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Leica BIOSYSTEMS Aperio AT2 Console User Manual

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The Pathology Company
Aperio AT2 Console
User's Guide
MAN-0251, Revision D | 13 August 2015

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Summary of Contents for Leica BIOSYSTEMS Aperio AT2 Console

  • Page 1 The Pathology Company Aperio AT2 Console User’s Guide MAN-0251, Revision D | 13 August 2015...
  • Page 2 Ì This manual is not a substitute for the detailed operator training provided by Leica Biosystems Imaging or for other advanced instruction. Leica Biosystems Imaging Field Representatives should be contacted immediately for assistance in the event of any instrument malfunction. Installation of hardware should only be performed by a certified Leica Biosystems Imaging Service Engineer.
  • Page 3 Tel: 0080052700527 (toll free) In-country Tel: +31 70 413 21 00 Danmark: Email: support.nl@leicabiosystems.com Tel: 0080052700527 (toll free) In-country Tel: +45 44 54 01 01 Email: support.dk@leicabiosystems.com Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 4 Tel: 0080052700527 (toll free) In-country Tel: +44 1908 577 650 Email: support.uk@leicabiosystems.com USA: Tel: +1 866 478 3999 (toll free) Direct International Tel: +1 760 539 1150 Email: TechServices@leicabiosystems.com Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 5: Table Of Contents

    Using the Batch View ........................25 Viewing a Slide’s Snapshot ......................26 Viewing a Slide After the Scan ......................27 Viewing the Slide Label ......................27 Rescanning Failed Slides ......................27 Marking Slides for Rescanning .......................28 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 6 Manually Focusing Focus Points ......................52 Manually Focusing the Tissue ......................55 The Video Monitor Window Controls ....................56 Z-Stack Scanning ......................57 Basic Z-Stack Settings ........................58 Advanced Z-Stack Settings .......................58 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 7 Slide Types Override Console Options ....................73 Properties .............................73 Controller Properties ........................73 Image Filtering ..........................76 Alignment Threshold ........................77 Compression Types .........................77 Slide Types ...........................78 Slide Settings ..........................79 Index ............................. 80 Symbols..........................83 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 8: Getting Started

    Minimum monitor resolution: 1600 x 1020 Ì The Console software is generally installed on the workstation connected to the scanner at the Leica Biosystems Imaging factory. If you have questions about how to obtain this software to install it on additional workstations to access the scanner over the network, please contact your service representative.

  • Page 9: Selecting The Scan Data Group

    If you are not sure what user name and password to use, contact your system administrator for help. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 10: Quick Tour Of The Console

    Also on the information banner are icons that select different Console modes, such as batch view and single slide view. Note that the currently selected icon is displayed with a darker border. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 11: Working Area

    Selects all slides that are in the scan batch. Selects all slides that failed to scan. Selects all slides in all racks. Removes all Slide Viewed indicators, Excludes all selected slides from the scan batch. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 12: Console Slide View

    If multiple slides are selected, clicking this icon takes you to the detailed view of the first selected slide. You can use the arrow symbols below the slide display to navigate between all of the selected slides. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 13: The Console Batch View

    To return to the previous view, click the back-arrow: The Console Batch View The batch view provides another way of looking at the slides in a scan batch. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 14: Judging Scan Quality

    After the slide is scanned, the rack view gives you an indication of the success of the scan. The slide tray or rack graphic will display a color for each slide processed. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 15: Scan Quality Tips

    Is uniformly in focus with no blurry areas Ì Is not too dark or too faint Ì For help in correcting possible scanning problems, refer to “Appendix A: Troubleshooting” on page 69. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 16: Glossary

    The hardware component that holds the slide to be scanned and moves under the objective for scanning. Z-stack Collection of multiple images of a slide at different focal depths. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 17: Scanning A Batch Of Slides

    Any slides in the slide positions shown in the rack display that have a white color are not included in the current scan batch nor are they selected. A white status color means that the status of the slide in that position is not known—there may be a Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 18: Replacing A Rack

    To replace an AutoLoader rack, eject the rack—this tells the scanner to move the rack to the access position. Then remove the rack from the scanner, re-load it or replace it with another loaded rack and place it back in the scanner. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 19: Including Slides In A Scan Batch

    If you want to unselect that slide, hold down the Control key and click the slide again. An unselected slide that is included in a scan batch looks like this: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 20 Click the top of the rack to select all slides in the rack. scan batch Then right-click on the group of selected slides and select Exclude selected slides on the context menu. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 21: Selecting/Unselecting Slides And Racks

    However, note that the scanner will skip scanning slides that show a green status color (because they have already been successfully scanned) and slides that show orange or red status colors (because they need to be reviewed and adjusted before they can be rescanned). Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 22: Setting Scan Magnification

    Optionally set a name for all selected slides (this text will be used as the basis for the file names for these slide images). Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 23 Put your cursor over it in rack view or batch view. You see the View Slide icon. In rack view: Or in batch view: Click this icon to see this slide in the slide view window. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 24 Scan the slide again and other reviewed slides in the scan batch by clicking the Scan button. Ì To return to the previous view, click the back arrow: Ì For more information on tehse options, “Chapter 4: Using Snapshots” on page 41. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 25: Using The Batch View

    To select all slides on this window, click the Select all slides icon: Ì To unselect all slides on this window, click the Deselect all slides icon: Ì To return to the previous view, click the back arrow: Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 26: Viewing A Slide's Snapshot

    Console session. To clear all Slide Viewed indicators from the rack view, click the Clear slide viewed indicators button at the bottom of the rack view: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 27: Viewing A Slide After The Scan

    The Console makes it very easy to rescan just those slides that the scanner could not scan (indicated with a red status color) by using the Select failed slides icon on the Console command center: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 28: Marking Slides For Rescanning

    By the time all snapshots have been taken, you have only a few remaining snapshots to review. When finished with all snapshot review, click the Scan button to start scanning the slides. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 29: Scanning Multiple Groups Of Slides

    You can also use the Slide Settings drop-down list to select an entire set of parameters for a group of slides. (Slide settings are defined and optionally saved when using snapshots or during a manual scan. For details, see “Saving Slide Settings” on page 45.) Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 30: Manually Scanning A Slide On The Stage

    You can use the slider under the macro image to zoom into the image: Use the zoom slider to zoom into the image—click the blue dot to zoom into the entire macro image, the red dot to zoom Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 31: Set The Scan Magnification

    Or, you can manually change the area of the slide that will be scanned: Click the green pin on the scan area box: and the cursor changes to a pen so you can draw a new scan area: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 32: Verify The Calibration Point Position

    If the characteristics of a specific slide makes it difficult for the scanner to create a calibration image, you can Ì use a previously saved default calibration image for this slide. To use the default calibration image, click the Use default calibration image icon: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 33: Set Focus Points

    You can either test the focus points before the scan by clicking the Auto Focus icon: Or you can let the scanner focus the focus points during the scan by simply scanning.Focus points turn green as they are focused. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 34: Z-Stack Scanning

    As the scan proceeds, you can see the stripes scanned as the focus points disappear (note the stripe numbers at the bottom of the image). The blue sphere represents the objective and shows the progress of the scan: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 35 You also see status information in the information box at the top of the window: If you need to stop the scan, click the Stop button (which replaces the Scan button when the scanner is busy): Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 36: View The Scanned Image

    To view the scanned image, click the magnifying glass in the scan area to open the image in the Console viewer: If you are in rack view, you can view the slide on the stage by clicking the Slide View icon at the top of the page: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 37: View The Slide Label

    Performing a manual macro focus Ì Auto focusing focus points before the scan Ì Focusing a focus point Ì Any action that uses the Video Monitor window Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 38: For More Information

    For information on making calibration images, see “Chapter 7: Calibration” on page 60. Ì For information on z-stack scanning, see “Chapter 6: Z-Stack Scanning” on page 57. Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 39: Reference Guide

    “Chapter 8: Console Tools” on page 63 The following appendices are also included: “Appendix A: Troubleshooting” on page 69 Ì “Appendix B: System Properties” on page 72 Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 40 Reference Guide Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 41: Using Snapshots

    After reviewing and adjusting all the snapshots, start scanning those slides. Taking Snapshots To take snapshots, select one or more slides in rack view: And click the Snapshot button: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 42: Reviewing Snapshots

    The slide view window appears: Features of this window include: Viewing Controls – By using the slider control you can zoom in and out of the slide image: Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 43 Selecting Slide Settings and Slide Types – You can apply a slide type or slide setting to this slide by using Ì the Slide Type and Slide Settings drop-down lists. (See “Saving Slide Settings” on page 45 for information on creating your own slide settings.) Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 44: Setting Scan Magnification

    To clear all Slide Viewed indicators from the rack view, click the Clear slide viewed indicators button at the bottom of the rack view: Setting Scan Magnification Click the magnification buttons to select the scan magnification you want to use for this slide: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 45: Saving Slide Settings

    If you want to delete any of the slide settings you previously saved, click the Slide Settings drop-down list and click the red X next to the setting you want to delete. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 46: Set The Scan Area

    Note that you can use the same techniques to redraw, adjust, or move the maximum scan area (shown in red), however it is not recommended to change the maximum scan area except in rare circumstances. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 47: Verify The Calibration Point Position

    If the characteristics of a specific slide makes it difficult for the scanner to create a calibration image, you can Ì use a previously saved default calibration image for this slide. To use the default calibration image, click the Use default calibration image icon: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 48: Set Focus Points

    The starting slide has an arrow next to it in the rack view. The scan will skip red and orange slides (which need to be reviewed and adjusted before they can be scanned again) and green slides (which have already been successfully scanned). Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 49: Viewing A Slide's Snapshot

    Viewing a Slide After the Scan After opening the scanned slide in slide view (see the section above), click the magnifying glass to view the slide in the Console viewer. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 50: Working With Focus Points

    (as in Levels 2, 3, and 4), and perform a macro focus to focus the tissue (as discussed in “Manually Entire Slide Focusing the Tissue” on page 55. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 51: Automatically Placing Focus Points

    The new focus point appears as a yellow dot. Or, right-click on the position where you want to add a focus point and select Add Focus Point Here from the context menu. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 52: Automatically Focusing The Focus Points

    Manual Focus check box on the Video Monitor window (see below). Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 53 As an alternative to moving the slide, you can enter a specific value by clicking on the number at the bottom of the slider, where you can use the controls to increment or decrement the value or type in a new value: Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 54 Video Monitor window. You see that the focus point on the slide image is now green, indicating it has been focused. For information on additional features of the Video Monitor window, see “The Video Monitor Window Controls” on page Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 55: Manually Focusing The Tissue

    When the tissue is in focus, close the Video Monitor window. For information on additional features of the Video Monitor window, see the next section. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 56: The Video Monitor Window Controls

    (so that the focus values you set manually are not overridden) select the Manual Focus check box. Always on Top Check Box – To keep the Video Monitor window in front of any other window, select this check Ì box. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 57: Z-Stack Scanning

    Now adjust the basic or, optionally, the advanced settings as discussed in the sections below. To apply the settings to the slide you are viewing in the slide view window, click Apply. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 58: Basic Z-Stack Settings

    It may take some experimentation to determine the optimal placement of the best focus layer to achieve the best results.. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 59: Saving Z-Stack Settings As A Slide Setting

    On the Save Slide Settings window, select the Z-Stack Scanning check box. Type a name for the slide setting. Click Save. Viewing Z-Stacks For information on viewing the 3-dimensional z-stack image, refer to the ImageScope User’s Guide. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 60: Calibration

    Place the slide on the stage with the coverslip up (see the user guide for your scanner for instructions on the orientation of label of the slide on the stage). Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 61 If you want to save this image to use as a default calibration image, click the Save as Default icon on the Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 62: Using A Default Calibration Image

    The scanner calibrates itself each time you scan a slide and sends the calibration information to eSlide Manager where you can access a complete report of calibrations. See the eSlide Manager Administrator’s Guide for details. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 63: Console Tools

    The System Properties window contains settings for configuring the scanner. To see the System Properties window, go to the Tools menu and select System Properties. Details on using the System Properties window are contained in “Appendix B: System Properties” on page 72. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 64: Tissue Finder Configuration (Creating Slide Types)

    You must be in slide view to open the Slide Type Settings window. For information on saving and working with slide type settings, see “Saving, Modifying, Testing, and Deleting Slide Types” on page 67. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 65 Typical offset values are between -0.3 to +0.3 microns, although they may be outside of that range. Best scans are obtained by determining the offset by trial and error. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 66: Setting The Maximum Focus Point Area

    Select the Show/Modify Max Focus Point Area check box on the Slide Type Settings window. A gold rectangle appears on the slide view and the scan area rectangle is hidden. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 67: Tips On Using The Maximum Focus Point Area Rectangle

    If you want to replace a non-factory slide type setting, type its name and you will be asked to confirm that you want to overwrite the slide type setting. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 68 If a slide type setting has a red X next to it, click the X to delete the setting. (If a slide type does not have red X next to it, it is a factory slide type setting and cannot be deleted.) Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 69: Troubleshooting

    Is there dust or debris on the coverslip or bottom surface? Clean the slide. Are there air pockets under the coverslip? Perform a manual scan. Is there excess adhesive around edges of the coverslip? Clean or perform manual scan. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 70 You can set TempImageLocation to the correct directory by using the scanner Properties command of the Tools menu. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 71: Setting Line Camera Gains

    When you see this error window, for additional information click on the Scanner Log radio button to see a log of status and error conditions. This information may be requested by Technical Support if they are helping you troubleshoot an issue. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 72: System Properties

    Slide Type check box allows you to change a property even if it is in the slide type applied. Note that this option allows you to overwrite just the property in the slide type that you are changing, but does not disable any other property in the slide type. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 73: Slide Types Override Console Options

    AlignThreshold See “Alignment Threshold” on page 77. AutoLoader Set to True if AutoLoader is present; otherwise, False. BlueGain This parameter is the Basler line-camera blue gain setting. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 74 (“Ask”) or 2) continue the scan and save it locally without asking the user (“Continue”). ExerciseCount Determines the number of times the nanomotor stages will perform an exercise. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 75 Used by the tissue finder routines to select tissue and control the number of focus points placed on tissue. MacroWhiteSigmaThreshold Used by the tissue finder routines to select tissue and control the number of focus points placed on tissue. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 76: Image Filtering

    The unsharp mask filters listed above have different standard deviation values from .6 (Unsharpmask 6) to 1.0 (Unsharpmask 10). The higher the standard deviation, the more sharpening the filter performs. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 77: Alignment Threshold

    .JP2 by Kakadu Image Library. You may specify the target image quality using CompressionQuality (20-90). Output is a JP2 file. Compression ratios are in the range 20:1-25:1. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 78: Slide Types

    DoGainAdjust=True Ì RedGainAdjust=100 Ì GreenGainAdjust=100 Ì BlueGainAdjust=100 Ì Gain Adjust Medium For very dense slides. Sets the following properties: DoGainAdjust=True Ì RedGainAdjust=200 Ì GreenGainAdjust=200 Ì BlueGainAdjust=200 Ì Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 79: Slide Settings

    Choosing a slide setting from the Slide Settings drop-down box applies those settings to all slides on the stage or racks. See “Reviewing Snapshots” on page 42 for more on slide settings. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 80: Index

    50 Console manually focusing 52 batch view 13 moving 51 connecting to the scanner 9 placing 33 quick tour 10 setting 48 rack view 10 scanning 48 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 81 55 regulatory compliance, calibration reports 62 rescanning 28 troubleshooting 69 rotating slide label 37 uncompressed files 78 unsharp mask filters 76 saving images locally 9 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 82 56 viewing scanned image 36 viewing slide label 27, 37 z-stack scanning advanced options 58 basic options 58 best focus layer 57 slide settings 59 Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...

  • Page 83: Symbols

    La lampe UV dans un triangle équilatéral vise à avertir l’utilisateur de la présence de rayonnement UV dans le boîtier du produit qui peut être d’une amplitude suffisante pour constituer un risque pour l’utilisateur. Aperio AT2 Console User’s Guide, Revision D © Leica Biosystems Imaging, Inc. 2015...
  • Page 84 www.LeicaBiosystems.com/ePathology...

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