Dionex ICS-1000 Operator's Manual
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Dionex ICS-1000 Operator's Manual

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ICS-1000 ION CHROMATOGRAPHY SYSTEM
OPERATOR'S MANUAL
© 2003 Dionex Corporation
Document No. 031879
Revision 01
March 2003

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Summary of Contents for Dionex ICS-1000

  • Page 1 ICS-1000 ION CHROMATOGRAPHY SYSTEM OPERATOR’S MANUAL © 2003 Dionex Corporation Document No. 031879 Revision 01 March 2003...
  • Page 2 Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

  • Page 3: Table Of Contents

    Introduction to Ion Chromatography (IC) ..... . .1-1 Overview of the ICS-1000 ........1-4 About This Manual .
  • Page 4 ICS-1000 Ion Chromatography System 2.4.4 Injection Valve ........2-24 2.4.5...
  • Page 5 Contents 3.10 Loading and Injecting Samples ......3-14 3.10.1 Loading Samples with a Syringe ..... .3-15 3.10.2 Loading Samples with a Vacuum Syringe .
  • Page 6 ICS-1000 Ion Chromatography System 4.13 Baseline Noise or Drift ........4-19 4.14...
  • Page 7 Connecting the Power Cord ....... . B-11 Turning On the ICS-1000 Power ......B-12...
  • Page 8 Setting Up Chromeleon ........B-13 B.8.1 Assigning the ICS-1000 to a Timebase ....B-13 B.8.2 Assigning DX-LAN Devices to the Timebase (Optional) .
  • Page 9 Contents B.20.1 Connecting the Gas Source (Optional) ....B-47 B.20.2 Pressurizing the Eluent Reservoir (Optional) ... . B-48 B.21 Pump Continuous Seal Wash Connections (Optional) .
  • Page 10 ICS-1000 Ion Chromatography System E.10 How do I shut off the system? ....... E-2 E.11...

  • Page 11: Introduction

    1 • Introduction Introduction to Ion Chromatography (IC) The Dionex ICS-1000 Ion Chromatography System (ICS-1000) performs ion analyses using suppressed or non-suppressed conductivity detection. An ion chromatography system typically consists of a liquid eluent, a high-pressure pump, a sample injector, a guard and separator column, a chemical suppressor, a conductivity cell, and a data collection system.
  • Page 12 1. Eluent Delivery • Eluent, a liquid that helps to separate the sample ions, carries the sample through the ion chromatography system. The ICS-1000 is an isocratic delivery system. This means that the eluent composition and concentration remain constant throughout the run.
  • Page 13 • As the eluent and sample are pumped through the separator column, the sample ions are separated. In the ICS-1000, the mode of separation is called ion exchange. This is based on the premise that different sample ions migrate through the IC column at different rates, depending upon their interactions with the ion exchange sites.

  • Page 14: Overview Of The Ics-1000

    The ICS-1000 can optionally be configured with a column heater for temperature control of the column. ICS-1000 operation is controlled remotely by a personal computer running Windows 2000 or Windows XP and Chromeleon software (version 6.5 SP2 or...

  • Page 15: About This Manual

    1 • Introduction About This Manual Introduces ion analysis and the ICS-1000; explains the Chapter 1 Introduction conventions used in this manual, including safety-related information. Chapter 2 Provides an overview of ICS-1000 operating features and Features system components; introduces the Chromeleon user Overview interface.

  • Page 16: Safety Messages And Notes

    1.3.1 Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and/or damage to the ICS-1000 when properly followed. Safety messages appear in bold type and are accompanied by icons, as shown below. Indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.
  • Page 17 1 • Introduction Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr. Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen. Bedeutet eine mögliche Gefährdung. Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen. Bedeutet eine mögliche Gefährdung. Mißachtung kann zu kleineren oder mittelschweren Verletzungen führen. Wird auch verwendet, wenn eine Situation zu schweren Schäden am Gerät führen kann, jedoch keine Verletzungsgefahr besteht.

  • Page 18: Safety Labels

    1010.1-92 (safety), UL 3101-1/10.93 (safety), EN 50082-1:1992 (susceptibility), EN 55011:1991 (emissions). The TUV GS, C, US Mark safety labels and the CE Mark label on the ICS-1000 attest to compliance with these standards. The symbols below appear on the ICS-1000 or on ICS-1000 labels.

  • Page 19: Features

    2 • Features This chapter describes key ICS-1000 features and introduces the Chromeleon user interface. Operating Features 2.1.1 Front Panel Figure 2-1 illustrates the front panel of the ICS-1000. Injection Port The sample to be analyzed can be injected manually into the injection port, using a syringe.
  • Page 20 The suppressor is turned off Column heater is at set Column heater is transitioning to a new Column Heater temperature. temperature. Table 2-1. ICS-1000 Status LED States Doc. 031879-01 3/03...

  • Page 21: Top Cover

    2 • Features 2.1.2 Top Cover Figure 2-3 illustrates the top cover of the ICS-1000. Reservoir Storage The ICS-1000 top cover has room for up to three 2-L plastic reservoirs (P/N 044129). Tubing Chase The tubing chase under the top cover routes tubing to the front of the ICS-1000.

  • Page 22: Component Panel

    ICS-1000 Ion Chromatography System 2.1.3 Component Panel Figure 2-4 shows the user-accessible components installed on the component panel behind the ICS-1000 front door. Eluent Valve Leak Sensor Pump Heads Pressure Transducer Injection Valve Columns Connector for optional column heater Suppressor...
  • Page 23 Chromeleon and the Alarm LED flashes. Pump Heads The ICS-1000 includes a serial isocratic dual-head pump. The flow rate can be set to 0.00 and to between 0.05 and 5.0 mL/min. However, for optimum performance, set the flow rate to between 0.4 and 2.0 mL/min.
  • Page 24 Tubing Chases The upper tubing chase routes tubing from the top cover to the component panel. The lower tubing chase routes tubing from the component panel through the ICS-1000 interior to the rear panel. Doc. 031879-01 3/03...

  • Page 25: Rear Panel

    2 • Features 2.1.4 Rear Panel Figure 2-5 illustrates the ICS-1000 rear panel. Tubing Clips Analog Output Connector Power Switch Connections TTL and Relay Main Power Connector Strip Receptacle Waste Lines Figure 2-5. ICS-1000 Rear Panel Analog Output Connector The analog output connector outputs conductivity data (scaled to 1 volt) to an integrator or recording device.
  • Page 26 The tubing clips hold tubing in place when routing it from the top cover. Power Switch The power switch provides on/off control of power to the ICS-1000. Main Power Receptacle The power supply cord plugs into the AC power receptacle.

  • Page 27: Flow Schematic

    2 • Features Flow Schematic Figure 2-6 illustrates the liquid flow path through an ICS-1000 when using suppression in autorecycle mode. WASTE ELUENT PUMP DEGAS (Optional) COND CELL PULSE DAMPER PRESS. XDUCER VALVE PUMP PUMP HEAD HEAD WASTE ELUENT VALVE Figure 2-6.
  • Page 28 ICS-1000 Ion Chromatography System • Eluent from the eluent reservoir flows first through the pump degas assembly (if it is installed) and then through the eluent valve to the pump . It is then pushed through the pressure transducer , which measures the system pressure.

  • Page 29: The Chromeleon Interface

    NOTE This section provides a brief overview of the Chromeleon interface. For details, refer to the Chromeleon Help or user’s manual. Chromeleon software is used for remote control of ICS-1000 operation. Two modes of software control are available: direct control and automated control. •...

  • Page 30: The Chromeleon Main Window And Browser

    ICS-1000 Ion Chromatography System 2.3.1 The Chromeleon Main Window and Browser When you start Chromeleon, the main window and the Chromeleon Browser appear (see Figure 2-7). Figure 2-7. Chromeleon Main Window and Browser NOTE The Chromeleon Browser looks and functions like Windows Explorer, but do not confuse the two.

  • Page 31: The Ics-1000 Control Panels

    The Wellness Panel is described in Section 5.1. System Control Panels System Control Panels for the ICS-1000 are located in the Dionex Templates/Panels/Dionex_IC\ICS-1000 folder. Chromeleon provides panels for each ICS-1000 system configuration. There are two styles of panels: •...
  • Page 32 ICS-1000 Ion Chromatography System Figure 2-8. ICS-1000 System Control Panel (Traditional) Figure 2-9. ICS-1000 System Control Panel (Graphical) 2-14 Doc. 031879-01 3/03...
  • Page 33 2 • Features System Controls Overview This section provides brief descriptions of the various controls available on the ICS-1000 System Control Panels. NOTE The examples are from the traditional style of panel. Sample Datasource Displays the database in which the chromatographic data for the sample is saved.
  • Page 34 ICS-1000 Ion Chromatography System Audit Trail The Audit Trail displays an account of every event that occurs during ICS-1000 operation. This includes errors, status messages, operational events, etc. Pump Eluent Bottle The entry field and gauge indicate the level of liquid (in liters) in the eluent bottle. This is a user controlled feature.
  • Page 35 Set Temp Sets the temperature of the column heater. Detector Connected Connects the ICS-1000 to the Chromeleon Control Panel, enabling communication between the PC and the module. Clearing the Connected check box disconnects the ICS-1000 from the PC. Autozero Sets the conductivity to zero.
  • Page 36 Sets the current supplied to the suppressor. Cell Temp Sets the temperature of the cell. SRS Mode Selects whether the suppressor is on or off. Show Plot Displays the ICS-1000 detector signal plot in a separate window. 2-18 Doc. 031879-01 3/03...

  • Page 37: System Component Details

    • Tubing, fittings, and other accessories By default, the ICS-1000 monitors the degas pressure reading and turns the degas pump on and off as needed. A different degas operating mode can be selected from Chromeleon. To select degas operating options from...
  • Page 38 • : The degas pump is always on. This setting is for test Always On purposes by a Dionex Service Representative. Do not use this setting for routine operation. • : The degas pump cycles on and off according to the times...

  • Page 39: Eluent Valve

    Figure 2-11. Eluent Valve 2.4.3 Pump The ICS-1000 pump is a microprocessor-based isocratic eluent delivery system. Its variable speed, dual-piston series design ensures pulse-free pumping for the most demanding applications. Primary Pump Head The primary pump head pumps mobile phase into the secondary head (see Figure 2-12).
  • Page 40 Inlet Check Waste Valve Valve Figure 2-12. ICS-1000 Pump Components To open the priming valve, turn the knob one-quarter to one-half turn counterclockwise. When the priming valve is open, liquid can flow into and out of the primary pump head via the port in the front of the valve.
  • Page 41 For continued protection of the pump, replace the piston rinse seals (see Section 5.6) and O-rings in the seal wash assembly every 6 months, or whenever you replace the main piston seals for the ICS-1000 pump. 2-23 Doc. 031879-01 3/03...

  • Page 42: Injection Valve

    A 25-µL PEEK sample loop (P/N 042857) is installed between ports L (1) and L (4). Dionex offers 2-24 Doc. 031879-01 3/03...
  • Page 43 2 • Features sample loops in various sizes. If necessary, the pre-installed 25-µL loop can be replaced with a loop that has a different sample injection volume. Figure 2-14. Injection Valve Plumbing 2-25 Doc. 031879-01 3/03...

  • Page 44: Column Heater (Optional)

    ICS-1000 Ion Chromatography System 2.4.5 Column Heater (Optional) The column heater provides temperature control for the separator and guard column. The heater temperature can be set to between 30 °C and 60 °C and it must be set at least 5 °C above the ambient temperature.

  • Page 45: Suppressor

    For details about any of the suppressors or for information about selecting a suppressor for your application, refer to the suppressor manuals. The manuals are on the Dionex Reference Library CD-ROM (P/N 053891), located in the ICS-1000 Ship Kit (P/N 057905).
  • Page 46 ICS-1000 Ion Chromatography System DS6 Heated Conductivity Cell Components The cell front cover provides fittings for CELL IN CELL OUT connecting the cell to the suppressor (see Figure 2-4). The remaining cell components are mounted behind the component panel. To replace, the cell remove the screws on the cell front cover and pull the entire cell assembly out through the component panel.

  • Page 47: Operation And Maintenance

    This chapter describes routine operating and maintenance procedures for the ICS- 1000 Ion Chromatography System. Operation Overview Figure 3-1 illustrates the basic steps for routine operation of the ICS-1000. Pow er up the system Start Chromeleon Set up the reservoir...
  • Page 48 Figure 3-2 shows the typical steps for manual and batch sample processing. Samples can be loaded into the sample loop with an autosampler or with a syringe through the injection port on the ICS-1000 front door. Manual Sam ple Batch Sam ple...

  • Page 49: Power Up The System

    Suppressor Off* Cell heater Set to the last value used. The default when the ICS-1000 is turned on for the first time is 35 °C. Column oven temperature Set to the last value used. The default when the (Optional) ICS-1000 is turned on for the first time is 30 °C.

  • Page 50: Start Chromeleon

    Section B.8 for how to set up Chromeleon, if you are beginning operation of an ICS-1000 that has not been configured in a timebase. 1. If the Chromeleon Server is not running (the icon on the taskbar is crossed out...
  • Page 51 You can choose either style of panel, depending on your personal preference. 4. Open a panel for your system configuration by double-clicking its name in the list. For example, if your system includes an ICS-1000 and an AS50 autosampler, double-click either ICS-1000_Traditional_System_AS50.pan or ICS-1000_System_AS50.pan.

  • Page 52: Set Up The Eluent Reservoir

    Prepare the eluent according to the instructions in the column manual. Column manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). 3.4.2 Degas the Eluent Eluent quality significantly affects the performance of the ICS-1000. To ensure optimal pump performance, observe the following precautions: Doc. 031879-01 3/03...

  • Page 53: Filter The Eluent

    • The optional vacuum degas assembly provides programmable, online degassing. The degas assembly must be installed in the ICS-1000 at the factory. If the ICS-1000 does not contain a degas assembly, manually degas eluents daily (following the instructions below) and store it in pressurized reservoirs.

  • Page 54: Fill The Reservoir

    In order for the eluent level displayed on the Control Panel to be accurate, the level must be entered by the user when the reservoir is filled. The ICS-1000 does not automatically detect when the reservoir is filled, nor when it is empty.

  • Page 55: Check All Connections

    Select . The pump will begin pumping at approximately 3.0 mL/min. 4. Continue priming the ICS-1000 until all air and previous eluent are purged and no air bubbles are exiting the waste line. Doc. 031879-01 3/03...

  • Page 56: Set System Operating Conditions

    ICS-1000 Ion Chromatography System 5. Press Pump Off 6. Close the waste valve. Do not overtighten. The pump is now ready for operation. Set System Operating Conditions NOTE This section is an overview of the steps needed to start up the system and begin running samples. Actual...

  • Page 57: Equilibrate The System And Verify Operational Status

    3 • Operation and Maintenance Equilibrate the System and Verify Operational Status 1. Allow the system to equilibrate. During equilibration, the Chromeleon Control Panel displays the background conductivity (the conductivity of the eluent before sample injection) and the system backpressure. 2.

  • Page 58: Prepare Samples

    Filter groundwater and wastewater samples through 0.45 µm filters before injection, unless samples were filtered after collection. Before injection, pretreat samples that may contain high concentrations of interfering substances by putting them through Dionex OnGuard ® cartridges. Refer to the Installation and Troubleshooting Guide for OnGuard Cartridges (Document No.

  • Page 59: Diluting Samples

    3 • Operation and Maintenance 3.9.3 Diluting Samples Because the concentrations of ionic species in different samples can vary widely from sample to sample, no single dilution factor can be recommended for all samples of one type. In some cases (for example, many water samples), concentrations are so low that dilution is not necessary.

  • Page 60: Loading And Injecting Samples

    Section B.17 for AS50 autosampler installation instructions. After the AS50 is configured for use with the ICS-1000, all AS50 modes of injection (full-loop, partial-loop, and partial-loop limited-sample) are supported. In addition, all AS50 injection valve commands (whether issued from the AS50 front panel or from Chromeleon) will control the ICS-1000 injection valve.

  • Page 61: Loading Samples With A Syringe

    3-9). Figure 3-9. Injection Valve Connections 2. Fill the 1 cc syringe (P/N 016388) provided in the ICS-1000 Ship Kit (P/N 057905) with a calibration standard or sample. 3. Insert the syringe into the injection port on the ICS-1000 front door.

  • Page 62: Loading Samples With A Vacuum Syringe

    2. Place the free end of the line into the sample. 3. Verify that the injection valve is in the Load position. 4. Insert the 1 cc syringe (P/N 016388) provided in the ICS-1000 Ship Kit (P/N 057905) into the injection port on the ICS-1000 front door...

  • Page 63: Injecting Samples

    3 • Operation and Maintenance 3.10.4 Injecting Samples After loading the sample in the sample loop, switch the injection valve to the Inject position, using one of the following methods: • Manually: Click the button on the Chromeleon Control Panel Inject (see Figure...

  • Page 64: Process Samples

    Chromeleon menu bar, toolbars, and Control Panel. Commands are executed as soon as they are entered. 1. Complete the steps in Sections through to prepare for operation and start up the ICS-1000. 2. Load the sample using a syringe, vacuum syringe, or autosampler (see Section 3.10).

  • Page 65: Automatic (Batch) Sample Processing

    For each sample, the sequence includes a program with commands and parameters for controlling the ICS-1000, controlling the autosampler (if used), and acquiring sample data. The sequence also includes a quantification method for peak identification and area determination. Additional sample processing parameters are included in the sequence (for example, the sample name, sample type, injection volume, etc.).
  • Page 66 ICS-1000 Ion Chromatography System 3. To start the sample processing, make sure the System Control Panel is open (see Section 3.3) and is the active window. Then, select Edit from the Batch menu. The Batch dialog box opens (see Figure 3-10).

  • Page 67: Maintenance

    3 • Operation and Maintenance 3.12 Maintenance This section describes routine maintenance procedures that users may perform. All other maintenance procedures must be performed by Dionex personnel. As Needed • Regularly check the eluent reservoir to see if it needs to be refilled.
  • Page 68 ICS-1000 Ion Chromatography System Every Six Months • Calibrate the cell (see Section 5.1.2). • Calibrate the vacuum degas assembly (see Section 5.1.4). • Replace the pump piston rinse seals and piston seals (see Section 5.6). Yearly • Rebuild the injection valve (see Section 5.4).

  • Page 69: Troubleshooting

    If you are unable to eliminate a problem, contact Dionex for help. In the U.S., call Dionex Technical Support at 1-800-346-6390. Outside the U.S., call the nearest Dionex office...
  • Page 70 ICS-1000 Ion Chromatography System Alarms and Error Conditions SUPPRESSOR STOPPED FOR FLOW RATE page 4-9 Table 4-3. ICS-1000 Alarms and Error Messages Summary (Continued) Other Operating Problems Liquid Leaks page 4-9 Pump Difficult to Prime or Loses Prime page 4-11...

  • Page 71: Alarms And Error Conditions

    Chromeleon Audit Trail. COLUMN HEATER EXCEEDS SAFE TEMPERATURE This error occurs when the column heater temperature is higher than the maximum allowed. This error may occur if the ICS-1000 is operating in an extreme environment (greater than 40 °C) To troubleshoot: •...

  • Page 72: Degas Calibration Failed

    2. If calibration still fails, the vacuum degas assembly may need to be replaced. Contact Dionex for assistance. HARDWARE NOT PRESENT This error indicates an error in the ICS-1000 electronics. To troubleshoot: • Contact Dionex for assistance. The ICS-1000 electronics components cannot be serviced by the user.

  • Page 73: Option Not Installed

    4 • Troubleshooting LOAD/INJECT VALVE ERROR If the injection valve fails to switch position within 1 second of being toggled, the ICS-1000 Moduleware reports an error to Chromeleon and the “Load/Inject valve error” message appears. To troubleshoot: 1. If a sequence is being executed, terminate the sequence by selecting Stop on the Chromeleon Batch menu.

  • Page 74: Pump Motor Lost Control

    PUMP OVER PRESSURE The ICS-1000 has a maximum system pressure limit of 34.5 MPa (5000 psi). The pressure limit can be lowered from Chromeleon (in the Server Configuration or in the control program). If the system pressure exceeds the set limit for at least 0.5 seconds, the pump stops and the “Pump over pressure”...

  • Page 75: Pump Under Pressure

    4 • Troubleshooting PUMP UNDER PRESSURE The ICS-1000 has a minimum pressure limit of 0. The low pressure limit can be raised from Chromeleon (in the Server Configuration or in the control program). If the system pressure falls below the low pressure limit, the pump stops and the “Pump under pressure”...

  • Page 76: Suppressor Over Voltage

    ICS-1000 Ion Chromatography System SUPPRESSOR OVER VOLTAGE This error appears if you turn on the suppressor and the ICS-1000 cannot establish a connection with the suppressor. To troubleshoot: 1. Check the suppressor cable connection (see Section 5.10). 2. If the error persists, replace the suppressor (see Section 5.10).

  • Page 77: Liquid Leaks

    Make sure the lines are not crimped or otherwise blocked. Also, if the blocked line is a waste line, make sure it is not elevated at any point after it exits the ICS-1000. If a line is blocked, replace it (see Section 5.3).
  • Page 78 Leaking pressure transducer Make sure the liquid line connections into the pressure transducer are tight. Refer to Installation of Dionex Liquid Line Fittings (Document No. 031432) for tightening requirements. The manual is on the Dionex Reference Library CD-ROM (P/N 053891). Replace any damaged fittings. The pressure transducer cannot be serviced by the user.

  • Page 79: Pump Difficult To Prime Or Loses Prime

    Empty eluent reservoir and/or no eluent connected Fill the reservoir. Make sure all connections are secure. • Eluent improperly or insufficiently degassed If the ICS-1000 does not contain a vacuum degas assembly, degas the eluent manually (see Section 3.4.2). If the ICS-1000 contains a vacuum degas assembly, check the degas settings: 1.
  • Page 80 ICS-1000 Ion Chromatography System Figure 4-1. ICS-1000 Server Configuration Properties: Options 3. Verify that the Degas check box is enabled under Installed Options. 4. Check the Degas Mode settings. Select either the Monitor option or the Cycle option. If you select Cycle, set the time on to 30 seconds and the time off to 10 minutes.

  • Page 81: Pump Does Not Start

    • Liquid leaks at junction between pump head and pump casting Use the open-end wrench (P/N 014605) provided in the ICS-1000 Ship Kit (P/N 057905) to tighten the two acorn nuts that attach the pump head to the pump housing (see Figure 5-4).

  • Page 82: No Flow

    ICS-1000 Ion Chromatography System No Flow • Pump waste or priming valve open (see Figure 4-2). Close the valves by turning the knobs clockwise until fingertight. DO NOT OVERTIGHTEN! Overtightening may damage the valve and the pump head. Priming Valve Waste Valve Figure 4-2.

  • Page 83: Erratic Flow/Pressure Reading

    Restriction in the system plumbing Check all liquid lines for crimping or blockage. Make sure the ferrule fittings are not overtightened onto tubing. Refer to Installation of Dionex Liquid Line Fittings (Document No. 031432) for details. The manual is on the Dionex Reference Library CD-ROM (P/N 053891).

  • Page 84: Peak "Ghosting

    Clogged column bed supports Refer to the instructions in the column manual for troubleshooting guidance. Column manuals are included on the Dionex Reference Library CD-ROM (P/N 053891), located in the ICS-1000 Ship Kit (P/N 057905). • Contaminated columns Clean the columns as instructed in the column manual.

  • Page 85: Nonreproducible Peak Height Or Retention Time

    4 • Troubleshooting • Malfunctioning injection valve 1. Rebuild the injection valve (see Section 5.4). 2. If the valve leak persists, contact Dionex for assistance. Nonreproducible Peak Height or Retention Time • Column overloading Dilute the sample (see Section 3.9).

  • Page 86: No Cell Response

    Verify that the suppressor is turned on and the current is set to the correct value. Refer to the suppressor manual for additional troubleshooting guidance. Suppressor manuals are included on the Dionex Reference Library CD-ROM (P/N 053891), located in the ICS-1000 Ship Kit (P/N 057905). • Sample concentration too high...

  • Page 87: Baseline Noise Or Drift

    Flow system leak; erratic baseline Check all fittings and liquid lines for leaks. Tighten or, if necessary, replace all liquid line connections. Refer to Installation of Dionex Liquid Line Fittings (Document No. 031432) for tightening requirements. The manual is on the Dionex Reference Library CD-ROM (P/N 053891).

  • Page 88: Vacuum Degas Assembly Does Not Run

    Degas option not enabled in Chromeleon a. Open the Chromeleon Server Configuration program. Select the ICS- 1000 device icon under the timebase and select Properties from the Edit menu (or double-click the ICS-1000 device icon). Select the Options tab (see Figure 4-3).
  • Page 89 Always On option. The pump should turn on immediately. If it does not, the vacuum degas assembly may need to be replaced. Contact Dionex for assistance. Select the Degas Mode, Always On option only when testing the vacuum degas assembly.
  • Page 90 ICS-1000 Ion Chromatography System 4-22 Doc. 031879-01 3/03...

  • Page 91: Service

    5 • Service This chapter describes ICS-1000 service and repair procedures that the user can perform. All procedures not included here, including electronics-related repair procedures, must be performed by Dionex personnel. For assistance, contact Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S., call the nearest Dionex office.

  • Page 92: Chromeleon Wellness Panel Overview

    3. In the right pane of the Browser window, double-click ICS- 1000_1500_2000_Wellness_user.pan.The Wellness Panel appears. 4. Open the Control menu and click the name of the ICS-1000 timebase at the bottom of the menu. This connects the Wellness Panel to the ICS-1000 and enables the controls on the panel.
  • Page 93 Calibrate the pump flow rate • Calibrate the vacuum degas assembly • Calibrate the conductivity cell • Test the conductivity cell electronics, using a dummy cell • Upload calibration values from the ICS-1000 to the Chromeleon Wellness database Doc. 031879-01 3/03...

  • Page 94: Calibrating The Conductivity Cell

    Backpressure tubing to provide Use 0.076-mm (0.003-in) ID yellow PEEK at least 7 MPa (1000 psi) tubing (P/N 049715). 1. Open the ICS-1000 Wellness Panel in Chromeleon (see Section 5.1.1). 2. On the Wellness Panel under Electric Conductivity Cell Calibration, click Offset Cal.
  • Page 95 After calibration, the conductivity reading should be 147.00 ± 2 and the cell constant should be between 120 and 180. If this is not the case, contact Dionex for help. 10. Click Log to record the new calibration values in the Audit Trail.

  • Page 96: Calibrating The Flow Rate

    ICS-1000 Ion Chromatography System 5.1.3 Calibrating the Flow Rate When to Calibrate If you run the ICS-1000 Operational Qualification or Performance Qualification and it fails. Items Needed • 0.076 mm (0.003 in) ID yellow PEEK tubing (P/N 049715) to create 14 ±...

  • Page 97: Calibrating The Vacuum Degas Assembly

    Calibrating the Vacuum Degas Assembly When to Calibrate • After every 6 months of use 1. Open the ICS-1000 Wellness Panel in Chromeleon (see Section 5.1.1). 2. On the Wellness Panel under Degas Calibration, select Calibrate. The degas pump runs for 90 seconds to allow a vacuum to be created.
  • Page 98 COND CELL PULSE DAMPER PRESS. XDUCER VALVE PUMP PUMP HEAD HEAD WASTE ELUENT VALVE Figure 5-2. ICS-1000 Flow Schematic NOTE The numbers on the flow schematic indicate the order in which liquid flows through the system components. Doc. 031879-01 3/03...

  • Page 99: Replacing Tubing And Fittings

    5 • Service Replacing Tubing and Fittings The ICS-1000 is plumbed with the tubing and tubing assemblies listed below. Tubing Size and Type Color Used For 0.125-mm (0.005-in) ID PEEK Connection from pump pulse damper (P/N 044221) to pressure transducer 0.25-mm (0.010-in) ID PEEK...

  • Page 100: Rebuilding The Injection Valve

    NOTE Substitution of non-Dionex parts may impair valve performance and void the product warranty. 1. Turn off the pump from the ICS-1000 Control Panel in Chromeleon. 2. Open the ICS-1000 front door. 3. Disconnect each liquid line connected to the injection valve.

  • Page 101: Cleaning And Replacing The Pump Check Valves

    If new check valves are not available, follow the instructions for cleaning. Replacing Check Valves 1. Close the eluent valve from the ICS-1000 Control Panel in Chromeleon. 2. Turn off the main power switch, to ensure that you do not unintentionally start the ICS-1000.
  • Page 102 10. Prime the pump (see Section B.12). 11. When the ICS-1000 is at operating pressure, check for leaks from the check valves. Tighten a check valve a little more only if it leaks. Cleaning Check Valves 1. Carefully remove the check valve cartridges from the valve housings.

  • Page 103: Replacing A Pump Piston Seal And Piston Rinse Seal

    1. Turn off the main power switch, to ensure that you do not unintentionally start the ICS-1000. 2. Disconnect all tubing connections to the pump head. 3. Locate the open end wrench (P/N 014605) provided in the ICS-1000 Ship Kit (P/N 057905). Use the wrench to remove the two acorn nuts (see Figure 5-4) from the pump head.
  • Page 104 ICS-1000 Ion Chromatography System 5. Place the head (front end down) on a clean work surface and lift off the spacer to expose the piston seal (see Figure 5-5 Figure 5-6). Priming Valve Knob (P/N 055709) Outlet Check Valve Assembly...
  • Page 105 5 • Service 6. The piston does not come off as part of the pump head assembly because it is captured by a magnetic retention system. After removing the pump head, hold the shaft of the piston (near the base), tilt the piston slightly, and pull the piston away from the pump.
  • Page 106 ICS-1000 Ion Chromatography System 7. In one hand, hold the guide with the O-ring and piston rinse seal facing up (this prevents the O-ring from falling out). In the other hand, hold the spacer with the cavity facing down. 8. Gently press the guide into the cavity in the spacer until it is fully seated.

  • Page 107: Replacing A Pump Piston

    5 • Service Reinstalling the Head and Piston Dionex recommends reinstalling the head and piston as a single assembly, so that the piston centers itself onto the magnetic follower. 1. Hold the assembled spacer and guide with the drain tubes aligned vertically and press the spacer into the head until it is flush with the indented surface of the head.

  • Page 108: Replacing The Waste Valve Or Priming Valve O-Ring

    ICS-1000 Ion Chromatography System Replacing the Waste Valve or Priming Valve O-Ring A damaged O-ring causes leakage around the base of the waste valve or priming valve knob. 1. Close the eluent valve from the Control Panel in Chromeleon. 2. Turn off the main power switch, to ensure that you do not unintentionally start the ICS-1000.
  • Page 109 5 • Service Valve Knob O-Ring (P/N 055752) Figure 5-8. Waste Valve or Priming Valve O-Ring Replacement 5. Slide a new O-ring (P/N 055752) over the end of the valve. 6. To reinstall the valve containing the new O-ring, turn the knob clockwise and then tighten fingertight.

  • Page 110: Replacing The Conductivity Cell

    ICS-1000 Ion Chromatography System Replacing the Conductivity Cell 1. Turn off the ICS-1000 power. 2. Open the front door and disconnect the tubing from the CELL IN CELL fittings (see Figure 5-9, View A). Loosen the two screws on the cell front cover.
  • Page 111 S, is automatically enabled. The slope value is applied to raw conductivity readings. • Enter the cell constant measured at the factory. The ICS-1000 uses the cell constant to determine the measured conductivity. a. Open the Wellness Panel (see Section 5.1.1)

  • Page 112: Replacing The Suppressor

    Figure 5-11. Removing the Suppressor 8. Connect the cable from the new suppressor to the ICS-1000 cable (align the pins on the two connectors and push them together). 9. Orient the suppressor with the...

  • Page 113: Replacing The Column Heater

    5.11 Replacing the Column Heater 1. Turn off the ICS-1000 power. 2. Open the front door of the ICS-1000. 3. Follow the steps below to remove the existing column heater: a. Unscrew the two thumbscrews on the heater cover (they remain attached to the cover).
  • Page 114 Figure 5-13) and remove the plate. Thumbscrews (6) Figure 5-13. ICS-1000 Column Heater Cover Removed d. Remove the columns from the column heater. e. Pull the column heater straight toward you to remove it from the component panel. 4. Before installing the new column heater, write down the two calibration values (offset and slope) recorded on the back of the heater.

  • Page 115: Replacing The Column Heater Heat Exchanger

    Download to download the new calibration values to the ICS-1000. d. To ensure the new calibration values are recorded in the ICS-1000 memory, do not turn off the ICS-1000 power for at least 1 minute after downloading the values. 5.12 Replacing the Column Heater Heat Exchanger 1.

  • Page 116: Replacing The Eluent Valve

    11. Reinstall the top metal plate and the heater cover. 5.13 Replacing the Eluent Valve 1. Turn off the power to the ICS-1000. 2. Open the ICS-1000 front door. 3. To prevent an eluent leak during the procedure, disconnect the eluent line from the reservoir cap.
  • Page 117 Step Figure 5-17. Removing the Valve Mounting Plate 11. Connect the cable from the new valve to the ICS-1000 cable. Feed the cables back inside the ICS-1000. 12. Align the new valve on the component panel with the liquid ports facing up...

  • Page 118: Replacing The Leak Sensor

    Figure 5-19. Leak Sensor Cable 7. Connect the cable from the new leak sensor to the ICS-1000 cable. 8. Feed the cables back inside the ICS-1000. Align the leak sensor with the component panel opening and fingertighten the screw. 9. Make sure the leak sensor does not touch the bottom of the drip tray.

  • Page 119: Priming With Isopropyl Alcohol

    5 • Service 5.15 Priming with Isopropyl Alcohol NOTE Prime the pump heads with isopropyl alcohol only if the standard priming procedures described Section B.12 are unsuccessful. 1. Connect a 10-mL syringe (P/N 054578) filled with isopropyl alcohol (IPA) to the port in the primary pump head (see Figure B-37).

  • Page 120: Changing Main Power Fuses

    Figure 5-20) on the ICS-1000 rear panel. To remove the fuse holder, squeeze the tab on the top of the holder to release it and pull the holder straight out of its compartment. 3. Replace the two fuses in the holder with new IEC 127 fast-blow fuses rated 3.15 amps (P/N 954745).

  • Page 121: Electrical

    A • Specifications A.1 Electrical Main Power 85 to 265 Vac, 47 to 63 Hz. The ICS-1000 power supply is auto- sensing and requires no voltage adjustment Fuses Two 3.15 amp fast-blow IEC 127 fuses (P/N 954745) A.2 Physical Dimensions Height: 56.1 cm (22.1 in)

  • Page 122: Front Panel

    ICS-1000 Ion Chromatography System A.4 Front Panel Power LED On when power is present; off when no power is present Inject Valve Indicate the position of the inject valve. LEDs Alarm LED Indicates an alarm state. Status LEDs Indicate instrument status.

  • Page 123: Detector

    A • Specifications A.6 Detector 0 to 3200 µS (digital output) Range 0 to 3000 µS (analog output) Temperature 1.7% per °C Compensation Cell Drive 8 kH square wave Linearity Correlation >0.999 %RSD <5% to 800 µS A.7 Conductivity Cell with Heat Exchanger Cell Body PEEK Electrodes...

  • Page 124: Column Heater (Optional

    ICS-1000 Ion Chromatography System A.10 Column Heater (Optional) Operating 30 to 60 °C (86 to 140 °F); must be at least 5 °C above the ambient Temperature temperature Doc. 031879-01 3/03...

  • Page 125: B • Installation

    • Allow at least 15 cm (6 in) behind the ICS-1000 for power connections and ventilation. For optimal performance, install the ICS-1000 in a draft-free location, out of the path of air conditioning and heating vents.

  • Page 126: Unpacking The Ics-1000 System

    ICS-1000 Ion Chromatography System B.2 Unpacking the ICS-1000 System 1. After removing the ICS-1000 from the shipping box and removing the top layer of foam, remove the next layer of foam (see Figure B-1). Remove this foam Figure B-1. ICS-1000 After Removing Shipping Box and First Layer of Foam 2.
  • Page 127 (see Figure B-3). 5. Lift the ICS-1000 out of the bottom layer of foam and set it on a workbench. Inspect the ICS-1000 for any shipping damage. Two or more persons should lift the ICS-1000, which weighs 30 kg (66 lb).

  • Page 128: Unpacking The Computer (North America Only

    1. Refer to Installing the Chromeleon IC System (Document No. 031883) to verify that the computer being used meets Chromeleon specifications. The manual is on the Dionex Reference Library CD- ROM (P/N 053891). 2. Remove the central processing unit (CPU), keyboard, mouse, and all documentation from the computer box and place these items on a workbench.

  • Page 129: Installing Chromeleon

    B.3 Installing Chromeleon NOTES • Windows 2000 or Windows XP is required for controlling the ICS-1000 ® with Chromeleon. Chromeleon version 6.5 SP2 or later is required. • To install Chromeleon, you must log onto Windows 2000 or XP with administrator privileges.

  • Page 130: Installing The Chromeleon Software License

    (Document No. 031883). B.4 Installing the Chromeleon Software License For the Chromeleon computer to control the ICS-1000, a valid software license must be installed. To install the license, you first install a dongle on the computer and then enter a license “Key Code” in Chromeleon. (A dongle is an adapter that is connected to the parallel PC interface or USB port.
  • Page 131 Installing the Chromeleon Software License 3. Check the server status: • If “Chromeleon Server is running idle” is displayed, the license Key Code was entered before shipping the computer, and you can omit the remaining steps in this section. Click Close and go on to Section B.5.
  • Page 132 ICS-1000 Ion Chromatography System 5. Select File>Preferences to open the Preferences dialog box (see Figure B-5). Select the License tab and click Dongle. Enter the Key Code exactly as it appears on the Chromeleon Software License key code sheet. Click OK.

  • Page 133: Connecting The Ics-1000 To The Chromeleon Pc

    USB port on the Chromeleon PC. The rear panel also provides two USB ports for connecting other Dionex USB-compliant modules to the system. Do not turn on the power to the ICS-1000 or other USB module(s) until after you connect the USB cable.

  • Page 134: Connecting Additional Usb Devices

    (see Figure B-8). Figure B-8. Example Connections: Two Dionex USB Modules Connected to the ICS-1000 NOTE If the number of USB devices in the system exceeds the number of available USB ports, connect the devices to the system with a USB-compliant, full-speed external hub.

  • Page 135: Connecting The Power Cord

    B.6 Connecting the Power Cord Connect the power cord (IEC 320 C13) (ordered separately) from the main power receptacle on the rear panel to a grounded power source. The ICS-1000 power supply is auto-sensing, so no adjustment is required to select the line voltage.

  • Page 136: Turning On The Ics-1000 Power

    ICS-1000 Ion Chromatography System B.7 Turning On the ICS-1000 Power Before turning on the ICS-1000 power, verify that Chromeleon software is installed. See Section B.3 for installation instructions. If Chromeleon is not installed first, Windows will be unable to identify the new USB device.

  • Page 137: Setting Up Chromeleon

    Chromeleon. For details about any of these steps, refer to the Chromeleon user’s guide or online Help. B.8.1 Assigning the ICS-1000 to a Timebase 1. If the Chromeleon Server is not running (the icon on the taskbar is crossed out in red ), start the Server by right-clicking the icon and selecting Start Server.
  • Page 138 You can use the provided name or type a new one (for example, ICS-1000). 6. Press the Enter key. 7. In the Unassigned USB Devices box, select the ICS-1000 and then click the Assign button. The ICS-1000 is now assigned to the timebase (see Figure B-11).
  • Page 139 B-12). Figure B-12. Server Configuration with ICS-1000 Timebase 11. To verify that the ICS-1000 is correctly connected to the timebase, double-click its name under the timebase. Click Yes if the following message appears: “Communication to the device will be stopped…Do you want to continue?”...

  • Page 140: Assigning Dx-Lan Devices To The Timebase (Optional

    ICS-1000 Ion Chromatography System 12. The ICS-1000 System dialog box opens (see Figure B-13). The General page shows the Device Name (Pump_ECD), the Mode (Live), and the Module Serial No. Figure B-13. ICS-1000 System Properties 13. Close the dialog box and then save the configuration (select File>Save Installation).
  • Page 141 Setting Up Chromeleon 2. Select the timebase that was created for the ICS-1000 (see Figure B-14); then, click the Add device icon on the toolbar (or select Edit>Add Device). Figure B-14. Example: ICS-1000 Timebase The Add device to timebase dialog box appears.
  • Page 142 ICS-1000 Ion Chromatography System 4. Click OK. A configuration properties dialog box for the device appears. Figure B-16 shows an example of the properties for an AS50 Autosampler. Figure B-16. AS50 Configuration Properties 5. On the General tab page, clear the Demo Mode check box if it is currently selected, and then select the DX-LAN Device ID number.

  • Page 143: Installing And Plumbing The Columns And Suppressor

    The manuals are provided on the Dionex Reference Library CD-ROM (P/N 053891), which is located in the ICS-1000 Ship Kit (P/N 057905). Before beginning: • Remove the guard column, separator column, and suppressor from their boxes.

  • Page 144: Installing The Columns

    Figure B-18) and remove the plate. Thumbscrew (6) 5. Go on to Section B.9.2. Figure B-18. ICS-1000 Column Heater Cover Removed B.9.2 Installing the Columns 1. Remove the union connecting GUARD IN GUARD OUT 2. Connect to the guard column inlet. Check the arrow on the GUARD IN column label;...

  • Page 145: Installing The Suppressor

    Installing and Plumbing the Columns and Suppressor B.9.3 Installing the Suppressor 1. Locate the suppressor mounting tabs on the component panel (see Suppressor Figure B-19). Reach Mounting Tabs into the opening between the tabs and Suppressor Cable pull the yellow Connector connector and attached Guideline for AES...
  • Page 146 ICS-1000 Ion Chromatography System Press the suppressor onto the tabs and then slide it down to lock it in place. Pull slightly on the center of the suppressor to verify that it is securely fastened. Figure B-20 shows an SRS suppressor installed on the component panel.

  • Page 147: Connecting The Waste Lines

    WASTE TO WASTE OUT Direct the line from the top of the ICS-1000 to the rear WASTE, GAS SEPARATOR panel. Snap the line onto one of the tubing clips on the rear panel. Connect the line to the gas separator waste tube (see Section B.10.1).
  • Page 148 (see Figure B-22). NE FERMEZ PAS LE CONTENEUR DE GAZ RÉSIDUEL L : Le ICS-1000 travaille avec un proces d´électrolyse, qui produit des petites quantités de gaz d´oxygène ou d´hydrogène. Afin de garantir que le gaz ne soit pas enfermé dans un conteneur fermé et puisse s´y concentrer, connectez un tube noir à...

  • Page 149: Setting Up The Eluent Reservoir

    1. Rinse the eluent reservoir. 2. Prepare the eluent as instructed in the manual for the column being installed. The column manual is included on the Dionex Reference Library CD-ROM (P/N 053891), located in the Ship Kit. 3. Fill the reservoir with the prepared eluent.

  • Page 150: Setting Up A Chromeleon Application

    This section shows how to create a list of samples to be processed (a sequence) and how to use an application template. 1. Identify the type of column and suppressor installed in the ICS-1000. 2. Start Chromeleon (select Start>Programs>Chromeleon>Chromeleon).
  • Page 151 Setting Up a Chromeleon Application 3. In the Browser, select File>New and select Sequence (using Wizard) (see Figure B-24). Figure B-24. Creating a New Sequence The Sequence Wizard Welcome page appears (see Figure B-25). Figure B-25. Sequence Wizard Welcome Page B-27 Doc.
  • Page 152 NOTE For details about a Sequence Wizard step, click the Help button on the Sequence Wizard page. 4. Click Next>. The Sequence Wizard Timebase page appears (see Figure B-26). Figure B-26. Example: Sequence Wizard Step 1 5. Select the ICS-1000 timebase and click Next>. B-28 Doc. 031879-01 3/03...
  • Page 153 Setting Up a Chromeleon Application The Unknown Samples page appears (see Figure B-27). Figure B-27. Example: Sequence Wizard Step 2 6. Select Use Template from the drop-down list. 7. In the Template for Sample Name field, type Sample, click the right arrow, and select Sample Number #n.
  • Page 154 ICS-1000 Ion Chromatography System 10. Click Apply. Figure B-28 shows Sequence Wizard Step 2 after a sample is defined. Figure B-28. Example: Sequence Wizard Step 2 with Sample Defined 11. Click Next>. B-30 Doc. 031879-01 3/03...
  • Page 155 Setting Up a Chromeleon Application The Standard Samples page appears (see Figure B-29). Figure B-29. Example Sequence Wizard Step 3 12. Click Next> to omit this step and define standard injections later. Or, define one or more standard injections, and then click Next>. The Methods &...
  • Page 156 B-30, the 4-mm ASRS suppressor and AS17 column are selected. Figure B-30. Example: Sequence Wizard Step 4 15. To select a report template, click the Browse button, go to the Dionex Templates/Reports folder, and select a template (for example, default_letter). 16. Click Next>.
  • Page 157 Setting Up a Chromeleon Application 17. Enter a name for the sequence and a location (see the example in Figure B-31). Figure B-31. Example: Sequence Wizard Step 5 18. Click Next> and then click Done on the last page of the Sequence Wizard. The Browser appears, displaying the sequence you created (see Figure B-32).
  • Page 158 The .qnt file (quantification method) contains peak identification and area determination parameters. • The .pan file (Control Panel) lets you control and/or monitor the ICS-1000 and other modules that are connected to the timebase. NOTE The sequence that you create here is intended as a starting point only.

  • Page 159: Verifying Chromeleon Communication

    NOTE If the Connected check box is not selected, click the box to connect the ICS-1000 to the Control Panel. B.12.1 Verifying Chromeleon Communication Verify that Chromeleon is communicating with the ICS-1000 by clicking and then the button on the Control Panel. If...

  • Page 160: Priming The Pump

    ICS-1000 Ion Chromatography System B.14 Priming the Pump 1. Verify that the eluent reservoir is filled, the reservoir cap is installed and hand tightened, and the liquid line from the ICS-1000 to the reservoir cap is connected (see Section B.11).
  • Page 161 Priming the Pump 3. Open the priming valve by turning it one-quarter to one-half turn counterclockwise. 4. On the Chromeleon Control Panel, click and then Pump Settings click (see Figure B-36). Eluent Flow Valve Open Figure B-36. Chromeleon Control Panel: Pump Settings 5.

  • Page 162: Priming With The Prime Button

    Figure B-36). The Prime pump will begin pumping at approximately 3.0 mL/min. 4. Continue priming the ICS-1000 until no air bubbles are exiting the pump waste line. 5. Press Pump Off 6. Close the waste valve. Do not overtighten. The pump is now ready for operation.

  • Page 163: Equilibrating The System

    After the system has equilibrated, verify the actual pump pressure and stability by monitoring the pump pressure reading on the Chromeleon Control Panel. Write down the short-term pressure fluctuations; they should be less than 0.13 MPa (20 psi). ICS-1000 installation is complete. For routine operation, refer to Chapter B-39 Doc. 031879-01 3/03...

  • Page 164: Connecting An As50 Autosampler (Optional

    ICS-1000 Ion Chromatography System B.17 Connecting an AS50 Autosampler (Optional) B.17.1 AS50 Configuration Requirements To use an AS50 autosampler with the ICS-1000, the AS50 must meet the following requirements: • An injection valve cannot be installed in the AS50 •...

  • Page 165: As50 Connections

    B.17.2 AS50 Connections 1. Place the AS50 to the left of the ICS-1000 on the workbench. 2. Open the ICS-1000 and AS50 doors. 3. The needle seal line that extends from the inject port in the autosampler is coiled inside the AS50 door, on the right side.
  • Page 166 Teflon waste line (see Figure B-40) and routed through the tubing chase to the ICS-1000 rear panel. Figure B-39. ICS-1000 Injection Valve 5. Disconnect the green waste line (labeled AS50 ) from the union and TRAY...

  • Page 167: Enable As50 Control Of The Injection Valve

    B.17.3 Enable AS50 Control of the Injection Valve Follow the steps below if you are connecting an AS50 that was not configured at the factory for ICS-1000 support. Injection valve control is already enabled in pre-configured AS50 models (see the list in Section B.17.1).

  • Page 168: Connecting An As40 Automated Sampler (Optional

    ICS-1000 Ion Chromatography System B.18 Connecting an AS40 Automated Sampler (Optional) 1. Place the AS40 to the left AS40 of the ICS-1000 on the Sample workbench. Outlet Line 2. Open the front door of the ICS-1000 and thread the outlet line from the AS40...
  • Page 169 Chromeleon. When you set up the ICS-1000 application (see Section B.12), commands for controlling the AS40 Load relay and switching the ICS-1000 injection valve are included by default in the program file (see Figure B-45). B-45...

  • Page 170: Analog Output Connection (Optional

    Figure B-45. Example Program for AS40 Control B.19 Analog Output Connection (Optional) The analog output connector on the ICS-1000 rear panel outputs an analog voltage signal proportional to the conductivity measured by the cell. The output is filtered using the selected data rise time. The analog output can be connected to an analog-to-digital converter such as an integrator or other recording device.

  • Page 171: Connecting The Gas Source (Optional

    Pressurizing the Eluent Reservoir (Optional) B.20.1 Connecting the Gas Source (Optional) 1. Locate the barbed fitting (P/N 030071), pipe thread reducer (P/N 030087), 3-mm (1/8-in) ID tubing (P/N 040793), and ICS-1000 air regulator accessory (P/N 060054). These parts must be ordered separately.
  • Page 172 ICS-1000 Ion Chromatography System B.20.2 Pressurizing the Eluent Reservoir (Optional) NOTE Do not pressurize the ICS-1000 until after the reservoir is plumbed. 1. Pull out the air regulator knob and turn it fully counterclockwise to ensure there is no pressure to the reservoir when the gas source is turned on.

  • Page 173: Pump Continuous Seal Wash Connections (Optional

    10% or 20% isopropyl alcohol. (Isopropyl alcohol will inhibit bacterial growth in the water.) 2. Place the flush bottle on top of the ICS-1000. During operation, gravity maintains flow through the pump heads. 3. The flush solution is carried to the piston seal wash assembly and out to waste via 1.5-mm (0.06-in) ID tubing (P/N 055847).
  • Page 174 This is the waste line. Route this waste line through the lower tubing chase and out the back of the ICS-1000. c. Push the 15-cm (6-in) piece of clear tubing over the drain tube on the top the secondary pump head.

  • Page 175: Manually Connecting To A Control Panel

    Manually Connecting to a Control Panel B.22 Manually Connecting to a Control Panel This section describes how to manually connect the ICS-1000 to a Control Panel if you do not use the application templates described in Section B.12. 1. In the Browser, expand the Dionex Templates\Panels\Dionex_IC\ICS-1000 folder.
  • Page 176 ICS-1000 Ion Chromatography System 3. To connect the Control Panel to the ICS-1000, open the Control menu and click the name of the ICS-1000 timebase at the bottom of the menu. The Control Panel is now connected to the ICS-1000 and the controls are...

  • Page 177: Installation Troubleshooting

    Installation Troubleshooting B.23 Installation Troubleshooting Problem: When the ICS-1000 power is turned on for the first time, a Windows message box appears asking for a USB configuration file (cmwdmusb.inf). Possible Cause: The USB cable was connected and the power turned on before Chromeleon was installed.
  • Page 178 ICS-1000 Ion Chromatography System B-54 Doc. 031879-01 3/03...

  • Page 179: C • Ttl And Relay Control

    If there is a question regarding appropriate usage, contact Dionex. C.1 TTL and Relay Connections A 12-pin connector strip for TTL/relay control is located on the ICS-1000 rear panel. The connector provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure C-1).
  • Page 180 When attaching wires to the connector plug, be careful not to allow stray strands of wire to short to the adjoining position on the connector. b. Plug the connector into the 12-pin connector on the ICS-1000 rear panel. Doc. 031879-01 3/03...

  • Page 181: Selecting Ttl Input Functions And Control Types

    C • TTL and Relay Control c. Connect the wires from the ICS-1000 connector plug to the TTL or relay connector pins on the other module(s). Additional connector plugs are provided with other Dionex modules. NOTE Check the polarity of each connection. Connect signal wires to signal (+) pins and ground wires to ground (-) pins.
  • Page 182 Normal Edge the output signals provided by Dionex modules. If the device connected to the ICS-1000 does not send a normal edge signal, select the appropriate control type. Refer to the documentation provided with the controlling device and the information below to select the correct type.

  • Page 183: Controlling Ttl And Relay Outputs

    C.2 Controlling TTL and Relay Outputs The ICS-1000 provides two TTL outputs and two relay contacts for control of functions in external devices, such as an integrator or autosampler. The relay outputs can be used to switch any low-voltage control. Switched current must be less than 200 mA and 60 V peak blocking.
  • Page 184 ICS-1000 Ion Chromatography System The TTL and relay output states can be controlled, either by issuing direct control commands from the Chromeleon control panel or by including the commands in a control program. Figure C-4 shows an example relay program command for controlling an AS40 autosampler.

  • Page 185: Example Setup For Stand-Alone Operation

    Verify the AS50 Configuration Refer to Section B.17.1 to verify that the AS50 meets the configuration requirements for use with the ICS-1000. Connect the AS50 Autosampler Follow the instructions in Section B.17.2 to connect the AS50 autosampler to the ICS-1000 injection valve.
  • Page 186 RELAY IN RELAY OUT Third-Party Analog-to-Digital Converter and Controller Connection System Function AS50 TTL Out 1 to ICS-1000 TTL In 1 Inject AS50 TTL Out 2 to ICS-1000 TTL In 2 Autozero AS50 RLY Out 2 to ICS-1000 TTL In 3...
  • Page 187 Help Prompt Figure C-7. AS50 Time Function Out Screen 2. Turn the AS50 power off and then back on. You can now issue commands from the AS50 to switch the ICS-1000 injection valve position. Connect the Analog Output 1. Locate the analog cable for the third-party analog-to-digital converter and controller.
  • Page 188 RLY2 remains at 1 to keep the pump on. • TTL1 does not need to be set in the timed events because the Load and Inject commands control the ICS-1000 injection valve automatically (see “Enable AS50 Control of the Injection Valve” on page C-9).

  • Page 189: D • Reordering Information

    D • Reordering Information Part Number Item Reservoirs 2-L plastic reservoir assembly 046548 (includes stopper and cap) 039164 4-L plastic reservoir assembly (includes stopper and cap) Pump 057937 Primary pump head assembly 057938 Secondary pump head assembly 045721 Outlet check valve assembly, 10-32 045722 Inlet check valve assembly, 1/4-28 045994...
  • Page 190 ICS-1000 Ion Chromatography System Part Number Item 056118 CAES Cation Atlas Electrolytic Suppressor 053946 ASRS-Ultra 4-mm Anion Self-Regenerating Suppressor 053949 CSRS-Ultra 4-mm Cation Self-Regenerating Suppressor 057985 DS6 heated conductivity cell 058060 Column heater assembly 059979 Column heater heat exchanger, 0.25-mm (0.010-in) ID tubing 060943 Column heater heat exchanger, 0.125-mm (0.005-in) ID tubing...

  • Page 191: E • Faq

    E • FAQ How do I hook up an autosampler? For instructions on how to connect the ICS-1000 to an AS50 autosampler, see Section B.17 of this manual. To connect an AS40, see Section B.18. Also see the AS50 Autosampler Operator’s Manual (Document No. 031169) or the AS40 Automated Sampler Operator’s Manual (Document No.

  • Page 192: When Should I Remake Standards

    E.10 How do I shut off the system? In Chromeleon, click the System Shutdown button on the ICS-1000 Control Panel (see Figure 2-8). On the ICS-1000, turn off the power switch on the rear panel (see Figure 2-5). E.11 How do I store columns? Columns should be stored in eluent.

  • Page 193: How Do I Know When A Column Is Dirty

    • The suppressor needs regeneration. See the suppressor manual for troubleshooting information. The suppressor manual is included on the Dionex Reference Library CD-ROM (P/N 053891), located in the ICS-1000 Ship Kit (P/N 057905). • The cell is out of calibration. See Section 5.1.2...
  • Page 194 ICS-1000 Ion Chromatography System Doc. 031879-01 3/03...
  • Page 195 F • Glossary Analytical Column Synonymous with Separator Column. Band Spreading The broadening of the sample band as it travels through the column. Band spreading can also occur in the injection valve, detector cell, and interconnecting tubing. Calibration Curve A graph showing detector response in peak height or area versus analyte concentration.
  • Page 196 ICS-1000 Ion Chromatography System Cell Constant (k) A factor determined experimentally by measuring the conductance (G) of a standard solution of known equivalent conductivity (k). κ G ⁄ The value of k depends upon the surface area of, and distance between, the electrode faces in the conductivity detector cell.
  • Page 197 F • Glossary of relatively clean sample. This allows large volumes of sample to be injected, lowering concentration detection limits. Conductivity A measure of the ease with which electrical current flows through a liquid contained between two opposite charged electrodes. Conductivity is a characteristic of ions in solution.
  • Page 198 ICS-1000 Ion Chromatography System Packing The material that fills a chromatographic column; usually a resin or silica-based material. Pellicular Resin A resin with a solid, nonporous core coated with a thin layer of more porous material. The exchange sites of pellicular ion exchange resins are located only on the surface layer of the bead.
  • Page 199 F • Glossary Siemens (S) Unit measure of conductance; the reciprocal of the electrical resistance of a solution. Suppressor A device used to minimize eluent conductivity and convert sample species to a common form, thus increasing detection sensitivity. Temperature Coefficient The percent of change in the conductivity of a solution with a 1 C change in temperature.
  • Page 200 ICS-1000 Ion Chromatography System Doc. 031879-01 3/03...
  • Page 201 Index Air particulate samples, 3-12 Background conductivity Air regulator, B-47 – B-48 High, 4-18 Alarm conditions, 4-3 – 4-8 Offsetting, 3-11 See also Error messages Backpressure, 2-23 Ambient temperature, 4-19 Restriction in plumbing, 5-7 Analog output, C-2 Troubleshooting, 4-15 Connections, C-9 Baseline Connector, 2-7 Drift, 4-19...
  • Page 202 ICS-1000 Ion Chromatography System Check valves Commands Cleaning procedure, 5-11 – 5-12 Chromeleon, 2-11, 3-19, B-34 Replacement procedure, 5-11 – 5-12 Component panel, 2-4 Chromatogram, 1-3 Computer Chromeleon, 2-11 Connections, B-9 Adding devices to a timebase, B-16 Operating system requirements, B-5...
  • Page 203 Equilibration time, 3-11 Temperature compensation, A-3 Error messages, 4-3 – 4-8 Diagnostics, 5-1 Column heater exceeds safe temperature, Dimensions, A-1 Dionex Column heater open circuit, 4-3 Contacting, 5-1 Column heater short circuit, 4-3 Direct control, 2-11 Degas calibration failed, 4-4...
  • Page 204 ICS-1000 Ion Chromatography System Flow rate, 2-5, A-2 Leaking, 4-10 Calibration, 5-6 Plumbing connections, 2-24 Troubleshooting, 4-14 Rebuilding, 5-10 Flow schematic, 2-9 Specifications, A-3 FROM PC Troubleshooting, 4-5 USB connections, B-9 Installation, B-1 – B-53 Front panel, 2-1 Adding devices to a timebase, B-16...
  • Page 205 Index Key code (Chromeleon), B-6 Nitrite in samples, 3-12 Normal edge TTL input control, C-4 Normal pulse TTL input control, C-4 Leak sensor, 2-5 Leak sensor wet alarm, 4-4 Replacement, 5-28 Offsetting background conductivity, 3-11 Leaks Operating features, 2-1 Cell, 4-10 Operating pressure, A-1 Fittings, 4-9 Operating ranges...
  • Page 206 ICS-1000 Ion Chromatography System With a syringe, B-36 See Computer With isopropyl alcohol, 5-29 Peak ghosting, 4-16 With the Prime button, B-38 Peak height Priming valve Reproducibility, 2-27 Opening, 2-22 Troubleshooting, 4-17 O-ring replacement procedure, 5-18 Peaks Printing, E-1 Extraneous (ghosting), 4-16 Processing samples, 3-2, 3-18 –...
  • Page 207 Index Samples Collecting and storing, 3-12 Rear panel, 2-7 Diluting, 3-13 Analog output connector, 2-7 Filtering, 3-12 Power receptacle, 2-8 Injecting, 3-14, 3-16 USB connections, 2-7 Loading, 3-14 Rebuilding the injection valve, 5-10 Pretreating, 3-12 Relay connections Saving data, 3-19 AS40, B-44 Schematic Rear panel connector, 2-8, C-1...
  • Page 208 ICS-1000 Ion Chromatography System Sharable device, B-16 System properties dialog box, B-16 Software license, B-5 – B-6 Specifications Column heater, A-4 Conductivity cell, A-3 Technical Support, 5-1 Electrical, A-1 Temperature Environmental, A-1 Cell heater, 2-27 Injection valve, A-3 Column heater, 2-5, 2-26...
  • Page 209 Index TTL outputs Waste valve, 2-22 Controlling, C-5 Opening, 2-22, 5-13 Rear panel connector, 2-8 O-ring replacement procedure, 5-18 Tubing Water samples, 3-12 Replacing, 5-9 Weekly maintenance, 3-21 Requirements, 5-9 Weight, A-1 Routing clips, 2-8 Wellness Panel, 2-13, 5-1 Tubing connections Isolating a restriction, 4-6, 5-7 TUV, 1-8 Yearly maintenance, 3-22...
  • Page 210 ICS-1000 Ion Chromatography System Index-10 Doc. 031879-01 3/03...

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