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Dionex UltiMate FLM-3100 Operating Instructions Manual

Flow managers and thermostatted column compartments
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UltiMate 3000 Series
Thermostatted Column Compartments
Operating Instructions
Flow Managers and
Revision:
Date:
©
2007 Dionex
1.1
September 2007

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Summary of Contents for Dionex UltiMate FLM-3100

  • Page 1 UltiMate 3000 Series Flow Managers and Thermostatted Column Compartments Operating Instructions Revision: Date: September 2007 © 2007 Dionex...
  • Page 2 UltiMate 3000 Series: Flow Managers and Column Thermostats Operating Instructions...
  • Page 3 Flow Manager and Column Compartment Types: FLM-3100, FLM-3200, FLM-3300 FLM-3100B, FLM-3200B, FLM-3300B Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Equipment Directive 73/23/EEC changed by 93/68/EEC • EMC Directive 2004/108/EG...
  • Page 4 UltiMate 3000 Series: Flow Managers and Column Thermostats Page II Operating Instructions...
  • Page 5 UltiMate 3000 Series: Flow Managers and Column Thermostats Table of Contents Introduction........................1 How to Use This Manual ..................1 Safety Information ....................1 1.2.1 Symbols on the Instrument and in the Manual ..........2 1.2.2 General Safety Precautions................3 1.2.3 Consignes Générales de Sécurité...............
  • Page 6 UltiMate 3000 Series: Flow Managers and Column Thermostats User Interface......................41 Power-Up ......................41 Status Screen......................41 Soft Key Menus ....................42 Menu Structure and Layout ................. 43 4.4.1 Main Menu....................... 45 4.4.2 Control Menu....................46 4.4.3 Information Menu .................... 47 4.4.4 Configuration Menu..................

  • Page 7: Introduction

    This document is believed to be complete and accurate at the time of publication. In no event shall Dionex be liable for incidental or consequential damages in connection with or arising from the use of this document.

  • Page 8: Symbols On The Instrument And In The Manual

    UltiMate 3000 Series: Flow Managers and Column Thermostats 1.2.1 Symbols on the Instrument and in the Manual The table below shows the symbols used on the instrument: Symbol Description ˜ Alternating current—Courant alternatif Power supply is on (−) — L'instrument est mis sous tension (−) and Power supply is off (O)—...

  • Page 9: General Safety Precautions

    UltiMate 3000 Series: Flow Managers and Column Thermostats 1.2.2 General Safety Precautions When working with analytical instrumentation, you should know the potential hazards of using chemical solvents. Tips: Before initial operation of the flow manager, make sure that you are familiar with the contents of this manual.
  • Page 10 Disconnect the flow manager from all power sources before removing the panels. Remove panels only when instructed to do so in these instructions. • Always replace blown fuses with the original spare fuses from Dionex (→ Replacing the Fuses, page 73).

  • Page 11: Consignes Générales De Sécurité

    • Before interrupting operation for several days or more, observe the precautions in Shutting Down the Flow Manager (→ page 81). • Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex accessories may impair the performance of the instrument.
  • Page 12 électrique avant de retirer les capots. Ne démontez les capots de protection que si cela est explicitement demandé au cours de ces instructions. • Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex (→ Replacing the Fuses, page 73).
  • Page 13 Shutting Down the Flow Manager (→ page 81). • Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument.

  • Page 14: Intended Use

    Buffer concentration: typically up to 1 mol/L (0.1 mol/L if chloride ions are present). If there is a question regarding appropriate usage, contact Dionex before proceeding. Dionex is not liable for any damage, material or otherwise, resulting from inappropriate or improper use of the instrument.

  • Page 15: Overview

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2 Overview 2.1 Product Overview The flow manager is a reliable module of the UltiMate 3000 system and performs equally well as a dependable system for routine analyses or as a sophisticated research instrument for nano, capillary, and micro HPLC applications.
  • Page 16 UltiMate 3000 Series: Flow Managers and Column Thermostats • Depending on the configuration, the flow manager is equipped with one or two column switching valves (→ Supported Configurations, page 12), e.g., for applications that require different columns at frequent intervals at similar temperatures. •...

  • Page 17: Theory Of Operation (Ultiflow)

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.2 Theory of Operation (UltiFlow) The fundamental requirement on a flow manager for liquid chromatography is the ability to create constant low flow rates for nano, capillary, and micro HPLC applications, as well as to equalize the temperature.

  • Page 18: Supported Configurations

    2.3 Supported Configurations 2.3.1 General For an overview of the currently available models, refer to the list below. If you have any questions, do not hesitate to contact your Dionex sales representative or distributor. Application Description Part No.

  • Page 19: Biocompatible Flow Managers

    For information about how to replace a cartridge, refer to section 7.6 (→ page 76). For information about the switching valve, refer to section 2.5 (→ page 18). If you have any questions, do not hesitate to contact your Dionex sales representative or distributor.
  • Page 20 UltiMate 3000 Series: Flow Managers and Column Thermostats Follow the steps below to connect the components to the biocompatible switching valve. The description refers to the connection of a nano column, but applies to the other components, as well. 1. To establish a zero dead volume fitting connection between the column and the switching valve, a finger-tight nut, ferrule, and sleeve are required.
  • Page 21 Tip: Dionex cannot provide a torque specification because the force required to tighten the nut can vary due to the friction between nut and the thread, as well as the composition and wall thickness of the component to be connected.
  • Page 22 UltiMate 3000 Series: Flow Managers and Column Thermostats 9. Pull the column back (→ Fig. 8) and install the preassembled fitting with the column to the appropriate port on the switching valve. Make certain that the column does not extend the sleeve when installing it into the port Fig.

  • Page 23: General Flow Manager Design

    Locking mechanism for the front panel door (→ section 3.5, page 28) Important: Use only the capillaries shipped with the flow manager and original spare capillaries from Dionex. Install the capillaries and fittings only at the positions for which they are intended.

  • Page 24: Column Switching Valve

    UltiMate 3000 Series: Flow Managers and Column Thermostats Important: Utilisez uniquement les capillaires fournis avec l'instrument et les capillaires de rechange d'origine Dionex. Installez les capillaires et les raccords uniquement dans les positions pour lesquelles ils sont prévus. La réutilisation des raccords et ferrules n’est possible que pour la connexion capillaire d’origine, afin d’éviter l’apparition de volumes...

  • Page 25: Flow Splitter

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.6 Flow Splitter The flow manager is equipped with a flow splitter (→ Fig. 11) that delivers the low flow rates required for nano, capillary, and micro HPLC applications. Splitter cartridges with different split ratios are available for the different applications.

  • Page 26: Front Panel Display And Controls

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.7 Front Panel Display and Controls Fig. 12: Front panel Front Panel Element Description Display Shows information about the flow manager, e.g.: - General information upon power-up (→ section 4.1, page 41) - Status screen (→...

  • Page 27: Rear Panel Connectors

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.8 Rear Panel Connectors Fig. 13: Rear panel connectors Description Power switch Fuse holder (→ section 7.3, page 72) Main power receptacle Type label Digital I/O for communication with external devices (→ section 3.4.3, page 28) USB (Universal Serial Bus Interface) port for connection to the server PC (→...

  • Page 28: Leak Sensors

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.9 Leak Sensors The flow manager contains two leak sensors to detect any humidity or gases that may accumulate in the column chamber. When a certain humidity or gas concentration is reached in the chamber (while the door is closed), the corresponding sensor is activated. Eliminate the cause for the leakage, wearing the appropriate protective clothing, and ventilate the inside before closing the door.

  • Page 29: System Wellness

    UltiMate 3000 Series: Flow Managers and Column Thermostats 2.10 System Wellness The flow manager supports several system wellness and reliability features that can help you detect small problems before they turn into big ones: • Leak detector (→ page 21) •...
  • Page 30 UltiMate 3000 Series: Flow Managers and Column Thermostats Page 24 Operating Instructions...

  • Page 31: Installation

    Tips: Immediately report any shipping damage to both the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately. Keep the original shipping container and packing material. They provide excellent protection for the flow manager in case of future transit.

  • Page 32: Positioning The Flow Manager In The Ultimate 3000 System

    3.3 Positioning the Flow Manager in the UltiMate 3000 System As the flow manager is part of an UltiMate 3000 system, Dionex recommends that you stack the individual modules, for example, as shown in Fig. 14. However, the arrangement of the system modules depends on the application.

  • Page 33: Connecting The Flow Manager

    Data is transferred digitally via the appropriate USB cable (part no. 6911.0002, USB extension cable, part no. 6000.1005). To ensure trouble-free operation, all USB cables (see above) should be ordered from Dionex. Tip: If you want to operate the flow manager with Chromeleon, install the Chromeleon software before connecting the flow manager to the USB port on the Chromeleon server PC.

  • Page 34: Digital I/O

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3.4.3 Digital I/O The two 6-pin Mini-DIN (→ Fig. 13, no. 5) ports can be used in Chromeleon to exchange digital signals with external instruments. The accessories kit includes a 6-core Mini-DIN signal cable (part no.

  • Page 35: Preparing The Flow Manager

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3.6 Preparing the Flow Manager Important: The flow manager is filled with a mixture of isopropanol and water (20:80) when being shipped from the factory. During initial operation, make sure that the solvents used are miscible. Otherwise, use an appropriate intermediate solvent.
  • Page 36 Dionex recommends connecting, for example, PTFE tubing with an inner diameter of 1 mm (minimum 0.5 mm). Make sure that the tubing is long enough to reach the bottom of the waste container.

  • Page 37: Installing A Separation Column

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3. Install the column(s) as described in Installing a Separation Column (→ page 31). 4. Install the column(s) as described in Installing a Trap Column (→ page 36). 5. Establish the fluid connections between the column(s), switching valve(s), and flow splitter according to your application.
  • Page 38 UltiMate 3000 Series: Flow Managers and Column Thermostats Press the ring in the direction indicated by the arrows on the ring. Fig. 19: Two-piece column clip 2. When installing the column clips for the first time: the kit includes self-adhesive foam pads to protect the column.
  • Page 39 Capillary guide Fig. 22: Capillary guide installed on the carrier Dionex recommends guiding the capillaries out of the interior in such a way that the connections are as short as possible. Operating Instructions...
  • Page 40 UltiMate 3000 Series: Flow Managers and Column Thermostats Tip: Place the capillaries in such a manner (i.e., preferably 90-degree angle related to the door seal) that they do not open a small path for ambient air into the column chamber. This will prevent a proper seal and thus, impair the heating and cooling performance of the flow manager.

  • Page 41: Connecting The Capillaries To The Column Switching Valve

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3.6.2 Connecting the Capillaries to the Column Switching Valve Depending on the instrument configuration, the flow manager is fitted with one or two column switching valves (2-position/10-port or 2-position/6-port). Valve switching is programmed and controlled via Chromeleon.

  • Page 42: Installing A Trap Column

    UltiMate 3000 Series: Flow Managers and Column Thermostats Important: To connect the capillaries to the switching valve, install only the ferrules and fitting screws provided in the flow manager's accessories kit. Important: Pour raccorder les capillaires à la vanne d'injection, installez uniquement les ferrules et les raccords fournis dans le kit d'accessoires.

  • Page 43: Connecting The Flow Splitter

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3.6.4 Connecting the Flow Splitter Important: The flow sensor connectors (→ Fig. 11, no. 2) and the flow control valve connector (→ Fig. 11, no. 3) are installed in the factory. They should not be opened by the user.
  • Page 44 UltiMate 3000 Series: Flow Managers and Column Thermostats 1. Use the special capillary that is shipped with the Application Kit to connect the flow manager to the pump (→ Fig. 11, page 19). Description Part No. Long capillary for connecting the pump to the flow splitter (including the appropriate fittings and ferrules) Standard version 6035.2550...

  • Page 45: Manual Injection Port

    UltiMate 3000 Series: Flow Managers and Column Thermostats 3.7 Manual Injection Port A manual injection port (→ Fig. 25) is available from Dionex as an option for the flow manager: Part No. Description 6720.9007 Manual Injection Port, standard flow manager 6721.9007...
  • Page 46 UltiMate 3000 Series: Flow Managers and Column Thermostats Page 40 Operating Instructions...

  • Page 47: User Interface

    UltiMate 3000 Series: Flow Managers and Column Thermostats 4 User Interface 4.1 Power-Up Use the main power switch on the rear panel to turn the flow manager on and off. The device type, serial number and firmware version are displayed on the display. Tips: The noise that is heard for a few seconds after turning on the flow manager is normal and does not impair the flow manager's performance.

  • Page 48: Soft Key Menus

    To show the soft key menus, select the position of a white dot on the front panel display with the Dionex menu pen. The menu pen (part no. 6300.0100) is included in the accessories kit.

  • Page 49: Menu Structure And Layout

    UltiMate 3000 Series: Flow Managers and Column Thermostats Tips: If no option is selected, the original status screen line is restored after about 5 seconds. When the flow manager is connected in Chromeleon, front panel input related to the measurement is disabled to prevent changes to the operating parameters.
  • Page 50 UltiMate 3000 Series: Flow Managers and Column Thermostats In general, the menu layout is as follows: > Control (1..3) 1. Temperature control 2. Valve control 3. Leak control ∨ ∧ Select Back Fig. 30: Menu layout (here: Control menu) Description Reports the menu name and the number of items on the menu list.

  • Page 51: Main Menu

    UltiMate 3000 Series: Flow Managers and Column Thermostats Tip: If an error is found, one or more error messages appear on the front panel display. In this case, the following soft keys appear on the navigation bar: Soft Key Description Prev Returns you to the previous error message.

  • Page 52: Control Menu

    UltiMate 3000 Series: Flow Managers and Column Thermostats 4.4.2 Control Menu On the Control menu, you can make the temperature and valve settings. In addition, you can determine the behavior for a leak alarm: > Control (1..3) 1. Temperature control 2.

  • Page 53: Information Menu

    UltiMate 3000 Series: Flow Managers and Column Thermostats 4.4.3 Information Menu The Information menu provides general information about the flow manager, e.g., for diagnostic purposes: > Information (1..3) 1. System 2. Valve 3. Temperature ∨ ∧ Select Back Fig. 33: Information menu Menu Item Description System...

  • Page 54: Configuration Menu

    Fig. 34: Configuration menu Menu Item Description Reset to factory defaults Select to restore the Dionex standard settings. The Reset to factory defaults? dialog is opened. To confirm the restore, select OK. Else, select Cancel. Display & Soft keys Sets the display and soft key parameters: Brightness—...

  • Page 55: Software Control By Chromeleon

    5.2 Installing the Flow Manager in Chromeleon Tip: Dionex recommends installing the Chromeleon software before connecting the flow manager to the USB port on the Chromeleon computer. When you install the Chromeleon software first, the Windows operating system will detect the flow manager automatically when the power is turned on.
  • Page 56 10. On the Edit menu, click Add Device or right-click and click the command on the context menu. 11. The Add device to timebase dialog box is opened. Select Dionex HPLC: UltiMate 3000 from the left list box, and then select FLM-3000 Flow Manager from the right list box.
  • Page 57 UltiMate 3000 Series: Flow Managers and Column Thermostats General Wizard Page Define the general instrument parameters. Fig. 35: General wizard page • The Device Name field reports the name under which the flow manager is identified in the installation environment and in the Chromeleon client. Accept the default device name (ColumnOven).
  • Page 58 Audit Trail. In this case, turn off the flow manager. Turn on the flow manager again and repeat the download as described above. If the download fails again, contact Dionex Service.. Important: Au cours du téléchargement, assurez-vous que la communication entre l'instrument et Chromeleon n'est pas interrompue et n'arrêtez...
  • Page 59 UltiMate 3000 Series: Flow Managers and Column Thermostats Components Wizard Page Specify which other components are installed: Fig. 36: Components wizard page Option Description FLM-3100 Indicate which flow manager is part of your system. FLM-3200 FLM-3300 ChipCard A type The flow manager is fitted with a column identification system (→ section 5.5, ( B, C, D type, resp.) page 58) and a flow splitter identification system (→...
  • Page 60 UltiMate 3000 Series: Flow Managers and Column Thermostats Tip: To change the basic settings you may as well reopen these configuration pages after the installation. Select FLM-3x00 Flow Manager in the Chromeleon Server Configuration program, and then select Properties on the context menu.
  • Page 61 Operational Qualification or running a Flow Control Test for the flow manager. In addition, some of the recorded values provide valuable information to Dionex Service for troubleshooting in case the flow manager does not pass Operational Qualification successfully.

  • Page 62: Assigning The Flow Splitter

    In the standard configuration of the UltiMate 3000 system with a DGP-3600M, Dionex recommends assigning the flow splitter to the right pump (MicroPump). Page 56...

  • Page 63: Operating The Flow Manager With Chromeleon

    (For details, refer to the Chromeleon online Help.) Operational and/or Performance Qualification allows you to check and document the quality of your HPLC system. All required materials and detailed instructions are available from Dionex on request. Operating Instructions Page 57...

  • Page 64: Column Identification System (Column Id)

    The column is shipped with the chip card attached. 1. Insert the chip card in one of the four memory card slots (→ Fig. 39). Make sure that the Dionex logo faces upward. Fig. 39: Inserting a memory chip card in a memory card slot...

  • Page 65: Splitter Identification System (Splitter Id)

    1. Insert the chip card (with the Dionex logo facing up; → Fig. 39, page 58) in the second memory card slot from the right. As standard, this slot is for the splitter ID chip card.

  • Page 66: Diagnostics

    UltiMate 3000 Series: Flow Managers and Column Thermostats 5.7 Diagnostics With Chromeleon 6.80 service pack 2, Chromeleon supports Diagnostics functions for the flow manager. These tests allow you to check the performance of certain device components. For information about how to run the tests, refer to the Chromeleon online Help.

  • Page 67: Troubleshooting

    60). If a test fails, check the Chromeleon Diagnostics Messages section (→ page 66) for a short description of possible causes along with recommended courses of action. If you are unable to eliminate a problem following the instructions given here, contact Dionex Service.

  • Page 68: Error Messages

    The table below lists flow manager-related error messages along with possible causes and suggests appropriate remedial actions. In addition to the messages below, other messages may appear. In this case, please note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem.
  • Page 69 UltiMate 3000 Series: Flow Managers and Column Thermostats Message Probable Cause Remedial Action Error while programming An error occured while the flash Turn the flow manager off and flash! memory was programmed. on again. (This message may be extended by additional text.) Flash object error! An error occured in the object Turn the flow manager off and...
  • Page 70 The left switching valve is Retry installed but cannot be accessed. Left MSV error! An error has been reported for Contact Dionex Service if the the left switching valve. messages appears reapeatedly. Left MSV error! The left switching valve cannot Switching is repeated Retrying…...
  • Page 71 UltiMate 3000 Series: Flow Managers and Column Thermostats If communication between Chromeleon and the flow manager cannot be established, related error messages may appear in the Chromeleon audit trail. Refer to the table below for the most important error messages, along with possible causes and the corresponding remedial actions.

  • Page 72: Chromeleon Diagnostics Messages

    UltiMate 3000 Series: Flow Managers and Column Thermostats 6.3 Chromeleon Diagnostics Messages If the flow manager fails a diagnostics test, perform the instructions given here. If the detector still fails the test, contact Dionex Service. Flow Control Test failed Test Result...
  • Page 73 UltiMate 3000 Series: Flow Managers and Column Thermostats Test Result Possible Cause Remedial Action Flow = %. Wrong flow The blind nut leaks. Check the blind nut at the splitter direction. outlet for leakage. The flow sensor is not connected or Turn FLM off and on.
  • Page 74 UltiMate 3000 Series: Flow Managers and Column Thermostats Gas Sensor Test failed Test Result Possible Cause Remedial Action Error gas leak detected! The gas sensor detected an Eliminate the cause. Tighten increased concentration of gas in leaking connection and vent the the column chamber.

  • Page 75: Routine Maintenance

    The following sections describe all maintenance procedures that can be carried out by the user. Dionex personnel should perform any additional servicing, as well as annual inspections to detect contamination, wear, etc. If unexpected problems occur, please contact Dionex Service.
  • Page 76 Utilisez les pièces de rechange d'origine Dionex. Effectuer des remplacements avec des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument, rendant ainsi caduque la garantie de produit. Référez-vous à la déclaration de garantie dans les conditions générales de vente pour plus d'information.

  • Page 77: Maintenance Intervals

    For information about maintenance procedures for the column switching valve, refer to section 10 (→ page 91). All other maintenance procedures must be performed by Dionex Service personnel. Have a Dionex service representative check the flow manager once a year to prevent contamination, excessive wear, etc.

  • Page 78: Flow Control Valve

    UltiMate 3000 Series: Flow Managers and Column Thermostats 7.3 Flow Control Valve The flow manager uses a Flow-Control Valve (FCV) installed in the rear of the enclosure (on the right) to control the column flow. Minute particles that may be contained in the solvents or be caused by the unavoidable abrasion of the pump seals may cause damage to the flow control valve.

  • Page 79: Replacing The Fuses

    UltiMate 3000 Series: Flow Managers and Column Thermostats 7.4 Replacing the Fuses Warning: Before replacing the fuses, turn off the flow manager. Be sure to disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez l'instrument. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur.

  • Page 80: Drying The Fluid Leak Sensor

    UltiMate 3000 Series: Flow Managers and Column Thermostats 7.5 Drying the Fluid Leak Sensor Tips: Inspect the connections for signs of leakage every day and tighten leaking connections. Important: Also, inspect the waste port and waste tubing for indications of blockage. If the waste tubing is blocked or bent, the waste cannot run out of the instrument, causing a leak alarm.
  • Page 81 UltiMate 3000 Series: Flow Managers and Column Thermostats 2. Push and remove the side panel backward, toward the rear panel (→ Fig. 42). Remove the side panel in this direction. Fig. 42: Removing the side panel towards the rear panel 3.
  • Page 82 UltiMate 3000 Series: Flow Managers and Column Thermostats 7.6 Exchanging the Splitter Cartridge) The following splitter cartridges are available from Dionex: Part No. Description Standard Flow Managers: 6720.3150A Splitter cartridge for a split ratio of 1:1000 6720.3155A Splitter cartridge for a split ratio of 1:300 6720.3160A...
  • Page 83 UltiMate 3000 Series: Flow Managers and Column Thermostats To change the splitter cartridge: 1. Disconnect the capillary on the flow splitter's pump connector (→ Fig. 11, page 19). 2. Loosen the fittings on the splitter cartridge outlets (→ Fig. 44). Fig.
  • Page 84 UltiMate 3000 Series: Flow Managers and Column Thermostats 8. Flush the splitter to remove any particles that may have polluted the splitter during the exchange of the cartridge. To do so, place a lint-free cloth under the splitter to absorb the solvent that leaks out (→...

  • Page 85: Column Switching Valve

    UltiMate 3000 Series: Flow Managers and Column Thermostats 7.7 Column Switching Valve Maintenance requirements are kept to a minimum. In most instances, it will be sufficient to clean the valve by flushing all lines with an appropriate solvent. The nature of the solvent to be used depends on the sample(s) and the mobile phase(s) that are used.
  • Page 86 UltiMate 3000 Series: Flow Managers and Column Thermostats 2. To ensure that the sealing surface of the stator cap is not damaged, rest the stator on its outer face. If the tubing is still attached, leave it suspended by the tubing. 3.

  • Page 87: Shutting Down The Flow Manager

    In case buffers (phosphate) or salt containing solutions are used, flush the system and column for 30 minutes with a salt-free solution first. In this case, Dionex recommends flushing the system before overnight inactivity also. Ship the unit only in the original shipping container and observe the packing instructions (→...
  • Page 88 UltiMate 3000 Series: Flow Managers and Column Thermostats Long term interruptions of over a week: To shut down the flow manager: 1. Flush the column(s) according to manufacturer recommendations. 2. Prime all lines with an eluent containing more than 40% isopropanol. 3.

  • Page 89: Technical Information

    UltiMate 3000 Series: Flow Managers and Column Thermostats 8 Technical Information Temperature range: 5–70 °C (max. 15 °C below ambient) ±0.5 °C Temperature accuracy: Temperature stability: ±0.1 °C Temperature precision: ±0.1 °C Column capacity: Up to 3 columns, max. 30-cm length From 20 to 50 °C in less than 25 min Heatup time: (at 23 °C ambient temperature, ±0.5 °C)
  • Page 90 UltiMate 3000 Series: Flow Managers and Column Thermostats GLP features: In Chromeleon: Full support of automatic equipment qualification (AutoQ) and System Wellness monitoring All system parameters are recorded in the Chromeleon audit trail. 162 VA Power requirements: Automatic voltage selection Range of use: Indoor use Environmental...

  • Page 91: Accessories, Consumables And Spare Parts

    Included in 6710.1505 Flow Control Valve Maintenance Kit 6720.3195 Dionex menu pen 6300.0100 Assortment box for accessories 6000.0043 * The part number always refers to the packing unit. For more information, please contact your Dionex sales representative. Operating Instructions Page 85...

  • Page 92: Consumables, Spare Parts, And Optional Accessories

    UltiMate 3000 Series: Flow Managers and Column Thermostats 9.2 Consumables, Spare Parts, and Optional Accessories Description Part No.* Adaptor from capillary column to flow manager switching valve (standard valve) 6720.0040 (PeekSil, 50 µm ID, 10 cm long, 1/16") Adaptor from capillary column to flow manager switching valve (biocompatible 6721.0040 valve) (PeekSil, 50 µm ID, 10 cm long, 1/16")
  • Page 93 UltiMate 3000 Series: Flow Managers and Column Thermostats Description Part No.* Capillary from capillary column to (biocompatible) autosampler 6721.0025 (50 µm ID x 50 cm, PeekSil) Capillary from flow splitter and flow manager switching valve 6720.0033 (20 µm ID x 30 cm, PeekSil) for nano HPLC applications, standard device Capillary from flow splitter and flow manager switching valve 6721.0033 (20 µm ID x 30 cm, PeekSil) for nano HPLC applications, biocompatible device...
  • Page 94 Micro flow waste container 6720.0011 Nut (1/16", 19 mm long, SS) (set of 10) 6720.0019 Nut (1/16", L=5", SS) (set of 10; recommended by Dionex) 6720.0020 Nut (1/16", standard, SS) (set of 10) 6720.0018 PEEK tubing (130 µm I.D. x 75 cm) including appropriate fittings 6720.3220...
  • Page 95 UltiMate 3000 Series: Flow Managers and Column Thermostats Description Part No.* PEEKsil bridge (standard) between left and right flow manager switching valves 6720.0060 (75 µm ID, 50 cm long), including appropriate fittings PEEKsil bridge (biocompatible) between left and right flow manager switching valves 6721.0060 (75 µm ID, 50 cm long), including appropriate fittings PEEKsil bridge (standard) between left and right switching valves in flow manager...
  • Page 96 Wrench (size 3.0 mm) 6000.0050 Wrench, open-end (1/4" x 5/16") 6000.0051 Wrench, box wrench (slotted head, size 1/4") 6000.0052 * The part number always refers to the packing unit. For more information, please contact your Dionex sales representative. Page 90 Operating Instructions...

  • Page 97: Technical Appendix - Pin Assignment

    UltiMate 3000 Series: Flow Managers and Column Thermostats 10 Technical Appendix - Pin Assignment Two 6-pin Mini-DIN ports on the instrument's rear panel (→ Fig. 13, page 21) can be used in Chromeleon to exchange digital signals with external instruments. Relay_Com Relay_NO* Digital Input...
  • Page 98 UltiMate 3000 Series: Flow Managers and Column Thermostats Page 92 Operating Instructions...

  • Page 99: Index

    UltiMate 3000 Series: Flow Managers and Column Thermostats 11 Index Accessories Error Levels (Dialog Box)......... 57 Optional ............92 Error Messages ..........66, 67 Standard ............91 Automated Control ..........51 Facility Requirements........26 FCC Note............. 8 Back ..............46 Firmware Download.......... 54 Biocompatible Flow Managers......13 Flow Control Valve ........
  • Page 100 UltiMate 3000 Series: Flow Managers and Column Thermostats Main Menu ............47 Safety Information..........1 Maintenance............75 Safety Precautions ..........3 Column Switching Valve ......85 Safety Symbols............ 2 Flow Control Valve........78 Select ..............46 Fluid Leak Sensor .........80 Separation Column Installation ......32 Fuse Replacement .........79 Shut-Down ............
  • Page 101 UltiMate 3000 Series: Flow Managers and Column Thermostats Valve..............49 Warnings ............. 2 Column Switching Valve ......85 Wellness ............24 Flow Control Valve........78 Windows ............52 Valve Control ............48 Operating Instructions Page 95...

This manual is also suitable for:

Ultimate flm-3300Ultimate flm-3200Ultimate flm-3100bUltimate flm-3300bUltimate flm-3200bUltimate 3000 series

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