by Year.

  • Page 67: Manual Scanning

    Use Manual Scan to resolve an image quality issue If the scanner indicates a slide image has a quality issue, follow these steps to manually re-scan the slide: Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 68 Any slides with scan issues are colored orange, as shown below. Tap the Slide View icon. The macro image for the first slide in the rack appears. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 69 Tap the left or right navigation arrow (shown above) to display the problem slide, and then tap Manual Scan. The Manual Scan settings for the image open in the scanner console. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 70 Zoom to the scan area Tap the Focus View on Scan Area button to bring the scan area into view. Tap the button again to return to thefull view. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 71 When you are satisfied with your settings, tap Submit. The rack is added to the scan queue, with the manual scan settings applied to designated slide. You can prioritize the rack in the scan queue by following the instructions in Priority scanning (on page Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 72: Aperio Iqc Software Module Notifications

    Your IT Administrator sets up the communication between your Aperio GT 450 scanner and the Aperio iQC Software Module. For more details, see the Aperio GT 450 IT Manager and Lab Administrator Guide and your Aperio iQC Software Module IT Administrator's Guide.

  • Page 73: Maintenance

    Maintenance This chapter contains a maintenance schedule and procedures for maintaining your Aperio GT 450 scanner. Call Leica Biosystems Technical Services if you cannot perform a maintenance routine or if you discover a problem with your scanner. Before performing maintenance, we recommend you watch the maintenance videos available on the touchscreen. See Help videos and guides (on page 27).

  • Page 74: Maintenance Schedule

    Biosystems Technical Services. Serial number and firmware version You need the serial number and current software version if you call Leica Biosystems Technical Support. Tap Maintenance on the touchscreen interface to view the serial number, firmware version, and other system information.

  • Page 75: Shut Down The Scanner

    Complete these steps to access the inside of the scanner for maintenance or troubleshooting. If you are performing maintenance on the scanner, you should first remove any racks from the carousel. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 76 Grasp the cover using the handhold indentations: Slide the cover out until it reaches the point shown below. Rotate the VPU latch 180 degrees, as shown below. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 77: Recommended Daily Maintenance

    Before restarting the scanner, ensure the following: All racks are unloaded from the carousel. There are no slides scanning. The system does not have any errors displayed. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 78: Restart The Scanner

    Before restarting the scanner, ensure the following: All racks are unloaded from the carousel. There are no slides scanning. The system does not have any errors displayed. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 79: Inspect The Stage Slide Tray

    Ensure there are no slides scanning and the touchscreen interface does not display any errors. Open the scanner interior. For instructions, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 80 Wipe the slide tray again from back to front using a microfiber cloth. If necessary, use a gentle solvent such as denatured alcohol to remove any remaining residue. (To order a cleaning kit for this purpose, contact your Leica Biosystems representative. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 81: Inspect The Pusher/Puller

    If the pusher contact points contain debris, wipe them using a plastic scraper or edge of a blank glass slide, removing any mounting, debris, or high points. Use a lint-free cloth or alcohol wipes to clean the surfaces. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 82: Six Month Maintenance

    Do not drag anything (including lens paper) across the lens surface using a lot of pressure. This may damage the lens. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 83: Materials Needed

    Open the scanner cover. Rotate the VPU latch. Pivot out the VPU. For detailed steps, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 84 Manually position the slide stage so that you have clear access to the objective, as shown below. CAUTION: The objective may move when you're cleaning it. Wear gloves when cleaning the objective. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 85: Clean The Stage Slide Tray

    Restart the scanner after interior maintenance (on page 91). Clean the stage slide tray Frequency: at least every six months. To clean the stage slide tray: Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 86 Open the scanner cover and access the interior components (on page 75). CAUTION: The stage slide tray is not removable. Do not attempt to remove it. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 87: Clean The Carousel

    If the scanner is on, shut down the scanner by following the steps in Shut down the scanner (on page 75). Remove all slide racks from the carousel. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 88: Clean The Fan Filter

    The fan filter is located on the back of the scanner. You may need to pivot the scanner to access the fan filter. Frequency: at least every six months. To clean the fan filter: Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 89 Dry the filter thoroughly with a clean cloth or a dryer. CAUTION: Ensure the fan filter is completely dry before inserting it in the scanner. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 90: Clean The Slide Racks

    Frequency: at least every six months. To clean the scanner cover: Wipe the outer cover of the scanner with a damp cloth. Immediately dry the cover using a dry cloth. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 91: Clean The Touchscreen

    Follow these steps to restart the scanner after you have accessed the scanner interior. Pivot the VPU into the closed position. Secure the VPU in place by rotating the VPU latch 180 degrees. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 92: Transporting Or Moving The Aperio Gt 450

    Turn on the scanner. Transporting or moving the Aperio GT 450 If you need to relocate the scanner, contact Leica Biosystems Technical Services. Be aware that moving the scanner yourself may void the hardware warranty. Long term storage If you are not going to use the scanner for a considerable amount of time, shut it down and unplug it.

  • Page 93: Scanner Maintenance Log

    Clean slide racks Clean fan filter Clean touchscreen Clean scanner cover Yearly Maintenance Schedule annual maintenance visit *Perform visual inspection daily; clean if you observe debris. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 94: Troubleshooting

    Troubleshooting This chapter contains information and instructions to help you resolve troubleshooting issues with your scanner. For issues with the Aperio GT 450 Scanner Administration Manager, see the Aperio GT 450 IT Manager and Lab Administrator's Guide. WARNING: Do not attempt to perform troubleshooting procedures not shown in this chapter. For additional troubleshooting assistance, contact Leica Biosystems Technical Services.

  • Page 95: Personal Protective Equipment

    (on page 115) for steps to resolve slides in the rack. the specific error. The scanner can continue scanning other racks or slides. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 96: Perform A Safe Restart After An Error

    Follow these steps to safely restart the scanner after an error: Open the cover and access the interior: Open the scanner cover. Rotate the VPU latch. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 97 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. If the pusher is extended, return the pusher to the safe (retracted) position. Align the pusher teeth with the slide stage grooves. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 98 Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. Close the scanner cover. Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 99: Error Codes And Solutions

    Cause: An unexpected event occurred in the system that prevents it from continuing operation. Follow these steps: Open the cover and access the interior: Open the scanner cover. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 100 Open the scanner cover and access the interior components (on page 75). Take photos of any obstructions. Leica Biosystems Technical Services may request the photos if you need additional assistance after completing the steps below. Check if there is a slide on the stage or partially on the stage.
  • Page 101 Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 102 91). Tap Restart Scanner on the touchscreen interface, and wait for the scanner to complete the restart process. If the issue persists, call Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 103: 1001: Scanner Cannot Initialize

    Open the scanner cover. Rotate the VPU latch. Pivot out the VPU. For step details, see Open the scanner cover and access the interior components (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 104 If the pusher is extended, return the pusher to the safe (retracted) position. Align the pusher teeth with the slide stage grooves. Slide the stage to the rear of the scanner, as shown. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 105 For step details, see Restart the scanner after interior maintenance (on page 91). Shut down the scanner using the steps in Shut down the scanner (on page 75). Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 106: 1002: Carousel Cannot Rotate

    6 Troubleshooting Turn on the scanner and wait for the initialization process to complete. If the issue persists, call Leica Biosystems Technical Services. 1002: Carousel cannot rotate Cause: Something is blocking the light curtain. Follow these steps in order until the issue is resolved and the error message closes: Check the rack loading area and pinch points for any unexpected object.
  • Page 107 If there are no obstructions and the carousel still does not rotate, restart the scanner. See Perform a safe restart after an error (on page 96). If the issue persists, contact Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 108: 1003: Carousel Cannot Rotate. Carousel Pinch Point Obstructed

    The Lab Administrator should perform these steps: Ensure the LAN cables are connected at the scanner LAN port and at the Aperio GT 450 SAM server. Do not restart the scanner. If you restart the scanner, the scanned data is lost, and users have to rescan their slides.

  • Page 109: 2000: Slide Handling Error At Slide Stage, Rack, Or Pusher

    Open the scanner cover and access the interior components (on page 75). Take photos of the obstruction. Leica Biosystems Technical Services may request the photos if you need additional assistance after completing the steps below. Aperio GT 450 User's Guide, MAN-0391 Revision G...
  • Page 110 Check the slide stage, rack, and pusher area for an obstruction. If possible, carefully remove the slide that is causing the obstruction. WARNING:  Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. Check the slide for preparation issues, such as over-hanging coverslips and label issues.
  • Page 111 Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in one of the areas circled below. Avoid touching the LED and objective. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 112 91). Tap Restart Scanner on the touchscreen interface, and wait for the scanner to complete the restart process. If the issue persists, contact Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 113: 2001: Slide Handling Error At Rack Gripper, Lift, Or Carousel

    Open the scanner cover and access the interior components (on page 75). Take photos of the obstruction. WARNING:  Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 114 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. Check the rack gripper, lift, and carousel area for an obstruction. Close the scanner cover: Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 115: 9000: Scanner Cover Is Open

    CAUTION: If you need to remove a rack before all slides in the rack are scanned, first take note of the rack and slide status. After you remove a rack, the rack’s scan status is no longer available on the Home screen. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 116: 1005: Cannot Process Rack

    (on page 96). If the issue persists, remove the rack and contact Leica Biosystems Technical Services. 1006: Cannot process one or more slides in rack Cause: There is an issue with one or more slides in the rack.

  • Page 117: Slide Errors And Solutions

    If there is an issue scanning a slide, one of the following error messages appears in Slide View. Follow the steps in order until the issue is resolved. If you follow the steps and the issue persists, contact Leica Biosystems Technical Services.

  • Page 118: No Barcode

    Your Lab Administrator determines whether the scanner requires the presence of a barcode to complete a scan. Your Lab Administrator or Leica Biosystems representative sets this configuration in Aperio GT 450 SAM. If your site requires the use of barcodes, follow these steps in order until the issue is resolved: Check the slides and rack are inserted correctly: Slide label facing out and up.

  • Page 119: No Macro Focus

    Labels are not overhanging or on wrong side. There is only one label applied to the slide. Slide is clean. If every slide has the same error, or if the issue persists after following the previous steps, call Leica Biosystems Technical Services. Image quality Cause: The scanner’s Auto-Image QC feature detected an image quality issue.

  • Page 120: Image Transfer Error - Pending Retry

    Clean the objective and Koehler (on page 82). If the issue persists after following the previous steps, call Leica Biosystems Technical Services. Image transfer error – pending retry Cause: The scanner cannot transfer the scanned image to the image storage location.

  • Page 121: Aborted

    Clean the slide. Insert the slide into a different rack and scan it again. If the issue persists after following the previous steps, call Leica Biosystems Technical Services. Aborted message appears on all slides Follow these steps in order until the issue is resolved: Remove any completed slide racks from the carousel.
  • Page 122 If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components. Close the scanner cover: Pivot the VPU into place. Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

  • Page 123: Artifact Detection Messages From The Optional Aperio Iqc Software Module

    When touchscreen is dark, turn the scanner off using the On/Off switch. Turn the scanner back on using the On/Off switch. Allow the scanner to scan any remaining racks. If the issue persists, call Leica Biosystems Technical Services. Artifact detection messages from the optional Aperio iQC Software Module If you are using the optional Aperio iQC Software Module, messages preceeded by "iQC"...
  • Page 124 Leica Biosystems Technical Services may request photos when providing assistance. Network connection lost The Aperio GT 450 must be connected to the Aperio GT 450 Scanner Administration Manager (Aperio GT 450 SAM) through your Local Area Network to function. If that connection is lost, you see: Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright ©...
  • Page 125 Contact your IT Administrator for assistance. The IT Administrator should do the following: Ensure the LAN cables are connected to the scanner LAN port and to theAperio GT 450 SAM server. In the area provided on the scanner's touchscreen interface, enter the IP address of the Aperio GT 450 SAM server.
  • Page 126 Your Lab Administrator configures the Default Calibration Point feature in the Aperio GT 450 SAM. See the Aperio GT 450 IT Manager and Lab Administrator's Guide for details.
  • Page 127 Koehler insert slides clean objective installation continuous load instrument warnings Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 128 Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 129 Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.
  • Page 130 25, See also touchscreen close open warnings rack 61, 115 whole slide scan See scan entire slide workflow, scan Z-stack scanning 28, 49 Aperio GT 450 User's Guide, MAN-0391 Revision G Copyright © 2024 Leica Biosystems Imaging, Inc.

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