Thermo Scientific IntelliStack Instruction Manual
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Thermo Scientific IntelliStack Instruction Manual

Thermo Scientific IntelliStack Instruction Manual

Incubator shaker
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Thermo Scientific™
IntelliStack™ Incubator Shaker Series
Instruction Manual
Thermo Scientific™ IntelliStack™ Incubator Shaker Series
Thermo Scientific™ IntelliStack™ CO2 Incubator Shaker Series
50171628 Revision C | 2024-11
Please read this user's manual carefully before using the product

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  • Page 1 Thermo Scientific™ IntelliStack™ Incubator Shaker Series Instruction Manual Thermo Scientific™ IntelliStack™ Incubator Shaker Series Thermo Scientific™ IntelliStack™ CO2 Incubator Shaker Series 50171628 Revision C | 2024-11 Please read this user’s manual carefully before using the product...

  • Page 2: Table Of Contents

    Keep this instructions close to the device so that safety instructions and important information are always accessible. If you have any questions that you feel are not covered in sufficient detail in these operating instructions, please contact thermo Scientific for your own safety. 2 / 73...

  • Page 3: General Information

    General Information 1. General Information 1. 1. About this Manual Please read this user's manual carefully before using the product. This manual enables the safe and efficient handling of the device. All the information and instructions in this operating manual comply with the current standards, legal regulations, the latest technological and scientific developments and the knowledge gained from the manufacturer's many years of experience in this field.

  • Page 4: Signal Symbols

    General Information Signal Symbols Observe the information contained in this instruction manual to keep yourself and your environment safe. WARNING - General Refer to instruction manual warning symbol WARNING - Hazard caused Disconnect mains plug from electrical outlet by crushing of hands WARNING - Hazard caused WARNING - Hazard caused by optical Radiation...
  • Page 5 General Information WEEE Conformity: This product is required to comply with European Union's Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EC. It is marked with the adjacent symbol. 1. 4. Maintenance and Field Service Thermo Fisher Scientific recommends having the shaker and accessories serviced once per year by an authorized service technician.

  • Page 6: Safety And Responsibility

    2. 1. Intended Use, Safety Notes, and Misuse The Thermo Scientific™ IntelliStack™ CO2 Incubator Shaker and Thermo Scientific™ IntelliStack™ Incubator Shaker are non-medical device instruments that allow the end user to control the CO2 gas (only for CO2 Incubator Shakers) and humidity levels, shaking speed, time, and temperature inside of the chamber for growth of microorganisms and cells in general laboratory environments.

  • Page 7: Cultivation Vessels To Be Used

    Safety and Responsibility It is the obligation of the operator to make sure that the proper personal protective equipment is used. Intended use also includes following all the instructions in this manual, especially those relating to: The installation site Permissible parameter set points ƒ...

  • Page 8: Qualified Personnel

    Safety and Responsibility Other vessels with the holders designed for them: ƒ Test tubes Media Bottles ƒ ƒ Centrifuge tubes Shaker Flasks up to 5L ƒ ƒ Microtitre plates Deep well plates ƒ ƒ Cultivating organisms of risk group II When cultivating pathogenic organisms of risk group II, special measures must be taken to eliminate contamination.

  • Page 9: General User

    Safety and Responsibility 2. 3. 2. General User The term “user” applies to all persons who come into contact with the device in any way and perform work on or with it. This primarily applies to the following activities, which can be performed by the manufacturer's own specialists or a variety of other persons: Assembly, installation and Maintenance and cleaning...

  • Page 10: Unauthorized Persons

    Safety and Responsibility The "provider" must train the operator on required tasks and inform them of the potential risks of improper conduct. Technicians in Training Persons in this group who are undergoing training or apprenticeships are only permitted to use the device under supervision and in accordance with the instructions of a trained and qualified technician.

  • Page 11: Particular Hazards

    Safety and Responsibility In case of damage to insulation, disconnect the device from the mains immediately ƒ and arrange for it to be repaired. Disconnect the device from the mains before commencing any work on the electrical ƒ system. Always use qualified electricians for any work on the electrical system. ƒ...

  • Page 12: Safety Features

    Safety and Responsibility up in a well-ventilated area. The gas connections on the device must be checked before any cultivation ƒ processes using dangerous gases are initiated. 2. 7. 3. Flammable or Explosive Substances The use or production of flammable or explosive substances is not covered under “intended use”...

  • Page 13: Symbols On The Device

    Safety and Responsibility 2. 9. Symbols on the Device The following symbols (stickers) are attached to the device: WARNING - General Refer to instruction manual warning symbol WARNING - Hazard caused Disconnect mains plug from electrical outlet by crushing of hands WARNING - Hazard caused WARNING - Hazard caused by optical Radiation...
  • Page 14 Safety and Responsibility 2. 11. Safety Technical Specifications Overvoltage Category ll-Connected to Standard Wall Outlet Pollution Degree in Accordance with EN 60664-1.2007 /VDE 0110-1 Protection Class (lEC 60529, Degrees of Ip21 Protection Provided) Equipment Mobility Floor mounted Connection to Mains supply Detactable cord set Operating Conditions Continuous...

  • Page 15: Setup And Function

    Setup and Function 3. Setup and Function 3. 1. Setup of the Basic Unit Exterior User Interface and Display Door with window (opens downwards) Door handle Access port Connections 15 / 73...
  • Page 16 Setup and Function RS485 monitor interface Power switch Mains connection (MAINS) Lamp switch Slot for fuses UV lamp switch Air vents CO2 connection (CO2 IN, optional) 10. Water inlet for Humidity System Interior Finned heating element and axial fans CO2 sensor (optional) (in back wall, not visible) Humidity sensor Lamp...
  • Page 17 50 mm 220 V, 50/60 Hz Up Swing Thermo Scientific™ IntelliStack™ Accessories Description Thermo Scientific™ IntelliStack™ Stand for use with Single or Double 88900000 IntelliStack Shakers, open shelf 88900001 Thermo Scientific™ IntelliStack™ Carboy Kit Thermo Scientific™ IntelliStack™ window cover for light sensitive...

  • Page 18: Basic Functions

    “11. Operational Instruction” on page 54”. Technical data and set points For a detailed description of the technical data and possible maximum rotation speeds, see Page 31 (Loads and Speed Of the IntelliStack Incubator Shakers) 18 / 73...
  • Page 19 3. 2. 2. Standard Function Temperature Control (Heating) Finned heating element and radial fans (in back wall, not visible) The temperature in the incubation chamber is regulated using a Pt100 temperature sensor with a PID controller. Two cross flow fans ensure constant air circulation and keep the temperature distribution in the incubation chamber as constant and gradient free as possible.
  • Page 20 WARNING - During normal operation, small amounts of CO2 are constantly dissipated into the environment. Leaking CO2 can lead to discomfort, loss of consciousness or even death by suffocation, especially in small rooms. The operator is obliged to install a monitoring system which ƒ...
  • Page 21 Use of water outside the specified range will decrease the operating life of the unit and may void warranty. 3. 2. 4. 1. 1 IntelliStack Incubator Shaker Carboy Kit Installation Guide Parts List: 1.
  • Page 22 Triple Stacked Carboy Connection : Carboy Setup Instructions : 1. Insert the HEPA filter into the larger black port. Tighten the black knob until the HEPA filter is securely attached. Attach the included EVA plastic tubing to the metal rod sticking up through the smaller black port. Tighten this knob until it is secure.
  • Page 23 2. When using the water carboy for a double or triple stacked unit, you will need to cut two Or three small pieces off of the EVA tubing, respectively. Attach these pieces to the Y connector (for a double) or 4-way connector (for a triple). (Figure 2) Figure 2: EVA tubing connections for a double stack (left) and triple stack (right) 3.

  • Page 24: Connections And Interfaces

    4. Connect the thin plastic tubing from the back of each incubator shaker to the smaller end of each 2-way tube connector, shown in Figure 5. This completes the setup of the 88900001 IntelliStack Water Carboy. Once it has been filled with water, it is ready for use with your incubator shaker.
  • Page 25 3. 3. 2. Connecting to RS 485 Communication Interface 3. 3. 2. 1. Hardware connection Connect each shaker with network cables through RJ11 connector. The wiring diagram is shown as following. 25 / 73...

  • Page 26: Operating And Indicating Elements

    3. 4. Operating and Indicating Elements 3. 4. 1. Power Switch The power switch is located on the right side of the device. In addition to normal switching on and off, the power switch also works as an emergency switch. NOTE: In case of an emergency shut down via the power switch, all parameter set points are stored and the device...

  • Page 27: Unpacking Guide

    4.2) are accounted for before packaging materials are discarded. After unpacking, if damage is found, report it to the carrier and request a damage inspection. If any parts or accessories are missing, contact Thermo Scientific immediately for support. WARNING - Do not transport or move the instrument without helpers.
  • Page 28 Name Unit Casters User Manual T-Connectors Φ14 Two-way Connectors Φ14 Plug Silicone Tube Φ12×2 Water pipe Fuse Liquid Collection Tray Cross Screwdriver 2# Single open- end Wrench 24 Wrench S10 Screw Plain washer Spring washer Ziplock bag Lifting rods for stacking procedure Spirit Level 28 / 73...

  • Page 29: Specifications

    5. Specifications Temp. Range Room Temp. Minus 18 °C~60°C (Lowest Temp. is 4°C) Temp. Accuracy 0.1 °C Temp. Uniformity ±0.3 °C @37 °C Speed Accuracy 1rpm CO2 Sensor IR CO2 Control Range 0~20% CO2 Control Accuracy ±0.2%( @ 5%) CO2 Display Accuracy 0.1% Recovery Time on Temp.
  • Page 30 Water Sparge Resistant Interface Languages Chinese or English Auto-run after Power Recovery Heat Power ≤850W Cool Power 70~180W AC120V±10% 50/60Hz for 120V models Power Supply AC220V±10% 50/60Hz for 220V models Power ≤1000W Net Weight 310 kg or 683.4 lbs. Internal Dimensions 1005×690×347mm or 39.6"...

  • Page 31: Preparation

    6. Preparation 6. 1. Placement Conditions The instrument must be placed on a firm and level surface. The total weight of the instrument when fully loaded must be taken into consideration. There must be sufficient space left around the instrument. It should be at least 30 cm between the instrument and the wall or any two adjacent instruments.
  • Page 32 6. 3. Space Conditions Configuration Dimensions: 3 stacked IntelliStack Shakers ƒ 32 / 73...

  • Page 33: Configuration Dimensions

    Configuration Dimensions: 2 stacked IntelliStack Shakers with Shaker Stand ƒ Configuration Dimensions: 3 stacked IntelliStack Shakers with up swing top unit ƒ 33 / 73...
  • Page 34 Shaker Stand (optional) Individual units and devices stacked in pairs can be fitted with a 34 cm base. The base has adjustable feet that can be used to level the device. Shaker Stand with open frame - SKU 88900000 Shaker Stand with closed frame - SKU 88900003 34 / 73...

  • Page 35: Safety Instructions

    7. Safety Instructions WARNING - Please make sure to follow the safety instructions Please use a lift or a forklift or other lifting apparatus to raise or move the instrument. ƒ Make sure the power supply voltage matches the voltage of the instrument before it ƒ...
  • Page 36 Loads and Speed Of IntelliStack Incubator Shakers: 25mm Orbit 50mm Orbit Flask Size (ml) ≤Capacity (ml) Max. Speed Max. Speed (rpm) (rpm) 5000 (Thomson) 1700 1500 3000 1000 1400 2000 1000 1000 1000 NOTE: Please select the speed according to the load condition, in order to achieve the best shaker effect.

  • Page 37: Installation

    8. Installation WARNING - Lifting the instrument by hand should be prohibited. Please use a lift or a forklift or other lifting apparatus to raise or move the instrument 8. 1. Tools Requirement Hex wrench S10. Cross screwdriver #2. Single open-end wrench 24. ƒ...

  • Page 38: Drain Connection

    WARNING - Please make sure each instrument is placed level, stable and still. Do not exceed the maximum load of 20kg and the maximum speed, especially when there are shakers stacked. When the devices are stacked, it is recommended to reduce the maximum rotation speed for the middle unit to 250rpm and 220rpm for the top unit.

  • Page 39: Schematic Diagram

    access port is located on the right side of the shaker. (See figure 1) Two-way Connector T-connector Inlet PU Pipe Filter Figure 1 Set the secondary flow control valve to 0.08~ 0.1MPa (0.8 - 1 bar) (as shown in figure 2).
  • Page 40 B. Actual picture NOTICE: Do not move the pressure adjustment rod on the regulator when changing the ƒ Co2 cylinder. It will affect the secondary pressure and will need to reset the valve. The inline tube might fall off if the pressure is too high and maintenance is required.

  • Page 41: Structure

    9. Structure 41 / 73...

  • Page 42: Introduction To Touch Program

    10. Introduction to Touch Program 10. 1. Main interface Displays the current status of the machine. Click to expand the drawer, as shown in the figure 3. Set value display, which can be manually entered byadding subtracting buttons. By clicking this button, the corresponding function can be individually controlled to run/stop, which can be used in conjunction with 6.

  • Page 43: Manual Mode

    NOTE: During the operation of the instrument, you need to log in. The initial account is “admin” and the password is “123456”. The login interface is as shown in the figure 5. If you are not logged in, click anywhere on the interface to pop up the login interface. 10.

  • Page 44: Program Mode

    10. 3. Program Mode This interface allows for program control mode parameter settings. Set the number of segments at the top of the interface, slide settings in the middle of the interface, and cancel and save operations at the bottom of the interface. 10.
  • Page 45 When the login account does not have "Auth Manage" option, it will be displayed as figure 6, and only password modification and login operations can be performed. When the login account has "Auth Manage" option, it is displayed as figure 7. When logging in to an account with "Auth Manage"...
  • Page 46 Figure 12 Figure 13 Figure 14 User deletion deletes the user and their sub-users. For example, if you select user6 in Figure 13 to delete it, the deletion result is Figure 14. user6, user8, and user9 are deleted 10. 5. Settings Click the first button 1 to enter the Instrument settings interface, as detailed in 10.5.1 Click the second button 2 to enter the Calibrations...

  • Page 47: Instrument Settings

    10. 5. 1. Instrument Settings Click the first button 1 to enter the Sleep/Lock interface, as detailed in a. Click the second button 2 to enter the Set time interface, as detailed in b. Click the third button 3 to enter the LAN ID interface, Refer section "10.
  • Page 48 d. Door Signal/Light e. Language 10. 5. 1. 1. Set of RS 485 Communication on User Interface Press Menu icon to enter into setting interface. Click on the Settings button. 48 / 73...
  • Page 49 Click on the Instrument Settings Click on the LAN ID LAN ID and SN code can be set on the interface. LAN ID should be unique for each device, with a maximum of 32 devices can be connected and added here. No restriction for SN code.
  • Page 50 Communication Testing When connecting the Modbus to the user’s software, some basic parameters need to be set: Serial basic settings 9600 Baud 8 Data bits None Parity 1 Stop Bit After completing the basic parameter settings, remote monitoring of the device can be completed by combining the Modbus protocol requirements with the provided Modbus address table.
  • Page 51 a. Temp Calibration b. CO2 Calibration c. Humidity Calibration 10. 5. 3. Alarms Set speed alarm, temperature alarm, communication alarm, CO2 alarm, no gas alarm and humidity alarm. 51 / 73...

  • Page 52: Pid Parameters

    Operation Log screen will show data displayed on ƒ image c below, including user and operator activities on the IntelliStack Shakers Export Data screen is displayed on image d below. ƒ Be sure to enter the desired start and stop dates for the records to be exported.
  • Page 53 NOTE: Over 2 years of data is retained in the IntelliStack internal memory, however it is recommended to download data frequently. When downloading data, use a 8 GB or smaller USB Flash Drive that has FAT32 formatting. a. Alarm History b.

  • Page 54: Operational Instruction

    11. Operational Instruction 11. 1. Temperature Control 11. 1. 1. Temperature Setting In the temperature setting box of the main interface, the actual temperature value is displayed on the left, and the set temperature can be adjusted on the right. You can modify the setting value by clicking the number in the setting box to pop up the keyboard, or click the "+"...

  • Page 55: Speed Control

    11. 2. Speed Control 11. 2. 1. Speed Setting In the speed setting box of the main interface, the actual value is displayed on the left, and the setting speed can be adjusted on the right. You can modify the setting value by clicking the number in the setting box to pop up the keyboard, or click the "+"...
  • Page 56 11. 3. 2. CO2 Calibration Click on the right menu bar, pop up the menu, select the machine setting option, and enter the interface. Click on the calibration interface to enter the concentration calibration. The calibrated concentration points are 1%, 3%, 5%, and 10%.

  • Page 57: Humidity Control

    11. 4. Humidity Control 11. 4. 1. Humidity Setting In the humidity setting box of the main interface, the actual humidity value is displayed on the left, and the humidity can be adjusted on the right. You can modify the setting value by clicking the number in the setting box to pop up the keyboard, or click the "+"...

  • Page 58: Maintenance

    12. Maintenance For the sake of personal, environmental, and material protection, you must inspect, clean and if necessary disinfect the shaker and its accessories on a regular basis. Periodically remove back side plate to clean out dust on the condenser and fan to ƒ...
  • Page 59 WARNING The refrigerant used in the instrument is flammable. ƒ Ensure proper ventilation of the room. ƒ Do not use any mechanical means or other ways to accelerate the ƒ defrosting process. Do not use electrical appliances in the instrument except products ƒ...

  • Page 60: Troubleshooting

    13. Troubleshooting Troubleshooting Error Cause Solution LCD Display Speed difference more Contact local sales/service than 5 rpm Over speed Display Electrical malfunction Contact local sales/service alarm “alarm” Electric motor failure Contact local sales/service Temperature difference Contact local sales/service more than 3 degrees Temperature sensor Over Change sensor...
  • Page 61 Instructions Plug not connected No power Power outlet out of power Power switch off Change to a new fuse with the same specification Display window off Platform not shaking Burned fuse Position: at the Position: inside the power outlet electrical cabinet Press the confirm button to recover High frequency display...

  • Page 62: Optional Accessories

    14. Optional Accessories Part No. Type Size Max Qty Figure 88881135 25ml 88881105 50ml 88881106 100ml 88881107 150ml 88881108 200ml 88881109 Flask Clamp 250ml 88881110 500ml 88881111 1000ml 88881112 2000ml 88881113 3000ml 88881114 5000ml 88881115 500ml Infusion Media Bottle Clamp 88881116 1000ml 88881126...
  • Page 63 Part No. Type Size Max Qty Figure 88881120 40 x14mm 88881137 40 x16mm 88881138 40 x18mm Adjustable Test Tube Rack 88881139 27 x22mm 88881140 21 x30mm 88881141 24 x30mm Micro plate 88881124 96 well holder 88881142 900×540×80mm 88881143 Spring Platform 720×440×80mm 88881144 350×240×80mm...

  • Page 64: Warranty

    15. Warranty THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year warranty period.

  • Page 65: Appendix A - Stacking Procedure For Shaker And Stand

    Appendix A - Stacking Procedure for Shaker and Stand 1. Check the package for the base stand. See pic. 1 Pic. 1 2. Turn the packaging upside down, so that the top cover of the packaging is facing downwards. Remove the stretch film and the package cover, and make the bottom of stand upwards.
  • Page 66 4. Fix the metal feet and casters on the stand. See pic 4. 5. Fine tune the height of the metal feet, about 9cm, slightly higher than casters. See Pic 5. Pic. 5 Pic. 4 6. If need to move the shaker, the height of the metal foot should be slightly lower than that of the casters to move on the ground.
  • Page 67 8 By rotating and fine-tuning the height of the metal feet,confirm that the stand remains stable and the four metal feet are completely sitting on and attaching to the ground. Then adjust the nuts above the metal feet to the highest position and lock the min place. See pic 7. Pic.
  • Page 68 10.Use heavy-duty lifting straps (at least 1T), attach them to the lifting rod,and then use a forklift to lift the machine off the ground. See pic. 12 Pic.12 11. Rotate the four bottom feet of the machine counterclockwise to remove them. See pic 13 Pic.13 68 / 73...
  • Page 69 12. Move the shaker on top of the stand, adjust the position, lower the shaker so that the bottom of the shaker falls on the stand. Make sure the threaded hole at the original red plastic plug position (Figure 6) of the stand is completely aligned with the shaker holes on both sides. See pic 14 Align the Hole Pic.14...
  • Page 70 14. Check and verify all the 4 positions are fully screwed. See Pic 16 and 17 Pic.16 pic.17 15. If need to stack another shaker, Remove the 4 screws and the pads and confirm that the internal threads are intact. See pic 18 and 19 Note: Ensure to remove the plastic sheet from top of the shaker, when shakers are stacked.
  • Page 71 17. Re-install all panels and silicone rod caps, see pic 20 and 21. Note: The casters are intended for use during positioning of the product and should only be used for moving short distances. Once the unit is installed in its final location, the casters must be removed.
  • Page 72 1.The accessories and tools are packed in a blue box, which is usually placed in the gap between the shaker and pallet. 2. Stacking procedure will require a cross screwdriver for disassembling and assembling the side panel; Internal hexagonal wrench and internal hexagonal bolts are used for stacking ; Open wrench, used to fine tune the height of metal feet.
  • Page 73 Country of Origin: China ©2024 Thermo Fisher Scientific Inc. All rights reserved. IntelliStack™ is a registered trademark of Thermo Scientific. Thermo Scientific is a trademark of Thermo Fisher Scientific Inc. All other trademarks mentioned in this manual are the exclusive property of their respective owners. Not all products are available in all countries.

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