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Summary of Contents for Zeiss Viluma 5
- Page 1 Instruction Manual ZEISS Viluma 5, Viluma 7 Multicolor LED Light Source for Gentle Fluorescence Imaging...
- Page 2 Effective from: 10/2024 © 2024 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in- formation or filing system.
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Page 3: Table Of Contents
Cabling the Viluma 5/7 ..................22 Configuring the PC Connection ................23 4.6.1 CAN Connection: Automatic Configuration ........... 23 4.6.2 USB Connection: Configuring with MicroToolBox (MTB)......23 Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... - Page 4 8 Technical Data and Conformity ............37 Performance Data and Specifications..............37 Applicable Standards and Regulations ..............38 Declaration of China RoHS ................. 39 Revision History................40 Index ....................42 Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101...
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Page 5: About This Instruction Manual
1 About this Instruction Manual | 1.1 Text Conventions and Link Types 1 About this Instruction Manual This Instruction Manual (further called "document") is considered to be part of the Viluma 5 and Viluma 7 (further called "Viluma 5/7"), herein also after referred to as "product". -
Page 6: Explanation Of Symbols
Date of manufacture optionally given adjacent to this symbol WEEE label: Do not discard as unsorted waste. Send to separate collection facilities for recovery and recycling Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 7: Further Applicable Documents
Many of these instruction manuals can be found at the ZEISS Portal. 1.5 Contact If you have any questions or problems, contact your local ZEISS Sales & Service Partner or one of the following addresses: Headquarters Phone: +49 1803 33 63 34... -
Page 8: Safety
Carl Zeiss Microscopy GmbH, Jena, Germany 2.1 Intended Purpose The Viluma 5/7 is designed to be used as an LED light source for reflected light fluorescence appli- cations. It can be used as an accessory in connection with upright and inverted microscopes. -
Page 9: Safe Operating Condition
Ensure conditions and take measures to prevent the build up of electrostatic charge on the workplace. 2.2.3 Order and Use of Spare Parts Using spare parts that are not provided by ZEISS can be hazardous or can lead to property dam- age. §... -
Page 10: Prevention Of Hazards
2 Safety | 2.3 Prevention of Hazards ZEISS The following table lists the risk grouping of light emitted from the Viluma 5/7 in the given spec- tral range according to the mentioned standard: Spectral range Risk group Actinic UV Near UV... -
Page 11: Hazards Generated By Radiation
§ Immediately replace damaged or illegible warning labels. In case a label is missing, contact your ZEISS service representative for free of charge replacement. 2.4.1 Labels on the Viluma 5/7 Fig. 1: Warning and information labels on the Viluma 5/7 LED light source... - Page 12 Do not stare at operating lamp. May be harmful to the eyes. Hot surface! Do not touch. Patent label (only valid for Viluma 7) Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101...
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Page 13: Labels On The Control Panel (Optional)
Fig. 3: Warning and information labels on the LED trigger box Pos. Symbol Description Type label Note: Although the type label states Colibri 7, the LED trigger box is intended for Colibri 5, Colibri 7, Viluma 5, and Viluma 7. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 14: Product And Functional Description
§ Axiovert 5 § Axiovert 7 Info For additional information and detailed descriptions, refer to further applicable documents or ask your ZEISS Sales & Service Partner. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 15: Main Components
For information on suitable filter sets for the Viluma 5/7, refer to the Filter Assistant in the ZEISS Microscopy Webshop or ask your ZEISS Sales & Service Partner. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 16: Controls And Displays
3 Product and Functional Description | 3.2 Controls and Displays ZEISS 3.2 Controls and Displays 3.2.1 Control Panel (Optional) If the Viluma 5/7 is used with manual stands without TFT, it can be controlled via the control panel (optional). Fig. 6: Control panel Pos. - Page 17 Tab. 2: Functions of the control panel Default brightness setting and intensity value memory: § After initialization and activation of the LEDs: 20 % § After de-energizing the product: Default values are set. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101...
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Page 18: Led Trigger Box (Optional)
3 Product and Functional Description | 3.2 Controls and Displays ZEISS 3.2.2 LED Trigger Box (Optional) The LED trigger box is needed for fast control of the Viluma 5/7 via TTL and via analog signals from third-party software. Fig. 7: LED trigger box front and rear side... -
Page 19: Installation
2. Check them for completeness as per delivery note. 4.2 Cabling Diagram for Operation without PC Fig. 8: Cabling diagram for operation without PC Viluma 5/7 Control panel LED trigger box (optional) Desktop power supply Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 20: Cabling Diagram With Pc And Motorized Microscope
4.3 Cabling Diagram with PC and Motorized Microscope Fig. 9: Cabling diagram with PC and motorized microscope for fast acquisition Viluma 5/7 SVB 1 (optional, only for fast acquisi- tion) Desktop power supply Microscope Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 21: Installing The Viluma 5/7 At The Reflected Light Port
2. Attach the dovetail of the Viluma 5/7 to the reflected light port 3. Tighten the clamping screw The Viluma 5/7 does not require any adjustment. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 22: Cabling The Viluma 5/7
The Viluma 5/7 can be controlled either via the TFT display of the microscope or by the ZEN (blue edition) software on the PC. 3. If a CAN bus connection is not available at the microscope and PC control of the Viluma 5/7 is required: Connect the USB connection socket of the Viluma 5/7 via USB cable to the PC. -
Page 23: Configuring The Pc Connection
4.6.2 USB Connection: Configuring with MicroToolBox (MTB) If the Viluma 5/7 is connected to the PC via USB, the connection type and the type of the light source must be selected manually in the MTB (version 3.8.x or higher) and transferred to the hard- ware configuration. -
Page 24: Fig. 13 Mtb - Configuring The Light Source Type
à The button Write configuration to hardware will appear. 5. Click on Write configuration to hardware. 6. Close the MTB software. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 25: Operation
3. If used, start the control software (ZEN 3.10 or higher or third-party software). If the control panel is connected, it will be powered via the Viluma 5/7 and is also ready for use. The default brightness setting of each individual LED is 20 % when activating it for the first time after initialization. -
Page 26: Controlling Via The Control Panel
RL Illumination to Off. 5.3.2 Controlling via the Control Panel Prerequisite ü The Viluma 5/7 is attached to the microscope and powered via the desktop power supply unit. ü The control panel is connected to the Viluma 5/7. The microscope is switched on. - Page 27 100 %. When decreasing the brightness, the brightness of all LEDs will be decreased in reverse order. If more than two LEDs are active, the maximum intensity of each LED is 60 %. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101...
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Page 28: Controlling Via Zen
5.3.3.1 "Locate" Tab Fig. 15: Locate tab - Microscope Control tool Prerequisite ü The Viluma 5/7 is attached to the microscope, connected via CAN, and powered via the desk- top power supply unit. ü The microscope and the PC are switched on. - Page 29 ZEISS 5 Operation | 5.3 Controlling 6. For Viluma 5/7 models equipped with the G/Y LED (567 nm), select the desired excitation filter by clicking on the 555 or 590 button at Excitation Filter of LED 567. à The displayed wavelength in the Viluma/Colibri 5/7 control menu will change accord- ingly.
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Page 30: Fig. 16 Locate Tab - Camera Tool
Fig. 16: Locate tab - Camera tool Prerequisite ü The Increment connection socket of the Viluma 5/7 is connected to the trigger output of the camera. Refer to the operating manual of the camera. Procedure 1. Click on the Locate tab 2. -
Page 31: Fig. 17 Acquisition Tab - Channels Tool
5.3.3.2 "Acquisition" Tab: "Channels" Tool Fig. 17: Acquisition tab - Channels tool Prerequisite ü The Viluma 5/7 is attached to the microscope, connected via CAN, and powered via the desk- top power supply unit. ü The microscope and the PC are switched on. - Page 32 Thus, the bleaching impact on the sample will be reduced. All relevant shutters will be automatically controlled by ZEN. In an experiment, the Gated mode is typically faster than the Continuous mode. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101...
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Page 33: Fig. 18 Acquisition Tab - Acquisition Mode Tool
Fig. 18: Acquisition tab - Acquisition Mode tool Prerequisite ü The Increment connection socket of the Viluma 5/7 is connected to the trigger output of the camera. Refer to the operating manual of the camera. Procedure 1. Click on the Acquisition tab 2. -
Page 34: Switching Off The Viluma 5/7
ü Procedure 1. The control of the Viluma 5/7 in the Imaging Setup tool of the Acquisition tab is analo- gous to the Microscope Control tool. 2. Additionally, the switching states of the LEDs and the image acquisition mode can be saved here by clicking the check boxes in the Include in this Setting... -
Page 35: Care And Cleaning Work
Info Labels on the device may only be cleaned using a dry cloth. 3. Wipe off the area with the cloth. 4. Dry with a lint-free cloth. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 36: Decommissioning And Disposal
It must be disposed of in accordance with applicable regulations (WEEE Directive 2012/19/ EU). ZEISS has implemented a system for the return and recycling of devices in member states of the European Union that ensures suitable reuse according to the EU Directives mentioned. -
Page 37: Technical Data And Conformity
LEDs are used with maximal intensity and are continuously switched on. This could result in gradually darker images over the experiment time. Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 38: Applicable Standards And Regulations
* Given are the peak wavelengths of the excitation filters. 8.2 Applicable Standards and Regulations The Viluma 5/7 is a product for research purposes only. It conforms to current international stan- dards (e.g. IEC 61010-1) as well as to harmonized standards of the applied EU directives. -
Page 39: Declaration Of China Rohs
8.3 Declaration of China RoHS 关于电器电子产品有害物质限制说明 Microscopes and accessories of the Carl Zeiss Microscopy GmbH are in compliance with the China RoHS Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equip- ment (RoHS) SJ/T 11364 with respect to lead, mercury, cadmium, hexavalent chromium, poly- brominated biphenyls (PBB) or polybrominated diphenylethers (PBDE) content. -
Page 40: Revision History
Date of Introduced Modifications sion Issue § 10/2024 Corrections § 09/2024 Corrections § 11/2023 Adaptation from Colibri to Viluma § Implementation of UKCA marking § Editorial rework Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 41: List Of Figures
Fig. 17 Acquisition tab - Channels tool ................Fig. 18 Acquisition tab - Acquisition Mode tool ..............Fig. 19 Acquisition tab - Imaging Setup tool ............... Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... -
Page 42: Index
35 starting 23 Operation Prerequisites 25 Prerequisites Operation 25 Requirements for Operators 8 Safe Operating Condition 9 Safety 8 Shutdown 36 Software 7 Spare parts 9 Instruction Manual ZEISS Viluma 5, Viluma 7 | en-US | Rev. 3 | 423052-7021-101... - Page 44 Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 phone: +49 1803 33 63 34 07745 Jena fax: +49 3641 64 3439 Germany info.microscopy.de@zeiss.com www.zeiss.com/microscopy...
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