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Leica BIOSYSTEMS SM2010 R Instructions For Use Manual

Sliding microtome
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Instructions for Use
Leica SM2010 R
Sliding Microtome
Instructions for Use
Leica SM2010 R, V 1.6 English – 10/2018
Order No.: 14 0508 80101 RevG
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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  • Page 1 Instructions for Use Leica SM2010 R Sliding Microtome Instructions for Use Leica SM2010 R, V 1.6 English – 10/2018 Order No.: 14 0508 80101 RevG Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 Instructions for Use are not to be considered as warranted characteristics of © Leica Biosystems Nussloch GmbH our products. Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 D –69226 Nussloch...

  • Page 4: Table Of Contents

    Table of contents Important Information ..........................3 Safety ................................. 4 Safety notes ..............................4 Warnings ............................... 4 Instrument Components and Specifications ..................7 Overview - instrument components ......................7 Technical data .............................. 8 Instrument Setup ............................ 10 Standard delivery ............................10 Site requirements ............................

  • Page 5: Important Information

    In vitro diagnostics (IVD) medical device Observe the Instructions for Use Order No. Serial number Qualification of personnel • The Leica SM2010 R may be operated by trained laboratory personnel only. Leica SM2010 R...

  • Page 6: Safety

    Safety Be sure to comply with the safety instructions and warnings provided in this chapter. Be sure to read these instructions, even if you are already familiar with the operation and use of other Leica products. Safety notes These Instructions for Use include important To maintain this condition and ensure safe opera- information related to the operating safety and tion, the user must observe all notes and warnings...
  • Page 7 • Do not use solvents that contain acetone or xylene! • Ensure that liquids do not enter the interior of the instrument during cleaning! • When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations! Leica SM2010 R...
  • Page 8 Safety Integrated safety devices The instrument is equipped with the following safety devices: • Knife guard (14) on the blade/knife Knife sledge holder. locked • Knife sledge locking knob (23) for the knife sledge. Knife sledge locking knob The knife sledge is locked in place us- Knife sledge ing the locking knob (23) that engages is movable...

  • Page 9: Instrument Components And Specifications

    Ergo handle for moving the knife sledge HN40 tensioning clamp Setscrew for orientation Adjusting knob of perpendicular to automatic feed cutting direction Knife sledge Removable section waste tray Magnetic knife sledge immobilizer Fig. 4 Leica SM2010 R...

  • Page 10: Technical Data

    Instrument components and specifications Technical data General Approvals: The instrument-specific marks are located on the name plate. Operating temperature range: 10 °C to +40 °C Relative humidity: max. 80% non-condensing Operating temperature range during storage: + 5 °C to +55 °C Storage humidity: <...
  • Page 11 Instrument Components and Specifications Instrument specifications • The Leica SM2010 R is a manually operated sliding microtome, designed as a low-maintenance tabletop instrument with roller-guided knife sledges and automatic section thickness feed. • Stable, torsion-free basic design with micrometer feed system in a closed housing, protected from ingress of paraffin waste.

  • Page 12: Instrument Setup

    Instrument Setup Standard delivery The Leica SM2010 R standard delivery includes: 1 Leica SM2010 R basic instrument ..........14 0508 42258 1 section waste tray ................ 14 0508 42328 1 tool set consisting of ..............14 0508 42983 1 Allen key size 6 ................14 0194 43634 1 Allen key with handle, size 4 ............

  • Page 13: Unpacking

    Fig. 5 Loosening the transport locking screw of the knife sledge The locking knob (23) secures the knife sledge during transport. In daily operation, it is also used to lock the knife sledge in place. Fig. 6 Leica SM2010 R...
  • Page 14 Instrument Setup Horizontal alignment For safe and accurate work, it is important that all instrument feet are in uniform contact with the installation surfaces. The microtome is horizontally aligned at the fac- tory. If a completely level or horizontal surface is not available at the installation site, the instrument must be realigned.

  • Page 15: Assembling The Knife Holder Sn

    • Insert the five countersunk screws (61. –61. into the bores and tighten them crosswise (in the sequence shown in Fig. 10) using an Allen key T25 (63). 61.1 61.3 61.5 61.4 61.2 Fig. 10 Leica SM2010 R...
  • Page 16 Instrument Setup Assembling the knife holder SN (continued) Fastening the knife holder The knife holder SN (67) has two bores (64) and thus two different mount- ing positions for different sectioning requirements. 64.1 66.1 64.2 • Press the clamping lever (66) of the knife holder (67) downwards and screw the bottom of the thread (66.
  • Page 17 • On the intermediate plate (60) there is an index mark (68.2) which serves as a point of refer- ence for the scale division when adjusting the declination. • For fixation, tighten the clamping lever (66) 68.1 firmly in the required position. Fig. 14 68.2 Leica SM2010 R...

  • Page 18: Assembling The Blade Holder Se

    Instrument Setup Assembling the blade holder SE The blade holder SE is designed for conventional disposable blades from all common manufactur- ers. It is available in two models: one for low-profile blades and one for high-profile blades. The blade holder SE has a lateral movement, so that the entire width of the blade can be used.
  • Page 19 Allen key T25 (63). • To clamp the blade holder on the grooved plate, tighten the screw (52) on the back of the blade holder base (17) using a size 6 Allen key (Fig. 19). Fig. 18 Leica SM2010 R...
  • Page 20 Instrument Setup Assembling the blade holder SE Inclination (declination) (continued) of the blade holder SE • Unscrew the Allen screw (52) at the back of the blade-holder base using an Allen key SW 6. • Turn the blade holder to the required position. 55.1 •...
  • Page 21 Enlarged detail showing a clearance angle setting of 4°. 43.2 The recommended clearance angle set- ting for the blade holder is approx. 4°. 43.1 • Hold down the blade holder in this position and retighten the screw (15) for clamping. Fig. 21 Leica SM2010 R...
  • Page 22 Instrument Setup Inserting the universal cassette clamp The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place. You can use the quick clamping system (29) to hold all the available accessory specimen clamps (for more information, see Chapter 7 "Optional Accessories").

  • Page 23: Operation

    Neutral: Turning the coarse driving wheel does not result in a feed motion. The coarse driving wheel does not turn as well during sectioning. Fig. 23 Leica SM2010 R...

  • Page 24: Manual Feed

    Operation 5.1.3 Manual feed The lever (35) for the manual feed is at the front right of the instrument. • Each time the lever is pushed or pulled, this causes a feed motion for trimming or section- ing by the value set on the scaled adjusting knob (33).

  • Page 25: Directional Fixture For Specimen Clamps

    For better display of the zero position, each set- screw (30, 31) has a red mark (32). When both marks are visible and both setscrews are in zero position at the same time (notch point!), Fig. 26 the specimen orientation is in zero position (0°). Leica SM2010 R...

  • Page 26: Clamping The Specimen In The Universal Cassette Clamp (Ucc)

    Operation Clamping the specimen in the universal cassette clamp (UCC) Caution! Always clamp the specimen block BE- FORE clamping the knife. Lock the knife sledge and cover the knife edge / blade edge with the knife guard prior to any manipulation of knife/ blade or specimen, prior to changing the specimen block and during all work breaks!

  • Page 27: Clamping A Disposable Blade

    • Loosen the clamping lever (46) of the pressure plate (48). • Push the knife guard (14) towards the left while holding down the black push button (51). This pushes out the blade far enough sideways that it can be removed easily. Fig. 29 Leica SM2010 R...

  • Page 28: Replacing The Pressure Plate

    Operation Replacing the pressure plate The blade holder SE can be converted from use of narrow blades to use of wide blades, and vice versa. To do this, both the pressure plate (48) and the matching insertion aid (57) have to be replaced. To replace them, proceed as follows: •...

  • Page 29: Inserting The Knife

    • Take the knife out of the knife case and insert it carefully. • Tighten the two clamping screws (72) in alternation until both are secure and cover the knife with the knife guard. Proceed the same way to clamp a blade rail. Fig. 34 Leica SM2010 R...

  • Page 30: Sectioning

    Operation Sectioning Cutting into the specimen (trimming) For trimming, the specimen feed can be dis- engaged either by turning the coarse driving wheel (36) or by operating the manual feed lever (35). • Hold the knife sledge (11) at the grip (12) and place the sledge behind the specimen.

  • Page 31: Changing The Specimen Or Interrupting Sectioning

    • Remove the specimen from the specimen clamp. • Push all section debris into the section waste tray and empty the tray. • Clean the instrument (see Chapter 8.1). Leica SM2010 R...

  • Page 32: Cleaning And Maintenance

    Cleaning and maintenance Cleaning the instrument • Always remove the knife or blade before detaching the knife/ blade holder from the instrument! • Always put the knives back into the knife case when not in use! • Never place a knife anywhere with the cutting edge facing up- wards and never try to catch a falling knife! •...
  • Page 33 The intervals depend on how heavily the instrument is used. • Enter into a service contract at the latest at the end of the warranty period. For more information, please contact your local Leica technical service center. Leica SM2010 R...

  • Page 34: Optional Accessories

    Optional accessories Ordering information Supermega cassette clamp with adapter, silver ..............14 0508 42634 Universal cassette clamp with adapter, silver ................. 14 0508 42635 HN40 clamp with adapter, silver ....................14 0508 42637 Standard specimen clamp with adapter, silver ................ 14 0508 42632 Dry ice tub with adapter, silver....................
  • Page 35 Order No..........14 0508 42635 Fig. 37 HN40 tensioning clamp, with adapter, silver for mounting in the quick change system for specimen imaging Maximum specimen size: 59 x 45 mm (L x W) Order No..........14 0508 42637 Fig. 38 Leica SM2010 R...
  • Page 36 Optional accessories Standard specimen clamp, with adapter, silver for mounting in the quick change system for specimen imaging Maximum specimen size: 79 x 60 mm (L x W) Order No..........14 0508 42632 Fig. 39 Dry ice tub with adapter, silver Order No.
  • Page 37 Order No..........14 0508 43693 Pressure plate kit BB, assembly consisting of: - 1 pressure plate BB ....14 0508 43691 - 1 blade insertion aid BB ..... 14 0508 43687 Fig. 43 Order No..........14 0508 43694 Leica SM2010 R...
  • Page 38 Optional accessories Knife holder SN, assembly for resharpenable knives or blade rails. Blade holder declination with scale for reproducible ad- justment up to 45°. 2 clamping screws for quickly and securely clamping the cutting tool. Safe lateral displacement of the cutting tool for using the entire blade edge.
  • Page 39 Fig. 49 Knife, 22 cm Profile C, steel for paraffin and cryosections; Note: including knife case 14 0213 11141 Order No..........14 0216 07116 Fig. 50 Ball handle, assembly Order No..........14 0508 42565 Fig. 51 Leica SM2010 R...
  • Page 40 Optional accessories Leica low-profile disposable blades - Type 819 Length 80 mm, height 8 mm 1 package of 50 pieces Order No..........14 0358 38925 Fig. 52 Leica high-profile disposable blades - Type 818 Length 80 mm, height 14 mm 1 package of 50 pieces Order No.

  • Page 41: Troubleshooting

    • Too great of a sectioning force • Adjust the sectioning speed and/or 2. High blade consumption was applied. section thickness when trimming. Select a smaller section thickness, move knife sledge more slowly. Leica SM2010 R...

  • Page 42: Warranty And Service

    Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is fault- less and complies with all technical specifications and/or characteristics warranted.

  • Page 43: Decontamination Confirmation

    Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decon- tamination confirmation on our website www.LeicaBiosystems.com within the product menu.
  • Page 44 Notes Instructions for Use V 1.6 RevG – 10/2018...
  • Page 46 Leica Biosystems Nussloch GmbH Heidelberger Str. 17–19 D– 69226 Nussloch Phone: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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