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Dionex UltiMate 3000 Series Operating Instructions Manual

Thermostatted column compartments
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UltiMate 3000 Series
Thermostatted Column Compartments
TCC-3x00
Operating Instructions
Revision: 1.2
Date: February 2008
© 2008 Dionex

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  • Page 1 UltiMate 3000 Series Thermostatted Column Compartments TCC-3x00 Operating Instructions Revision: 1.2 Date: February 2008 © 2008 Dionex...
  • Page 2 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Operating Instructions...

  • Page 3: Declaration Of Conformity

    Declaration of Conformity Product: Thermostatted Column Compartment Types: TCC-3000, TCC-3100, and TCC-3200(B) Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Equipment Directive 73/23/EEC changed by 93/68/EEC • EMC Directive 2004/108/EG...
  • Page 4 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Table of Contents Introduction........................1 How to Use This Manual ..................1 Safety Information ....................2 1.2.1 Symbols on the Instrument and in the Manual ..........2 1.2.2 General Safety Precautions................3 1.2.3 Consignes Générales de Sécurité...............
  • Page 6 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Setting Up the TCC in Chromeleon..............29 3.5.1 Loading the USB Driver for the TCC..............29 3.5.2 Installing the TCC....................31 3.5.3 Configuring the TCC ..................32 3.5.3.1 Initial Installation..................32 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings ..35 Preparation for Operation (Startup) ................39...
  • Page 7 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Special Chromeleon Functions ................71 5.6.1 Predictive Performance..................71 5.6.2 TCC Diagnostics....................72 5.6.3 Recording the Temperature Signal ..............73 5.6.4 Operational Qualification und Performance Qualification......73 Shutting Down the TCC ..................74 Routine and Preventive Maintenance ..............
  • Page 8 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page iv Operating Instructions...

  • Page 9: Introduction

    This document is believed to be complete and accurate at the time of publication. In no event shall Dionex be liable for incidental or consequential damages in connection with or arising from the use of this document.

  • Page 10: Safety Information

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 1.2 Safety Information The CE Mark label and cTUVus Mark safety label on the instrument indicate that the instrument is compliant with the related standards (→ pages I and I). 1.2.1 Symbols on the Instrument and in the Manual...

  • Page 11: General Safety Precautions

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Warning: Indicates that failure to take note of the accompanying information may result in personal injury. Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner des blessures corporelles. 1.2.2 General Safety Precautions When working with analytical instrumentation, you should know the potential hazards of using chemical solvents.
  • Page 12 When the panels are removed, dangerous electrical connections will be exposed. Disconnect the TCC from all power sources before removing the panels. • Always replace blown fuses with the original spare fuses from Dionex (→ page 94). • Replace faulty power cords and communication cables.

  • Page 13: Consignes Générales De Sécurité

    Before interrupting operation for several days or more or when preparing the TCC for transport, observe the precautions for shutting down the TCC (→ page 74). • Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex accessories may impair the performance of the instrument. •...
  • Page 14 • Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex (→ page 94). • Remplacez les cordons d'alimentation électrique et les câbles de communication défectueux.
  • Page 15 Shutting Down the TCC (→ page 74). • Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument. •...

  • Page 16: Intended Use

    1 through 13. If there is a question regarding appropriate usage, contact Dionex before proceeding. Dionex is not liable for any damage, material or otherwise, resulting from inappropriate or improper use of the instrument. 1.4 Federal Communications Commission (FCC) Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S.

  • Page 17: Overview

    2 Overview 2.1 Unit Description The TCC is a modern high-quality instrument designed for HPLC analysis, especially as part of the Dionex UltiMate 3000 system. The design has been optimized for minimum dead volume and maximum efficiency. • Thermoelectric elements heat or actively cool the column chamber and all internal components to the desired temperature.

  • Page 18: Operating Principle

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.2 Operating Principle The fundamental requirement for a column compartment for liquid chromatography applications is the ability to maintain the preset temperature as precisely as possible. However, for temperature equalization, temperature stability is more important than the absolute precision of the setting.

  • Page 19: Tcc Configurations

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.3 TCC Configurations 2.3.1 Overview The TCC is available in the following configurations: Description Part No. TCC-3000 Thermostatted column compartment 5722.0000 Basic version with no column switching valve TCC-3100 1x 2P-6P Thermostatted column compartment with one 5722.0010...

  • Page 20: Biocompatible Tcc Version

    To avoid damage to the switching valve, use only the PEEK fitting nuts and ferrules from the TCC accesssories kit or use original Dionex spare parts. Do not use metal fittings and ferrules for the connection.

  • Page 21: Column Switching Valve

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.3.3 Column Switching Valve The TCC can hold one or two column switching valves. The following valve models are available: • 2-position, 6-port column switching valve • 2-position, 10-port column switching valve •...
  • Page 22 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments • Fig. 4 shows a flow schematics for two 6-position, 7-port valves when they are connected for an application with several columns, for example, for method development. Fig. 4: Example for 6 columns on a 6-position, 7-port column switching valve...

  • Page 23: Tcc Interior Components

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.4 TCC Interior Components Fig. 5 illustrates the interior of the TCC. The components installed in your TCC may vary, depending on your TCC configuration and your application. Fig. 5: TCC interior view (example configuration)

  • Page 24: Front Panel Elements

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.5 Front Panel Elements Fig. 6: Front panel view (example) Front Panel Element Description Display Shows information about the TCC, for example: - General information upon power-up (→ page 51) - Status screen (→ page 52) - Various function keys and menus (→...

  • Page 25: Rear Panel

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.6 Rear Panel Fig. 7: Rear panel view Description Power switch (→ page 18) Fuse holder (→ page 18) Main power receptacle (→ page 26) Type label Digital I/O for communication with external devices (→ page 18) USB (Universal Serial Bus) port for connection to the Chromeleon computer (→...

  • Page 26: Power Switch

    TCC. Data is transferred digitally via the appropriate USB cable (→ page 26). To ensure trouble-free operation, all USB cables should be ordered from Dionex. For information about how to connect the TCC to the Chromeleon computer, see sections 3.4.1 and 3.4.2 (→...

  • Page 27: Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.7 Gas and Humidity Sensors The TCC contains two sensors to detect any gas or humidity that may accumulate inside the TCC. When a certain concentration of gas or humidity is reached while the door of the column chamber is closed, the Status LED on the front panel door changes to red.

  • Page 28: Eluent Preconditioner

    An eluent preconditioner brings the solvent to the column temperature before the solvent enters the column. This avoids temperature gradients on the column. Fig. 8: Eluent preconditioner The following eluent preconditioners are available from Dionex as an option for the TCC: Description Part No.* 2 µL eluent preconditioner (stainless steel)

  • Page 29: Chromeleon Software

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2.10 Chromeleon Software The TCC can be controlled by the Chromeleon Chromatography Management System. To control the TCC, an appropriate Chromeleon version and a Timebase Class 1 Chromeleon license are required. Two modes of software control are available: •...
  • Page 30 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page 22 Operating Instructions...

  • Page 31: Installation

    Tips: Immediately report any shipping damage to both the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately. Keep the original shipping container and the packing material. They provide excellent protection for the TCC in case of future transit.

  • Page 32: Positioning The Tcc In The Ultimate 3000 System

    If the TCC is part of an UltiMate 3000 system, for example, for analytical HPLC applications, Dionex recommends stacking the individual modules, for example, as shown in Fig. 10 and interconnecting them on the rear panel as shown in Fig. 11. However, the arrangement of the system modules depends on the application.
  • Page 33 Fig. 11: Example for the rear panel connections on an UltiMate 3000 system (here with VWD-3x00) Tip: Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the computer. However, Dionex recommends interconnecting all modules, and then connecting the system to the Chromeleon computer via only one connection.

  • Page 34: Connecting The Tcc

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3.4 Connecting the TCC 3.4.1 General Information If you want to operate the TCC with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the TCC to the USB port on the Chromeleon computer and turn on the TCC power.

  • Page 35: Connecting The Digital I/O

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3.4.4 Connecting the Digital I/O To connect an external device to the digital I/O ports on the rear panel (→ Fig. 7, page 17), use the (part no. 6000.1004) 6-pin mini-DIN cable from the TCC accessories kit.
  • Page 36 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments The table lists the functions assigned to the connector pins. It also provides information about the core colors and core labels for each pin: Signal Name Signal Level Core Color Core Label Not used...

  • Page 37: Setting Up The Tcc In Chromeleon

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3.5 Setting Up the TCC in Chromeleon This section provides brief instructions for setting up Chromeleon. For details about any of these steps, see the Chromeleon Help. Tip: When the TCC is connected to the Chromeleon computer, verify that the Chromeleon software is installed before turning on the TCC power for the first time.
  • Page 38 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments c) Install Chromeleon. d) Reconnect the USB cable to the computer and turn on the power to the TCC. Vista will now detect the TCC and install the USB software for the TCC automatically.

  • Page 39: Installing The Tcc

    5. Open the Add device to timebase dialog box. To do so, click Add Device on the Edit menu or right-click the timebase and click Add Device on the menu. 6. On the Manufacturers list, click Dionex HPLC: UltiMate 3000 and on the Devices list, click TCC-3x00 Column Compartment.

  • Page 40: Configuring The Tcc

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3.5.3 Configuring the TCC 3.5.3.1 Initial Installation During the installation, Chromeleon connects to the TCC and transfers the settings from the instrument firmware to Chromeleon, setting the options on the wizard pages accordingly.
  • Page 41 TCC and Chromeleon is not interrupted and do not turn off the instrument. Otherwise, the entire firmware may be lost. If the download is not successful, contact Dionex Service. Important: Au cours du téléchargement, assurez-vous que la communication entre l'instrument et Chromeleon n'est pas interrompue et n'arrêtez pas l'instrument.
  • Page 42 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Components Page This page shows which valve models are installed. In addition, you can select for which columns column identification is used. Fig. 16: Components page • Installed components The section shows which column switching valves are installed. Check and change the setting if necessary.

  • Page 43: Changing The Basic Configuration Or Selecting Advanced Settings

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings You can later change the basic configuration settings or select advanced settings. To do so, follow the steps below 1. Start the Server Configuration program (→ page 31).

  • Page 44: Configuration Page

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Configuration Page Use this page to determine whether Chromeleon shall record the temperature as a separate channel and select the pressure unit. Fig. 17: Configuration page • Temperature Signal The check box is selected by default. Accept this setting if you want to record the temperature as a separate channel.
  • Page 45 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Relays Page The Relays page lists all available relays. Select a check box to enable the corresponding relay. If a check box is cleared, the relay will not be available in Chromeleon. To change the relay name or timebase assignment, double-click the relay name (or press the F2 button) and make the changes in the Devices Configuration dialog box.
  • Page 46 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page 38 Operating Instructions...

  • Page 47: Preparation For Operation (Startup)

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4 Preparation for Operation (Startup) 4.1 Overview of Actions Important: The column switching valves are filled with 2-propanol when the TCC is shipped from the factory. During initial operation, make sure that the solvents used are miscible.

  • Page 48: Opening The Front Panel Door

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.2 Opening the Front Panel Door The front panel tilts downward to provide easy access to the components in the column chamber. To open the front panel door, press the release button on top of the door. If the door lock is not released immediately, press the front panel against the enclosure.

  • Page 49: General Precautions For Connecting Capillaries

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.3 General Precautions for Connecting Capillaries When connecting capillaries, observe the following general precautions: • To connect the capillaries to the column switching valve, install only the ferrules and fitting nuts provided in the accessories kit.

  • Page 50: Installing A Separation Column

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.4 Installing a Separation Column Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal parts inside the column chamber while the temperature set point is > 50 °C.
  • Page 51 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 3. If you want to use column identification Attach the column ID chip card to the column. Two chip cards are provided in the TCC accessories kit. Wrap the ribbon around the column, pass the shank of the rivet through a hole, and press down to unite the two parts.

  • Page 52: Connecting The Capillaries To The Column Switching Valve

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.5 Connecting the Capillaries to the Column Switching Valve Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal parts inside the column chamber while the temperature set point is >...

  • Page 53: Connections On A 2-Position, 10-Port Column Switching Valve

    10 (inner diameter of the capillary: 0.13 mm for micro setups, 0.25 mm for analytical setups). 3. Ports 8 and 9 are not used for this setup. Dionex recommends connecting these ports with an additional capillary or closing them with a plug.

  • Page 54: Connections On A 6-Position, 7-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.5.3 Connections on a 6-position, 7-port Column Switching Valve Fig. 25 shows an example for the connections on a 6-position, 7-port column switching valve. Port Connect … Column 1 Column 2 Column 3...

  • Page 55: Connecting The Drain Tubing

    Fig. 27 a. Dionex recommends connecting the drain tubing from the TCC as shown in Fig. 27 b. This is to avoid that the gas sensor in the TCC is influenced by the drain tubing from the other modules.
  • Page 56 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments ♦ The drain tubes must remain below the drain port. If the drain tube is above the drain port, the liquid may flow back into the interior and cause damage to the instrument.

  • Page 57: Equilibrating The System

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 4.7 Equilibrating the System Before using the TCC for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition.
  • Page 58 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments The equilibration panel shows the equilibration status of each instrument in the system. Fig. 30: Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments.

  • Page 59: Operation And Maintenance

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5 Operation and Maintenance The TCC can be controlled by the Chromeleon Chromatography Management System. For details, see section 5.3 (→ page 53). In addition, function keys and menus are available on the front panel display to facilitate operation during, for example, initial installation of the TCC, diagnostics, and maintenance.

  • Page 60: Status Screen

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.2 Status Screen If the self-test was successful, the initial screen changes to the status screen. The instrument type determines the appearance of the screen. Temperature Switching Valves 23.2 °C 10-1 Setpoint: 25.0 °C Fig.

  • Page 61: Operation With Chromeleon

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.3 Operation with Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The TCC is connected to the Chromeleon computer via a USB connection.

  • Page 62: Direct Control

    To open the Commands dialog box for the TCC 1. Open a control panel (any panel is possible). To open a control panel, open the Chromeleon Browser and double-click a control panel in the Dionex Templates/Panels folder. 2. Connect the control panel to the timebase in which the TCC is installed. On the Control menu, select Connect to Timebase, and then select the timebase on the Timebase tab.
  • Page 63 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5. Change the display filter level if necessary. Right-click in the commands list and select the filter level on the menu. Fig. 32: Commands dialog box 6. Verify that the TCC is connected to Chromeleon. If it is not, select Connect to connect the TCC.
  • Page 64 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Fig. 33: TCC Control Panel on the Panel Tabset The control panel provides access to the operating parameters and commands required for routine operation of the TCC. Additional functions are available in the Commands dialog box.

  • Page 65: Automated Control

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.3.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the TCC. Programs can be created automatically with the help of a software wizard or manually by editing an existing program.
  • Page 66 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 34). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.

  • Page 67: Display Screens (Function Keys And Menus)

    Four white spots on the front panel mark the positions of four function keys: Menu, Temp, Valve, and Leak. To show the keys, select the position of the utmost left spot on the front panel display with the Dionex menu pen (part no. 6300.0100). The menu pen is included in the TCC shipment.

  • Page 68: Tcc Menus

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.4.2 TCC Menus Fig. 37 shows an overview of the TCC menus. For information about the general menu layout and structure, see section 5.4.2.1. For information about the commands and parameters that are supported by the menus, see sections 5.4.2.2 through 5.4.2.5 (→...
  • Page 69 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Select an item with the arrow up or down key—the selected item is underlined. Confirm your selection with Select. Back returns you to the previous menu level. The selected menu item or parameter determines which keys appear on the navigation bar: To …...

  • Page 70: Main Menu

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.4.2.2 Main Menu The Main menu provides top-level access to the menu structure. To open the Main menu, show the function keys and select Menu (→ page 59). > Main (1..3) 1. Control 2.

  • Page 71: Control Menu

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.4.2.3 Control Menu On the Control menu, you can make the settings for temperature control and valve control. In addition, you can activate leak detection and specify the sensitivity of the gas and humidity sensors.

  • Page 72: Information Menu

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.4.2.4 Information Menu The Information menu provides information for diagnostic purposes (read-only). > Information (1..3) 1. System 2. Valve 3. Temperature ∨ ∧ Select Back Fig. 41: Information menu To … Select …...

  • Page 73: Configuration Menu

    Key autorepeat—sets wether the arrow up and arrow down keys can be used to page through the items on a menu list if the list contains 5 or more entries: On—yes or Off—no. The menu is reserved for Dionex Service for configuration of the related Valve column switching valve.

  • Page 74: Operational Settings

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.5 Operational Settings 5.5.1 Selecting the Column For a TCC with one or two column switching valves, column programming is performed in Chromeleon. The number of column switching valves, the valve model, and the application determine which commands and properties are used.

  • Page 75: Turning On Column Thermostatting

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.5.2 Turning On Column Thermostatting You can turn column thermostatting on and off and set the desired temperature in Chromeleon or on the front panel display. To turn on column thermostatting from Chromeleon 1.

  • Page 76: Activating Column Identification (Column Id)

    1. Verify that the column ID chip card is attached to the column (→ page 43). 2. Insert the chip card, with the Dionex logo facing up, into one of the four chip card readers. Four chip cards can be used at the same time. When the chip card is installed correctly, the LED next to the slot is green.

  • Page 77: Adjusting The Sensitivity Of The Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments • Operational column properties Chromeleon determines and updates the operational information upon each injection. The operational column properties are, for example, the date of the first injection, the date of the last injection, the maximum flow rate while the column is in use, or the total volume of sample that has been injected onto the column.

  • Page 78: Adjusting The Screen Brightness Or Contrast

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.5.5 Adjusting the Screen Brightness or Contrast You can adjust the screen brightness or screen contrast on the front panel display or from Chromeleon. To adjust the settings from Chromeleon 1. In Chromeleon, open the Commands dialog box for the TCC.

  • Page 79: Special Chromeleon Functions

    Open the Commands dialog box and enter the limits for the predictive performance parameters. For a complete list of available commands and parameters, see the Chromeleon Help. To keep the predictive performance information up-to-date, Dionex recommends the following actions: After you have …...

  • Page 80: Tcc Diagnostics

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Color Description Orange (Only for monitoring Qualification properties) The value has reached the specified limit. However, a Grace Period has been specified during which the component may still be operated. The value has reached the specified limit or the specified grace period has expired.

  • Page 81: Recording The Temperature Signal

    (→ page 36) when the TCC is installed and configured in Chromeleon. With this setting, Chromeleon generates the appropriate channel for recording the temperature signal. Dionex recommends recording the temperature signal in the Chromeleon program. If a problem occurs, the temperature signal channel can provide helpful information to identify and eliminate the source for the problem.

  • Page 82: Shutting Down The Tcc

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 5.7 Shutting Down the TCC Observe the following precautions before interrupting the operation or before shipping the TCC: • For longer periods of inactivity and when using saliferous buffers, rinse the fluid connections with de-ionized water. This avoids salt crystallization, which may impair the instrument performance.
  • Page 83 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments To create the program with the SmartShutdown wizard 1. To open the wizard, click SmartShutdown on the Batch menu. 3. Follow the instructions as they appear on each page of the wizard. For additional information about a page, click Help.

  • Page 84: Routine And Preventive Maintenance

    After using saliferous buffers, rinse the fluid connections with de- ionized water. Annually Have a Dionex service representative check the TCC once a year to prevent, for example, contamination and excessive wear. Tip: Chromeleon supports functions for estimating the lifetime of consumables and diagnostic tests to check the performance of certain TCC components (→...

  • Page 85: Troubleshooting

    Chromeleon Diagnostics Messages section (→ page 81) for a short description of possible causes along with recommended courses of action. If you are unable to eliminate a problem following the instructions given here, contact Dionex Service. Operating Instructions...

  • Page 86: Messages On The Front Panel Display

    The table lists TCC-related messages and suggests appropriate remedial actions. In addition to the messages listed in the table, other messages may appear. In this case, please note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem.
  • Page 87 Turn the TCC off and on again by pressing the power switch on the rear of the TCC and retry. Left MSV error! Contact Dionex Service if the messages appears more often. Left MSV error! The left switching valve cannot be switched to the desired position.
  • Page 88 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments If communication between Chromeleon and the TCC cannot be established, related messages may appear in the Chromeleon Audit Trail. Message Remedial Action TCC-3x00@USB-01610103 - The USB connection between the TCC and the Chromeleon server Device not found on the USB.

  • Page 89: Chromeleon Diagnostics Messages

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 6.3 Chromeleon Diagnostics Messages If the TCC fails a diagnostics test, perform the instructions given here. If the detector still fails the test, contact Dionex Service. Gas Sensor Test failed Test Result Possible Cause...

  • Page 90: Operating Problems

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 6.4 Operating Problems The table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information, also see the manuals for the other modules of an UltiMate 3000 system.
  • Page 91 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Problem Probable Cause Remedial Action The temperature does not The ambient temperature is too Reduce the ambient change for some time high or the set point is lower than temperature (for example, by...
  • Page 92 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page 84 Operating Instructions...

  • Page 93: Service

    • Before you return any instrument to Dionex for repair, contact Dionex Service or your local distributor. An RMA number (Return Material Authorization number) is required in order to track your instrument. Always use the original packaging and observe the packing instructions (→...

  • Page 94: Column Switching Valve

    • To connect the capillaries to the column switching valve, install only the ferrules and fitting nuts from the TCC accessories kit or original Dionex spare parts. Ferrule for stainless steel valve for biocompatible valve...

  • Page 95: Inspecting The Column Switching Valve For Leakage

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.2.1 Inspecting the Column Switching Valve for Leakage Several drain holes are provided on the stator ring. If liquid droplets appear on one or more drain holes, this may indicate valve leakage. Stator ring Drain hole Fig.

  • Page 96: Replacing The Valve Pod

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.2.2 Replacing the Valve Pod Description Part No. Stainless steel column switching valves Pod for 2-position, 6-port valve 6722.9013 2-position, 10-port valve 6722.9023 6-position, 7-port valve 6722.9035 The rotor seal in the valve pod is the appropriate choice for pH values <...
  • Page 97 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments As an alternative You can use the so-called "Pod Popper" to facilitate the removal of the valve pod. Place the tab of the popper into one of the drain holes on the stator ring (→ Fig. 48). Unscrew the spanner (using only your hand) while holding the popper in place with pressure.
  • Page 98 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Valve coupling Shaft Valve body Fig. 50: Separation distance between valve coupling and body 6. Tighten the spanner firmly with your hand only (use no tool). If the spanner is too tight, the valve cannot be switched. If you attempt to switch the valve, a message will appear, for example, Left (or Right) MSV error! Retrying…...

  • Page 99: Replacing The Valve Stator

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.2.3 Replacing the Valve Stator Description Part No. Stainless steel column switching valves Stator for 6722.9011 2 position, 6 port valve 6722.9021 2 position, 10 port valve 6722.9031 6 position, 7 port valve...

  • Page 100: Rotor Seal And Stator Face Seal

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.2.4 Rotor Seal and Stator Face Seal The valve type (standard or biocompatible) and the pH value of the application determine the type of seals: Description Part No. Stainless steel column switching valve and pH value of the application < 10...

  • Page 101: Replacing The Rotor Seal And Stator Face Seal

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.2.4.2 Replacing the Rotor Seal and Stator Face Seal 1. Remove the stator screws located in the stator (→ Fig. 51, page 91), and then remove the stator with stator face seal from the valve body.

  • Page 102: Replacing The Main Power Fuses

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 7.3 Replacing the Main Power Fuses Warning: Before replacing the fuses, turn off the TCC. Be sure to disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez l'instrument. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur.

  • Page 103: Technical Information

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 8 Technical Information Temperature range: 5 °C to 85 °C (max. 18 °C below ambient) ±0.5 °C Temperature accuracy: Temperature stability: ±0.1 °C Temperature precision: ±0.1 °C Up to 6 columns, max. 30 cm length...
  • Page 104 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page 96 Operating Instructions...

  • Page 105: Accessories, Spare Parts, And Consumables

    USB cable (0.5 m, type A to type B, USB 2.0) 6720.8910 6-pin Mini-DIN signal cable (5 m) 6000.1004 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Operating Instructions Page 97...
  • Page 106 USB cable (0.5 m, type A to type B, USB 2.0) 6720.8910 6-pin Mini-DIN signal cable (5 m) 6000.1004 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Page 98 Operating Instructions...
  • Page 107 USB cable (0.5 m, type A to type B, USB 2.0) 6720.8910 6-pin Mini-DIN signal cable (5 m) 6000.1004 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Operating Instructions Page 99...

  • Page 108: Optional Accessories

    6-pin Mini-DIN signal cable (5 m) 6000.1004 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. 9.2 Optional Accessories Description Part No. 2 µL eluent preconditioner (stainless steel) 6722.0530...

  • Page 109: Consumables And Spare Parts

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 9.3 Consumables and Spare Parts Description Part No.* Column brackets (set of 2 brackets) 6720.0601 Column clip kit (set of 6 clips) 6722.0290 Column ID (set of 5 IDs) 6710.1505 Eluent preconditioner (2 µL) (stainless steel) 6722.0530...
  • Page 110 2 m tubing (OD x ID: 8 mm x 4.8 mm ID) 5 Tee pieces 2 elbows Wrench (size 2.5 mm) 6146.2625 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Page 102 Operating Instructions...

  • Page 111: Index

    UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments 10 Index Connection Chromeleon Computer........26 Digital I/O............. 27 Accessories Drain Tubing ..........47 Optional ............100 Power............26 Standard ............97 USB .............. 26 Consumables ........... 101 Control (Automated) ........53, 57 Back ..............61 Control (Direct) ...........
  • Page 112 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Function Key .............59 Main.............. 62 Arrow down ..........61 Overview ............60 Arrow right ...........61 Structure ............60 Arrow up............61 Messages ............78 Back ..............61 Module Address ..........33 Cancel ............61 Clear............61, 78 Execute ............61 Ok..............
  • Page 113 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Screen Brightness ..........70 Actions............39 Screen Contrast..........70 Capillaries............. 41 Select ..............61 Equilibration ..........49 Sensor Sensitivity ..........69 Stator Face Seal ..........92 Separation Column Installation ......42 Status Screen ............. 52 Server Configuration Program......31 System Wellness..........
  • Page 114 UltiMate 3000 Series: TCC-3x00 Thermostatted Column Compartments Page 106 Operating Instructions...

This manual is also suitable for:

Ultimate tcc-3100Ultimate tcc-3200Ultimate tcc-3200b

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