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Summary of Contents for Biosan RTS-1
- Page 2 If you have any feedback on our products or services, we would like to hear from you. Please send all feedback to: Manufacturer: SIA Biosan Ratsupites iela 7 k-2, Riga, LV-1067, Latvia Phone: +371 674 261 37 www.biosan.lv Marketing: sales@biosan.lv Service: service@biosan.lv RTS-1, RTS-1C...
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Page 3: Table Of Contents
1. About this edition of user instructions 1.1. The current edition of the user instructions applies to the following models: Model and name Version RTS-1, personal bioreactor V.3AW RTS-1C, personal bioreactor V.4A01, V.5A01 1.2. Edition 3.-5.01 – May of 2023. -
Page 4: Safety Precautions
• If liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician. • Do not operate the unit in premises where condensation can form. Operating conditions of the unit are defined in the Specifications section. RTS-1, RTS-1C... - Page 5 2.5. During operation • Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact manufacturer for possible operation of the unit in specific atmospheres. • Do not operate the unit if it is faulty or has been installed incorrectly. •...
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Page 6: General Information
3. General Information Models RTS-1 & RTS-1C are personal bioreactors which utilize patented Reverse- ® Spin technology that applies non-invasive, mechanically driven, low energy consumption, in- novative type of agitation. In it, the cell suspension is mixed by rotating a single-use falcon bioreactor tube around its axis with a change of direction of rotation motion, resulting in highly efficient mixing and oxygenation for aerobic cultivation. - Page 7 Software possibilities: • Remote tracking and control of fermentation process. • Real-time registration of cell growth kinetics or particle suspension aggregation/ dis- aggregation processes. • User graphs, including 3D graphs. • Pause. • Saving and loading result data. • PDF and Excel spreadsheet reports. •...
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Page 8: Getting Started
Examine the unit carefully for any damage incurred during transit. The war- ranty does not cover in-transit damage. 4.2. Complete set. The unit set includes: RTS-1 / RTS-1C, Personal Bioreactor ................ 1 pce Lid ..........................1 pce ®... - Page 9 4.3. Setup. Place the unit on even, horizontal working surface; Connect the external power supply to the socket (fig. 1/1) on the rear side of the unit; Switch on the computer if it was turned off; Connect the USB data cable to the unit (fig. 1/2) and to the personal computer; Insert the USB disk drive in the personal computer and install the software following the software installation procedure described in software installation manual.
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Page 10: Calibration
Multiply the dilution factor values to get the OD of the samples. Continue to software manual page 29. 5.3.2. RTS-1 / RTS-1C can be calibrated to detect scattered light of any possible cell with any possible shape and size, but due to difference of light scattering in various cell suspensions, we cannot guarantee the stated measurement range in all conditions. - Page 11 5.4. Calibration verification for hardware versions 8 and earlier. The device is hardware calibrated at the factory for specific microorganism size of 0.4-0.8 x 1-3 μm and a cell volume of approximately 0.5-5.0 μm for operation with 50 ml tube at temperature range from +4°C to +70°C and saves calibration data when being switched off.
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Page 12: Operation
Operation stop will not stop the heating process. To stop heating process set temperature has to be decreased manually until “off” indication appears (fig. 2/5). 6.6. After finishing the operation, switch OFF the unit with the Power switch (fig. 1/3). 6.7. Disconnect external power supply from electric circuit. RTS-1, RTS-1C... -
Page 13: Recommended Methods For Microorganism Cultivation
37° C, 30 ml, 10 min MF 7.4. It is possible for the end-user to contact the manufacturer for advising or suggesting a required microorganism or strain to be tested. Please contact Biosan at the following e-mail address: service@biosan.lv. Edition 3.-5.01... -
Page 14: Recommendations For Creating Personal Settings For Cultivation Of Microorganisms. Points To Consider
(see fig. 5. and fig. 6.). 8.2.1. Legend of experiment (fig. 5.): Personal Bioreactor RTS-1 / RTS-1C was used with 850 nm LED, volume of LB media in 50 ml Falcon tube was 15 ml, Reverse Spin Interval 1, 2, 4, 8,... -
Page 15: Aeration And Types Of Recommended Tubes
Figure 5. Influence of Interval of Reverse Spinning on the Growth kinetics (ΔOD /Δt) vs Time λ=850nm of fermentation (h). Figure 6. Influence of Interval of Reverse Spinning on the Growth kinetics (ΔOD /Δt) vs Time λ=850nm of fermentation (h). 8.3. -
Page 16: Factory Calibration Particle Size And Calibration Coefficients 600Nm/850Nm
λ = 850 nm λ = 600 nm and volume. Therefore, the coefficient will be different for other cell size and volume. The de- vice can be calibrated at desired reference wavelength to meet the needs of the user. RTS-1, RTS-1C... -
Page 17: Temperature Control
9. Temperature control 9.1. Rotation intensity influence on temperature accuracy of the sample Rotation intensity influences air transfer introduced to the tube from the outside environment. Table 1. Temperature differences (Δ t°C) between tube sample and set thermoblock temperature, depending on aerobe and microaerophilic rotation intensities at ambient temperature 23 °C ±... -
Page 18: Cold Room And Environmental Chamber Influence On Temperature Accuracy Of The Sample
Temperature measurements of the sample and corrections at maximum and minimum temperature ranges or 4°C-10°C and 60°C-70°C must be performed by the user. If any addi- tional questions appear, please contact Biosan support for assistance by service@biosan.lv.. 9.4. Change of optical characteristics of the tube depending on temperature When temperature of the plastic material is changing, i.e. -
Page 19: Specification
10. Specification 10.1. Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice. 10.2. Measurement specifications RTS-1 RTS-1C Light source Wavelength (λ), nm 850 ± 15 E.coli BL21 Factory calibration... -
Page 20: Ordering Information
AU plug (type I, 230 V~, 50/60 Hz) BS-010160-A03 US / JP plug (type A, 100–120 V~, 50/60 Hz) BS-010160-A02 11.2. To inquire about or order the optional accessories, contact Biosan or your local Biosan representative. 11.2.1. Optional accessories: Description Catalogue number USB 2.0 Hub 10 ×... -
Page 21: Maintenance
12.1. Service and maintenance. 12.1.1. If the unit is disabled (e.g., no rotor movement, heating or PC connection) or requires maintenance, disconnect the unit from the mains and contact Biosan or your local Biosan rep- resentative. 12.1.2. All maintenance and repair operations (except listed below) must be performed only by qualified and specially trained personnel. -
Page 22: Warranty And Claims
Technical support section on our website at the link below to obtain the claim form. 14.5. Extended warranty. For RTS-1 & RTS-1C, the Smart class models, extended warranty is a paid service. Contact your local Biosan representative or our service department through the Technical support section on our website at the link below. -
Page 23: Device Calibration For Maintenance
15.9. Device is now calibrated and will make one verification measurement. 16. EU Declaration of Conformity 16.1. Personal bioreactors RTS-1 and RTS-1C are in conformity with the following relevant Union legislations: LVS EN 61010-1:2011 Safety requirements for electrical equipment for me- asurement, control, and laboratory use. - Page 24 Biosan SIA Ratsupites 7 k-2, Riga, LV-1067, Latvia Phone: +371 67426137 Fax: +371 67428101 Edition 3.-5.01 – May of 2023 https://biosan.lv...
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