Leica BIOSYSTEMS HistoCore SPECTRA CV Instructions For Use Manual
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Leica BIOSYSTEMS HistoCore SPECTRA CV Instructions For Use Manual

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HistoCore SPECTRA CV
Coverslipper
Instructions for Use
English
Order No.: 14 0514 80101 - Revision L
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.2, Revision L - 02.2022

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Summary of Contents for Leica BIOSYSTEMS HistoCore SPECTRA CV

  • Page 1 HistoCore SPECTRA CV Coverslipper Instructions for Use English Order No.: 14 0514 80101 - Revision L Always keep this manual with the instrument. Read carefully before working with the instrument. Version 2.2, Revision L - 02.2022...
  • Page 3 – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the nameplate on the back of the instrument.

  • Page 4: Table Of Contents

    Table of contents Important Notes ......................7 Symbols and their meanings ....................... 7 Instrument type ........................11 User group ..........................11 Intended purpose ........................12 Copyright – Instrument software ....................12 Safety ........................13 Safety notes ..........................13 Hazard warnings ........................14 Safety features on the instrument ....................17 Instrument Components and Specifications ..............
  • Page 5 7.2.8 Filling and changing the needle cleaning container ..............110 7.2.9 Removing the complete unit of the needle cleaning container ...........111 7.2.10 Cleaning the Pick&Place module ....................114 7.2.11 Exchange suction cups ......................114 7.2.12 Cleaning the waste tray ......................115 7.2.13 Cleaning the reagent vessels ....................116 HistoCore SPECTRA CV...
  • Page 6 Table of contents 7.2.14 Rack and handle ........................116 7.2.15 C hanging the active carbon filter ....................117 7.2.16 Cleaning reagent vessels in the load drawer ................118 Preparing the hose system for priming and cleaning ..............118 7.3.1 Quick Prime ..........................121 7.3.2 Extended Prime ........................122 7.3.3 Cleaning the hose system......................123 7.3.4 Recommissioning after transport or storage ................127 Recommended cleaning and maintenance intervals ..............127 7.4.1 Daily cleaning and maintenance....................128...

  • Page 7: Important Notes

    Symbol: Title of the symbol: Check display for messages Description: Messages in the display have to be read by the user. Symbol: Title of the symbol: Manufacturer Description: Indicates the manufacturer of the medical product. HistoCore SPECTRA CV...
  • Page 8 Important Notes Symbol: Title of the symbol: Date of manufacture Description: Indicates the date when the medical device was manufactured. Symbol: Title of the symbol: CE Label Description: The CE marking is the manufacturer's declaration that the medical product meets the requirements of the applicable EC directives and regulations.
  • Page 9 Symbol: Title of the symbol: Observe the laser beam warning and Instructions for Use Description: The product uses a class 1 laser source. The safety notes for handling lasers and the Instructions for Use must be observed. HistoCore SPECTRA CV...
  • Page 10 Important Notes Symbol: Title of the symbol: IPPC symbol Description: The IPPC symbol includes: • IPPC symbol • Country code in accordance with ISO 3166, e.g. DE for Germany • Regional identifier, e.g. HE for Hesse • Registration number, unique number beginning with 49 • Treatment method, e.g. HT (heat treatment) Symbol: Title of the symbol: Country of Origin...

  • Page 11: Instrument Type

    User group • The HistoCore SPECTRA CV must only be operated by authorized personnel comprehensively trained in using lab reagents and their application in histology. • All laboratory personnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it.

  • Page 12: Intended Purpose

    Copyright – Instrument software The software installed and used on the HistoCore SPECTRA CV is subject to the following license agreements: 1. GNU General Public License Version 2.0, 3.0 2.

  • Page 13: Safety

    This instrument has been built and tested in accordance with the safety requirements for electrical equipment for measurement, control, and laboratory use. To maintain this condition and ensure safe operation, the user must observe all notes and warnings contained in these Instructions for Use. HistoCore SPECTRA CV...

  • Page 14: Hazard Warnings

    • ONLY technicians trained and permitted by Leica can connect a USB input device (mouse/keyboard, etc.) for service and application diagnostics only. In the interest of specimen safety, the HistoCore SPECTRA CV indicates when it is necessary for the user to intervene using on-screen messages and audible signals. Therefore, the HistoCore SPECTRA CV robotic coverslipper requires that the user is within hearing distance during operation.
  • Page 15 • The user is responsible for monitoring the expiration date of other reagents used with the HistoCore SPECTRA CV (e. g. xylene in the reagent vessels and the needle cleaning containers). Expired reagents must be replaced immediately and disposed of. When disposing of consumed, expired or used-up reagents, the applicable local regulations and the waste disposal regulations of the company/institution in which the instrument is being operated have to be observed.
  • Page 16 Safety Warnings – Operating the instrument Warning • The instrument may be operated by trained laboratory personnel only. It must only be operated for the purpose of its designated use and according to the instructions contained in these Instructions for Use. Antistatic protective clothing made from natural fibers (e.g. cotton) should be worn when working with the instrument.

  • Page 17: Safety Features On The Instrument

    • Opening the hood when one or more coverslipping process(es) is/are active causes delays in the respective processing steps since no transport movements take place for this time frame. • To ensure smooth operation of the instrument software, the instrument must be restarted by the user at least every 3 days. HistoCore SPECTRA CV...

  • Page 18: Instrument Components And Specifications

    Standard delivery – packing list Designation Order No. HistoCore SPECTRA CV basic instrument (local power cord included) 14 0514 54200 Rack for 30 slides (3 pcs. per package) 14 0512 52473 Handle for rack for 30 slides (yellow, 3 pcs. per package) 14 0512 52476 Handle for rack for 30 slides (light blue, 3 pcs.

  • Page 19: Technical Data

    2nd parameter = No protection against water Ambient conditions: Operation: Temperature: +18 °C to +30 °C Relative humidity: 20 % to 80 %, non-condensing Operating elevation: Up to a max. of 2000 m above sea level Storage: Temperature: +5 °C to +50 °C Relative humidity: 10 % to 85 %, non-condensing Transport: Temperature: -29 °C to +50 °C Relative humidity: 10 % to 85 %, non-condensing HistoCore SPECTRA CV...
  • Page 20 Dimensions Hood closed: 2044 x 785 x 615 mm and weights (length x depth x height): Hood opened: 2044 x 785 x 1060 mm (HistoCore SPECTRA CV and HistoCore SPECTRA ST): Weight, empty (without 280 kg reagents and accessories): Weight, filled (with reagents...

  • Page 21: General Overview - Front View

    Left coverslip line 10 Operating switch Right coverslip line 11 Screen with user interface Load drawer 12 Fuses Unload drawer 13 Pick&Place module Service access 14 Waste tray USB slot 15 Coverglass cartridge Main switch 16 Bottle sledge HistoCore SPECTRA CV...

  • Page 22: General Overview - Rear View

    Instrument Components and Specifications General overview - rear view Fig. 2 Network connection Access to activated carbon filter Power supply Height-adjustable instrument feet Exhaust air connection Version 2.2, Revision L...

  • Page 23: General Overview - Inside View

    13 Red bar Coverglass cartridge 14 Shifter and shifter tongue Needle 15 Needle cleaning container Bottle sledge 16 Coverslipping position of the slide Mounting medium bottle 17 Needle holder Mounting medium bottle 18 Elevator with locking mechanism HistoCore SPECTRA CV...

  • Page 24: Installation And Instrument Setup

    Installation and Instrument Setup Installation and Instrument Setup Installation site requirements Note • Installation, adjustment and level alignment of the instrument are carried out as part of the instrument installation by a service technician certified by Leica only. • The level orientation is done using a level and by adjusting the height-adjustable instrument feet (→ Fig. 2-5).

  • Page 25: Electrical Connection

    Main switch (→ Fig. 1-8). Fig. 4 4. After a short period of time, the Operating switch lights up orange. Once the software has finished starting up, the switch lights up red (→ Fig. 5-1) and the instrument is in standby mode. 5. The Operating switch can then be operated. Fig. 5 HistoCore SPECTRA CV...

  • Page 26: Internal Battery

    The UPS must be designed for operating voltage at the installation location. The connection is carried out by connecting the HistoCore SPECTRA CV power cable to the UPS power output socket. The UPS is connected to the power socket in the lab.

  • Page 27: Exhaust Air Connection

    2. For a simplified fitting of the inserts, the unload drawer can be further opened. For this purpose, raise the red retaining lever (→ Fig. 8-3) that moves the unload drawer completely out of the instrument and carefully fold it downwards. 3. Insert the three inserts (→ Fig. 8-1) into the compartments (→ Fig. 8-2) in the unload drawer. HistoCore SPECTRA CV...

  • Page 28: Inserting The Waste Tray

    Installation and Instrument Setup Fig. 8 4. Finally, lift the unload drawer and slide it back in the instrument. 4.4.2 Inserting the waste tray » Remove the waste tray (→ Fig. 9-1) from the packaging and insert it into the instrument at the recess (→ Fig. 9-2) provided.

  • Page 29: Filling And Inserting The Needle Cleaning Container

    6. Following this, plug the needle cleaning container in the slot and press downwards until it clicks into place. 7. Remove the needle from the parking location and plug it back into the holder (→ Fig. 87). Fig. 10 HistoCore SPECTRA CV...

  • Page 30: Switching On And Shutting Down The Instrument

    Switching on and shutting down the instrument Warning The instrument must be connected to a grounded power socket. For additional electrical fuse protection, connecting the HistoCore SPECTRA CV to a socket with a residual current circuit breaker (RCCB) is recommended. Note •...
  • Page 31 Furthermore, the level of the needle cleaning containers is checked during operation approximately every 4 hours. 6. The Operating switch is illuminated in green whenever the instrument is ready to start. 7. After completing the initialization phase, the Main menu (→ Fig. 12) appears on the screen. Fig. 12 HistoCore SPECTRA CV...

  • Page 32: Refilling Consumables

    • A check and a scan of the consumables is always performed after the hood has been closed. Note • If fewer than 300 slides are coverslipped per day on an ongoing basis, the HistoCore SPECTRA CV can also be operated with just a single coverslip line. In this configuration, do not fill any consumables (mounting medium, coverglass) in the unused coverslip line.

  • Page 33: Inserting A Coverglass Cartridge

    (→ P. 70 – 5.9.4 Properties of the coverglass) are available for the HistoCore SPECTRA CV. The coverglasses are only available packaged in magazines. The magazines are automatically read by the instrument when inserted and the data is sent to the consumables management system (CMS) (e.g. number and size).

  • Page 34: Inserting The Mounting Medium Bottle And Prime Bottle

    Installation and Instrument Setup Fig. 14 4.6.2 Inserting the mounting medium bottle and prime bottle 1. Open the hood. 2. Grasp the bottle sledge on the handle (→ Fig. 16-1) and move it all the way forward. Inserting the prime bottle » Insert the prime bottle (→ Fig. 16-2) into the position provided for it (→ Fig. 3-11)
  • Page 35 • Before the initial insertion of the cannulas, check whether the caps on the ends of the cannulas have been removed. Fig. 15 2. Insert the mounting medium bottle into the recess and make sure that the RFID chip (→ Fig. 15-4) is seated in the notch of the recess (→ Fig. 16-5). HistoCore SPECTRA CV...
  • Page 36 Installation and Instrument Setup Fig. 16 3. Carefully insert the cannula (→ Fig. 16-4) with the label 1 into the opening of the mounting medium bottle until it clicks into place. This process involves puncturing the white protective membrane. Warning Puncturing the white protective membrane briefly requires an increase in force. Therefore, proceed carefully to avoid slipping from your hand. 4.

  • Page 37: Prepare The Reagent Vessel, Fill It And Insert It Into The Load Drawer

    • Make sure that the reagent vessel handle is not bent. During the removal of the reagent vessel, if the handle is bent out of shape, it may detach from the reagent vessel and the reagent could be spilled. Warning The safety notes for reagent handling must be observed! HistoCore SPECTRA CV...
  • Page 38 Installation and Instrument Setup Correct fill level of the reagent vessels Note • When filling the reagent vessels, observe the fill level marks inside the reagent vessels. • Use the label covers contained in the (→ P. 18 – 3.1 Standard delivery – packing list) to label the reagent vessels in the load drawers. • The label covers with the letter printed on them (→ Fig. 18-2) indicate to the user that the...

  • Page 39: Operation

    Operation Operation User interface – overview The HistoCore SPECTRA CV is programmed and operated using a color touchscreen. If there is no processing in progress, the screen displays the following main window (→ Fig. 19) after switching on. Fig. 19 1 Status bar...

  • Page 40: Grayed Out Function Keys

    Operation 5.1.1 Grayed out function keys Note No changes can be carried out in the settings during processing or while there are racks in the instrument (load drawer, unload drawer, oven, coverslip line). Corresponding function keys are grayed out and therefore deactivated. However, at all times it is possible to: •...

  • Page 41: Elements Of The Status Display

    11 The operation of the instrument in Supervisor Mode is displayed by this symbol. This mode provides additional operation and adjustment options for trained staff. Access to this mode is password-protected. 12 This User symbol displays that the instrument is in user mode, which permits a simplified instrument operation without password. HistoCore SPECTRA CV...

  • Page 42: Process Status Display

    Operation Process status display • In the Process status display (→ Fig. 19-2), all racks in the process (→ Fig. 21-2) are displayed in color of the respective rack handle. • The status bar (→ Fig. 21-1) for the main window lists the parameter sets that can currently be started with the number and color assigned to the rack handles.

  • Page 43: Consumables Management System (Cms)

    Consumables Management System (CMS) Note To call up this menu, press the Module Status menu (→ Fig. 19-8) button. The HistoCore SPECTRA CV has a Consumables Management System (CMS) that automatically monitors various levels and notifies the user of the following details: • Mounting medium designation (→ Fig. 22-1) • Remaining number of slides (→ Fig. 22-2) •...

  • Page 44: Displaying The Drawers

    • If there is a rack transported by the instrument in the load or unload drawer, • If a rack is to be transferred from the HistoCore SPECTRA ST to the HistoCore SPECTRA CV, • If a rack is being turned in the rotator •...

  • Page 45: Main Menu Overview

    Operation Warning For manual loading of the HistoCore SPECTRA CV in workstation mode, the user is responsible to pay attention to racks being transferred from the HistoCore SPECTRA ST to the HistoCore SPECTRA CV at the same time. The end times of the HistoCore SPECTRA ST have to be taken into account before loading manually since otherwise there can be delays in the HistoCore SPECTRA ST that can affect the staining result.

  • Page 46: Entry Keyboard

    Operation 5.6.1 Entry keyboard Note A keyboard appears (→ Fig. 24) for required entries (creating or entering a password). It is operated using the touchscreen. Note that the keyboard display depends on the configured language. Fig. 24 Title bar Input field Delete the most recently entered character Confirmation Move cursor to left or right Space key Special character shift button (→ Fig. 25)
  • Page 47 2. Example: Holding the standard "a" button brings up other selection options (→ Fig. 26). 3. Select the required character from the new single-line keyboard by pressing it. Fig. 26 Note Number of characters for passwords: min. 4 to max. 16 characters. HistoCore SPECTRA CV...

  • Page 48: User Settings

    Operation User settings This menu can be used to configure the appropriate access level. A distinction is made between the following: • Standard user • Supervisor (password-protected) • Service technician (password-protected) Standard user: The standard user does not need a password and can use the completely configured instrument for all routine applications. It is not possible for this user group to modify programs and settings. Supervisor: The supervisor has the same access options as the standard user, but when the instrument is in idle mode, they can also create, edit and delete parameter sets, change settings and perform the instrument setup functions.
  • Page 49 To change the supervisor password, proceed as follows: 1. To change the password, press the Change Password button (→ Fig. 27-2) and enter the old password. 2. Then, enter the new password twice using the keyboard and confirm with OK. Note A password must have at least 4 characters and may have up to 16 characters. HistoCore SPECTRA CV...

  • Page 50: Basic Settings

    Operation Service technician: The service technician can access system files and carry out basic settings and tests. Note Access to this service area of the software is only possible for technicians trained by Leica and certified for this instrument type. Basic settings Settings (→ Fig. 29-1) menu is opened by pressing the gear symbol (→ Fig. 29). Basic instrument and software settings can be configured in this menu. • Touching one of the displayed symbols, e.g. Language (→ Fig. 29-2), opens the corresponding submenu.

  • Page 51: Language Settings

    • The time display can be changed from a 24-hour display to a 12-hour display (a.m. = morning/p.m. = afternoon) using the slider (→ Fig. 30-3). • Pushing the Save (→ Fig. 30-5) button saves the settings. • If you do not want to apply the settings, press the Back (→ Fig. 30-4) button to return to the previous menu. HistoCore SPECTRA CV...

  • Page 52: Date And Time

    Operation Fig. 30 5.8.3 Date and time Required access level: Standard user, supervisor The current date (→ Fig. 31-1) and local time (→ Fig. 31-2) can be configured in this menu by rotating the individual rollers. • Pushing the Save (→ Fig. 31-4) button saves the settings. • If you do not want to apply the settings, press the Back (→ Fig. 31-3) button to...

  • Page 53: Menu For Alarm Sounds - Error And Signal Sounds

    Warning After starting the instrument, an error sound is played. If this does not occur, the instrument may not be operated. This protects the specimens and the user. In this case, contact the responsible Leica service organization. HistoCore SPECTRA CV...
  • Page 54 Operation Fig. 32 Sound Type 1 - Note (→ Fig. 32-1) Signal sounds are issued if warning messages or notifications are displayed on the screen. You can select from a list of 6 sounds. To change the settings, press the Edit (→ Fig. 32-3) button. The Test (→ Fig. 32-5) button can be used to listen to the respective sound. The volume can be adjusted incrementally by turning the roller (0 to 9). Sound Type 2 - Error (→ Fig. 32-2) Warning The user must stay within earshot during the operation in order to react immediately during instrument...

  • Page 55: Oven Settings

    • The oven dries the cover slipped slides after completed processing for approx. 5 minutes. After the oven step has ended, the mounting medium is not completely dry. Treat the slides carefully during the removal from the rack to avoid a sliding of the coverglass. • The temperature setting of the oven (maximum 40 °C) and the dwell time of the finished coverslipped slides are both configured at the factory and cannot be changed by the user. • It can take up to 4 minutes for the oven to heat up! HistoCore SPECTRA CV...
  • Page 56 Operation Fig. 33 Shutting down the oven 1. To shut down the oven, press the Oven off (→ Fig. 33-2) button. 2. If the oven is shut down, this is indicated by the button (→ Fig. 33-2) being colored red/white. 3. Apply the shutdown by pressing the Save (→ Fig. 33-5) button.
  • Page 57 L The oven step at the end of processing can be disabled if necessary. In this case, the oven remains switched on and can be made available immediately again if necessary by reactivating the oven step. The heating phase is omitted. 1. To exclude the oven step, press the Exclude Oven Step (→ Fig. 33-3) button. HistoCore SPECTRA CV...
  • Page 58 Operation 2. If the oven step is excluded, this is indicated by the button (→ Fig. 33-3) being colored red with an X. Apply the configuration by pressing the Save (→ Fig. 33-5) button and note the subsequent information message (→ Fig. 36) and confirm it with Ok. Fig. 36 Warning Excluding the oven step leads to less effective drying and requires greater care when removing slides from the rack.

  • Page 59: Volume Calibration

    2. You can adjust the application quantity by turning the corresponding roller (→ Fig. 38-2) coverslip line (→ Fig. 38-1). Note Negative values (-1 through -5) reduce the application quantity for the selected coverslip line incrementally, while positive values (1 through 5) increase the application quantity incrementally. HistoCore SPECTRA CV...
  • Page 60 Operation To apply the configured values, press the Save (→ Fig. 38-3) button for the respective coverslip line, or L2. 4. Note the subsequent information message and acknowledge it with (→ Fig. 38-4). 5. Exit the menu by pressing the Back (→ Fig. 38-5) button. 6. To discard the settings, do not press the Save (→ Fig. 38-3) button, but instead press...

  • Page 61: Data Management

    Furthermore, the load and unload drawers must be closed. • The USB memory stick used must be formatted as FAT32. HistoCore SPECTRA CV...
  • Page 62 Operation User export (→ Fig. 39-1) User Export function is used to save information on a connected USB memory stick (→ Fig. 1-7): • A zip file with the event logs of the last 30 working days and CMS information in CSV format. • An encrypted lpkg file that contains all user-defined parameter sets and the consumables list. Note The encrypted lpkg file cannot be opened and viewed by the user. 1. After pressing the User Export button, the data is exported to the connected USB memory stick. 2.

  • Page 63: Event View

    Required access level: Standard user, supervisor A separate log file is created for each day that the instrument was switched on. This file can be called up by selecting the DailyRunLog file in the Event view menu (→ Fig. 40). In the Event View menu, an event log (→ Fig. 40) can be selected from a list of available logs (→ Fig. 40-1) and called up by pressing the Open (→ Fig. 40-2) button. Note The file name is supplemented by adding the respective creation date in ISO format, which makes organization easier. The file is created in the configured language. HistoCore SPECTRA CV...
  • Page 64 (→ Fig. 41-2) and the currently installed software version (→ Fig. 41-3) of HistoCore SPECTRA CV. • You can scroll up and down in the list and the log file using the arrow buttons (→ Fig. 41-4). • Pressing the left button allows you to scroll through the event viewer page by page. • Pressing the right button takes you to the beginning or the end of the event viewer.
  • Page 65 Operation Fig. 41 Time stamp Arrow buttons Serial number Event ID Installed Software version Message Color: Gray Indicates an event or information Color: Orange Indicates a warning message Color: Red Indicates an error message HistoCore SPECTRA CV...

  • Page 66: Parameter Settings

    Operation Parameter settings Note • The parameter set menu (→ Fig. 42) lets the user create new parameter sets, modify existing ones or assign a rack handle color to a parameter set. Creating or modifying a parameter set requires having the Supervisor user status and is only possible if there is no rack in the instrument and the instrument is idle.

  • Page 67: Creating A New Parameter Set

    1. In the parameter set menu (→ Fig. 44), tap on the corresponding entry to select the parameter set to which a rack handle color is to be assigned (→ Fig. 44-1). 2. Pressing the Color (→ Fig. 44-2) button displays a selection field (→ Fig. 45), that allows the assignment of the rack handle color to the selected parameter set. HistoCore SPECTRA CV...
  • Page 68 • If slides in HistoCore SPECTRA ST are processed with a program, which was allocated a specific color (for example green), the user has to pay attention that the same color is assigned to the suitable parameter set in HistoCore SPECTRA CV which was created and is startable. Fig. 44 Note • All available colors are displayed in Figure (→ Fig. 45). If an abbreviation is entered in the color field, this color has already been assigned to a parameter set.
  • Page 69 The color white is a wildcard color. When inserting a rack with a white handle, a selection window opens that has to be used to assign a unique parameter set to the white handle. Assigning the color white to a parameter set removes all colors previously assigned to that parameter set. HistoCore SPECTRA CV...

  • Page 70: Properties Of The Mounting Medium

    Operation 5.9.3 Properties of the mounting medium Note Leica provides validated mounting media for the HistoCore SPECTRA CV. This offers the user the following advantages: • Easy handling and non-contact filling and/or changing of the mounting medium • Low defect rate • Closed system • Fully automated importing of relevant data (designation, LOT numbers, number of possible coverslipping operations remaining) into CMS, •...

  • Page 71: Adjustment Of The Application Volume

    Position on the slide, coverglass size of 50 mm Label field of the slide 5.9.5 Adjustment of the application volume The HistoCore SPECTRA CV has a volume setting for the application volume of the mounting medium that is set by default and validated by Leica. The default values are defined as 0 (=default) in the setting for the parameter set (→ Fig. 47-1). These settings can be used immediately.

  • Page 72: Reagent Vessels In The Load Drawer

    • If the instrument is to be in a non-running state for a moderate period of time or if the HistoCore SPECTRA CV is not to be used for a long period of time (longer than 5 days), cover or empty the reagent vessels in the load drawer in order to prevent evaporation of the filled reagent and to prevent the formation of vapors.
  • Page 73 (→ P. 67 – 5.9.1 Creating a new parameter set) or to assign the color to an appropriate existing parameter set (→ P. 67 – 5.9.2 Assigning a parameter set to a rack handle color). HistoCore SPECTRA CV...

  • Page 74: Module Status

    Operation 5.11 Module status • The menu Module Status (→ Fig. 49) provides the user in HistoCore SPECTRA CV an overview of the used consumables (mounting media (→ Fig. 49-1) and coverglass (→ Fig. 49-3)) and their current fill levels (→ Fig. 49-5) (→ Fig. 49-6) and remaining amounts (→ Fig. 49-2) (→ Fig. 49-4) for both coverslip lines.

  • Page 75: Daily Instrument Setup

    The user is responsible for monitoring. • Before the daily instrument setup, ensure that the covers of the reagent vessels in the load drawer were removed and the drawer inserts are correctly inserted in the unload drawer (→ Fig. 8-1). HistoCore SPECTRA CV...

  • Page 76: Switching On And Shutting Down The Instrument

    Daily Instrument Setup Switching on and shutting down the instrument Switching on the instrument 1. To start the instrument, press the Operating switch (→ Fig. 52-1) illuminated in red. 2. During initialization, a verification of all modules and consumables is carried out automatically.  The Operating switch (→ Fig. 52-1) is illuminated in green whenever the instrument is ready to start.

  • Page 77: Checking And Refilling Consumables

    During initialization of the instrument, the consumables (mounting medium, coverglass, fill level of the needle cleaning container) are checked automatically (→ P. 43 – 5.4 Consumables Management System (CMS)). Fig. 53 • If a consumable is used up, a corresponding warning message is sent to the user, for example (→ Fig. 54). Fig. 54 HistoCore SPECTRA CV...

  • Page 78: Changing The Mounting Medium Bottle

    Daily Instrument Setup Note In the event that there is insufficient mounting medium available in order to fill the hose after initialization, a new mounting medium bottle must be inserted by the user (→ P. 78 – 6.3.1 Changing the mounting medium bottle). • To avoid air bubbles in the system, prime the system after initialization of the instrument (→...
  • Page 79 Fig. 56 5. Take a new mounting medium bottle (→ Fig. 57) out of the packaging and remove the black plastic cap (→ Fig. 57-1). Warning Do not remove the white protective foil (→ Fig. 57-2). It must remain on the bottle. HistoCore SPECTRA CV...
  • Page 80 Daily Instrument Setup Fig. 57 6. Insert the new mounting medium bottle (→ Fig. 58-1) into the slot (→ Fig. 58-2) in the bottle sledge and make sure that the RFID chip attached to the bottle is seated in the notch of the slot (→ Fig. 58-3). 7.

  • Page 81: Monitoring And Refilling Of The Needle Cleaning Container

    10. Take the needle out of the prime bottle and reinsert it into the holder. 11. In the Maintenance menu, press the Close button. 12. Close the hood. 13. The needle is automatically immersed in the needle cleaning container. HistoCore SPECTRA CV...

  • Page 82: Checking And Replacing The Coverglass Cartridge

    While changing the coverglass cartridge, always wear personal protective clothing (cut-resistant gloves, safety goggles)! Note • For the HistoCore SPECTRA CV Leica-validated coverglass (→ P. 158 – Consumables) available. The coverglasses are only available in cartridges which are equipped with a RFID chip.
  • Page 83 These are added on to the new coverglass cartridge and displayed in the Module Status. • Ensure that the coverglass is inserted correctly in the magazine (→ Fig. 64). 4. Dispose of the empty coverglass cartridge in accordance with the laboratory regulations. HistoCore SPECTRA CV...
  • Page 84 Daily Instrument Setup Fig. 61 5. Unpack a new coverglass cartridge (→ Fig. 62-1) and remove both the transport anchor (→ Fig. 62-2) and the silica gel packet (→ Fig. 13-3). 6. Insert the new coverglass cartridge into the slot for the coverglass cartridge (→ Fig. 62-3). Fig. 62 7. Close the instrument hood. 8.
  • Page 85  The data from the new coverglass cartridge is now imported and the Module Status is updated. Fig. 64 Warning Wear cut-resistant safety gloves (→ P. 18 – 3.1 Standard delivery – packing list) when inserting additional coverglass! The maximum filling level of the coverglass cartridge (see marking within the magazine) must not be exceeded in order to prevent instrument faults. HistoCore SPECTRA CV...

  • Page 86: Emptying The Waste Tray

    Daily Instrument Setup 6.3.4 Emptying the waste tray Note The HistoCore SPECTRA CV automatically detects defective/broken coverglass and places it in the waste tray. Warning Wear cut-resistant safety gloves (→ P. 18 – 3.1 Standard delivery – packing list) for emptying the waste tray! 1.

  • Page 87: Load Drawer

    Check that the fill level is sufficient (→ Fig. 67) and the reagent is free of dirt. In the event of contamination, the xylene is to be replaced (→ P. 37 – 4.6.3 Prepare the reagent vessel, fill it and insert it into the load drawer) and the reagent vessel is to be cleaned before filling it again. Warning A reagent vessel fill level that is too low can cause losses in quality during coverslipping. 5. A sufficient filling level is ensured if the level of the reagent is between the maximum (→ Fig. 67-1) and the minimum (→ Fig. 67-2) fill level mark. HistoCore SPECTRA CV...

  • Page 88: Unload Drawer

    Daily Instrument Setup Fig. 67 6. If the filling level is below the minimum mark, the reagent vessel has to be refilled with the same reagent (→ P. 32 – 4.6 Refilling consumables). Note • Always fill reagent vessels with xylene. Blending with other reagents shall be avoided. • Cover the reagent vessels at the end of the work day to prevent evaporation of the filled reagent. 6.3.7 Unload drawer 1. Press the drawer button on the unload drawer (→ Fig. 50-2). 2. The unload drawer opens. 3. Remove any racks that may be present in the unload drawer. 4.

  • Page 89: Preparing The Rack

    • The instrument software detects the manual insertion of racks for 5 slides in the load drawer of the HistoCore SPECTRA CV, and an information message prompts the user to remove this rack again. • If racks for 5 slides are used for staining in the HistoCore SPECTRA ST, select the unloader as the last station in the program.
  • Page 90 Daily Instrument Setup The colored handles of the racks must be attached corresponding to the previously defined parameter set color (→ P. 67 – 5.9.2 Assigning a parameter set to a rack handle color). The slide handles are available in 9 colors (8 parameter set colors & white) (→ P. 153 – 9.1 Optional accessories). Note Special function of the white handle: •...
  • Page 91 (xylene) resistance prior to use. • Make sure that only one slide each is put into the slot and that the slides are not jammed. Slots must not be empty between the two slides. HistoCore SPECTRA CV...

  • Page 92: Brief Inspection Before Starting The Coverslipping Operation

    Daily Instrument Setup Brief inspection before starting the coverslipping operation The following points have to be checked again before starting the coverslipping operation: • Before starting the coverslipping operation, remove the covers of the reagent vessels in the load drawer and check the level. If necessary, refill xylene. The level should reach up to the label field on the slide for an inserted rack.
  • Page 93 15. When the drying time period is complete, the rack is transported out of the oven and into the unload drawer by the gripper, and is put down in one of the three rear positions. 16. The user will be notified via an information message and can carefully remove the rack out of the unload drawer. HistoCore SPECTRA CV...

  • Page 94: Starting The Coverslipping Operation

    Daily Instrument Setup Note If the oven step has been disabled or if the oven is completely shut down, the rack is transported – by the gripper – directly from the elevator into the unload drawer. Proceed carefully while removing out of the unload drawer, since the mounting medium is not yet completely dry and in the event of improper removal, coverglass may shift out of place.
  • Page 95 5. Press the load drawer button again to close it again. 6. After closing the load drawer, the instrument detects the RFID chip in the colored handle. 7. The detected handle color is shown in the process status display (→ Fig. 73).  Processing starts automatically. Fig. 73 HistoCore SPECTRA CV...
  • Page 96 Daily Instrument Setup Note • The slides are not processed sequentially, i.e. they are not coverslipped in sequence. • The schematic display (→ Fig. 74) of a rack (→ Fig. 74-1) with inserted slides (→ Fig. 74-2) shows the sequence during processing. • The first five slides are coverslipped at the beginning (→ Fig. 74-3). Then the rack moves upwards and continues the coverslipping with the lowest slide (→ Fig. 74-4).

  • Page 97: Monitoring The Coverslipping Operation

    • If a rack ran the coverslipping operation, it will be transported in the unload drawer. • The unload drawer can hold up to 9 racks. The racks are placed in the unload drawer in the sequence shown from A1 to C9 (→ Fig. 75). Fig. 75 HistoCore SPECTRA CV...
  • Page 98 3 days. This applies to both HistoCore SPECTRA ST and HistoCore SPECTRA CV stand-alone units as well as in operation as a workstation. This also implies the HistoCore SPECTRA CV must be restarted by the user at least every 3 days.

  • Page 99: Pausing Or Canceling The Coverslipping Operation

    (→ P. 131 – 8. Malfunctions and Troubleshooting). Canceling the coverslipping operation L Coverslipping operations can only be canceled using the process status display (→ Fig. 78). 1. To cancel a coverslipping operation, select the corresponding rack (→ Fig. 78-1) by touching it. HistoCore SPECTRA CV...
  • Page 100 Daily Instrument Setup Fig. 78 2. An overview appears of the rack for which the process is being canceled (→ Fig. 79). 3. The border of the window shows the color assigned to the parameter set, the mounting medium used, the coverslip size used and the stored volume. 4.

  • Page 101: Workstation Operation

    • If the HistoCore SPECTRA CV cannot accept a rack from the HistoCore SPECTRA ST (e.g. due to an instrument fault in the HistoCore SPECTRA CV or if the load drawer of the HistoCore SPECTRA CV is assigned), these will be transported to the HistoCore SPECTRA ST unload drawer.
  • Page 102 HistoCore SPECTRA CV by means of the transfer station and placed into a reagent vessel for the load drawer of the HistoCore SPECTRA CV. In the Instructions for Use for the HistoCore SPECTRA ST, the notes on program creation must be observed.
  • Page 103 On the HistoCore SPECTRA ST status display, a symbol indicates whether there is a connection to a HistoCore SPECTRA CV or if the connection has been interrupted. The connection between the HistoCore SPECTRA ST and the HistoCore SPECTRA CV has been established.

  • Page 104: Starting The Coverslipping Operation In Workstation Mode

    2. Both the staining and coverslipping processes are controlled by the assigned rack handle color. 3. Racks where the staining and coverslipping processes have been completed are automatically placed in the unload drawer of the HistoCore SPECTRA CV and have to be removed by the user there.

  • Page 105: Cleaning And Maintenance

    After cleaning the surfaces, wipe them off with a cloth moistened with water. Warning Varnished instrument surfaces and plastic surfaces (e.g. an instrument hood) may not be cleaned with solvents like acetone, xylene, toluene, xylene substitutes, alcohol, alcohol mixtures and abrasives! In case of long-term exposure, the surfaces and instrument hood are only conditionally resistant to solvents. HistoCore SPECTRA CV...

  • Page 106: Tft Touchscreen

    Cleaning and Maintenance 7.2.2 TFT touchscreen » Clean the screen using a lint-free cloth. A suitable screen cleaner can be used in compliance with the manufacturer information. 7.2.3 Load and unload drawers Warning • To avoid an overflowing of a filled reagents, do not open the load drawer suddenly. • Wear suitable protective clothing (lab coat, safety goggles, cut-resistant gloves). 1.

  • Page 107: Interior Cleaning

    Remove all existing racks in the load and unload drawers before cleaning (→ Fig. 83). Fig. 83 2. Carefully remove any dirt remnants. 3. Pull the retaining lever for the unload drawer (→ Fig. 84-1) upwards while slowly continuing to open the drawer as far as it will move. HistoCore SPECTRA CV...
  • Page 108 Cleaning and Maintenance Fig. 84 4. Carefully fold the unload drawer down and support it with one hand to allow you to clean the rear area inside the instrument (→ Fig. 85). Fig. 85 5. Soak a lint-free cloth with xylene and remove any mounting medium residue that may be present. You can use a commercially available vacuum cleaner to pick up any non-adhesive glass shards, splinters or dust.

  • Page 109: Cleaning The Prime Bottle

    (→ Fig. 86-2) and hook it into a container filled with xylene. Let it soak shortly (approx. 10 min.). Fig. 86 4. Then remove the needle from the xylene, moisten it with xylene and wipe off the residual mountant with a soft, lint-free cloth. 5. Finally, insert the needle (→ Fig. 87-1) back into its holder (→ Fig. 87-2). HistoCore SPECTRA CV...

  • Page 110: Filling And Changing The Needle Cleaning Container

    Cleaning and Maintenance Fig. 87 Note The needle has a notch (→ Fig. 87-3) that fits exactly in the holder. The Attention symbol (→ Fig. 87-4) on the holder (→ Fig. 87-2) indicates to the user that utmost care is required when inserting the needle into the holder. The needle must be inserted straight and all the way in order to make sure that no negative impact on the samples arises during processing.

  • Page 111: Removing The Complete Unit Of The Needle Cleaning Container

    3. The needle moves upwards out of the needle cleaning container. 4. Remove the needle from the holder and insert it into the prime bottle. 5. Open the load and unload drawers completely and remove all racks. HistoCore SPECTRA CV...
  • Page 112 Cleaning and Maintenance Note The unload drawer is capable of being unlocked and folded down so the user has easier access to the interior of the instrument (→ P. 107 – 7.2.4 Interior cleaning). In doing so, make sure that the inserts do not fall out into the unload drawer.
  • Page 113 (→ P. 81 – 6.3.2 Monitoring and refilling of the needle cleaning container) and insert it into the complete unit. 15. Insert the needle from the prime bottle back into the needle holder and press the Close button in Prime/Clean menu. 16. Finally, close the hood. HistoCore SPECTRA CV...

  • Page 114: Cleaning The Pick&Place Module

    Cleaning and Maintenance 7.2.10 Cleaning the Pick&Place module • For handling the Pick&Place module, see (→ P. 86 – 6.3.5 Inspect Pick&Place module). Clean the following areas of the Pick&Place module with a lint-free cloth soaked with xylene: 1. Check the front and rear suction cups (→ Fig. 91-2) at the Pick&Place module for dirt and damages.

  • Page 115: Cleaning The Waste Tray

    For handling, removing and re-inserting the waste tray, see (→ P. 86 – 6.3.4 Emptying the waste tray). » Soak a lint-free cloth with xylene and remove any mounting medium residue that may be present. Note Dispose of glass shards, splinters and dust in accordance with laboratory specifications. HistoCore SPECTRA CV...

  • Page 116: Cleaning The Reagent Vessels

    Cleaning and Maintenance 7.2.13 Cleaning the reagent vessels 1. Remove the reagent vessels individually from the load drawer by the handle. Ensure that the handle is in the correct position (→ P. 37 – 4.6.3 Prepare the reagent vessel, fill it and insert it into the load drawer) to prevent spilling the reagent. 2.

  • Page 117: Changing The Active Carbon Filter

    3. Write the insertion date on the white adhesive label on the front side of the activated carbon filter (→ Fig. 93-3). 4. Insert the new activated carbon filter in such a way that the pull tab is visible and reachable even after it is completely pushed in. 5. The active carbon filter must be pushed in until you feel it make contact with the rear panel of the instrument. 6. Close the cover (→ Fig. 93-2) again. Fig. 93 HistoCore SPECTRA CV...

  • Page 118: Cleaning Reagent Vessels In The Load Drawer

    Cleaning and Maintenance 7.2.16 Cleaning reagent vessels in the load drawer • Open the load drawer and individually remove the reagent vessels by the handle. Ensure that the handle is in the correct position (→ P. 37 – 4.6.3 Prepare the reagent vessel, fill it and insert it into the load drawer) to prevent spilling the reagent. •...
  • Page 119 Cleaning and Maintenance Fig. 94 Fig. 95 3. Observe the following information message (→ Fig. 96). Fig. 96 HistoCore SPECTRA CV...
  • Page 120 Cleaning and Maintenance 4. Open the instrument hood and remove the needle (→ Fig. 97-1) from the holder (→ Fig. 97-2). 5. Stick the needle unit (→ Fig. 97-1) in the prime bottle (→ Fig. 97-3) and acknowledge the information message (→ Fig. 96) with Warning Do not close the hood while the needle is inserted in the prime bottle in order to avoid kinking the hose or bending the needle.

  • Page 121: Quick Prime

    2. Priming lasts approx. 35 seconds and uses approx. 2 ml of mounting medium. This quantity is taken into account by the CMS. 3. After the rinsing process has been completed, reinsert the needle unit into the holder (→ Fig. 87) and confirm the corresponding information message with OK. HistoCore SPECTRA CV...

  • Page 122: Extended Prime

    Cleaning and Maintenance Note The needle has a notch (→ Fig. 87-3) that fits exactly in the holder. The Attention symbol (→ Fig. 87-4) on the holder (→ Fig. 87-2) indicates to the user that utmost care is required when inserting the needle into the holder. The needle must be inserted straight and all the way in order to make sure that no negative impact on the samples arises during processing.

  • Page 123: Cleaning The Hose System

    (increased bubble formation between the slide and coverslip) or if the HistoCore SPECTRA CV is to be prepared for transport or not in use for a prolonged time frame (longer than 5 days). The mounting medium in the hose system is washed out using a cleaning solution.
  • Page 124 Cleaning and Maintenance 7. Take the mounting medium bottle out of the bottle sledge, close and store safely. 8. Fill the cleaning bottle (→ P. 18 – 3.1 Standard delivery – packing list) with at least 125 ml of cleaning solution (xylene) while it is outside the instrument and place it in the position of the mounting medium bottle in the bottle sledge.
  • Page 125 » Following this, the user is prompted to remove the cleaning bottle from the instrument and to insert a new bottle with mounting medium. After the insertion, acknowledge the information message (→ Fig. 104) with Ok. Dispose of the contents of the cleaning bottle in accordance with laboratory regulations. Fig. 104 HistoCore SPECTRA CV...
  • Page 126 Cleaning and Maintenance Cleaning process finished and preparation for transport or storage 1. After the cleaning process has been completed, remove the prime bottle from the instrument and dispose of the contents according to laboratory specifications. 2. Insert the empty prime bottle back into the instrument again. Note After the cleaning process has been completed, the user is asked if a new bottle of mounting medium is to be used to continue with the coverslipping (→ Fig. 103).

  • Page 127: Recommissioning After Transport Or Storage

    • It is mandatory to observe and carry out the following cleaning and maintenance intervals and cleaning tasks. • In case of non-compliance with the maintenance intervals, the proper function of the HistoCore SPECTRA CV by Leica Biosystems GmbH cannot be guaranteed. • The instrument should be inspected once per year by a qualified service technician authorized by Leica.

  • Page 128: Daily Cleaning And Maintenance

    Cleaning and Maintenance 7.4.1 Daily cleaning and maintenance Coverglass cartridge: • Check the coverglass cartridge for broken glass (→ P. 82 – 6.3.3 Checking and replacing the coverglass cartridge) and clean if necessary. • Check that the coverglass is seated correctly in the coverglass cartridge (→...

  • Page 129: Weekly Cleaning And Maintenance

    (→ P. 107 – 7.2.4 Interior cleaning). Warning Never place reagent vessels, racks and rack handles in a solvent (xylene) for a prolonged period (e.g. overnight) because this can cause deformation and trouble-free operation can no longer be ensured. HistoCore SPECTRA CV...

  • Page 130: Quarterly Cleaning And Maintenance

    Cleaning and Maintenance 7.4.3 Quarterly cleaning and maintenance Changing the active carbon filter: • Replace the active carbon filter (→ P. 117 – 7.2.15 Changing the active carbon filter). 7.4.4 Cleaning and maintenance as necessary Warning • Attention: Wear cut-resistant gloves (→ P. 18 – 3.1 Standard delivery – packing list)! • Clean the interior of the instrument in the event of broken glass. •...

  • Page 131: Malfunctions And Troubleshooting

    • Follow the instructions in an error (→ P. 138 – 8.3 Manual removal of a rack in the event of instrument malfunctions) and pull the transport arm to the unload drawer. Remove the rack from the transport arm. HistoCore SPECTRA CV...
  • Page 132 • Ensure that xylene is being used both in the reagent vessel in the HistoCore SPECTRA CV load drawer and in the last steps of the preceding staining process in the HistoCore SPECTRA ST. • Dispose of non-compatible reagent...
  • Page 133 • coverglass in the magazine have • Remove the cover slip magazine been moistened and can no and replace it with a new one longer be removed individually. (→ P. 82 – 6.3.3 Checking and replacing the coverglass cartridge). HistoCore SPECTRA CV...
  • Page 134 Malfunctions and Troubleshooting Problem/malfunction Possible cause Correction Drying out of the needle • Malfunction in a coverslip line, • Remove the needle from the the needle could not be moved to needle holder and set it in the the needle cleaning container. needle cleaning container.
  • Page 135 • Check rack for uncoverslipped removed too many coverglasses slides! after the message of detecting too many coverglasses in the magazine. Note Display messages for certain failures contain image sequences to guide the user through the troubleshooting process (→ Fig. 107). Fig. 107 HistoCore SPECTRA CV...

  • Page 136: Power Failure Scenario And Instrument Failure

    Note • In the event of a prolonged power failure (> 3 sec.), the internal battery ensures that the HistoCore SPECTRA CV is shut down in a controlled manner and any negative impact on the specimens is avoided. • The fully charged internal battery can bridge two successive power failures (> 3 sec.). After two successive power failures and once the power supply has been re-established, the internal battery is recharged when the instrument is started up. The user is notified of the charging process by a...
  • Page 137 (→ P. 138 – 8.3 Manual removal of a rack in the event of instrument malfunctions) Oven (→ 8.3.4 Removing the rack from the oven or from behind the oven) Unload drawer (→ P. 138 – 8.3 Manual removal of a rack in the event of instrument malfunctions) HistoCore SPECTRA CV...

  • Page 138: Manual Removal Of A Rack In The Event Of Instrument Malfunctions

    Malfunctions and Troubleshooting Manual removal of a rack in the event of instrument malfunctions Warning • Risk of injury! Always wear personal protective clothing (lab coat, cut-resistant gloves, safety goggles)! • We recommend calling in a second person for support. • The instrument software provides instructions on the screen. Compliance with these is mandatory. •...
  • Page 139 Malfunctions and Troubleshooting Note Place the extracted rack outside of the instrument and secure the slide against drying out. Fig. 109 HistoCore SPECTRA CV...

  • Page 140: Malfunction At The Coverglass Receptacle

    Malfunctions and Troubleshooting 8.3.1 Malfunction at the coverglass receptacle If the Pick&Place module loses a coverglass during transport to the slide or if the Pick&Place module cannot remove a coverglass from the coverglass cartridge after 3 attempts, the user is notified of this through an error message (→ Fig. 110). Fig. 110 Note Processing in the respective coverslip line is interrupted in order to prevent damage to the specimens. Specimens may become dried out during the interruption.
  • Page 141 10. If the processing was interrupted for too long, it can be canceled by pressing (→ Fig. 112-1). The rack in question is transported to the unload drawer and can be removed by the user there. 11. To continue processing, press the (→ Fig. 112-2) button. Processing is continued as normal. HistoCore SPECTRA CV...

  • Page 142: Removing A Rack From The Elevator Of The Coverslip Line

    Malfunctions and Troubleshooting Fig. 112 Note If the error cannot be remedied by the user using the specified procedure, notify a Leica service technician and remove the coverslip line in question from service for the time being. 8.3.2 Removing a rack from the elevator of the coverslip line Warning • If, during operation, a malfunction occurs in one of the two coverslip lines, the user will receive information messages from the instrument, which will provide the user with instructions for the potentially necessary removal of a rack from the elevator.
  • Page 143 Note If the instrument can not move the rack to the uppermost position (due to i.e. blocking slides), the following three steps have to be performed before attempting to remove the rack. HistoCore SPECTRA CV...
  • Page 144 Malfunctions and Troubleshooting 9. If a slide has canted in the coverslip line (→ Fig. 115-1), push the shifter (→ Fig. 115-2) forwards. To do so, press the red bar to the far right (→ Fig. 111-1) and hold it in this position. Then carefully pull the canted slide forwards (→ Fig. 115-3), remove it from the instrument (→ Fig. 115-4) and coverslip it manually.
  • Page 145 (→ Fig. 118-3). 14. Continue to hold the elevator (→ Fig. 118-4) and remove the rack from the elevator with the other hand (→ Fig. 118-5). Make sure that no slides slip out of the rack. 15. Now release the elevator, take the rack out of the instrument and confirm the message. Fig. 118 HistoCore SPECTRA CV...
  • Page 146 Malfunctions and Troubleshooting 16. Follow the instructions on the display and close the locking mechanism of the elevator again (→ Fig. 119-1) and push the elevator a few centimeters downward (→ Fig. 119-2). Confirm the message after completing the task. Fig. 119 17. The next message asks the user to remove the needle from the parking location (→ Fig. 120-1) and insert it into the holder (→ Fig. 120-2)

  • Page 147: Removing The Rack From The Lower Area Of The Left Elevator

    3. Press the rack downwards and out of the elevator (hold the holder from below using your little finger) and push it toward the back. 4. The rack can also be removed upwards using the coverslip line and the elevator. To do so, please note the action as described in step 2. HistoCore SPECTRA CV...

  • Page 148: Removing The Rack From The Oven Or From Behind The Oven

    Malfunctions and Troubleshooting 8.3.4 Removing the rack from the oven or from behind the oven Warning • Danger of explosion! Easily flammable reagents in the oven • There is a potential for respiratory tract irritation through evaporating flammable reagents. • Caution! Hot surfaces! The oven door and the interior of the oven are hot. Avoid contact if possible to prevent burns. 1. Open access to the oven (→ Fig. 122-1). 2. Open the oven door (→ Fig. 122-2) fully upwards (→ Fig. 122-3), the magnet holds the oven door in place (→ Fig. 122-4).
  • Page 149 Malfunctions and Troubleshooting Fig. 122 HistoCore SPECTRA CV...

  • Page 150: Removal Of The Rack From The Rotator

    8.3.7 Removal of a rack from the transfer station of the HistoCore SPECTRA ST L If a malfunction occurs in workstation mode while the transfer device of the HistoCore SPECTRA ST transfers a rack to the HistoCore SPECTRA CV using the transfer station, the user must check where the rack is located.
  • Page 151 Fig. 125 4. Following this, remove the rack from the instrument and store it safely. 5. After the instrument fault has been corrected, insert the rack in the load drawer of the HistoCore SPECTRA CV to start processing. HistoCore SPECTRA CV...

  • Page 152: Replacing Main Fuses

    Malfunctions and Troubleshooting Replacing main fuses Warning The instrument should be shut down using the Main Switch and disconnected from the power supply in the case of a instrument failure. The main fuses can then be inspected. A suitable flat-head screwdriver must be used to prevent damage to the fuse holders. Beware of defective fuses! Potential risk of injury due to broken glass! Wear suitable protective clothing (safety goggles, cut-resistant gloves (→...

  • Page 153: Optional Accessories And Consumables

    Exhaust air hose, 2 m 14 0422 31974 Hose clamp 14 0422 31973 Cut-resistant gloves, size M, 1 pair 14 0340 29011 Tool kit HistoCore SPECTRA CV, consisting of: 14 0514 54189 Screwdriver, 5.5x150 14 0170 10702 Leica brush 14 0183 30751...
  • Page 154 Optional Accessories and Consumables Exhaust hose 1 set, consisting of the following: • Exhaust hose of length: 2 m • Hose clamp Order No.: 14 0422 31974 Fig. 127 Active carbon filter 1 set, consisting of 2 pcs. Order No.: 14 0512 53772 Fig. 128 Waste tray Order No.:...
  • Page 155 Optional Accessories and Consumables Needle cleaning container 1 kit, consisting of 2 pcs. Order No.: 14 0514 54195 Fig. 130 Reagent vessel Assembly, incl. reagent vessel cover Order No.: 14 0512 47086 Fig. 131 HistoCore SPECTRA CV...
  • Page 156 Optional Accessories and Consumables Racks 30 specimen slides (3 pcs. per package) Order No.: 14 0512 52473 Fig. 132 Handle for racks 30 specimen slides (3 pcs. per package) Color Order No.: • yellow 14 0512 52476 • light blue 14 0512 52477 •...
  • Page 157 Cleaning bottle Assembly Order No.: 14 0514 57248 Fig. 135 Suction cup 1 pcs. Order No.: 14 3000 00403 Fig. 136 Storage rails for the unload drawer 1 set, consisting of 3 pcs. Order No.: 14 0514 56165 Fig. 137 HistoCore SPECTRA CV...
  • Page 158 Order No.: 14 0475 58558 Fig. 140 Consumables Designation Order No. mounting medium HistoCore SPECTRA X1 (1 packaging unit, 2 bottles with 150 ml each) 380 1733 Coverglasses HistoCore SPECTRA CV premium coverglass 1x24x50 (8x300 pcs.) 380 0152 Version 2.2, Revision L...

  • Page 159: Warranty And Service

    Warranty and Service 10. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and reflects all technical specifications and/or agreed characteristics. The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively.

  • Page 160: Decommissioning And Disposal

    Certificate (→ P. 161 – 12. Decontamination Certificate) at the end of these Instructions for Use for notes on cleaning the HistoCore SPECTRA CV coverslipper. To reduce potential contamination, thorough disinfecting of the instrument is required before re- commissioning or disposal (e.g. multiple cleaning steps, disinfection or sterilization). Dispose of the instrument in compliance with the applicable laboratory specifications.

  • Page 161: Decontamination Certificate

    Decontamination Certificate 12. Decontamination Certificate Any product that is to be returned to Leica Biosystems or serviced on site must be properly cleaned and decontaminated. The associated decontamination certificate template can be found on our website www. LeicaBiosystems.com in the product menu. This template must be used to enter all required data. If a product is returned, a copy of the completed and signed decontamination certificate must either be enclosed or handed over to a service technician. The user shall be responsible for products that are returned without a completed decontamination certificate or with a missing decontamination certificate.
  • Page 162 Notes Version 2.2, Revision L...
  • Page 164 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 - 19 69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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