Dionex UltiMate 3000 Series Operating Instructions Manual
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Dionex UltiMate 3000 Series Operating Instructions Manual

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UltiMate 3000 Series
SRD-3x00 Solvent Racks
Operating Instructions
(Original Operating Instructions)
Revision: 1.5
Date: September 2010
© 2010 Dionex
Doc. No. 4820.3550

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Hyunbin
March 21, 2025

Ultimate 3000 {PUMP} Upper pressure limit exceeded. Alarm keep open. What should I do?

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Hyunbin
March 21, 2025

@Hyunbin Am I doing something wrong with my settings? Or is it a pump problem?

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Summary of Contents for Dionex UltiMate 3000 Series

  • Page 1 UltiMate 3000 Series SRD-3x00 Solvent Racks Operating Instructions (Original Operating Instructions) Revision: 1.5 Date: September 2010 © 2010 Dionex Doc. No. 4820.3550...
  • Page 2 UltiMate 3000 Series: SRD-3x00 Solvent Racks Operating Instructions...
  • Page 3 Product: Solvent Rack Types: SR-3000, SRD-3200, SRD-3400, and SRD-3600 Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations:  Machinery Directive 2006/42/EC  Low-Voltage Directive 2006/95/EC  EMC Directive 2004/108/EC The safety of the machinery was evaluated based on the following standard: ...
  • Page 4 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: SRD-3x00 Solvent Racks Table of Content Introduction........................1 How to Use this Manual ..................1 Safety Information ....................2 1.2.1 Symbols on the Instrument and in the Manual ..........2 1.2.2 General Safety Precautions................3 1.2.3 Consignes Générales de Sécurité..............4 Intended Use ......................
  • Page 6 UltiMate 3000 Series: SRD-3x00 Solvent Racks Operational Settings.....................30 5.3.1 Turning the Degasser on or off (from the UltiMate 3000 System Pump) ..30 5.3.1.1 Turning the Degasser on and off from the Pump Display .......30 5.3.1.2 Turning the Degasser on and off in Chromeleon ........31 5.3.2...

  • Page 7: Introduction

    The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of the Solvent Rack, Dionex recommends that you review the manual thoroughly before beginning operation.

  • Page 8: Safety Information

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 1.2 Safety Information The CE Mark label and cTUVus Mark safety label on the instrument indicate that the instrument is compliant with the related standards. 1.2.1 Symbols on the Instrument and in the Manual...

  • Page 9: General Safety Precautions

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 1.2.2 General Safety Precautions When working with analytical instrumentation, you should know the potential hazards of using chemical solvents. Tips: Before initial operation of the SRD, make sure that you are familiar with the contents of this manual.

  • Page 10: Consignes Générales De Sécurité

    Before interrupting operation for several days or more, observe the precautions on page 34.  Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex accessories may impair the performance of the SRD.  Do not use the SRD in ways other than those described in these operating instructions.
  • Page 11 UltiMate 3000 Series: SRD-3x00 Solvent Racks  Pour raison de sécurité, ne soulevez pas l'instrument avec des réservoirs.  Ne placez aucun objet lourd sur la porte ouverte du panneau avant. Ceci pourrait endommager la porte.  Réglez toujours une limite de pression minimum pour le système HPLC. Ceci prévient les dommages résultant de fuites ou de long-terme fonctionnement à...
  • Page 12 34.  Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument. ...

  • Page 13: Intended Use

    HPLC (high performance liquid chromatography) solvents. The SRD is controlled by the Chromeleon Chromatography Management System from the HPLC pump of the UltiMate 3000 series. However, the SRD can be used with other HPLC systems if adequate control inputs and outputs are available.
  • Page 14 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 8 Operating Instructions...

  • Page 15: Overview

    HPG-3400 two HPG-3200 pumps in a two-stack system one HPG-3200 or ISO-3100 if you want to degas the solvents and the wash liquid of an UltiMate 3000 series autosampler one NCS-3500RS SRD-3200 SRD with analytical 2-channel vacuum degasser 5035.9250...

  • Page 16: Front Panel Elements

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.3 Front Panel Elements Fig. 1: Front panel view Front Panel Description Element LEDs The LED is blue when the SRD power is on. Power Vacuum The LED is green if the degasser is working properly.

  • Page 17: Rear Panel

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.4 Rear Panel Fig. 2: Rear panel view Description 15-pin D-Sub port: Connects the SRD to the pump ( page 19) DC Input (Power) Only to be used if the SRD is not controlled by the UltiMate 3000 system pump.

  • Page 18: Fluid Connections

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.5 Fluid Connections The front panel door tilts upward to provide easy access to the fluid connections in the SRD. The open door locks in its topmost position. Fig. 3: SRD with open front panel door (here SRD-3600)

  • Page 19: Leak Sensor

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.6 Leak Sensor A leak sensor is installed inside the SRD. If liquid collects in the drip tray under the fluid connections, the leak sensor reports a leak, and the Status LED on the front panel door changes to red.
  • Page 20 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 14 Operating Instructions...

  • Page 21: Installation

    Tips: Immediately report any shipping damage to both, the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately. Keep the original shipping container and packing material. They will provide excellent protection for the SRD in case of future transit.
  • Page 22 UltiMate 3000 Series: SRD-3x00 Solvent Racks 3. Remove the foam spacers, and then remove the polythene packaging. 4. Before connecting the SRD to the power source, wait approximately 4 hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate.

  • Page 23: Positioning The Srd In The Ultimate 3000 System

    If the SRD is part of an UltiMate 3000 system, for example, for analytical HPLC applications, Dionex recommends that you stack the individual modules, for example, as shown in Fig. 4 and interconnecting them on the rear panel as shown in Fig. 5. However, the arrangement of the system modules depends on the application.
  • Page 24 Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument. However, Dionex recommends interconnecting all modules, and then connecting the system to the Chromeleon computer by using only one connection. For systems with a DAD-3000(RS) or MWD-3000(RS), you can use only the hub on the detector for the connection.

  • Page 25: Connecting The Srd

    UltiMate 3000 system pump. For this scenario, an external power supply (part no. 6510.0004) is available from Dionex as an option for the SRD. Use the external power supply unit to connect the DC input on the rear panel of the SRD to a power source that is connected to a true ground.
  • Page 26 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 20 Operating Instructions...

  • Page 27: Preparation For Operation (Startup)

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 4 Preparation for Operation (Startup) 4.1 Overview After you have unpacked, positioned, and connected the SRD described in sections 3.1 through 3.4 ( page 15 and following pages), prepare the SRD for operation: 1. Connect drain tubing ( section 4.2).

  • Page 28: Solvent Reservoirs

    (for example, isopropanol) to replace them step-by- step. Tip: If the UltiMate 3000 system includes an UltiMate 3000 series autosampler, Dionex recommends degassing the wash liquid on a continuous basis, for example, by using the degasser of an appropriate SRD. The procedure how to prepare and install the wash liquid lines is similar to the connecting the solvent lines.

  • Page 29: Connecting The Solvent Reservoirs

    4. If required Cut the tubing straight if necessary. The end of the solvent line should be cut straight and not deformed. Use only the original Dionex solvent lines. 5. Place the entire assembly in the solvent reservoir. 6. Tighten the reservoir cap hand-tight. Press the retaining guide into the hole in the reservoir cap to hold the solvent line in place inside the reservoir.
  • Page 30 UltiMate 3000 Series: SRD-3x00 Solvent Racks 8. Remove the blind fitting from the degasser inlet if necessary and connect the solvent line with the associated line label. Degasser inlets (solvent line connection) Degasser outlets (to pump) Tubing guides Fig. 9: Example for degasser connections (here SRD-3600)

  • Page 31: Connecting The Solvent Lines From The Pump To The Degasser

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 4.3.3 Connecting the Solvent Lines from the Pump to the Degasser With all UltiMate 3000 system pumps, the solvent supply lines are connected to the pump at the factory. 1. If necessary, route the solvent lines through the opening between the front panel door and top cover (...
  • Page 32 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 26 Operating Instructions...

  • Page 33: Operation And Maintenance

    < 8014538, you should avoid also hexanes (60% n-Hexane).  The UltiMate 3000 series pumps are shipped with reversed phase piston seals (RP). Keep in mind that using chloroform, trichlorobenzene, methylene chloride, tetrahydrofuran, or toluene as solvents chemically damages the UHMW-PE seals.
  • Page 34 After operation, rinse out buffers and solutions that form peroxides. Important: Do not recycle the solvent or use methanol from aluminum reservoirs. This may impair the performance of the seals. Important: Dionex déconseille de recycler les solvants. Ceci peut nuire aux performances des joints. Page 28 Operating Instructions...

  • Page 35: General Notes For Degasser Operation

    In addition, observe the following to ensure optimum degassing performance:  Dionex recommends filling all channels with eluent and degassing all channels (even if they are not used for the application). This will reduce the speed of the vacuum pump and reduce the degasser noise.

  • Page 36: Operational Settings

    Standby mode  Dionex recommends always leaving the degasser on. 5.3.1.1 Turning the Degasser on and off from the Pump Display 1. On the front panel display of the pump, show the function keys and select Menu. (For details, see the Pump Manual and/or NCS/NCP Manual.)

  • Page 37: Turning The Degasser On And Off In Chromeleon

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.3.1.2 Turning the Degasser on and off in Chromeleon If the pump is operated with Chromeleon, select one of the following alternatives to turn the degasser on and off:  Directly from the Panel Tabset ( page 31) ...

  • Page 38: Turning The Degasser On Or Off (Stand-Alone Operation)

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 3. Verify that the module (pump, NCS, or NCP) is connected to Chromeleon (the LED next to the Connect button is green). If it is not, click Connect. 4. Click More Options and select On or Off under Degasser Mode.

  • Page 39: Special Chromeleon Functions

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.4 Special Chromeleon Functions This section provides a short overview of some special functions that Chromeleon supports for the SRD. These functions are available in the Commands dialog box for the pump or on the control panel for the pump. For more information, see the Chromeleon Help.

  • Page 40: Shutting Down The Srd

     Before returning an SRD to Dionex for repair, contact Dionex Service or your local distributor. An RMA (Return Material Authorization) number is required in order to track your instrument. Always use the original packaging and observe the packing instructions when shipping the SRD.

  • Page 41: Routine And Preventive Maintenance

    Check the volume of the liquid in the waste container and empty as needed. Annually Have a Dionex service representative check the detector once a year to prevent contamination and excessive wear. Tip: If the SRD is operated with the pump of an UltiMate 3000 system and if the pump is operated by Chromeleon, you can check the performance of the SRD degasser (...
  • Page 42 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 36 Operating Instructions...

  • Page 43: Troubleshooting

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the SRD or UltiMate 3000 system. Status Indicators The status indicators on the front panel display provide a quick visual check of the operational status of the SRD.

  • Page 44: Messages On The Pump Display

    The table lists SRD-related messages along with recommended remedial actions. In addition to the messages in the table, other messages may appear. In this case, note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem. Message Remedial Action Degasser malfunction.

  • Page 45: Operating Problems

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 6.4 Operating Problems The following table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information, also see the manuals for the other modules of an UltiMate 3000 system.
  • Page 46 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action High baseline drift The flow cell is dirty. Clean the flow cell. Replace the (Cont'd) flow cell if necessary ( Detector Manual). The lamp is too old. Replace the detector lamp (...
  • Page 47 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action Poor reproducibility There are air bubbles in the pump Purge the pump; check general (Cont'd) heads and/or flow meter. function ( Pump Manual, NCS/NCP Manual). NCS-3500RS, NCP-3200RS Purge the pump ( NCS/NCP After changing solvents, the Manual).
  • Page 48 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action Reproducible ghost peaks Contamination occurs somewhere Flush the system using an in the chromatogram. in the system. appropriate solvent. (Cont'd) Degasser noise The vacuum pump of the degasser Fill and degas all channels is running at high speed.

  • Page 49: Service

     Before returning an SRD to Dionex for repair, contact Dionex Service or your local distributor. An RMA (Return Material Authorization) number is required in order to track your instrument. Always use the original packaging and observe the packing instructions when shipping the SRD.

  • Page 50: Rinsing The Degassing Channels

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 7.2 Rinsing the Degassing Channels To avoid contamination of the degasser, you should prepare fresh solvents, clean the solvent lines, and rinse the degassing channels at regular intervals. Rinsing the degassing channels is especially important for the channel that degasses aqueous solvents (for reverse-phase chromatography).

  • Page 51: Eliminating Leakage In The Solvent Rack

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 7.3 Eliminating Leakage in the Solvent Rack The leak sensor is installed inside the SRD and reports a leak when liquid collects in the drip tray under the fluid connections. Locate the source for the leak, eliminate the cause, and dry the leak sensor.
  • Page 52 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 46 Operating Instructions...

  • Page 53: Technical Information

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 8 Technical Information Bottle capacity: Eight 1-L reservoirs or four 2.5-L reservoirs or two 5-L reservoirs or five 1-L reservoirs and one 5-L reservoir or three 2,5-L reservoirs and two 1-L reservoirs or three 1-L reservoirs and two 2.5-L reservoirs or sixteen 0.5-L reservoirs...
  • Page 54 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 48 Operating Instructions...

  • Page 55: Accessories, Consumables, And Spare Parts

    The following accessories are shipped with the instrument (the list is subject to change without notice). The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Dionex sales organization.
  • Page 56 UltiMate 3000 Series: SRD-3x00 Solvent Racks Description Quantit Part No. in the SRD-3400 accessories, including: Cable connecting the SRD and the UltiMate 3000 system 6000.1006 module (pump, NCS-3500RS, or NCP-3200RS) Cap (to close the holes in the solvent bottle caps) Included in 6000.0047...

  • Page 57: Optional Accessories

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 9.2 Optional Accessories Description Part No. Remarks Drain kit for 6040.0005 The drain kit includes all required components and detailed UltiMate 3000 systems installation instructions. External power supply 6510.0004 To connect the SRD to the power source if the SRD is not...
  • Page 58 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 52 Operating Instructions...

  • Page 59: Pin Assignment

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 10 Pin Assignment 10.1 15-pin D-Sub Port Signal Name Signal Level Remarks Reserved Solvent Rack Error TTL_high with SRD errors Jumper to pin 9 Solvent Rack Leak TTL high with SRD leaks Reserved V_Degas...
  • Page 60 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 54 Operating Instructions...

  • Page 61: Index

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 11 Index 15-pin D Sub............19 Leak..............33 Accessory.............49, 51 Leak Monitoring..........33 Leak Sensor ..........13, 45 Chromeleon Diagnostics............33 Maintenance ............35 Error Messages ..........37 Maintenance Intervals ........35 Leak Monitoring ...........33 Messages ............38 Operation ............30...
  • Page 62 UltiMate 3000 Series: SRD-3x00 Solvent Racks Shut Down ............34 Site..............15 Unit Description (Overview)....... 9 Solvent Unpacking ............15 Compatibility ..........27 Degasser Compatibility.........27 Select.............27 Solvent Rack Leak..........33 Vacuum ............. 33 Solvent Reservoirs..........22 Vacuum Degasser (Solvent Compatibility)..27 Connect ............22 Vacuum Monitoring .......... 33 General Notes ..........22...

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