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Biosan RTS-1 Operating Manual

Personal bioreactor
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Summary of Contents for Biosan RTS-1

  • Page 3: Table Of Contents

    Warranty and Claims ....................16 Device calibration for maintenance ................ 17 EU Declaration of Conformity ................. 18 About this edition of the manual The manual applies to the following versions and models of personal bioreactors:  RTS-1 version V.3AW  RTS-1C version V.4A01...

  • Page 4: Safety Precautions

    Safety Precautions The following symbol means: Caution! Make sure you have fully read and understood the present Manual before using the equipment. Please pay special attention to sections marked by this symbol. GENERAL SAFETY  Use only as specified in the operating manual provided. ...

  • Page 5: General Information

    General Information Models RTS-1 & RTS-1C are personal bioreactors which utilize patented Reverse- ® Spin technology that applies non-invasive, mechanically driven, low energy consumption, innovative type of agitation. In it, the cell suspension is mixed by rotating a single-use falcon bioreactor tube around its axis with a change of direction of rotation motion, resulting in highly efficient mixing and oxygenation for aerobic cultivation.

  • Page 6: Getting Started

    Examine the unit carefully for any damage incurred during transit. The warranty does not cover in-transit damage. 4.2. Complete set. The unit set includes: RTS-1 / RTS-1C, Personal Bioreactor............... 1 pce Lid ........................1 pce ® Bioreactor vessels TPP TubeSpin Bioreactor 50ml ........

  • Page 7: Calibration

    4.5. Due to the specificity of mould type manufacturing of centrifugal falcon tubes, the helical structure of the screw caps screw thread can vary, and, given the vigorous mixing conditions, the liquid can spill if the tube is not closed tightly. Unfortunately, some of the tubes can be faulty and the liquid spillage is inevitable in at least 1 out of 60 tubes.
  • Page 8 Multiply the dilution factor values to get the OD of the samples. Continue to software manual page 29. 5.3.2. RTS-1 / RTS-1C can be calibrated to detect scattered light of any possible cell with any possible shape and size, but due to difference of light scattering in various cell suspensions, we cannot guarantee the stated measurement range in all conditions.

  • Page 9: Operation

    Operation Recommendations during operation  Remove the falcon tube from the tube socket before connecting or disconnecting the external power supply during operation.  Start operation approximately 15 minutes after switching on the device (some time is necessary for stabilization in the working mode). ...

  • Page 10: Recommended Methods For Microorganism Cultivation

    7.4. It is possible for the end-user to contact the manufacturer for advising or suggesting a required microorganism or strain to be tested. Please contact the R&D department of Biosan at these e-mail addresses: science@biosan.lv, igor@biosan.lv, Igor Bankovsky, consulting biotechnologist on application questions.

  • Page 11: Recommendations For Creating Personal Settings For Cultivation Of Microorganisms

    RS frequency is 30 s (see fig. 5. and fig. 6.). 8.2.1. Legend of experiment (fig. 5.): Personal Bioreactor RTS-1 / RTS-1C was used with 850 nm LED, volume of LB media in 50 ml Falcon tube was 15 ml, Reverse Spin...

  • Page 12: Aeration And Types Of Recommended Tubes

    Figure 5. Influence of Frequency of Reverse Spinning on the Growth kinetics (ΔOD /Δt) vs λ=850nm Time of fermentation (h). Figure 6. Influence of Frequency of Reverse Spinning on the Growth kinetics (ΔOD /Δt) vs λ=850nm Time of fermentation (h). 8.3.

  • Page 13: Factory Calibration Growth Phase Influence On Measurement Accuracy

    8.5. Factory calibration growth phase influence on measurement accuracy During the growth transition of Escherichia coli culture from the exponential growth to the stationary phase, a number of morphological and physiological changes take place, including cell volume decrease and cell shape change. Therefore, if cells taken for referent measurement using spectrophotometer at different stages from stationary phase then the correctness of measurement will be worse than specified.

  • Page 14: Cold Room And Environmental Chamber Influence On Temperature Accuracy Of The Sample

    4°C-10°C and 60°C-70°C must be performed by the user. If any additional questions appear, please contact Biosan RTS support team directly for assistance. E-mail address: igor@biosan.lv . 9.4. Change of optical characteristics of the tube depending on temperature When temperature of the plastic material is changing, i.e.

  • Page 15: Specification

    80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C. Biosan is committed to a continuous program of improvement and reserves the right to alter design and specifications of the equipment without additional notice.

  • Page 16: Maintenance

    11. Maintenance 11.1. If the unit requires maintenance, disconnect the unit from the mains and contact Bio- san or your local Biosan representative. 11.2. All maintenance and repair operations must be performed only by qualified and spe- cially trained personnel.

  • Page 17: Device Calibration For Maintenance

    12.5. Extended warranty. For RTS-1 & RTS-1C, the Smart class models, extended war- ranty is a paid service. Contact your local Biosan representative or our service de- partment through the Technical support section on our website at the link below.

  • Page 18: Eu Declaration Of Conformity

    14. EU Declaration of Conformity Unit type Personal bioreactors Models RTS-1, RTS-1C Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is model code, YY and MM – year and month of production, ZZZZ – unit number. Manufacturer SIA BIOSAN Latvia, LV-1067, Riga, Ratsupites str. 7/2...
  • Page 20 Biosan SIA Ratsupites 7, build.2, Riga, LV-1067, Latvia Phone: +371 67860693, +371 67426137 Fax: +371 67428101 http://www.biosan.lv Edition 3.-4.05 – July 2018...

This manual is also suitable for:

Rts-1c

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