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Dionex UltiMate 3000 Series Operating Instructions Manual

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UltiMate 3000 Series
Column Compartments
TCC-3000SD and TCC-3000RS
Operating Instructions
Revision: 1.0
Date: August 2008
© 2008 Dionex

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  • Page 1 UltiMate 3000 Series Column Compartments TCC-3000SD and TCC-3000RS Operating Instructions Revision: 1.0 Date: August 2008 © 2008 Dionex...
  • Page 2 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Operating Instructions...

  • Page 3: Declaration Of Conformity

    Declaration of Conformity Product: Column Compartment Types: TCC-3000SD and TCC-3000RS Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Equipment Directive 73/23/EEC changed by 93/68/EEC • EMC Directive 2004/108/EG The electrical safety of the products was evaluated based on the following standard: •...
  • Page 4 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Table of Contents Introduction........................1 Operating Instructions ................... 1 Safety Information ....................2 1.2.1 Symbols on the TCC and in the Manual ............2 1.2.2 General Safety Precautions................3 1.2.3 Consignes Générales de Sécurité..............5 Intended Use ......................
  • Page 6 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5.3 Configuring the TCC ..................32 3.5.3.1 Initial Installation..................32 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings ..34 Preparation for Operation (Startup) ................37 Overview of Actions ....................37 General Precautions for Connecting Capillaries..........38 Opening the Column Chamber ................39...
  • Page 7 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Special Chromeleon Functions ................73 5.6.1 Predictive Performance .................. 73 5.6.2 Diagnostics Tests.................... 74 5.6.3 Recording the Temperature Signals ............... 75 5.6.4 Operational Qualification und Performance Qualification......75 Shutting Down the TCC ..................75 Routine and Preventive Maintenance ..............
  • Page 8 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page iv Operating Instructions...

  • Page 9: Introduction

    The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of the TCC, Dionex recommends that you review the manual thoroughly before beginning operation.

  • Page 10: Safety Information

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 1.2 Safety Information The CE Mark label on the instrument indicates that the TCC is compliant with the related standards (→ page I). 1.2.1 Symbols on the TCC and in the Manual...

  • Page 11: General Safety Precautions

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Important: Indique que ne pas tenir compte de l'information jointe peut conduire à de faux résultat ou endommager l'instrument. Warning: Indicates that failure to take note of the accompanying information may result in personal injury.
  • Page 12 Disconnect the TCC from all power sources before removing the panels. When the panels are removed, dangerous electrical connections will be exposed. • Always replace blown fuses with original spare part fuses from Dionex (→ page 98). • Replace faulty power cords and communication cables.

  • Page 13: Consignes Générales De Sécurité

    Before interrupting operation for several days or more or when preparing the TCC for transport, observe the precautions for shutting down the TCC (→ page 75). • Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex accessories may impair the performance of the TCC. •...
  • Page 14 • Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex (→ page 98). • Remplacez les cordons d'alimentation électrique et les câbles de communication défectueux.
  • Page 15 75. • Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument. •...

  • Page 16: Intended Use

    96). If there is any question regarding appropriate usage, contact Dionex before proceeding. Dionex cannot be held liable for any damage, material or otherwise, resulting from inappropriate or improper use of the TCC. 1.4 Federal Communications Commission (FCC) Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S.

  • Page 17: Overview

    2 Overview 2.1 Unit Description The TCC is a modern high-quality instrument designed for HPLC analysis, especially as part of the Dionex UltiMate 3000 system. The design has been optimized for minimum dead volume and maximum efficiency. • Column thermostatting in a temperature range from +5 °C up to +110 °C (TCC-3000RS) or +5 °C up to +70 °C (TCC-3000SD) and the wide range of supported column switching...
  • Page 18 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • If required, the following components can be installed in the TCC: ♦ Pre-column heater (→ page 19) The pre-column heater brings the solvent and the sample to the column temperature before they enter the column. This avoids temperature gradients on the column.

  • Page 19: Operating Principle

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.2 Operating Principle The fundamental requirement for a column compartment for liquid chromatography applications is the ability to maintain the preset temperature as precisely as possible. However, for temperature equalization, temperature stability is more important than the absolute precision of the setting.

  • Page 20: Tcc Configurations

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.3 TCC Configurations The TCC is available in the following configurations: Column Compartment Part No. TCC-3000RS Column Compartment with post-column thermostatting 5730.0000 Basic version without column switching valves TCC-3000SD Column Compartment without post-column 5730.0010...

  • Page 21: Front Panel Elements

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.4 Front Panel Elements Fig. 1: Front panel view (example) Front Panel Description Element Display Shows information about the TCC, for example: - General information upon power-up (→ page 51) - Status screen (→ page 52) - Functions and menus (→...

  • Page 22: Rear Panel

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.5 Rear Panel Fig. 2: Rear panel view Description Power switch (→ page 15) Fuse cartridge (→ page 15) Main power receptacle (→ page 26) Type label Digital I/O ports for communication with external devices under Chromeleon (→ page 15) USB (Universal Serial Bus) port for connection to the Chromeleon computer (→...

  • Page 23: Power Switch

    The 6-pin Mini-DIN ports on the rear panel can be used in Chromeleon to exchange digital signals with external devices. An appropriate connection cable (6-pin Mini-DIN signal cable) is available from Dionex (part no. 6000.1004). For information about how to how to connect the digital I/O, see page 27.

  • Page 24: Interior Components

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.6 Interior Components Fig. 3: TCC interior view Description Column switching valve It depends on the TCC configuration whether a valve is installed in this position (→ page 12). Slots for the column ID chip cards (→ page 68) Column switching valve It depends on the TCC configuration whether a valve is installed in this position (→...

  • Page 25: Gas And Humidity Sensors

    (→ page 62). 2.8 Column Switching Valve A wide range of column switching valves are available from Dionex for the TCC (→ page 12). They allow you to realize most applications in HPLC and U-HPLC. Fig. 4 through Fig.
  • Page 26 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • A 2-position, 10-port valve is used for applications that require overlapping injections with offline re-equilibration of the column. Column 2 Column 2 Waste Waste Injector Injector Detector Detector From column re-...

  • Page 27: Column Identification System (Column Id)

    2 µL or 7 µL. • If you want to increase the flow rate to 2 mL/min or if the temperature difference is more likely to be 30 °C, Dionex recommends installing an pre-column heater with a volume of 11 µL. Operating Instructions...

  • Page 28: Post-Column Cooler

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.11 Post-Column Cooler Only TCC-3000RS The TCC-3000RS supports post-column cooling. A post-column cooler insert with a thermally effective volume of 2 µL (total volume: 5 µL) is shipped in the accessories kit for the TCC-3000RS.

  • Page 29: Chromeleon Software

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.12 Chromeleon Software The TCC can be controlled by the Chromeleon Chromatography Management System. To control the TCC, an appropriate Chromeleon version and a Timebase Class 1 Chromeleon license are required. Two modes of software control are available: •...
  • Page 30 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 22 Operating Instructions...

  • Page 31: Installation

    Tips: Immediately report any shipping damage to both, the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately. Keep the original shipping container and packing material. They provide excellent protection for the TCC in case of future transit.

  • Page 32: Positioning The Tcc In The Ultimate 3000 System

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3. Remove the foam spacers. 4. Before connecting the TCC to the power source, wait approximately 4 hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate.
  • Page 33 Fig. 9: Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer. However, Dionex recommends interconnecting all modules, and then connecting the system to the Chromeleon computer via only one connection.

  • Page 34: Connecting The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.4 Connecting the TCC 3.4.1 General Information If you want to operate the TCC with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the TCC to the USB port on the Chromeleon computer and turn on the TCC power.

  • Page 35: Connecting The Digital I/O

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.4.4 Connecting the Digital I/O To connect an external device to the digital I/O ports on the rear panel, use the appropriate 6-pin mini-DIN cable (part no. 6000.1004). 1. Plug the 6-pin connector of the mini-DIN cable into the Digital I/O port 1 (or 2).
  • Page 36 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The table lists the functions assigned to the connector pins and the color and label of the cable wire connected to each pin. Signal Name Signal Level Wire Color Wire Label Not used...

  • Page 37: Setting Up The Tcc In Chromeleon

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5 Setting Up the TCC in Chromeleon This section provides brief instructions for setting up Chromeleon. For details, see the Chromeleon Help. Tip: When the TCC is connected to the Chromeleon computer, verify that the Chromeleon software is installed before turning on the TCC power for the first time.
  • Page 38 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments c) Install Chromeleon. d) Turn on the power to the TCC. Windows Vista will now detect the TCC and install the USB software for the TCC automatically. Windows XP and 2000 will automatically detect the new TCC and launch the Found New Hardware Wizard, which guides you through the USB installation.

  • Page 39: Installing The Tcc

    5. Open the Add device to timebase dialog box. To do so, click Add Device on the Edit menu or right-click the timebase and click Add Device on the menu. 6. On the Manufacturers list, click Dionex HPLC: UltiMate 3000 and on the Devices list, click TCC-3x00(RS) Column Compartment.

  • Page 40: Configuring The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5.3 Configuring the TCC 3.5.3.1 Initial Installation During the installation, Chromeleon connects to the TCC and transfers the settings from the instrument firmware to Chromeleon, setting the options on the wizard pages accordingly.
  • Page 41 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • Firmware Download Click this button to update the TCC firmware with the version available in Chromeleon. (The button appears dimmed if the Demo Mode is enabled.) The TCC is shipped with the most recent firmware version. If a firmware update is ever required, follow the steps in section 7.5 (→...

  • Page 42: Changing The Basic Configuration Or Selecting Advanced Settings

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • Column A through Column D The TCC supports column identification (→ page 19). Select the columns for which you want to enable column identification. The entry field behind the column displays the name used to identify the column in the installation environment and in the Chromeleon client.

  • Page 43: Configuration Page

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Configuration Page Use this page to determine whether Chromeleon shall record the temperature signals as a separate channels and select the pressure unit. Fig. 15: Configuration page (here TCC-3000RS) • Temperature Signal The check box is selected by default.
  • Page 44 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Relays Page The Relays page lists all available relays. Select a check box to enable the corresponding relay. If a check box is cleared, the relay will not be available in Chromeleon. You can change the name and timebase assignment for a relay.

  • Page 45: Preparation For Operation (Startup)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4 Preparation for Operation (Startup) 4.1 Overview of Actions After you have unpacked, positioned and connected the TCC as described in sections 3.1 through 3.4, prepare the TCC for operation. Follow the sequence of steps below. When connecting the capillaries, observe the precautions on page 38.

  • Page 46: General Precautions For Connecting Capillaries

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.2 General Precautions for Connecting Capillaries When connecting capillaries, observe the following general precautions: • Make sure that the connectors are free from contaminants. Even minute particles may cause damage to the system.

  • Page 47: Opening The Column Chamber

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.3 Opening the Column Chamber The column chamber in the TCC is easily accessible. The front panel tilts downward when you unlock the door as shown in the picture. Fig. 17: Unlocking the front panel door Important: Do not place any heavy objects on the open front panel door.

  • Page 48: Inspecting The Valve Drainage

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.4 Inspecting the Valve Drainage The column switching valves are fitted with a drip tray for valve drainage. Liquid leaks from the valves are discharged to waste, together with liquid leaks that might have accumulated in the interior of the TCC.

  • Page 49: Installing A Separation Column

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.6 Installing a Separation Column Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal or plastic parts inside the column chamber while the temperature set point is >...
  • Page 50 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3. If you want to use column identification Attach the column ID chip card to the column. Two chip cards are provided in the TCC accessories kit. Wrap the ribbon around the column, pass the shank of the rivet through a hole, and press down to unite the two parts.

  • Page 51: Installing The Post-Column Cooler Insert

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.7 Installing the Post-Column Cooler Insert Only TCC-3000RS The post-column cooler insert is installed under the column chamber. A removable panel on the front of the TCC provides easy access to the installation section.

  • Page 52: Connecting The Components To The Column Switching Valve

    To avoid damage to the biocompatible column switching valve, use only the PEEK fitting nuts and ferrules from the TCC accessories kit or use original Dionex spare parts. Do not use metal fittings and ferrules for the connection. They can cause damage to the valve.

  • Page 53: Connections On A 2-Position, 6-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments To ensure proper connection, a short part of the capillary must stick out of the ferrule. Therefore, remove the capillary from the valve to verify this. In addition, check that the ferrule grabbed the capillary. When pulling the ferrule gently, you should not be able to move the ferrule on the capillary.

  • Page 54: Connections On A 2-Position, 10-Port Column Switching Valve

    10. For a micro setup, install a capillary with an inner diameter of 0.13 mm. For an analytical setup, the capillary should have an inner diameter of 0.25 mm. 3. Ports 8 and 9 are not used for this setup. Dionex recommends connecting these ports with an additional capillary or closing them with a plug.

  • Page 55: Connections On A 6-Position, 7-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.8.3 Connections on a 6-position, 7-port Column Switching Valve Fig. 30 shows an example for the connections on a 6-position, 7-port column switching valve. Connect … Column 1 Column 2 Column 3...

  • Page 56: Equilibrating The System

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.10 Equilibrating the System Before using the TCC for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition.
  • Page 57 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The equilibration panel shows the equilibration status of each instrument in the system. Fig. 32: Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments.
  • Page 58 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 50 Operating Instructions...

  • Page 59: Operation And Maintenance

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5 Operation and Maintenance The TCC can be controlled by the Chromeleon Chromatography Management System. For details, see section 5.3 (→ page 53). In addition, function keys and menus are available on the TCC display to facilitate operation during, for example, initial installation of the TCC, diagnostics, and maintenance.

  • Page 60: Status Screen

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.2 Status Screen When the self test was successful, the initial screen changes to the status screen. The TCC determines the appearance of the screen. Fig. 33 shows the status screen for a TCC with column switching valves. For a TCC without column switching valves, the appearance of the screen is similar to status screen shown in Fig.

  • Page 61: Operation With Chromeleon

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.3 Operation with Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The TCC is connected to the Chromeleon computer via a USB connection.

  • Page 62: Direct Control

    To open the Commands dialog box for the TCC 1. Open a control panel (any panel is possible). To open a control panel, open the Chromeleon Browser and double-click a control panel in the Dionex Templates/Panels folder. 2. Connect the control panel to the timebase in which the TCC is installed. On the Control menu, select Connect to Timebase, and then select the timebase on the Timebase tab.
  • Page 63 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The commands and parameters available in the dialog box vary, depending on the ♦ Chromeleon version ♦ options selected for the TCC in the Properties dialog (→ page 32). ♦ display filter level (Normal, Advanced, or Expert) 5.
  • Page 64 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2. On the Panel Tabset for your timebase, click the Col. Comp page. 3. Verify that the TCC is connected to Chromeleon (the LED next to the Connect button is green). If it is not, click Connect.

  • Page 65: Automated Control

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.3.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the TCC. Programs can be created automatically with the help of a software wizard or manually by editing an existing program. In addition to programs for sample analysis, you...
  • Page 66 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 35). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.

  • Page 67: Display Screens (Function Keys And Menus)

    Four white spots on the front panel mark the positions of four function keys: Menu, Temp, Valve, and Leak. To show the keys, select the position of the utmost left spot on the TCC display with the Dionex menu pen (part no. 6300.0100). The menu pen is included in the TCC shipment.

  • Page 68: Tcc Menus

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments To … Select … Menu Open the Main menu (→ page 62). Temp Open the Temperature menu (→ page 63). Valve Open the Valve menu (→ page 63). Leak Open the Leak menu (→ page 63).

  • Page 69: General Menu Layout And Structure

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2.1 General Menu Layout and Structure In general, the menu layout is as follows: > Control (1..3) 1. Temperature 2. Valve 3. Leak ∨ ∧ Select Back Fig. 39: Menu layout (here Control menu) Tubing from Reports the menu name and the number of items on the menu list.

  • Page 70: Main Menu

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments To … Select … Toggle between two operating states (for example, between On and Off). Toggle Confirm the selection or input. Cancel the action and restore the last value. Cancel Note: Depending on the selected option, specific keys may replace these general keys.

  • Page 71: Control Menu

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2.3 Control Menu On the Control menu, you can make the settings for temperature control and valve control. In addition, you can activate leak detection and specify the sensitivity of the gas and humidity sensors.

  • Page 72: Information Menu

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2.4 Information Menu The Information menu provides information for diagnostic purposes (read-only). > Information (1..3) 1. System 2. Valve 3. Temperature work load ∨ ∧ Select Back Fig. 42: Information menu To …...

  • Page 73: Configuration Menu

    Back Fig. 43: Configuration menu To … Select … Restore the Dionex standard settings. In the Reset to factory defaults? Reset to factory defaults dialog box, select OK to confirm the restore or select Cancel to keep your settings. Set the display and function key parameters: Display &...

  • Page 74: Operational Settings

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5 Operational Settings 5.5.1 Selecting the Column For a TCC with one or two column switching valves, column programming is performed in Chromeleon. The number of column switching valves, the valve model, and the application determine which commands and properties are used.

  • Page 75: Turning On Column Thermostatting

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.2 Turning On Column Thermostatting You can turn column thermostatting on and off and set the desired temperature in Chromeleon or on the TCC display. To turn on column thermostatting from Chromeleon 1.

  • Page 76: Activating Column Identification (Column Id)

    1. Verify that the column ID chip card is attached to the column (→ page 42). 2. Insert the chip card, with the Dionex logo facing up, into one of the four chip card readers (A - D). (The chip card reader at the utmost left is the reader for column A; the chip card reader at the utmost right is the reader for column D.) Four chip cards can be...

  • Page 77: Adjusting The Sensitivity Of The Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • Operational column properties Chromeleon determines and updates the operational information upon each injection. The operational column properties are, for example, the date of the first injection, the date of the last injection, the maximum flow rate while the column is in use, or the total volume of sample that has been injected onto the column.

  • Page 78: Turning On Post-Column Thermostatting

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.5 Turning on Post-Column Thermostatting Only TCC-3000RS You can turn post-column thermostatting on and off and set the desired temperature in Chromeleon or on the TCC display. To turn on post-column thermostatting from Chromeleon 1.

  • Page 79: Adjusting The Screen Brightness Or Contrast

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.6 Adjusting the Screen Brightness or Contrast You can adjust the screen brightness or screen contrast to your requirements from Chromeleon or on the TCC display. To adjust the settings from Chromeleon 1.

  • Page 80: Smartstartup And Smartshutdown

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.8 SmartStartup and SmartShutdown The SmartStartup wizard assists you in automating regular routine tasks (→ page 48). With SmartStartup, the different modules of the UltiMate 3000 system are turned on automatically and in a controlled manner. For example, for the TCC, the column chamber and all components inside are brought to the desired temperature.

  • Page 81: Special Chromeleon Functions

    Open the Commands dialog box for the TCC and enter the limits for the predictive performance parameters. For a list of the commands and counters that are supported for the TCC, see the Chromeleon Help. To keep the predictive performance information up-to-date, Dionex recommends the actions listed in the table: After you have …...

  • Page 82: Diagnostics Tests

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The color-coding of the wellness bars provides information about the status: Color Description Green Yellow The value will soon reach the specified limit and/or the related component needs servicing or should be replaced soon.

  • Page 83: Recording The Temperature Signals

    Components page (→ page 33). Dionex recommends recording the temperature signals in a program. If a problems occurs, the temperature signal channels can provide helpful information to identify and eliminate the problem.
  • Page 84 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments If you are running Chromeleon, you can set the TCC and HPLC system into the standby mode or automate system shutdown (see below). Standby Program A standby program sets the HPLC system into standby mode. The application can be reactivated very quickly afterward.

  • Page 85: Routine And Preventive Maintenance

    Check the volume of the liquid in the waste container and empty as needed. After using saliferous buffers, rinse the fluid connections with de-ionized water. Anually Have a Dionex service representative check the TCC once a year to prevent, for example, contamination and excessive wear. Tip:...
  • Page 86 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 78 Operating Instructions...

  • Page 87: Troubleshooting

    Chromeleon Diagnostics Messages section for a short description of possible causes along with recommended courses of action (→ page 83). If you are unable to eliminate a problem following the instructions given here, contact Dionex Service. Operating Instructions...

  • Page 88: Messages On The Tcc Display

    The table lists TCC-related messages along with appropriate remedial actions. In addition to the messages listed in the table, other messages may appear. In this case, note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem.
  • Page 89 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Message Remedial Action Error while programming Turn the TCC off and on again by pressing the power switch on the flash! rear of the TCC and retry. (This message may be extended by additional text.)
  • Page 90 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Message Remedial Action Right valve controller Turn the TCC off and on again by pressing the power switch on the configuration error! rear of the TCC and retry. Right valve moving timeout Check whether the spanner has been tightened too firmly.

  • Page 91: Chromeleon Diagnostics Messages

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6.3 Chromeleon Diagnostics Messages If the TCC fails a diagnostics test, perform the instructions given here. If the TCC still fails the test, contact Dionex Service. Gas Sensor Test failed Test Result Probable Cause...
  • Page 92 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Test Result Probable Cause Remedial Action Timeout waiting for The thermal unit may be defective. Repeat the test. temperature to reach (exceed, approach) room temperature during heating (or cooling) phase. Page 84...

  • Page 93: Operating Problems

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6.4 Operating Problems The table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information, also see the manuals for the other modules of an UltiMate 3000 system.
  • Page 94 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Problem Probable Cause Remedial Action The temperature does not The ambient temperature is too Reduce the ambient change for some time high or the set point is lower than temperature (for example, by...

  • Page 95: Service

    For more information, see the warranty statement in the terms of sale. • Before returning a TCC to Dionex for repair, contact Dionex Service or your local distributor. An RMA (Return Material Authorization) number is required to track your instrument.

  • Page 96: Removing Gases And Humidity From The Column Chamber

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.2 Removing Gases and Humidity from the Column Chamber A gas sensor and a humidity sensor are installed inside the TCC. The sensors detect any gas or humidity that may accumulate in the column chamber. When a certain concentration...

  • Page 97: Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3 Column Switching Valve The TCC can hold one or two column switching valves (→ page 12). To ensure optimum operation of the valve, observe the following guidelines: ® • All parts of the valve that are exposed to solvents are made of Vespel and DuraLife™...

  • Page 98: Inspecting The Column Switching Valve For Leakage

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.1 Inspecting the Column Switching Valve for Leakage Several drain holes are provided on the stator ring. If liquid droplets appear on one or more drain holes, this may indicate valve leakage.

  • Page 99: Replacing The Valve Pod

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.2 Replacing the Valve Pod Valve pod* for Part No. TitanHP™ column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port switching valve** 6722.9013 6-position, 7-port switching valve** 6722.9035 Suitable for pressures <...
  • Page 100 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments TitanHT Column Switching Valve 4. Unscrew and remove the locking ring (spanner) using only our hand. Do not use a wrench. Spanner Fig. 50: Removing the spanner 5. Grasp the pod and pull out firmly to remove the pod from the valve driver body.

  • Page 101: Troubleshooting Tip

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Troubleshooting Tip If you cannot push the pod completely into the valve driver body, the position of the valve coupling does not match the position of the valve drive. To resolve the problem: 1.
  • Page 102 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5. Push the pod completely into the valve driver body. Be sure to install the pod in the correct orientation. Align the large slot in the pod with the matching runner in the valve driver body.

  • Page 103: Replacing The Valve Stator

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.3 Replacing the Valve Stator Stator for Part No. TitanHP™ column switching valves Suitable for pressures < 413 bar (6000 psi) 2-position, 6-port switching valve 6722.9011 6-position, 7-port switching valve 6722.9031 Suitable for pressures < 345 bar (5000 psi) 2-position, 10-port switching valve 6722.9021...

  • Page 104: Rotor Seal And Stator Face Seal

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.4 Rotor Seal and Stator Face Seal The valve type, pressure range, and the pH value of the application determine the type of seals: Description Part No. ® Rotor Seal (RheBuild ) kit forTitanHP™...

  • Page 105: Replacing The Rotor Seal

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.4.1 Replacing the Rotor Seal 1. Remove the stator screws located in the stator (→ Fig. 55, page 95), and then remove the stator from the valve body. 2. Slip a flathead screwdriver underneath the rotor seal and remove the seal.

  • Page 106: Replacing The Main Power Fuses

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.4 Replacing the Main Power Fuses Warning: Before replacing the fuses, turn off the TCC. Be sure to disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez l'instrument. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur.

  • Page 107: Updating The Tcc Firmware

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.5 Updating the TCC Firmware The TCC is shipped with the most recent firmware version. The TCC firmware is also included in Chromeleon. To check which firmware version is installed in the TCC and which version is included in Chromeleon: •...
  • Page 108 If the download is not successful, the related messages appear in the Audit Trail. In this case, repeat the above steps. If this download is also not successful, turn the TCC off and on again, and repeat the download. If the download fails again, contact Dionex Service. Page 100...

  • Page 109: Technical Information

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 8 Technical Information Temperature range: 5 °C to 110 °C, max. 18 °C below ambient TCC-3000RS 5 °C to 70 °C, max. 18 °C below ambient TCC-3000SD Temperature accuracy ±0.5 °C (Column chamber): Temperature Stability ±0.1 °C...
  • Page 110 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Environmental conditions: Range of use: Indoor use Temperature: 10 °C to 35 °C (50 to 95°F) Air humidity: 80% relative humidity, non-condensing Overvoltage category: II Pollution degree: Dimensions (h × w × d): 19 x 42 x 51 cm (7.5 x 16.5 x 20 inch)

  • Page 111: Accessories, Consumables, And Spare Parts

    USB cable, type A to type B, high speed USB 2.0 6720.8910 (cable length: 0.5 m) Assortment box for accessories 6000.0043 * The part numbers always refer to the packing unit. For more information, contact your Dionex sales representative. Operating Instructions Page 103...
  • Page 112 USB cable, type A to type B, high speed USB 2.0 6720.8910 (cable length: 0.5 m) Assortment box for accessories 6000.0043 * The part numbers always refer to the packing unit. For more information, contact your Dionex sales representative. Page 104 Operating Instructions...

  • Page 113: Optional Accessories

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 9.2 Optional Accessories Description Part No. Column switching valves To fit the TCC with column switching valves, you need the appropriate valve drive (actuator) and pod. Valve drive (actuator) for TitanHP™ column switching valve, suitable for pressures <...

  • Page 114: Consumables And Spare Parts

    TCC-3000SD and TCC-3000RS Column Compartments 9.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact your Dionex sales representative. Description Part No.* Allen wrench (2.5 mm) 6146.2625...
  • Page 115 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Description Part No.* Pod for biocompatible TitanHP™ valves (PEEK), for pressures < 345 bar (5000 psi) 2-position, 6-port switching valve (PEEK) 6723.9013 2-position, 10-port switching valve (PEEK) 6723.9023 The rotor seal in the valve pod is suitable for pH values 0 - 14.
  • Page 116 USB cable, type A to type B, high speed USB 2.0 (cable length: 1 m) 6035.9035 * The part numbers always refer to the packing unit. For more information, contact your Dionex sales representative. ** Availability upon request. Contact Dionex.

  • Page 117: Index

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 10 Index Configuration ............ 12 Dialog Box............ 35 Menu............62, 65 Accessories Connection Optional ............105 Chromeleon Computer........26 Standard ............103 Digital I/O............. 27 Signal Cable..........27 USB .............. 26 Back ..............61 Consumables ........... 106 Batch..............58...
  • Page 118 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Prev ............62, 80 Power-Up............51 Select.............61 Safety Precautions .......... 3 Temp .............60 Safety Symbols..........2 Toggle ............62 Status Screen ..........52 Valve.............60 TCC Display..........13 Fuse Cartridge ...........15 Operational Qualification ........75 Fuses ..............98...
  • Page 119 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Service ...............87 Screen Brightness ......... 71 Column Switching Valve ......90 Screen Contrast..........71 Firmware Update ..........99 Status Screen .......... 52, 71 General Notes ..........87 TCC Drainage ........... 47 Replace Fuses..........98 Technical Information ........101 Säulenschaltventil .........89...
  • Page 120 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 112 Operating Instructions...

This manual is also suitable for:

Ultimate tcc-3000sdUltimate tcc-3000rs

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