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Dionex UltiMate 3000 Series Operating Instructions Manual

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UltiMate 3000 Series
Column Compartment
TCC-3200
Operating Instructions
Revision:
1.0
Date: August 2008
© 2008 Dionex

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  • Page 1 UltiMate 3000 Series Column Compartment TCC-3200 Operating Instructions Revision: Date: August 2008 © 2008 Dionex...
  • Page 2 UltiMate 3000 Series: TCC-3200 Column Compartment Operating Instructions...

  • Page 3: Declaration Of Conformity

    Declaration of Conformity Product: Column Compartment Types: TCC-3200 Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Equipment Directive 73/23/EEC changed by 93/68/EEC • EMC Directive 2004/108/EG The electrical safety of the products was evaluated based on the following standard: •...
  • Page 4 UltiMate 3000 Series: TCC-3200 Column Compartment Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: TCC-3200 Column Compartment Table of Contents Introduction........................1 How to Use This Manual ..................1 Safety Information ....................2 1.2.1 Symbols on the Instrument and in the Manual ..........2 1.2.2 General Safety Precautions................3 1.2.3 Consignes Générales de Sécurité............... 5 Intended Use ......................
  • Page 6 UltiMate 3000 Series: TCC-3200 Column Compartment 3.5.3 Configuring the TCC ..................31 3.5.3.1 Initial Installation..................31 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings ..34 Preparation for Operation (Startup) ................37 Overview of Actions ....................37 Opening the Front Panel Door ................38 General Precautions for Connecting Capillaries..........39 Installing the Valve Pod..................40...
  • Page 7 UltiMate 3000 Series: TCC-3200 Column Compartment Shutting Down the TCC ..................73 Routine and Preventive Maintenance ..............75 Troubleshooting ......................77 Overview......................77 Messages on the TCC Display................78 Chromeleon Diagnostics Messages ..............81 Operating Problems ..................... 82 Service.......................... 85 General Notes ......................
  • Page 8 UltiMate 3000 Series: TCC-3200 Column Compartment Page iv Operating Instructions...

  • Page 9: Introduction

    This document is believed to be complete and accurate at the time of publication. In no event shall Dionex be liable for incidental or consequential damages in connection with or arising from the use of this document.

  • Page 10: Safety Information

    UltiMate 3000 Series: TCC-3200 Column Compartment 1.2 Safety Information The CE Mark label and cTUVus Mark safety label on the TCC indicate that the instrument is compliant with the related standards (→ pages I and I). 1.2.1 Symbols on the Instrument and in the Manual...

  • Page 11: General Safety Precautions

    UltiMate 3000 Series: TCC-3200 Column Compartment Warning: Indicates that failure to take note of the accompanying information may result in personal injury. Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner des blessures corporelles. 1.2.2 General Safety Precautions When working with analytical instrumentation, you should know the potential hazards of using chemical solvents.
  • Page 12 When the panels are removed, dangerous electrical connections will be exposed. Disconnect the TCC from all power sources before removing the panels. • Always replace blown fuses with the original spare fuses from Dionex (→ page 94). • Replace faulty power cords and communication cables.

  • Page 13: Consignes Générales De Sécurité

    Before interrupting operation for several days or more or when preparing the TCC for transport, observe the precautions for shutting down the TCC (→ page 73). • Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex accessories may impair the performance of the instrument. •...
  • Page 14 • Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex (→ page 94). • Remplacez les cordons d'alimentation électrique et les câbles de communication défectueux.
  • Page 15 Shutting Down the TCC (→ page 73). • Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument. •...

  • Page 16: Intended Use

    - pH range: Depends on the rotor seal in the switching valve (→ section 7.2.4, page 92) If there is a question regarding appropriate usage, contact Dionex before proceeding. Dionex is not liable for any damage, material or otherwise, resulting from inappropriate or improper use of the instrument.

  • Page 17: Overview

    2 Overview 2.1 Unit Description The TCC is a modern high-quality instrument designed for HPLC analysis, especially as part of the Dionex UltiMate 3000 system. The design has been optimized for minimum dead volume and maximum efficiency. • Thermoelectric elements heat or actively cool the column chamber and all internal components to the desired temperature.

  • Page 18: Operating Principle

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.2 Operating Principle The fundamental requirement for a column compartment for liquid chromatography applications is the ability to maintain the preset temperature as precisely as possible. However, for temperature equalization, temperature stability is more important than the absolute precision of the setting.

  • Page 19: Tcc Configurations

    TCC-3200 Column Compartment 5722.0035 Basic version without valve pods. Up to two pods can be installed. The following valve pods are available from Dionex for use with the TCC: Valve Pods* for Part No. TitanHP™column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port valve** 6722.9013...

  • Page 20: Column Switching Valve

    UltiMate 3000 Series: TCC-3200 Column Compartment Together with a dual low-pressure gradient pump (DGP-3600) of the UltiMate 3000 system • A TCC with 2-position, 6-port switching valves is the appropriate choice for applications, such as matrix separation or concentration of components (→ Fig. 1, page 12).
  • Page 21 UltiMate 3000 Series: TCC-3200 Column Compartment • Fig. 2 shows a flow schematics for a 2-position, 10-port column swichting valve when it is connected for an application with overlapping injection with offline re-equilibration of the column. In position 10-1, column 1 analyzes while column 2 re-equilibrates. In position 1-2, column 2 analyzes, while column 1 re-equilibrates.

  • Page 22: Front Panel Elements

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.4 Front Panel Elements Fig. 4: Front panel view (example) Front Panel Element Description Display Shows information about the TCC, for example: - General information upon power-up (→ page 51) - Status screen (→ page 52) - Various function keys and menus (→...

  • Page 23: Rear Panel

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.5 Rear Panel Fig. 5: Rear panel view Description Power switch (→ page 16) Fuse holder (→ page 16) Main power receptacle (→ page 25) Type label Digital I/O for communication with external devices (→ page 16) USB (Universal Serial Bus) port for connection to the Chromeleon computer (→...

  • Page 24: Power Switch

    TCC. Data is transferred digitally via the appropriate USB cable (→ page 25). To ensure trouble-free operation, all USB cables should be ordered from Dionex. For information about how to connect the TCC to the Chromeleon computer, see sections 3.4.1 and 3.4.2 (→...

  • Page 25: Interior Components

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.6 Interior Components Fig. 6 illustrates the interior of the TCC. The components installed in your TCC may vary, depending on your TCC configuration and your application. Fig. 6: TCC interior view (example configuration)

  • Page 26: Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.7 Gas and Humidity Sensors The TCC contains two sensors to detect any gas or humidity that may accumulate inside the TCC. When a certain concentration of gas or humidity is reached inside the TCC while the door of the column chamber is closed, the Status LED on the front panel door changes to red and a message appears on the TCC display and in the Chromeleon Audit trail.

  • Page 27: Pre-Column Heater

    • If you want to increase the flow rate to 2 mL/min or if the temperature difference is more likely to be 30 K, Dionex recommends installing a pre-column heater with a volume of 11 µL. The pre-column heaters can be installed at different positions in the column chamber. A maximum of six pre-column heaters can be installed at the same time.

  • Page 28: Chromeleon Software

    UltiMate 3000 Series: TCC-3200 Column Compartment 2.10 Chromeleon Software The TCC can be controlled by the Chromeleon Chromatography Management System. To control the TCC, an appropriate Chromeleon version and a Timebase Class 1 Chromeleon license are required. Two modes of software control are available: •...

  • Page 29: Installation

    Tips: Immediately report any shipping damage to both the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately. Keep the original shipping container and the packing material. They provide excellent protection for the TCC in case of future transit.
  • Page 30 UltiMate 3000 Series: TCC-3200 Column Compartment 3. Remove the foam spacers, and then remove the polythene packaging. 4. Remove the foam insert from the door latch. The insert secures the front panel door during shipment. 5. Before connecting the TCC to the power source, wait approximately 4 hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate.

  • Page 31: Positioning The Tcc In The Ultimate 3000 System

    If the TCC is part of an UltiMate 3000 system, for example, for analytical HPLC applications, Dionex recommends stacking the individual modules, for example, as shown in Fig. 9 and interconnecting them on the rear panel as shown in Fig. 10. However, the arrangement of the system modules depends on the application.
  • Page 32 Fig. 10: Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer. However, Dionex recommends interconnecting all modules, and then connecting the system to the Chromeleon computer via only one connection.

  • Page 33: Connecting The Tcc

    UltiMate 3000 Series: TCC-3200 Column Compartment 3.4 Connecting the TCC 3.4.1 General Information If you want to operate the TCC with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the TCC to the USB port on the Chromeleon computer and turn on the TCC power.

  • Page 34: Connecting The Digital I/O

    UltiMate 3000 Series: TCC-3200 Column Compartment 3.4.4 Connecting the Digital I/O To connect an external device to the digital I/O ports on the rear panel (→ Fig. 5, page 15), use the (part no. 6000.1004) 6-pin mini-DIN cable from the TCC accessories kit.
  • Page 35 UltiMate 3000 Series: TCC-3200 Column Compartment The table lists the functions assigned to the connector pins and the color and label of the cable wire connected to each pin: Signal Name Signal Level Wire Color Wire Label Not used Pink...

  • Page 36: Setting Up The Tcc In Chromeleon

    UltiMate 3000 Series: TCC-3200 Column Compartment 3.5 Setting Up the TCC in Chromeleon This section provides brief instructions for setting up Chromeleon. For details about any of these steps, see the Chromeleon Help. Tip: When the TCC is connected to the Chromeleon computer, verify that the Chromeleon software is installed before turning on the TCC power for the first time.
  • Page 37 UltiMate 3000 Series: TCC-3200 Column Compartment c) Install Chromeleon. d) Turn on the power to the TCC. Vista will now detect the TCC and install the USB software for the TCC automatically. 8. Windows XP and 2000 will automatically detect the new TCC and launch the Found New Hardware Wizard, which guides you through the USB installation.

  • Page 38: Installing The Tcc

    5. Open the Add device to timebase dialog box. To do so, click Add Device on the Edit menu or right-click the timebase and click Add Device on the menu. 6. On the Manufacturers list, click Dionex HPLC: UltiMate 3000 and on the Devices list, click TCC-3x00(RS) Column Compartment.

  • Page 39: Configuring The Tcc

    UltiMate 3000 Series: TCC-3200 Column Compartment 3.5.3 Configuring the TCC 3.5.3.1 Initial Installation During the installation, Chromeleon connects to the TCC and transfers the settings from the instrument firmware to Chromeleon, setting the options on the wizard pages accordingly. Verify that the default settings are correct and make additional settings if needed. You may reopen the configuration pages later again to change the settings (→...
  • Page 40 UltiMate 3000 Series: TCC-3200 Column Compartment • Demo Mode Verify that check box is cleared. In the Demo Mode, Chromeleon simulates the functions of the TCC. If the Demo Mode is enabled, the Module address input field will be unavailable. If you exit this page without having entered a module address, the Demo Mode will be enabled automatically.
  • Page 41 UltiMate 3000 Series: TCC-3200 Column Compartment Components Page This page shows which valve models are installed. In addition, you can select for which columns column identification is used. Fig. 15: Components page • Left Valve and Right Valve Shows which valve models are installed. Check and change the setting if necessary.

  • Page 42: Changing The Basic Configuration Or Selecting Advanced Settings

    UltiMate 3000 Series: TCC-3200 Column Compartment 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings You can later change the basic configuration settings or select advanced settings. To do so, follow the steps below 1. Start the Server Configuration program (→ page 30).

  • Page 43: Configuration Page

    UltiMate 3000 Series: TCC-3200 Column Compartment Configuration Page Use this page to determine whether Chromeleon shall record the temperature as a separate channel and select the pressure unit. Fig. 16: Configuration page • Temperature Signal The check box is selected by default. Accept this setting if you want to record the temperature as a separate channel.
  • Page 44 UltiMate 3000 Series: TCC-3200 Column Compartment Relays Page The Relays page lists all available relays. Select a check box to enable the corresponding relay. If a check box is cleared, the relay will not be available in Chromeleon. To change the relay name or timebase assignment, double-click the relay name (or press the F2 button) and make the changes in the Devices Configuration dialog box.

  • Page 45: Preparation For Operation (Startup)

    UltiMate 3000 Series: TCC-3200 Column Compartment 4 Preparation for Operation (Startup) 4.1 Overview of Actions Important: The column switching valves are filled with 2-propanol when the TCC is shipped from the factory. During initial operation, make sure that the solvents used are miscible. Otherwise, use an appropriate intermediate solvent.

  • Page 46: Opening The Front Panel Door

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.2 Opening the Front Panel Door The front panel tilts downward to provide easy access to the components in the column chamber. To open the front panel door, press the release button on top of the door. If the door lock is not released immediately, press the front panel against the enclosure.

  • Page 47: General Precautions For Connecting Capillaries

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.3 General Precautions for Connecting Capillaries When connecting capillaries to the switching valves and columns, observe the following general precautions: • To connect the capillaries to the column switching valve, install only the ferrules and fitting nuts provided in the accessories kit.

  • Page 48: Installing The Valve Pod

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.4 Installing the Valve Pod Valve Pods* for Part No. TitanHP™ column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port valve** 6722.9013 6-position, 7-port valve** 6722.9035 Suitable for pressures < 345 bar (5000 psi) 2-position, 10-port valve** 6722.9023...
  • Page 49 UltiMate 3000 Series: TCC-3200 Column Compartment 4. Push the pod completely into the valve drive body. Be sure to install the pod in the correct orientation: Align the large slot in the pod with the matching runner in the valve driver body.

  • Page 50: Troubleshooting Tip

    UltiMate 3000 Series: TCC-3200 Column Compartment Troubleshooting Tip If you cannot push the pod completely into the valve driver body, the position of the valve coupling does not match the position of the valve drive. To remedy the situation: 1. Switch the valve into another position and try again.

  • Page 51: Installing A Separation Column

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.5 Installing a Separation Column Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal parts inside the column chamber while the temperature set point is > 50 °C.
  • Page 52 UltiMate 3000 Series: TCC-3200 Column Compartment 3. If you want to use column identification Attach the column ID chip card to the column. Two chip cards are provided in the TCC accessories kit. Wrap the ribbon around the column, pass the shank of the rivet through a hole, and press down to unite the two parts.

  • Page 53: Connecting The Capillaries To The Column Switching Valve

    When connecting capillaries to the biocompatible valve pod, use only the PEEK fitting nuts and ferrules from the TCC accessories kit or original Dionex spare parts. Do not use metal fittings and ferrules for the connection; they may cause damage to the valve.

  • Page 54: Connections On A 2-Position, 6-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.6.1 Connections on a 2-position, 6-port Column Switching Valve Fig. 25 shows a typical example for the connections on a 2-position, 6-port column switching valve. Port Connect the capillary …. from the autosampler or pump...

  • Page 55: Connections On A 2-Position, 10-Port Column Switching Valve

    10 (inner diameter of the capillary: 0.13 mm for micro setups, 0.25 mm for analytical setups). 3. Ports 8 and 9 are not used for this setup. Dionex recommends connecting these ports with an additional capillary or closing them with a plug.

  • Page 56: Connections On A 6-Position, 7-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.6.3 Connections on a 6-position, 7-port Column Switching Valve Fig. 27 shows an example for the connections on a 6-position, 7-port column switching valve. Port Connect … Column 1 Column 2 Column 3 Column 4...

  • Page 57: Equilibrating The System

    UltiMate 3000 Series: TCC-3200 Column Compartment 4.8 Equilibrating the System Before using the TCC for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition.
  • Page 58 UltiMate 3000 Series: TCC-3200 Column Compartment Fig. 29: Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments. For information about the TCC menus, see section 5.4.2 (→ page 60). For information about the menus of other system modules, see the Operating Instructions for the module.

  • Page 59: Operation And Maintenance

    UltiMate 3000 Series: TCC-3200 Column Compartment 5 Operation and Maintenance The TCC can be controlled by the Chromeleon Chromatography Management System. For details, see section 5.3 (→ page 53). In addition, function keys and menus are available on the TCC display to facilitate operation during, for example, initial installation of the TCC, diagnostics, and maintenance.

  • Page 60: Status Screen

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.2 Status Screen If the self-test was successful, the initial screen changes to the status screen. The instrument type determines the appearance of the screen. Temperature Switching Valves 23.2 °C 10-1 Setpoint: 25.0 °C...

  • Page 61: Operation With Chromeleon

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.3 Operation with Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The TCC is connected to the Chromeleon computer via a USB connection.

  • Page 62: Direct Control

    To open the Commands dialog box for the TCC 1. Open a control panel (any panel is possible). To open a control panel, open the Chromeleon Browser and double-click a control panel in the Dionex Templates/Panels folder. 2. Connect the control panel to the timebase in which the TCC is installed. On the Control menu, select Connect to Timebase, and then select the timebase on the Timebase tab.
  • Page 63 UltiMate 3000 Series: TCC-3200 Column Compartment 5. Change the display filter level if necessary. Right-click in the commands list and select the filter level on the menu. Fig. 31: Commands dialog box 6. Verify that the TCC is connected to Chromeleon. If it is not, select Connect to connect the TCC.
  • Page 64 UltiMate 3000 Series: TCC-3200 Column Compartment Fig. 32: TCC Control Panel on the Panel Tabset The control panel provides access to the operating parameters and commands required for routine operation of the TCC. Additional functions are available in the Commands dialog box.

  • Page 65: Automated Control

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.3.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the TCC. Programs can be created automatically with the help of a software wizard or manually by editing an existing program.
  • Page 66 UltiMate 3000 Series: TCC-3200 Column Compartment 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 33). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.

  • Page 67: Display Screens (Function Keys And Menus)

    Four white spots on the front panel mark the positions of four function keys: Menu, Temp, Valve, and Leak. To show the keys, select the position of the utmost left spot on the TCC display with the Dionex menu pen (part no. 6300.0100). The menu pen is included in the TCC shipment.

  • Page 68: Tcc Menus

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.4.2 TCC Menus Fig. 36 shows an overview of the TCC menus. For information about the general menu layout and structure, see section 5.4.2.1. For information about the commands and parameters that are supported by the menus, see sections 5.4.2.2 through 5.4.2.5 (→...
  • Page 69 UltiMate 3000 Series: TCC-3200 Column Compartment Select an item with the arrow up or down key—the selected item is underlined. Confirm your selection with Select. Back returns you to the previous menu level. The selected menu item or parameter determines which keys appear on the navigation bar: To …...

  • Page 70: Main Menu

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.4.2.2 Main Menu The Main menu provides top-level access to the menu structure. To open the Main menu, show the function keys and select Menu (→ page 59). > Main (1..3) 1. Control 2. Information 3.

  • Page 71: Control Menu

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.4.2.3 Control Menu On the Control menu, you can make the settings for temperature control and valve control. In addition, you can activate leak detection and specify the sensitivity of the gas and humidity sensors.

  • Page 72: Information Menu

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.4.2.4 Information Menu The Information menu provides information for diagnostic purposes (read-only). > Information (1..3) 1. System 2. Valve 3. Temperature ∨ ∧ Select Back Fig. 40: Information menu To … Select … See general information about the TCC, for example, System firmware version, serial number, and operating hours.

  • Page 73: Configuration Menu

    Back Fig. 41: Configuration menu To … Select … Restore the Dionex standard settings. Reset to factory defaults In the Reset to factory defaults? dialog box, select OK to confirm the restore or select Cancel to keep your settings. Set the display and function key parameters: Display &...

  • Page 74: Operational Settings

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.5 Operational Settings 5.5.1 Selecting the Column For a TCC with one or two column switching valves, column programming is performed in Chromeleon. The number of column switching valves, the valve model, and the application determine which commands and properties are used.

  • Page 75: Turning On Column Thermostatting

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.5.2 Turning On Column Thermostatting You can turn column thermostatting on and off and set the desired temperature in Chromeleon or on the TCC display. To turn on column thermostatting from Chromeleon 1. In Chromeleon, open the Commands dialog box.

  • Page 76: Activating Column Identification (Column Id)

    1. Verify that the column ID chip card is attached to the column (→ page 44). 2. Insert the chip card, with the Dionex logo facing up, into one of the four chip card readers. Four chip cards can be used at the same time. When the chip card is installed correctly, the LED next to the reader is green.

  • Page 77: Adjusting The Sensitivity Of The Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3200 Column Compartment • Operational column properties Chromeleon determines and updates the operational information upon each injection. The operational column properties are, for example, the date of the first injection, the date of the last injection, the maximum flow rate while the column is in use, or the total volume of sample that has been injected onto the column.

  • Page 78: Adjusting The Screen Brightness Or Contrast

    UltiMate 3000 Series: TCC-3200 Column Compartment 5.5.5 Adjusting the Screen Brightness or Contrast You can adjust the screen brightness or screen contrast on the TCC display or from Chromeleon. To adjust the settings from Chromeleon 1. In Chromeleon, open the Commands dialog box for the TCC.

  • Page 79: Special Chromeleon Functions

    Open the Commands dialog box for the TCC and enter the limits for the predictive performance parameters. For a list of the commands and parameters that are supported for the TCc, see the Chromeleon Help. To keep the predictive performance information up-to-date, Dionex recommends the following actions: After you have …...

  • Page 80: Tcc Diagnostics

    UltiMate 3000 Series: TCC-3200 Column Compartment Color Description Orange (Only for monitoring Qualification properties) The value has reached the specified limit. However, a Grace Period has been specified during which the component may still be operated. The value has reached the specified limit or the specified grace period has expired.

  • Page 81: Recording The Temperature Signal

    (→ page 35) when the TCC is installed and configured in Chromeleon. With this setting, Chromeleon generates the appropriate channel for recording the temperature signal. Dionex recommends recording the temperature signal in the Chromeleon program. If a problem occurs, the temperature signal channel can provide helpful information to identify and eliminate the source for the problem.
  • Page 82 UltiMate 3000 Series: TCC-3200 Column Compartment Shutdown Program A shutdown program automates shutdown of the HPLC system. The main program steps are: • The pump flow is automatically stopped at the end of the program. • Certain system components and functions are turned off (for example, detector lamps, temperature control).

  • Page 83: Routine And Preventive Maintenance

    After using saliferous buffers, rinse the fluid connections with de- ionized water. Annually Have a Dionex service representative check the TCC once a year to prevent, for example, contamination and excessive wear. Tip: Chromeleon supports functions for estimating the lifetime of consumables and diagnostic tests to check the performance of certain TCC components (→...
  • Page 84 UltiMate 3000 Series: TCC-3200 Column Compartment Page 76 Operating Instructions...

  • Page 85: Troubleshooting

    Chromeleon Diagnostics Messages section (→ page 81) for a short description of possible causes along with recommended courses of action. If you are unable to eliminate a problem following the instructions given here, contact Dionex Service. Operating Instructions...

  • Page 86: Messages On The Tcc Display

    The table lists TCC-related messages and suggests appropriate remedial actions. In addition to the messages listed in the table, other messages may appear. In this case, please note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem.
  • Page 87 Turn the TCC off and on again by pressing the power switch on the rear of the TCC and retry. Left MSV error! Contact Dionex Service if the messages appears more often. Left MSV error! The left switching valve cannot be switched to the desired position.
  • Page 88 UltiMate 3000 Series: TCC-3200 Column Compartment If communication between Chromeleon and the TCC cannot be established, related messages may appear in the Chromeleon Audit Trail. Message Remedial Action TCC-3x00@USB-01610103 - The USB connection between the TCC and the Chromeleon server Device not found on the USB.

  • Page 89: Chromeleon Diagnostics Messages

    UltiMate 3000 Series: TCC-3200 Column Compartment 6.3 Chromeleon Diagnostics Messages If the TCC fails a diagnostics test, perform the instructions given here. If the detector still fails the test, contact Dionex Service. Gas Sensor Test failed Test Result Possible Cause...

  • Page 90: Operating Problems

    UltiMate 3000 Series: TCC-3200 Column Compartment Test Result Possible Cause Remedial Action Timeout waiting for The thermal unit may be Repeat the test. temperature to reach defective. (exceed, approach) room temperature during heating (or cooling) phase. 6.4 Operating Problems The table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions.
  • Page 91 UltiMate 3000 Series: TCC-3200 Column Compartment Problem Probable Cause Remedial Action The system has very high A valve (if installed) is blocked. Check the valve for backpressure (Cont'd) indications of blockage. It might be necessary to replace the valve. The system has very high The column is contaminated or Rinse or replace the column.
  • Page 92 UltiMate 3000 Series: TCC-3200 Column Compartment Page 84 Operating Instructions...

  • Page 93: Service

    • Before you return any instrument to Dionex for repair, contact Dionex Service or your local distributor. An RMA number (Return Material Authorization number) is required in order to track your instrument. Always use the original packaging and observe the packing instructions (→...

  • Page 94: Column Switching Valve

    • To connect the capillaries to the column switching valve, install only the ferrules and fitting nuts from the TCC accessories kit or original Dionex spare parts. Ferrule for stainless steel valve for biocompatible valve...

  • Page 95: Inspecting The Column Switching Valve For Leakage

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.2.1 Inspecting the Column Switching Valve for Leakage Several drain holes are provided on the stator ring. If liquid droplets appear on one or more drain holes, this may indicate valve leakage. Stator ring Drain hole Fig.

  • Page 96: Replacing The Valve Pod

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.2.2 Replacing the Valve Pod Valve Pods* for Part No. TitanHP™ column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port valve** 6722.9013 6-position, 7-port valve** 6722.9035 Suitable for pressures < 345 bar (5000 psi) 2-position, 10-port valve** 6722.9023...
  • Page 97 UltiMate 3000 Series: TCC-3200 Column Compartment As an alternative You can use the "Pod Popper" to facilitate the removal of the valve pod. Place the tab of the popper into one of the drain holes on the stator ring. Unscrew the spanner (using only your hand) while holding the popper in place with pressure.
  • Page 98 UltiMate 3000 Series: TCC-3200 Column Compartment Troubleshooting Tip If you cannot push the pod completely into the valve driver body, the position of the valve coupling does not match the position of the valve drive. To remedy the situation: 1. Switch the valve into another position and try again.

  • Page 99: Replacing The Valve Stator

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.2.3 Replacing the Valve Stator Stator for Part No. TitanHP™ column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port valve 6722.9011 6-position, 7-port valve 6722.9031 Suitable for pressures < 345 bar (5000 psi) 2-position, 10-port valve 6722.9021...

  • Page 100: Rotor Seal And Stator Face Seal

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.2.4 Rotor Seal and Stator Face Seal The valve type (standard or biocompatible) and the pH value of the application determine the type of seals: Description Part No. Rotor Seal (RheBuild) kit for TitanHP™ valves For pressures <...

  • Page 101: Replacing The Rotor Seal

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.2.4.1 Replacing the Rotor Seal 1. Remove the stator screws located in the stator (→ Fig. 49, page 91), and then remove the stator from the valve body. 2. Slip a flathead screwdriver underneath the rotor seal and remove the seal.

  • Page 102: Replacing The Main Power Fuses

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.3 Replacing the Main Power Fuses Warning: Before replacing the fuses, turn off the TCC. Be sure to disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez l'instrument. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur.

  • Page 103: Updating The Tcc Firmware

    UltiMate 3000 Series: TCC-3200 Column Compartment 7.4 Updating the TCC Firmware The TCC is shipped with the most recent firmware version. The TCC firmware is also included in Chromeleon. To check which firmware version is installed in the TCC and which version is included in Chromeleon: •...
  • Page 104 If the download was not successful, the related messages appear in the Audit Trail. In this case, repeat the above steps. If this download is also not successful, turn the TCC off and on again, and repeat the download. If the download fails again, contact Dionex Service. Page 96...

  • Page 105: Technical Information

    UltiMate 3000 Series: TCC-3200 Column Compartment 8 Technical Information Temperature range: 5 °C to 85 °C (max. 18 °C below ambient) ±0.5 °C Temperature accuracy: Temperature stability: ±0.1 °C Temperature precision: ±0.1 °C Up to 6 columns, max. 30 cm length...
  • Page 106 UltiMate 3000 Series: TCC-3200 Column Compartment Page 98 Operating Instructions...

  • Page 107: Accessories, Spare Parts, And Consumables

    USB cable (0.5 m, type A to type B, USB 2.0) 6720.8910 6-pin Mini-DIN signal cable (5 m) 6000.1004 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Operating Instructions Page 99...

  • Page 108: Optional Accessories

    UltiMate 3000 Series: TCC-3200 Column Compartment 9.2 Optional Accessories Description Part No. 2 µL pre-column heater (stainless steel) 6722.0530 7 µL pre-column heater (stainless steel) 6722.0540 11 µL pre-column heater (stainless steel) 6722.0550 Drain kit for UltiMate 3000 systems 6040.0005...

  • Page 109: Consumables And Spare Parts

    UltiMate 3000 Series: TCC-3200 Column Compartment 9.3 Consumables and Spare Parts Description Part No.* Column brackets (set of 2 brackets) 6720.0601 Column clip kit (set of 6 clips) 6722.0290 Column ID (set of 5 IDs) 6710.1505 Drain kit for the UltiMate 3000 system 6040.0005...
  • Page 110 6035.9035 USB cable (0.5 m, type A to type B, USB 2.0) 6720.8910 Wrench (size 2.5 mm) 6146.2625 * The part number always refers to the packing unit. For more information, contact your Dionex sales representative. Page 102 Operating Instructions...

  • Page 111: Index

    UltiMate 3000 Series: TCC-3200 Column Compartment 10 Index Connection Chromeleon Computer........25 Digital I/O............. 26 Accessories Drain Tubing ..........48 Optional ............100 Power............25 Standard ............99 USB .............. 25 Consumables ........... 101 Control (Automated) ........53, 57 Back ..............61 Control (Direct) ........... 53, 54 Batch..............58...
  • Page 112 UltiMate 3000 Series: TCC-3200 Column Compartment Rotate............59 Select.............61 Ok..............61 Toggle ............61 Operating Instructions ......... 1 Wash .............59 Operation............51 Fuse Holder ............16 Chromeleon ..........53 Fuse Replacement..........94 Control Elements .......... 14 Display............14 Front Panel Keys .......... 59 Gas Sensor ...........18, 69 Function Key ..........
  • Page 113 UltiMate 3000 Series: TCC-3200 Column Compartment Stator Face Seal ..........92 Status Screen ............. 52 System Wellness..........20 Safety Information ..........2 Safety Precautions ..........3 Safety Symbols ............2 Screen Brightness ..........70 TCC Display..........14, 52 Screen Contrast..........70 Function Key ..........59 Select ..............61...
  • Page 114 UltiMate 3000 Series: TCC-3200 Column Compartment Page 106 Operating Instructions...

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