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Leica ST 5010 Autostainer XL Manual
Leica ST 5010 Autostainer XL Manual

Leica ST 5010 Autostainer XL Manual

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Leica ST 5010 Autostainer XL
Histolab IMG manual
Staining protocols:
Program 1: Hematoxylin & Eosin (Default, do not edit!)
Program 2: Deparaffinization and rehydration (Default, do not edit!)
Program 3: Dehydration (Default, do not edit!)
Program 4: Hematoxylin & Eosin (Editable)
Program 5: Hematoxylin & Eosin (Editable)
Program 6: Hematoxylin & Eosin (Editable)
Before start of staining:
Open the Autostainer cover and remove all covers from the containers. Check all solutions in the
containers. If you are not satisfied with the solution inside a container, you can replace it with a
fresh one or only add a sufficient volume. Always place the same container with the same solution in
the same position. Never mix containers! Discard solutions only into waste inside hood.
Staining:
1. Switch on Autostainer on right side panel.
2. Press F1 (Stain), Machine starts initializing process.
3. Select staining program with arrows (UP/DOWN).
4. Insert the slides in the staining rack into the LOAD position and close.
5. Press the LOAD button.
6. The protocol starts. You can pause or terminate the protocol by pressing F4 (Pause).
7. When the protocol finishes, the machine will start beeping.
8. Press the EXIT button, now you can open Exit container.
After staining:
Open the Autostainer cover and place all covers to containers. If you notice any dirty parts around
the containers inside the Autostainer, clean them with paper towel. Close Autostainer cover and
switch off machine on right side panel.

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Summary of Contents for Leica ST 5010 Autostainer XL

  • Page 1 Leica ST 5010 Autostainer XL Histolab IMG manual Staining protocols: Program 1: Hematoxylin & Eosin (Default, do not edit!) Program 2: Deparaffinization and rehydration (Default, do not edit!) Program 3: Dehydration (Default, do not edit!) Program 4: Hematoxylin & Eosin (Editable) Program 5: Hematoxylin &...
  • Page 2 Editing programs 4, 5, 6 1. Press F2 (Edit). 2. Select program you want to edit with arrows (Up/Down) 3. After finding program press F2 (Edit) 4. With arrows Right/Left you select editing step/station/time/Exact 5. With arrows Up/Down you select step number (1, 2, 3,….), Station (Oven, Station 1, 2, 3,..Wash1,..), Time (00:01 –...
  • Page 3 Program 1: Hematoxylin & Eosin (Default, do not edit!) Step Solution Time Station Step 1 Heating Station 15 min Oven (55 °C) Step 2 Xylene I 5 min Station 1 Step 3 Xylene II 3 min Station 2 Step 4 EtOH 96% 3 min Station 3...
  • Page 4 Program 2: Deparaffinization and rehydration (Default, do not edit!) Step Solution Time Station Step 1 Heating Station 15 min Oven (55 °C) Step 2 Xylene I 5 min Station 1 Step 3 Xylene II 3 min Station 2 Step 4 EtOH 96% 3 min Station 3...
  • Page 5 Program 4, 5, 6: Hematoxylin & Eosin (Editable) Step Solution Time Station Step 1 Heating Station 15 min Oven (55 °C) Step 2 Xylene I 5 min Station 1 Step 3 Xylene II 3 min Station 2 Step 4 EtOH 96% 3 min Station 3 Step 5...