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Summary of Contents for Sartorius Tacta
- Page 1 Operating Instructions Original Operating Instructions Tacta ® Mechanical Pipette Single-channel Models | Multi-channel Models 1000134913...
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Page 3: Table Of Contents
Repetitive Reverse Pipetting ..........21 Operating Instructions Tacta... - Page 4 13 Sartorius Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Operating Instructions Tacta...
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Page 5: About These Instructions
30 – 300 LH-729140 12-channel 1 – 10 LH-729220 10 – 100 LH-729230 30 – 300 LH-729240 Related Documents In addition to these instructions, observe the following documents: Instructions for consumables, accessories, e.g., pipette holder, pipette tips Operating Instructions Tacta ®... -
Page 6: Target Groups
Denotes a hazard that may result in property damage if it is not avoided. 1.4.2 Other Symbols Used Required action: Describes activities that must be carried out. The activities in the sequence must be carried out in succes- sion. Result: Describes the result of the activities carried out. Operating Instructions Tacta ®... -
Page 7: Safety Instructions
Device-specific documents and product approvals may lose their validity. Sartorius recommends that any repair work, even that carried out after the end of the warranty period, is carried out by Sartorius Service or after con- sulting with Sartorius Service. Operating Instructions Tacta... -
Page 8: Qualifications Of Personnel
Take regular breaks during pipetting and in doing so relax the hand. Stand or sit upright during pipetting. Place the index finger under the finger support and operate the device with the thumb. Do not cramp during pipetting. Operating Instructions Tacta ®... -
Page 9: Device Description
Operating button Finger support Tip ejector button Handle Volume lock button Display Tip ejector collar Tip cone Safe-Cone filter Adjustment socket and settings window for adjustment Tip cone housing Tip ejector bar Stainless steel, corrosion-resistant metal ring Operating Instructions Tacta ®... -
Page 10: Optiload Tip Cones And Optiject Ejection System
Sartorius tips are made of pure virgin polypropylene and manufactured in protected cleanroom conditions. Sartorius non-filter tips are available in trays, bulk packages, and space-saving refill systems. Both non-filter tips and trays are autoclavable at 121°C (252°F), 20 min, 1 bar (15 psi). All Single Tray racks and clean Refill Packs are certified RNase, DNase, and endotoxin-free. -
Page 11: Adjustment Tool
Follow-up adjustment: The pipette can be re-adjusted, e.g., if it is determined that the delivered volume is not within the error limits or a liquid other than water is to be pipetted. The described procedure must be used for the adjustment. The current pipette adjustment is displayed in the settings window for adjustment. Operating Instructions Tacta ®... -
Page 12: Installation
Quick Start Guide Unpacking Procedure Unpack the device. Sartorius recommends keeping the original packaging to return the de- vice appropriately, e.g., for repairs. Tagging the Pipette Each pipette can be identified with a tag on the finger support. Use person- alized tags to make each pipette easily identifiable. -
Page 13: Storing The Pipette When Not In Use
Allow a waiting time of 20 minutes. This allows the adhesive tape and surface to bond. Hook the finger support onto the holder. Using the Carousel Stand Adapter The carousel stand is made compatible with Tacta by using the adapter ® provided in the carousel stand package (carousel stand and adapter see Chapter “12.1 Accessories”, page 46). -
Page 14: Commissioning
Ensure that you do not turn the volume setting beyond the specified range. Procedure Press and slide the volume lock button. Turn the operating button to adjust the volume. Release the button to lock the volume adjustment again. Operating Instructions Tacta ®... -
Page 15: Option 2: Setting The Volume With Volume Lock Disengaged
Slide the volume lock button up. The red bar becomes visible and vol- ume adjustment is now unlocked. Turn the operating button to set the volume. Slide the volume lock button back down. The volume adjustment is now locked, preventing accidental volume changes. Operating Instructions Tacta ®... -
Page 16: Attaching A Tip
Commissioning Attaching a Tip Sartorius recommends using suitable pipette tips for maximum accuracy and precision (see Chapter „12.2 Consumables“, page 46). The Optiload spring-loaded tip cone of the pipette ensures optimal sealing. Fig. 4: Attaching a tip with single- and multi-channel model (example) Procedure Make sure the pipette tip cone(s) are clean and undamaged. -
Page 17: Ejecting A Tip
Do not force the pipette into the tip, or rock it from side to side, or front to back when attaching tips. Ejecting a Tip The pipette has the Optiject ejection system for smooth tip ejection. Procedure Hold the pipette over a suitable waste vessel. Press the tip ejector with your thumb. Operating Instructions Tacta ®... -
Page 18: Inserting Or Ejecting A Safe-Cone Filter
Commissioning Inserting or Ejecting a Safe-Cone Filter Replaceable Sartorius Safe-Cone Filters can be used with your pipette to help prevent liquids and aerosols from entering the pipette housing, and causing contamination or damage. We recommend to use Safe-Cone filters when using non-filter tips (except for 3 and 10 μl models). -
Page 19: Operation
— When handling infectious or radioactive agents, wear appropriate pro- tective gear and take all sensible precautions. — Avoid exposing the unit to extreme temperature changes, humidity, or dust. Observe the operating conditions (see Chapter “11.2 Ambient Con- ditions at the Installation Site”, page 37). Operating Instructions Tacta ®... -
Page 20: Forward Pipetting
Place the tip just under the surface of the liquid and smoothly release the operating button, allowing it to return to the starting position. Carefully withdraw the tip from the liquid, touching it against the edge of the container to remove any excess from the tip. Operating Instructions Tacta ®... -
Page 21: Repetitive Reverse Pipetting
Press the operating button smoothly to the first stop (1) to deliver the preferred volume. The liquid that remains in the tip may not be in- cluded in the delivery. When finished dispensing: Discard the remaining liquid by pressing the operating button to the second stop (2). Operating Instructions Tacta ®... -
Page 22: Cleaning And Maintenance
(see Chapter “11.3 Materials”, page 38 and Chapter “11.4 Cleaning Procedure and Cleaning Agent”, page 40). We recommend using gloves when cleaning the pipette. Procedure Daily check the Tacta pipette to ensure it is clean. ® Gently clean the surface of the pipette with the moistened cloth and wipe it dry. -
Page 23: Maintenance Schedule
7.4, 24 models: Lower components. part of the device Multi-channel For cleaning and greasing the models: Lower lower parts contact Sartorius Ser- part of the device vice. Device Sterilise the device. 7.5, 28 Regularly, e.g., every 3 months and af-... -
Page 24: Cleaning And Inspecting The Pipette
Disassembling the Lower Part of the Pipette (Single-channel Pipettes) The following information only applies to single-channel pipettes. For clean- ing multi-channel pipettes contact Sartorius Service. Pipette Models (Single-channel Models) 3 | 10 | 20 | 100 μl 200 | 1,000 μl 5,000 μl | 10,000 μl... - Page 25 Do not turn the locking ring and the tip cone si- multaneously as this will break the pipette. For 10,000 μl volume models: Hold the tip cone cylinder firmly and turn the locking ring clockwise. Do not turn the tip cone as this will break the pipette. Operating Instructions Tacta ®...
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Page 26: Cleaning And Greasing The Lower Part Of The Pipette
Cleaning and Greasing the Lower Part of the Pipette (Single-channel Pipettes) Avoid excess grease, too much grease can block the piston. Only use grease that is provided or recommended by Sartorius. The following information only applies to single-channel pipettes. For clean- ing multi-channel pipettes contact Sartorius Service. -
Page 27: Assembling The Lower Part Of The Pipette (Single-Channel Pipettes)
Before reassembling, check that there is no lint or other particles on the sur- face of the piston. The following information only applies to single-channel pipettes. For clean- ing multi-channel pipettes contact Sartorius Service. Procedure Depending on the pipette volume reassemble the lower parts as follows: For 3 μl to 1,000 μl volume models: Carefully place the tip cone cylin-... -
Page 28: Sterilizing The Pipette
Sterilizing the Pipette Using UV Radiation Tacta pipettes are made of UV-resistant materials and tolerate temporary ® exposure to UV radiation. Take note that prolonged or frequent exposure to UV radiation may cause yellowing and brittling of the pipette. Operating Instructions Tacta ®... -
Page 29: Defining The Test Routine For The Routine Test
The test liquid from the other channels is discarded as residual water. Procedure Prepare a test routine that takes into account the required criteria for the routine test on the device. Operating Instructions Tacta ®... -
Page 30: Performing The Routine Test
Hold the device vertically, press the operating button and immerse the tip below the surface of the water to the specified immersion depth. Slowly release the operating button. Comply with the specified wait time. Remove the device from the test water container vertically. Operating Instructions Tacta ®... -
Page 31: Dispensing Test Water And Performing The Measurement
For the conformity assessment, calculate the random measurement de- viation as standard deviation or as variation coefficient: — As standard deviation (n = number of measurements (10)) — As variation coefficient C = 100% sr / V Operating Instructions Tacta ®... -
Page 32: Adjusting The Pipette
If the pipette has been adjusted for the use of liquids other than water, attach a label to the outside of the pipette with the name of the liquid and the adjusted volume range. To do this, the supplied tags can be used for labeling. Operating Instructions Tacta ®... -
Page 33: Malfunctions
Foreign particles between the tip and Clean tip cone, attach new tip cone Tip cone holder incorrectly tightened Tighten the tip cone holder Pipette damaged Send to Sartorius Service for mainte- nance Pipette outside established Incorrect operation Follow instructions performance specifications... -
Page 34: Storage And Shipping
Transport damage as well as measures for subsequent cleaning and disin- fection of the device or parts by Sartorius are charged to the sender. Devices contaminated with hazardous materials, e.g., harmful biological or chemical substances, will not be accepted for repair or disposal. -
Page 35: 10 Disposal
The device is decontaminated. Procedure Dispose of the device in accordance with local government regulations. Dispose of the packaging in accordance with local government regula- tions. Dispose of the consumables in accordance with local government regu- lations. Operating Instructions Tacta ®... -
Page 36: Technical Data
Technical Data Technical Data 11.1 Dimensions and Weights 11.1.1 Single-channel Model Unit Value Value Value Value Value Value Value Value Dimensions (length) Weight 11.1.2 Multi-channel Model Unit Value Value Value Value Value Value Dimensions (length) Weight Operating Instructions Tacta ®... -
Page 37: Ambient Conditions At The Installation Site
+15 – +30 During performance review °C +15 – +30 In storage | during transport °C -20 – +40 Relative humidity, in operation At temperatures up to 31°C, maximum No potentially explosive areas Other properties Store dry Operating Instructions Tacta ®... -
Page 38: Materials
1.4404 / AISI 316L Volume lock button Display (single-channel models) Settings window for ad- justment Lower part of housing Tip cone housing (multi-channel models) Safe-Cone filter Tip ejector bar (multi-channel models) Tip ejector collar (sin- gle-channel models) Operating Instructions Tacta ®... -
Page 39: Model-Dependant Materials Of Lower Part Of Housing (Lower Part)
For 100 μl multi-channel model For 200 μl | 300 μl mod- For 1,000 μl | 5,000 μl | 10,000 μl models Piston seal For 3 µl | 10 µl | 20 µl | 10,000 µl models For 100 μl | 200 μl | 300 μl | 1,000 μl | 5,000 μl models Operating Instructions Tacta ®... -
Page 40: Cleaning Procedure And Cleaning Agent
Wiping the device surfaces with a lightly moistened cloth Wiping the device surfaces dry Autoclaving UV radiation Autoclaving Autoclaving temperature, maximum °C Permissible overpressure Duration, maximum Approved cleaning agents Ethanol (70%) Isopropyl alcohol (60%) Mild cleaning agent, suitable for the device materials Operating Instructions Tacta ®... -
Page 41: Adjustment
10 µl, 8 channels | 12 channels 0.01 -0.45 -0.1 -0.05 -0.01 0.01 0.05 0.45 100 µl, 8 channels | 12 channels -4.5 -0.5 -0.1 300 µl, 8 channels | 12 channels -0.2 * Factory settings, setting for pipetting water Operating Instructions Tacta ®... -
Page 42: Routine Test
1.0035 1.0035 1.0036 23.5 1.0036 1.0036 1.0036 1.0037 The values in the table represent the most common values. For the full table, or for formula to calculate correction factor the standard ISO 8655-6 must be considered. Operating Instructions Tacta ®... -
Page 43: Performance Specifications
* The listed values for the systematic and random errors are valid under the following conditions: — Use of the listed models with suitable Sartorius Optifit non-sterile tips — Determination of the values under strictly controlled conditions during type examinations in accordance with... - Page 44 * The listed values for the systematic and random errors are valid under the following conditions: — Use of the listed models with suitable Sartorius Optifit non-sterile tips — Determination of the values under strictly controlled conditions during type examinations in accordance with...
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Page 45: Multi-Channel Models
* The listed values for the systematic and random errors are valid under the following conditions: — Use of the listed models with suitable Sartorius Optifit non-sterile tips — Determination of the values under strictly controlled conditions during type examinations in accordance with... -
Page 46: 12 Accessories And Consumables
Extended Filter Tips 10–1,000 µL, single rack LH-XF781001 Safe-Cone Filter, standard, ∅6.73 mm 721005 Safe-Cone Filter, standard, ∅5.33 mm 721006 Safe-Cone Filter, standard, ∅3.15 mm 721007 Safe-Cone Filter, standard, ∅2.51 mm 721008 Safe-Cone Filter, standard, ∅1.83 mm 721014 Tweezers for filter replacement 721009 Operating Instructions Tacta ®... -
Page 47: 13 Sartorius Service
For inquiries about the device or when contacting Sartorius Service in the event of a malfunction, ensure that you have the device information, e.g., serial number, close at hand so you can pass it on to Sartorius Service. To do this, refer to the information on the manufacturer’s ID label. - Page 48 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.
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