Summary of Contents for Beckman Coulter Allegra V-15R
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Instructions For Use Allegra V-15R Centrifuge PN C63130AF June 2022 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
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• In the UK, call us at +44 845 600 1345 • In Ireland, call us at +353 (01) 4073082 • In Italy, call us at +39 0295392 456 • In other locales, contact your local Beckman Coulter Representative. May be covered by one or more pat. - see www.beckman.com/patents.
Changes or additions were made to the following: UKCA symbol and address added to Copyright page (inside cover page); Revision History, Software Version; Safety Notice, Safety and Regulatory Symbols, Safety Symbols Used for Allegra V-15R Centrifuge; CHAPTER 1, System Description, Table 1.2 Specifications.
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Requirements. APPENDIX B: Storage and Transport, Transport Safety Device, Installation. APPENDIX C: Acceleration and Deceleration Profiles, Table C.1, Allegra V-15R Acceleration and Deceleration Profiles. Note: Changes that are part of the most recent revision are indicated in text by a bar in the left margin of the amended page.
If in doubt as to how to proceed in any situation, contact Beckman Coulter, Inc. urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory instrumentation.
It is your responsibility to decontaminate components of the instrument before requesting service by a Beckman Coulter Representative or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so.
Always use the appropriate Personal Protective Equipment (PPE) when cleaning the centrifuge. Observe the cleaning procedures outlined for the Allegra V-15R centrifuge in this manual. Prior to cleaning equipment that has been exposed to hazardous material, we recommend that you: •...
• Do not place containers holding liquid on or near the chamber door. If spilled, liquid may get into the centrifuge and damage electrical components. • The power line cord, for the Allegra V-15R, is the disconnecting device used to remove electrical power. Make sure there is adequate clearance around the centrifuge in order to reach the line cord.
• Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. • Never lean on the centrifuge or place items on it while it is operating. • If using the optional Allegra V-15R Mobile Cart, the castors must be locked prior to use. PN C63130AF...
• Dispose of all waste solutions according to environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter. PN C63130AF...
Safety and Regulatory Symbols Safety and Regulatory Symbols Safety symbols alert you to potentially dangerous conditions. The symbols apply to specific procedures and appear as needed. Safety Symbols Used for Allegra V-15R Centrifuge Symbol/ Title of Symbol/ Standard Meaning of Symbol from Standard...
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Safety Notice Safety and Regulatory Symbols Safety Symbols Used for Allegra V-15R Centrifuge (Continued) Symbol/ Title of Symbol/ Standard Meaning of Symbol from Standard Regulatory Mark Regulatory Mark Reference Biological Hazard This symbol is used to warn of the ISO 7010 ;...
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ISO 7000, Graphical symbols for use on equipment – Registered symbols b. ISO 7010, Graphical symbols – Registered safety sign Additional Instrument Labels and Symbols The following labels and symbols can also be found on the Allegra V-15R centrifuge. Allegra V-15R Centrifuge Labels Name...
Contents Revision History, iii Safety Notice, v Alerts for Danger, Warning, Caution, and Note, v Safety During Installation and/or Maintenance, vi Instrument Safety Precautions, vi Cleaning, vii Electrical Safety, viii High Voltage, viii Safety Against Risk of Fire, x Mechanical Safety, x Chemical and Biological Safety, xi...
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Control Panel, 1-5 Display Screen, 1-5 Display Fields, Functions Displayed, and Control Panel Buttons, 1-5 Specifications, 1-9 Available Rotors, 1-11 Allegra V-15R Mobile Centrifuge Cart, 1-13 CHAPTER 2: Operation, 2-1 Introduction, 2-1 Installing the Rotor, 2-2 Manual Run, 2-4 Rotor...
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Run, A-5 APPENDIX B: Storage and Transport, B-1 Introduction, B-1 Dimensions and Weight, B-1 Storage Conditions, B-2 Notes On Transport, B-2 Transport Safety Device, B-2 APPENDIX C: Acceleration and Deceleration Profiles, C-1 Introduction, C-1 Description of Allegra V-15R Profiles, C-1 xvii...
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Contents Abbreviations Index Beckman Coulter, Inc. Allegra V-15R Centrifuge Warranty Related Documents xviii...
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2-19 Location of plug for access to door release opening, 3-7 Supplied T-handle Allen wrench (size 5), 3-7 Inserting the emergency door release key, 3-7 Lubricate the drive shaft, 4-3 Allegra V-15R Centrifuge Dimensions (cm/in), A-2 Transport Safety Device, A-3...
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Illustrations Location of locking screws, A-3 Transport Safety Device, B-3 Location of locking screws, B-3...
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Tables Tables • Electrical Plugs and Outlets Suitable for Allegra V-15R , 1-ix • Safety Symbols Used for Allegra V-15R Centrifuge, 1-xii Allegra V-15R Centrifuge Labels, 1-xiv Status Fields and Buttons, 1-6 Specifications, 1-9 Available Rotors for Allegra V-15R, 1-11...
Scope of Manual This manual is designed to familiarize you with the Beckman Coulter Allegra V-15R refrigerated centrifuge, its functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommends that you read this entire manual, especially the...
In addition, other equipment can radiate RF energy to which this device is sensitive. If one suspects interference between this device and other equipment, Beckman Coulter recommends the following actions to correct the interference: •...
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Introduction Electromagnetic Compatibility (EMC) Compliance unshielded intentional radiators are handheld radio transmitters, cordless phones, and cellular phones. • Do not place this device near medical electrical equipment that can be susceptible to malfunctions caused by close-proximity to electromagnetic fields. • This device has been designed and tested to CISPR 11, Class A emission limits. In a domestic environment, this device can cause radio interference, in which case, you need to take measures to mitigate the interference.
CHAPTER 1 System Description Introduction This chapter provides a brief physical and functional description of Beckman Coulter Allegra V-15R refrigerated centrifuges. The operating controls and indicators are also described. Instructions for using the controls and indicators are in CHAPTER 2, Operation.
Figure 1.1 Allegra V-15R Centrifuge When used with the Allegra V-15R rotors designed for use in this centrifuge, applications this centrifuge can perform include: •...
System Description Centrifuge Chassis • A rotor model identification system prevents the installed rotor from running above its maximum rated speed. During acceleration the microprocessor checks that the identified rotor is supported. Speed is limited to the maximum safe speed of the identified rotor. If the system determines that the set speed exceeds the maximum rated speed of the rotor, the system displays an error message and reduces the speed to the maximum allowable speed of the rotor.
System Description Controls and Indicators Temperature Sensing and Control With the power on, the temperature control system is activated when the door is closed and locked. A sensor in the rotor chamber continuously monitors chamber temperature. The micro controller adjusts the chamber temperature to the temperature entered by the user. The temperature can be set between –10 and +40°C.
System Description Controls and Indicators Control Panel The control panel (Figure 1.3) is mounted at an angle on the centrifuge front for easy visibility and access. It is used to enter run parameters via the display screen, and to display run parameters, program information, and user messages.
System Description Controls and Indicators Table 1.1 Status Fields and Buttons SPEED The set speed of the centrifuge is displayed in the upper area of the Speed/RCF field. Either “Speed” (which is rpm) or “RCF” will be displayed. The actual speed value is displayed directly below.
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System Description Controls and Indicators Table 1.1 Status Fields and Buttons (Continued) ACCELERATION This field is used to select and display the acceleration profile being used. The system offers 10 acceleration profiles (profiles 0-9). Additional detail about acceleration rates and profiles can be found in Acceleration and Deceleration Profiles CHAPTER 2,...
System Description Controls and Indicators Table 1.1 Status Fields and Buttons (Continued) SETTINGS LOCK This field is used to indicate when inputs for changing the parameters set for the centrifuge cannot be changed. When a settings lock has been activated, a padlock symbol will be displayed in the field.
Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Table 1.2 Specifications Specification Allegra V-15R Refrigerated Set Speed 100 to 13,500 in 100 rpm increments Set RCF 10 to 20,412 x g in 10 x g increments...
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System Description Specifications Table 1.2 Specifications (Continued) Specification Allegra V-15R Refrigerated Maximum Noise Output (1 m in 56 dBA front of instrument, 1.5 meters above the floor at Instrument Rated Speed) Ambient Temperature Range 5°C to 31°C Humidity Max. allowable relative humidity of air 75% from 5°C up to 31°C...
System Description Available Rotors Available Rotors The following Beckman Coulter rotors can be used in the Allegra V-15R centrifuge. More detailed specifications for each rotor listed in Table 1.3 can be found in the Allegra V-15R Rotors Instructions For Use (PN C63132).
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System Description Available Rotors Table 1.3 Available Rotors for Allegra V-15R (Continued) Number of Tubes Max RCF ( g) Nominal Part at r Rotor Profile Description Capacity Number VF 6.250 5,450 - Max, 4°C operation 4,824 x g 6 x 250 mL...
Allegra V-15R Mobile Centrifuge Cart A mobile cart is available for the Allegra V-15R centrifuge that can be used as a table for the centrifuge. Wheels on the cart can be locked into place to prevent the cart from moving after the cart has been positioned at a desired location.
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System Description Allegra V-15R Mobile Centrifuge Cart 1-14 PN C63130AF...
This section contains centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the Allegra V-15R Rotors IFU (PN C63132) for instructions on preparing the rotor for centrifugation.
Operation Installing the Rotor Installing the Rotor Prepare the rotor for centrifugation as described in the Allegra V-15R Rotors Instructions For Use (PN C63132). NOTE For runs at temperatures cooler than ambient room temperature, refrigerate the rotor beforehand for fast equilibration.
(i.e., reseated) onto the shaft again. This ensures a proper connection between the rotor and the motor shaft. Detailed information on rotor installation can be found in “Chapter 2: Rotor Preparation and Operation” within the Allegra V-15R Rotors Instructions For Use (PN C63132). PN C63130AF...
Operation Manual Run Manual Run To Perform a Manual Run: Turn the power switch on (Figure 1.2). The display and button will illuminate. The centrifuge is now ready for operation. START Press the button to unlock the door of the centrifuge. The door will automatically DOOR lift open.
The selected rotor or rotor/bucket combination will be applied. Automatic Rotor Identification System The Allegra V-15R centrifuge is equipped with an automatic rotor identification system. If the system detects a different rotor with more than one compatible bucket, the bucket with the lowest maximum speed will be pre-selected by the system and the user will have the option of changing the bucket type.
Operation Manual Run Speed The centrifugation speed is shown in the upper-left section of the display (see Figure 2.3). Either enter a run speed up to the maximum speed of the rotor in use, or enter a Relative Centrifugal Force (RCF) value up to the maximum achievable RCF of the rotor.
Operation Manual Run Hold Run During a Hold Run, the centrifuge run will continue until manually stopped. To set the centrifuge for a Hold Run, perform the following steps: Select the field and press the button. The indication ” will appear next to Time ENTER “set...
NOTE At high run speeds, the centrifuge may not be able to achieve some low temperatures. Refer to the Allegra V-15R Rotors Instructions For Use (PN C63132) for details on this for each rotor. Precooling Depending on the substances to be centrifuged, it may be necessary to precool the centrifuge rotor and sample to ensure the sample temperature is maintained during the run.
Operation Manual Run CAUTION Risk of equipment damage. At temperatures below 0°C, stagnant air in the chamber may cause refrigeration components to freeze. Remove your sample promptly after each run. If pre-cooling the chamber while the rotor is not spinning, set temperature to 8-10°C.
Operation Manual Run program will stop under the following conditions: Rapid Temp • The set value is reached. The run will stop, and there will be an audible signal if the Buzzer function has been activated. • The button is pressed. The run will be stopped immediately. STOP A parameter is changed (except for the temperature).
Figure 2.11 ECO Mode timer expired Acceleration and Deceleration Profiles The Allegra V-15R centrifuge uses acceleration and deceleration profiles to protect the gradient and sample-to-gradient interface. Profiles should be selected depending on the type of run you are performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration and deceleration can be used.
Acceleration/Deceleration a stop. The Allegra V-15R offers 10 deceleration (i.e., braking) profiles (profiles 0-9). Deceleration profile 0 provides deceleration without braking. Additional information about the deceleration profiles provided by the Allegra V-15R can be found in APPENDIX C, Acceleration and Deceleration Profiles.
Operation Manual Run Pulse Run A Pulse Run is a run that will last as long as the button is pressed. It is essentially a short run. START Press and hold the button to initiate a Pulse run. START During the pulse run, the centrifuge accelerates with the maximum acceleration until the maximum speed of the rotor is reached.
Operation Manual Run Activating a Temporary Lock: Position the cursor over the “Padlock” symbol in the lower right-hand corner of the display (Figure 2.14). Figure 2.14 “Padlock” symbol indicating an activated settings lock As long as the padlock symbol is displayed, the centrifuge settings cannot be changed. Activating a Permanent Lock: IMPORTANT The cursor must not be in the Time field when activating a Permanent Lock.
Operation Manual Run Navigate to again (if necessary) and press the button to deactivate the “Auto AutoOpen ENTER Open” function. In this case, the AutoOpen symbol disappears but the bar that was underneath the symbol will continue to flash. Buzzer This function is used to enable an acoustic warning signal that sounds at the end of the centrifugation run, and also in the event of an error message.
Operation Programmed Run Programmed Run The instrument’s internal memory can store programs, which can be recalled by selecting the program number. Saved programs are retained in memory even if the centrifuge's power is turned off. Programs can be protected against modification or deletion when the Program Lock feature is enabled.
Operation Programmed Run Loading and Running a Saved Program If necessary, turn the power switch on (Figure 1.2). The display and button will illuminate. The centrifuge is now ready for operation. START Press the button to open the chamber door. The door will automatically lift open. DOOR Install the rotor using the instructions found in the Installing the Rotor...
Operation Rotor Cycles Program Lock When the Program Lock feature is enabled, the “Save Program” function is disabled. In order to activate the program lock: Select the “ ” symbol with the cursor and confirm the selection. The symbol and the bar ProgLock under the symbol start to flash.
START DOOR NOTE The maximum number of cycles for each rotor and bucket used by the Allegra V-15R centrifuge can be found in the Allegra V-15R Rotors Instructions For Use (PN C63132). When the button is pressed, “...
Maintenance. For any problems not covered in this chapter, contact NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories, before requesting service by Beckman Coulter Field Service. Sections in this chapter include: Diagnostic Error Codes Chart •...
Troubleshooting Procedures Diagnostic Error Codes Chart IMPORTANT Error codes and messages can be acknowledged in order to continue centrifuge operation by pressing the DOOR button. Table 3.1 Diagnostic Error Codes and Messages Chart Error Error Type Definition/Response Recommended Action Number 1 - 9 System Error System Error.
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Troubleshooting Procedures Diagnostic Error Codes Chart Table 3.1 Diagnostic Error Codes and Messages Chart (Continued) Error Error Type Definition/Response Recommended Action Number 40 - 43 Temperature Temperature Error. 1. Allow rotor to stop. Error 2. Turn centrifuge power Off. Rotor stops with maximum braking. 3.
Troubleshooting Procedures Other Possible Problems and Solutions Table 3.1 Diagnostic Error Codes and Messages Chart (Continued) Error Error Type Definition/Response Recommended Action Number 70 - 72 Communication Communication Error. 1. Allow rotor to stop. Error 2. To acknowledge error, turn power off and then Rotor coasts to a stop.
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Set Temperature out of range for • Reduce set speed. reached selected rotor and set speed. • Clean the condenser. Refer to the Allegra V-15R Rotors • Pre-cool or pre-warm rotors before running at IFU. low or high temperatures. Condenser (intake vent) is dirty.
Troubleshooting Procedures Retrieving Your Sample in Case of a Power Failure Table 3.2 Troubleshooting Chart (Continued) Problem Problem/Result Recommended Action Excess humidity in chamber. • Enable ECO Mode or reduce the ECO Mode activation timer duration. Condensation build-up between • Wipe out moisture from the chamber and the runs.
Troubleshooting Procedures Retrieving Your Sample in Case of a Power Failure Figure 3.1 Location of plug for access to door release opening 1. Emergency door release opening Insert the supplied 5mm T-handle Allen wrench (Figure 3.2) horizontally into the emergency door release opening as far as it will go.
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Troubleshooting Procedures Retrieving Your Sample in Case of a Power Failure PN C63130AF...
Turn the power off and disconnect the instrument from the main power source and refer such maintenance to service personnel. For the Allegra V-15R, always adhere to the following in regards to instrument care: • Use a mild detergent or other water-soluble, mild cleaning agents with a pH value between 6 and 8 for cleaning the centrifuge and accessories.
Centrifuge Maintenance Instrument Care Centrifuge Maintenance WARNING Risk of personal injury. The gas dampers provide support for the centrifuge door. Regularly check that the centrifuge door remains in its fully opened position until it is manually closed. Worn gas dampers will cause the door to fall. Gas dampers must be replaced immediately when they are no longer are able to hold the door in its fully opened position.
Centrifuge Maintenance Instrument Care WARNING Risk of personal injury or contamination. Consult with your Lab Safety Officer or laboratory guidelines if there is a risk of toxic, radioactive, or pathogenic contamination. Always wear suitable PPE. • If the centrifuge has been contaminated with toxic, radioactive, or pathogenic substances, clean the rotor chamber immediately with a suitable decontamination agent.
Risk of equipment damage. Plastic parts can be damaged if solvents, acids, or alkaline solutions are used on the plastic surfaces of the Allegra V-15R centrifuge. Remove the rotor from the centrifuge and clean the drive shaft, shaft cavity, threads, and the tie-down screw regularly using a mild detergent such as Solution 555 and a soft brush.
Centrifuge Maintenance Instrument Care Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555. Dilute the detergent with water (10 parts water to 1 part detergent). IMPORTANT Do not use acetone. Glass Tube Breakage WARNING Risk of personal injury. If a glass tube breaks, glass fragments may escape the bucket or rotor.
Chemical Resistances (publication IN-175) for more information regarding chemical resistance of centrifuge and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.
Circuit Breaker and Fuses Circuit Breaker and Fuses There are no user-replaceable fuses in the Allegra V-15R centrifuge. If the centrifuge circuit breaker trips for any reason, the power switch will move to the off ( position. Reset the circuit breaker by turning the power switch back to the on ( ) position.
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Centrifuge Maintenance Supply List PN C63130AF...
• Locate the centrifuge in an area with sufficient ventilation to allow for sufficient heat dissipation. • Position the centrifuge on a level surface, such as the Allegra V-15R Mobile Centrifuge Cart (see Supply List), a sturdy table, or a laboratory bench that is able to support the weight of the...
• Always lift the centrifuge with the assistance of additional persons. • When lifting the centrifuge, always reach under the centrifuge from the side. • Position the centrifuge on a level surface, such as the Allegra V-15R Mobile Centrifuge Cart (see Supply List...
Removing Transport Safety Device IMPORTANT The Transport Safety Device must be removed before operating the Allegra V-15R Centrifuge. The Transport Safety Device consists of two Allen screws that secure the centrifuge's motor in place for shipping purposes. These two screws must be removed before the centrifuge can be used.
• Do not place containers holding liquid on or near the chamber door. If spilled, liquid may get into the centrifuge and damage electrical components. • The power line cord, for the Allegra V-15R, is the disconnecting device used to remove electrical power. Make sure there is adequate clearance around the centrifuge in order to reach the line cord.
Unpacking and Installation Test Run Table A.1 Electrical Plugs and Outlets Suitable for Allegra V-15R (Continued) Part Number Instrument Rating Suitable Cord Plug Suitable Cord Outlet C63161, C63190 220-240 VAC, 50 Hz, 9.5A C63186, C63187 220 VAC, 60 Hz, 10.3A Additional electrical specifications can be found in Specifications.
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Unpacking and Installation Test Run PN C63130AF...
APPENDIX B Storage and Transport Introduction This Appendix provides storage requirements for the Allegra V-15R centrifuge, and information on preparing the centrifuge for shipping. Sections in this chapter include: Dimensions and Weight • Storage Conditions • Notes On Transport •...
WARNING Risk of personal injury or equipment damage. The Allegra V-15R centrifuge weighs 110 kg (243 lb). Do not attempt to lift or move it without assistance. Follow your safety officer’s instructions regarding lifting heavy objects.
Storage and Transport Transport Safety Device Installation Lift the centrifuge from the front of unit and tilt it backward to expose the bottom of the unit. Stabilize the centrifuge by placing a suitable object, such as a wooden block, under the centrifuge.
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Storage and Transport Transport Safety Device PN C63130AF...
Allegra V-15R centrifuge. Description of Allegra V-15R Profiles The acceleration profiles produced by the Allegra V-15R are numbered 0 - 9 to reflect increasing acceleration rate with 9 being the maximum. The deceleration profiles are also numbered 0 - 9 to reflect increasing deceleration rate.
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Acceleration and Deceleration Profiles Description of Allegra V-15R Profiles Table C.1 Allegra V-15R Acceleration and Deceleration Profiles (Continued) Acceleration Deceleration Time to 1000 Slope above 1000 RPM Time to 1000 RPM Slope above 1000 RPM Profile RPM (seconds) (rpm / sec)
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Abbreviations RCF — Relative Centrifugal Force A — Ampere Rmax — Maximum radius Btu — British thermal unit RPM — Rotations per minute bps — Bits per second SDS — Safety data sheets °C — Degrees Celsius or Degrees Centigrade V —...
Subject to the exceptions and upon the conditions specified below and the warranty clause of the Beckman Coulter, Inc. terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop...
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