Leica BIOSYSTEMS HistoCore PEARL Instructions For Use Manual
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Leica BIOSYSTEMS HistoCore PEARL Instructions For Use Manual

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HistoCore PEARL
Tissue Processor
Instructions for Use
English
Order No.: 14 0493 80101 - Revision D
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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Summary of Contents for Leica BIOSYSTEMS HistoCore PEARL

  • Page 1 HistoCore PEARL Tissue Processor Instructions for Use English Order No.: 14 0493 80101 - Revision D Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the nameplate on the back of the instrument.

  • Page 4: Table Of Contents

    Table of Contents Important Information ..........................Symbols used in the text and their meanings ..................... 7 Instrument type .............................. 10 Intended use ..............................10 Qualification of personnel ..........................10 Safety ................................Safety notes ..............................11 Warnings ................................. 12 2.2.1 Markings on the instrument itself ....................... 12 2.2.2 Transport and Installation ..........................
  • Page 5 5.4.2 Favorite programs ............................72 Dashboard ............................... 73 Reagent handling ............................76 5.6.1 Filling/draining reagents ..........................76 5.6.2 Filling/refilling paraffin ..........................77 Starting a program ............................79 Ending a program ............................84 Pre-installed program ........................... 84 Cleaning and maintenance ........................HistoCore PEARL...
  • Page 6 Table of Contents Cleaning program ............................87 General instrument cleaning ........................88 6.2.1 Daily cleaning and maintenance......................... 88 6.2.2 Weekly cleaning and maintenance ......................90 6.2.3 Monthly cleaning and maintenance ......................91 6.2.4 Recommended cleaning reagents ......................92 Checklist for regular maintenance ......................92 Troubleshooting ............................

  • Page 7: Important Information

    Observe the Instructions for Use Caution, consult the instructions for use for cautionary information. Caution, consult the instructions for use for cautionary information. In vitro diagnostics (IVD) medical device This product fulfills the requirements of the EU directives. HistoCore PEARL...
  • Page 8 Important Information Symbol for labeling electrical and electronic equipment in accordance with Section 7 of the German Electrical and Electronic Equipment Act (ElektroG). ElektroG is the law regarding the sale, return and environmentally sound disposal of electrical and electronic equipment. Environmental protection symbol of the China RoHS directive.
  • Page 9 Improper handling of the shipment is immediately detectable and can be proven definitively. This product fulfills the requirements of the CAN/CSA-C22.2 No. 61010. Indicates the item can be recycled where correct facilities exist. HistoCore PEARL...

  • Page 10: Instrument Type

    A nameplate indicating the instrument serial number is attached to the rear side of the instrument. Intended use The HistoCore PEARL is designed to enable tissue processing, performing all steps from fixation to wax infiltration. It is normally processed in sequential mode, which means that the instrument is strictly using one bottle after the other.

  • Page 11: Safety

    • Only original spare parts and permitted original accessories can be used. • If the instrument is to be returned to Leica Biosystems for repair, it must be cleaned and decontaminated in the appropriate manner. For details, please see (→...

  • Page 12: Warnings

    • The installation location must be well-ventilated; there should be no ignition sources there of any kind. The chemicals to be used in the HistoCore PEARL are both flammable and noxious. • Do not operate the instrument in rooms with explosion hazard.

  • Page 13: Operating The Instrument

    • Failure to observe the instructions specified by the manufacturer may result in damage to the protection provided by the instrument. • Pre-installed Leica Biosystems programs are validated based on Leica Biosystems consumables and reagents. • Before using the instrument, run the Bottle Check function to check the connections of the reagent bottles.

  • Page 14: Handling Reagents

    Compatible reagents)! • Do not use acetone, benzene, chloroform and trichlorethane on HistoCore PEARL. • When disposing of spent reagents, observe the applicable local regulations and the waste disposal regulations of the company/institution in which the instrument is being operated.

  • Page 15: Cleaning And Maintenance

    Safety features on the instrument The HistoCore PEARL tissue processor is equipped with safety functions and with sophisticated software control mechanisms. These ensure that the specimens remain undamaged in the event of a power failure or other malfunction during the program and that the program is completed successfully.

  • Page 16: Instrument Components And Specifications

    Instrument Components and Specifications Instrument Components and Specifications Overview Instrument components Fig. 1 Active carbon filter Caster Specimen baskets Condensate bottle (1) Retort Paraffin drip tray Lid latch knob Paraffin oven Paraffin baths (3) Basic instrument Paraffin oven cover Counter area Reagent bottles (13) Display Bottom tray...

  • Page 17: Main Features Of The Instrument

    Main features of the instrument • The HistoCore PEARL is equipped with an LCD touch screen. Multilingual user interface and graphic display of the program flow (dashboard) are for clarifying all menu steps. • The HistoCore PEARL can be operated using customized or pre-installed, validated tissue processing programs.

  • Page 18: Technical Data

    • Access to the instrument can be limited via a multilevel password protection system. • The user can utilize the Bottle Check function to ensure connecting status of reagent bottles. • In the event of a power failure, the alarm system is triggered off. The HistoCore PEARL will provide recovery measures after interruption.
  • Page 19 Alphanumeric, freely selectable Hardware and Software • Color LCD touchscreen. • User-friendly, interactive software. • Two USB 2.0 ports, DC 5V. • Alarm system with two alarm sockets (for local alarm and remote alarm). • Smart error-handling for specimen protection. HistoCore PEARL...

  • Page 20: Compatible Reagents

    Compatible reagents Caution Use of the HistoCore PEARL is permitted with the reagents specified in this section only. These reagents must be validated before use, i.e. tissue processing with patient tissue for diagnostics, by the laboratory itself according to the local or regional accreditation requirements.

  • Page 21: Recommended Reagent Handling

    If you choose to work with such fixatives, change the processing water at least every 600 cassettes or 3 cycles, to minimize the damage. In addition, we recommend regular preventive maintenance by Leica Biosystems Technical Service. Caution Reagents other than these listed here may damage components of the instrument. Do not use acetone, benzene chloroform and trichlorethane in the instrument.
  • Page 22 Instrument Components and Specifications Station Reagent *Week 1/ Week 2 Week 3 Week 4 5 Cycles Move to Move to Move to Move to Xylene Station 9 Station 9 Station 9 Station 9 Xylene Move to Move to Move to Move to Station 10 Station 10...

  • Page 23: Initial Operation

    • Avoid vibrations, direct sunlight and heavy variation in temperature. Caution The chemicals used in the HistoCore PEARL are easily inflammable and hazardous to health. Therefore the installation location must be well ventilated, and must contain no open flames. The room in which the instrument is located must not be used as a permanent station for people.

  • Page 24: Unpacking And Installation

    Caution At least TWO persons are required when unpacking the instrument. If the instrument is moved over stairs, please call your local Leica Biosystems Technical Service, who can safely transport it with appropriate equipment. Caution DO NOT use the handles on the rear panel to carry, as they are not designed to bear the weight, but only for rolling the instrument.
  • Page 25 Initial Operation Fig. 3 HistoCore PEARL...
  • Page 26 9. Push the instrument from the pallet. Caution The instrument casters move very easily. The empty weight of the HistoCore PEARL is 140 kg! Therefore, it is mandatory to have at least TWO persons hold the instrument when it is rolled off the pallet via the ramp.
  • Page 27 (→ "Fig. 6-1") from behind the paraffin oven door along with the packaging (→ "Fig. 6-2") covering the reagent bottles. 12. Check the instrument and accessories for any visible transport damage. All accessories are located in the small package box. HistoCore PEARL...

  • Page 28: Basic Instrument/Hardware

    Initial Operation Additional reagent bottle Maintenance kit, Paraffin scraper, Reagent bottle labels Alarm connector Basket assembly Display module Paraffin drip tray Funnel Bottom tray Power cord Note During unpacking, make sure to keep the accessory box in the upright direction as indicated by the transportation symbol on the packaging box.

  • Page 29: The Retort

    It prevents the retort from being overfilled or insufficiently filled. There is a retort sieve (→ "Fig. 9-2") at the bottom of the retort. Install the sieve by inserting and rotating it clockwise. HistoCore PEARL...

  • Page 30: Active Carbon Filter

    Initial Operation 4.4.3 Active carbon filter Fig. 10 Inserting/Replacing the active carbon filter 1. To insert/replace the active carbon filter (→ "Fig. 10-1"), press and open the top cover (→ "Fig. 10-2") behind the retort lid. Caution Putting hand in the active carbon filter container (→...

  • Page 31: External Exhaust System

    Connect the other end of the extraction tube with the external exhaust station. Caution Even if the instrument is connected to an external exhaust device, the active carbon filter provided must remain in use. HistoCore PEARL...

  • Page 32: Counter Area

    Initial Operation 4.4.5 Counter area Fig. 12 To the right of the retort in front of the display, there is a counter area (→ "Fig. 12-1"). The flat area can be used for setting down the prepared specimens and baskets removed from the retort. However, we recommend also covering the counter area with a silicone pad (→...

  • Page 33: The Display

    4.4.7 The display Fig. 14 • The HistoCore PEARL is programmed and operated via a color LCD touchscreen. • A screen saver will turn off the screen display if no key has been pressed for 30 minutes. Press any part of the touchscreen to restore the screen. After having been restored, the on-screen functions will be non-operational for a few seconds to avoid accidental touch of any keys.

  • Page 34: The Paraffin Baths

    Initial Operation 4.4.8 The paraffin baths Fig. 15 • The instrument has three heated paraffin baths, each with a max. capacity of 3.5 L of liquid paraffin. They are located over the reagent cabinet. • The individual paraffin baths can be pulled out (→...

  • Page 35: The Paraffin Drip Tray

    It collects paraffin that have overflowed. • The paraffin drip tray must be regularly checked and cleaned. To do so, pull the paraffin drip tray out by the handle and empty it. HistoCore PEARL...

  • Page 36: Reagent Cabinet With Reagent Bottles

    Initial Operation 4.4.10 Reagent cabinet with reagent bottles Fig. 19 Fig. 20 • The 13 white plastic reagent bottles are located together with the condensate bottle (→ "Fig. 19-1") the reagent cabinet under the 3 paraffin baths (→ "Fig. 19"). The numbering of the bottle positions are silk-screened on ribs of the reagent carbinet (→...

  • Page 37: The Bottom Tray

    Before use or after maintenance, make sure the reagent bottles are properly connected. Note Only HistoCore PEARL reagent bottles can be used. Note The connection port must be pushed into place with a click sound, thus ensuring an optimum fit in the reagent cabinet.

  • Page 38: Adhesive Labels For Reagent Bottles

    Initial Operation 4.4.12 Adhesive labels for reagent bottles • The standard delivery of the HistoCore PEARL includes adhesive labels for the reagent bottles in the reagent cabinet. They can be used to mark the groups of the reagent bottles. • Ensure the surface on the reagent bottles are clean and dry; then apply the label and press on firmly—especially at the edges.

  • Page 39: Connecting The Power Supply

    Check the nameplate on the rear of the instrument to ensure that the instrument delivered is designed for the required voltage. Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set. HistoCore PEARL...

  • Page 40: Switching The Instrument On

    Switching the instrument on Caution The instrument MUST be connected to a grounded power socket. For additional electrical fuse protection, we recommend connecting the HistoCore PEARL to a socket with a residual current circuit breaker. Switching the instrument on 1. Connect the power cable to the power socket. If applicable, switch on the switch for the power socket.
  • Page 41 After having been restored, the on-screen functions will be non-operational for a few seconds to avoid accidental touch of any keys. Note When a program is running and the screen saver is active, the message "In processing" is displayed on the screen. HistoCore PEARL...

  • Page 42: Touchscreen Functions

    Initial Operation Touchscreen functions The HistoCore PEARL is operated via a touchscreen. Status Bar Fig. 28 On the status bar the following information is displayed. • Current date (→ "Fig. 28-1"). To set the current date, see (→ P. 50 – 5.2.3 Date/Time) • Current time...
  • Page 43 For the messages that cannot be cleared, the warning or error symbol will remain present in the status bar until the error is resolved by a trained service engineer. Fig. 30 HistoCore PEARL...
  • Page 44 Initial Operation Virtual keyboard Fig. 31 The keyboard (→ "Fig. 31") is displayed any time text needs to be entered. • The keyboard headline tells you what kind of text to enter. • 30 characters can be entered into each entry field, however sometimes not all characters entered can be displayed.

  • Page 45: Switching Off The Instrument

    2. Press the icon of (→ "Fig. 34-2"). Maintenance 3. Press Shutdown button (→ "Fig. 34-3"). The following message pops up. Please note that the waiting hours in the message is dependant on the settings of the paraffin melting point. HistoCore PEARL...

  • Page 46: Moving The Instrument

    5. When the following message appears, switch off the instrument with the ON/Off switch at the rear panel of the instrument. Caution The HistoCore PEARL is to be switched off completely in this way only. Otherwise, severe damage to the instrument's hardware and loss of data can occur. Note When the instrument is switched off by directly turning off the ON/OFF switch, the alarm will be triggered.

  • Page 47: Operation

    Operation Operation Access levels The HistoCore PEARL has three access levels. The user level symbol (→ "Fig. 35-1") is displayed at the upper right corner of the touchscreen. • Standard user (→ "Fig. 35-3") - The Standard user is the default user level when you log on the instrument.

  • Page 48: Setting Up System

    Operation 3. Enter new password and press OK. 4. Enter the new password again to confirm, and press OK. 5. The password is changed. Note To reset the password in case it has been forgotten, contact your local service representative. Setting up system From the start screen, press the settings icon (→...
  • Page 49 (→ "Fig. 37-2"). 3. Set the time format, 24h or 12h, by pressing the two ends (→ "Fig. 37-3"). 4. Toggle between the International format, ISO format or US format (→ "Fig. 37-4"). 5. Press (→ "Fig. 37-5"). Save HistoCore PEARL...

  • Page 50: Date/Time

    Operation 5.2.3 Date/Time View and edit the date and the time. Note It is important to set the date and time correctly so that programs start and end at the correct time and on the correct day. Fig. 38 Setting the Date/Time L Only Supervisor and Service user level can edit it.

  • Page 51: Alarm

    8. To set the alarm for the Warning, repeat from steps 2 to 7. 9. To set the alarm for the Error, repeat steps 2, 3, 4, 6 and 7. Please noted that the volume range for Error is 2-9, and the periodic time is not changeable. HistoCore PEARL...

  • Page 52: Installation

    Operation 5.2.5 Installation View the Instrument name, Serial number and Software version. Fig. 40 Viewing the Installation » Press the icon of (→ "Fig. 40-1"), and the Instrument name, Serial number and Installation Software version are displayed on the right (→...

  • Page 53: System Setup

    If the paraffin bath temperature is set from a higher value to a lower value, an error code pops up. When it occurs, restart the instrument using the On/Off button on the back panel. If any error appears after the restart, please retry after several minutes. If the problem persists, call your local service representative. HistoCore PEARL...

  • Page 54: Data Management

    Operation Bottle Check function The Bottle Check function checks the following: • The connections between the reagent bottles and the instrument for leaks and blockages; • Whether the reagent bottles are filled or insufficient. To start the Bottle Check function, press the Bottle Check button (→...
  • Page 55 L Only Supervisor and Service user level can perform this. 1. Insert a USB disk in one of the USB ports on the instrument and press the icon of Data Mgm. (→ "Fig. 42-1"). 2. Press (→ "Fig. 42-4"). Import HistoCore PEARL...

  • Page 56: Maintenance

    Operation 3. Select the file that is to import. A display on the screen shows that data is currently being copied while the files are being transferred. When all data has been successfully transferred, a completion message appears. Update software Updating software L Only Supervisor and Service user levels can perform this whilst there is not a program currently running.
  • Page 57 (→ "Fig. 43-2"). Reset Caution Only qualified service personnel authorized by Leica Biosystems can do the yearly preventive maintenance. Active carbon filter The instrument calculates the time of the active carbon filter in use. When the time exceeds the default warning threshold, a warning pops up, reminding you to replace a new active carbon filter.

  • Page 58: Event View

    Operation 5.2.9 Event view View daily run log file. Fig. 44 Viewing log file 1. Press Event View (→ "Fig. 44-1"). 2. Select a log file you want to view (→ "Fig. 44-2"). 3. Press Open (→ "Fig. 44-3"), and the Event Viewer window pops up. 4.

  • Page 59: Reagents

    For the Cleaning reagent, the cycle number is recorded as 1 cycle after the whole Cleaning program is completed. Exchange date - Days since last change. It is displayed if the RMS mode is set to be Days. Expiry Date. It is displayed if the RMS mode is set to be Days. HistoCore PEARL...

  • Page 60: Groups

    Operation -- No column is displayed if the RMS mode is set to be Off. When the reagents are expired, the texts in the fields are highlighted with red. Fig. 46 Note After filling a bottle manually the bottle must be defined as Full. Editing reagent status L Make sure that no program is running.

  • Page 61: Stations

    • Stations 1 – 11 can only be assigned to specimen processing reagents (other than paraffin). • Station 12 can only be assigned to a cleaning solvent. • Station 13 can only be assigned to a cleaning ethanol. • Station 14 is the condensate bottle. HistoCore PEARL...

  • Page 62: Rms

    48-5"). 5.3.4 RMS The HistoCore PEARL has a Reagent Management System (RMS) that ensures optimized reagent consumption and the best possible tissue processing results. The Reagent Management System is controlled via warning threshold values that display the reagent replacement. For each reagent, a value of this type can be defined depending on the use.
  • Page 63 Changing the threshold value to more than recommonded, may impact the tissue processing quality. Threshold Value Reagent ReagentGroup Cassette Cycle Formalin Fixation Processing water Water Diluted ethanol Dehydrating, diluted 1000 Absolute ethanol Dehydrating, absolute 1000 Xylene Clearing 1000 Paraffin Paraffin 1000 Cleaning xylene Cleaning solvent Cleaning ethanol Cleaning ethanol HistoCore PEARL...
  • Page 64 Operation Setting the RMS Fig. 49 Editing the RMS L Make sure that no program is running. L Only Supervisor and Service user level can edit the RMS. 1. Press (→ "Fig. 49-1"). 2. Select a reagent (→ "Fig. 49-2"). 3.
  • Page 65 6. Press (→ "Fig. 50-7").  The new reagent is displayed on the Status window. Deleting a reagent L Make sure that no program is running. L Only Supervisor and Service user level can edit the RMS. HistoCore PEARL...

  • Page 66: Program

    At the Supervisor or Service level, and if no program is running, the menu Program is in edit mode. The customized programs can be added, deleted or edited by Supervisor. Note HistoCore PEARL is normally used in sequential mode, which means that the instrument uses one bottle after the other. Note The pre-installed processing programs and Cleaning program are preset and listed on the top of the user programs.

  • Page 67: Adding/Editing Programs

    L Only Supervisor and Service user level can create/edit/delete customized programs. 1. In the Program window (→ "Fig. 52") , select a program (→ "Fig. 52-1") as similar as possible to the program you wish to create. (This minimizes the number of editing steps). HistoCore PEARL...
  • Page 68 Operation 2. Press Copy (→ "Fig. 52-2") to copy the selected program. The Edit Program window pops up. The new program will have the same name as the program copied, however, the suffix "_cp" will be added to indicate the change. For example, if the "Biopsy" is copied, the new program is named as "Biopsy_cp".
  • Page 69 (→ P. 48 – 5.2.2 Regional Settings). 4. Press Save on the Edit Program Step window (→ "Fig. 54-7"). 5. To continue to edit other steps, repeat from Step 2 to Step 4. Otherwise, go to Step 6. HistoCore PEARL...
  • Page 70 Operation 6. Press Save on the Edit Program window (→ "Fig. 54-8"). Adding a new program step L Make sure that no program is running. L Make sure that the program has less than 14 steps. L Only Supervisor and Service user level can create/edit/delete a new program. Fig.
  • Page 71 3. After you confirm deleting the step, press Save (→ "Fig. 56-5"). Deleting a program L Make sure that no program is running. L Only Supervisor and Service user level can create/edit/delete a new program. HistoCore PEARL...

  • Page 72: Favorite Programs

    Operation Fig. 57 1. Select the program that is to be deleted (→ "Fig. 57-1") and press (→ "Fig. 57-2"). The Delete confirmation message box pops up. 2. Press "Yes" to delete the selected program. 5.4.2 Favorite programs Favorite programs are displayed on the Program List Panel on the dashboard. Up to 5 programs can be selected as favorite programs.

  • Page 73: Dashboard

    59-1"), paraffin baths status (→ "Fig. 59-2"), reagents status (→ "Fig. 59-3"), a list of favorite programs (→ "Fig. 59-4") and program running status, buttons of start/pause/abort (→ "Fig. 59-5"), and shows the seal/unseal status of retort lid. HistoCore PEARL...
  • Page 74 Operation Retort status Fig. 60 The retort is filled with the reagent group color that the reagent in the retort belongs to. The retort has the following status: • Empty (→ "Fig. 60-1") - The retort is empty and clean. • Partially filled (→...
  • Page 75 An animation shows the reagent going out of the retort and into the station. HistoCore PEARL...

  • Page 76: Reagent Handling

    Operation Program list panel Fig. 64 Program list panel is displayed when no program is running (→ "Fig. 64-1"). It is switched to the program running panel after a selected program starts (→ "Fig. 64-2"). If a program is selected, the program icon and name is highlighted (→...

  • Page 77: Filling/Refilling Paraffin

    There are two marks on the interior wall of the paraffin bath that display the maximum and minimum filling level when filled with paraffin. The level must be between these marks. The paraffin bath can be filled with paraffin pellets, paraffin blocks or liquid paraffin. HistoCore PEARL...
  • Page 78 Operation If the paraffin baths temperature is set to below 64 °C, or at 64 °C, the melting time is approximately 12 hours. If the paraffin baths temperature is set higher than 64 °C, the melting time takes longer, approximately 3 additional hours. For setting the paraffin baths temperature, see (→...

  • Page 79: Starting A Program

    Check that the reagent bottles and paraffin baths are correctly inserted. Caution Never leave a paraffin bath empty. The absence of the paraffin may cause incorrect heating to the paraffin bath. HistoCore PEARL...
  • Page 80 Operation Caution Make sure that the simulation of the reagent bottles on the display and the reagent bottles in the reagent cabinet are correctly matched. Caution Check that the retort sieve is correctly installed at the bottom of the retort, then make sure that the retort lid is sealed before a program starts.
  • Page 81 (→ "Fig. 67-3"). The program starts. The Dashboard is switched to the program running panel. Note After press ASAP, the Endtime field (→ "Fig. 67-1") is displayed as ASAP, and the button changes to Run. HistoCore PEARL...
  • Page 82 Operation Caution Monitor the instrument during Pre-checking and formalin soaking in the retort, in case any message pops Caution Do not open the retort lid or remove reagent bottles/paraffin oven cover/paraffin baths when a program is running, unless a system message indicates to do so. Doing so may cause damage to the instrument or trigger an error message.
  • Page 83 (→ "Fig. 68-8")/Abort Program button (→ "Fig. 69-10"). At the Standard user level, the Abort Abort button is only enabled during the first program step; at Supervisor/Service level, the Abort button is enabled at any time within the program. HistoCore PEARL...

  • Page 84: Ending A Program

    Operation • Current step (→ "Fig. 69-9") is highlighted with red. The duration column is showing only the soaking/ infiltration time. • Close button (→ "Fig. 69-11"). Press Close to close the program details window. Note The Step time (→ "Fig. 68-3") and the Remaining time (→...
  • Page 85 Duration Temperature P/V Formalin 0:15 Processing Water 0:02 Ethanol (70%) 0:05 Ethanol (80%) 0:05 Ethanol (95%) 0:05 Ethanol (100%) 0:05 Ethanol (100%) 0:05 Ethanol (100%) 0:10 Xylene 0:10 Xylene 0:10 Xylene 0:10 Paraffin 0:05 Paraffin 0:05 Paraffin 0:10 Fig. 70 HistoCore PEARL...
  • Page 86 Operation Note The Duration is the time of soaking/infiltration. The two pre-installed programs above cannot be edited or deleted, they can be copied. Version 1.3, Revision D...

  • Page 87: Cleaning And Maintenance

    Do not open the retort lid during the Dry step of the cleaning program. Caution Do not pull the reagent bottles when the cleaning program is running. It may cause damage to the instrument or trigger an error reporting. HistoCore PEARL...

  • Page 88: General Instrument Cleaning

    Baskets can be cleaned together in the retort. ONLY completely cleaned baskets can be used for tissue processing. After a maximum of 5 cleaning cycles, cleaning xylene and cleaning ethanol must be replaced. Neither drip trays nor other objects can be cleaned using the HistoCore PEARL Cleaning program! • After you touch the button, a message appears indicating that before the cleaning is started the retort must be empty and all specimens have to be removed.
  • Page 89 • Make sure that the air vent holes at the top front of the retort are not dirty. Cleaning the level sensor Note The level sensor must be cleaned of all residue (reagents and paraffin). Use a lint-free cloth to clean the level sensor. HistoCore PEARL...

  • Page 90: Weekly Cleaning And Maintenance

    Cleaning and maintenance Checking the bottom tray Caution Some of the reagents used in the HistoCore PEARL are hazardous to health. Therefore always wear chemical protective clothing, protective goggles, respirator, rubber gloves and all other necessary personal protective equipment, when handling them.

  • Page 91: Monthly Cleaning And Maintenance

    Regularly check the retort lid seal for damage. If the seal is damaged, it must be replaced without delay. The retort lid sealing must be inspected at least once a year. Contact Leica Biosystems Technical Service for the inspection/replacement service.

  • Page 92: Recommended Cleaning Reagents

    Cleaning and maintenance Caution Never clean reagent bottles in an automatic dishwasher. The reagent containers are NOT dishwasher-proof! Care of reagent and condensate bottle seals. • To ensure easy removal of the reagent and condensate bottles, lubricate the O-ring seals on the plug- in nozzles with the O-ring lubricant supplied.
  • Page 93 O-rings of the reagent bottles and check them for damage. Make sure that the shutter caps of the reagent bottles and the O-rings fit correctly and are tight. Check the paraffin oven cover seal. Clean the √ paraffin oven. Replace the active carbon filter. √ HistoCore PEARL...

  • Page 94: Troubleshooting

    Troubleshooting Troubleshooting Troubleshooting When a problem occurs with the HistoCore PEARL, take the following steps to diagnose the problem: 1. Read the error message(s). 2. Follow the instructions on the message. 3. Check the Run Log for any messages that indicate the nature of the failure. If the failure occurred during a program run, check the Run Log for an indication of whether the failure occurred during the fill, processing or drain cycles, and the station in use at the time.

  • Page 95: Malfunctions In The Program Sequence

    Open the retort lid and visually check the safe reagent level in the retort. If the safe reagent level is insufficient, close the retort lid and drain the reagent, then fill the safe reagent bottle to sufficient. Fig. 73 HistoCore PEARL...

  • Page 96: Crash Or Freeze Of Master Software

    7.3.2 Crash or freeze of master software Follow below steps to protect the specimens. 1. Take out specimens from the retort. 2. Restart the instrument. If the problem still exists, call Leica Biosystems Technical Service. Replacing a fuse Caution Before replacing a fuse, switch the instrument off and pull out the power plug.

  • Page 97: Ordering Information

    Funnel, 255 mm, D=250 mm 14047643631 Connector male 5-pole 14049351429 Extraction tube 14049544881 Silicone pad 14049350559 Active carbon filter 200X100mm 36mm 14049350629 Main control fuse 14049354395 Reagent bottle label kit Order No: 14049353389 Fig. 76 Paraffin scraper Order No: 14039353643 Fig. 77 HistoCore PEARL...
  • Page 98 Ordering information Maintenance kit Order No: 14049353391 Fig. 78 Funnel Order No: 14049355900 Fig. 79 Extraction tube Order No: 14049544881 Fig. 80 Version 1.3, Revision D...
  • Page 99 Ordering information Optional silicone plate kit Order No: 14049353396 Fig. 81 Active carbon filter Order No: 14049350629 Fig. 82 HistoCore PEARL...

  • Page 100: Warranty And Service

    Service information If you are in need of technical customer support or spare parts, please contact your Leica Biosystems representative or the Leica Biosystems dealer where you purchased the instrument. Please provide the following information: • Model name and serial number of the instrument.

  • Page 101: Decontamination Confirmation

    Decontamination Confirmation Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data.
  • Page 102 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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