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Summary of Contents for Sartorius Proline Plus
- Page 1 Operating Instructions Original Operating Instructions Proline Plus ® Mechanical Pipette Single-channel Models | Multi-channel Models 1000134949...
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Page 3: Table Of Contents
Contents Contents 1 About These Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Validity . - Page 4 13 Sartorius Service . . . . . . . . . . . . . . . . . . .
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Page 5: About These Instructions
About These Instructions About These Instructions Validity These instructions are part of the device; they must be read in full and kept in a safe place. These instructions apply to the device in the following versions: Device Volume Range (µl) Maximum Volume / Item number Nominal Volume (µl) -
Page 6: Related Documents
About These Instructions Device Volume Range (µl) Maximum Volume / Item number Nominal Volume (µl) Single-channel 1000 1000 728570 2000 2000 728575 5000 5000 728580 10000 10000 728590 Related Documents In addition to these instructions, observe the following documents: In- structions for consumables, accessories, e.g., pipette holder, pipette tips Target Groups These instructions are addressed to the following target groups. -
Page 7: Safety Instructions
Device-specific documents and product approvals may lose their validity. Sartorius recommends that any repair work, even that carried out after the end of the warranty period, is carried out by Sartorius Service or after con- sulting with Sartorius Service. Operating Instructions Proline Plus ®... -
Page 8: Qualifications Of Personnel
Safety Instructions Qualifications of Personnel Persons who do not possess adequate knowledge about how to use the de- vice may injure themselves and other persons. If a particular qualification is required for an activity: The target group is specified. If no qualification is specified: The activity can be performed by the “Operator”... -
Page 9: Device Description
Device Description Device Description Device Overview Fig. 1: Multichannel and single-channel models (example) Pos . Name Operating button Finger support Tip ejector button Handle Calibration screw cover Tip ejector collar Tip cone Display Tip cone housing Tip ejector bar Operating Instructions Proline Plus ®... -
Page 10: Pipette Tips
Sartorius tips are made of pure virgin polypropylene and manufactured in protected cleanroom conditions. Sartorius non-filter tips are available in trays, bulk packages, and space-saving refill systems. Both non-filter tips and trays are autoclavable at 121°C (252°F), 20 min, 1 bar (15 psi). All Single Tray racks and clean Refill Packs are certified RNase, DNase, and endotoxin-free. -
Page 11: Adjustment Tool
Device Description Adjustment Tool The adjustment tool is required to adjust the pipette and perform assembly work on the pipette. Fig. 3: Adjustment tool Pos . Name For calibration For opening the cover of the calibration screw Pipetting Practice The Proline Plus pipette is used for forward and reverse pipetting. -
Page 12: Installation
Unpacking Procedure Unpack the device. Sartorius recommends keeping the original packaging to return the de- vice appropriately, e.g., for repairs. Storing the Pipette When Not in Use When not in use, the pipette must always be stored upright for convenient use and safety reasons. -
Page 13: Commissioning
Commissioning Commissioning Adjusting the Volume (Only Pipettes with Variable Volumes) The volume to be aspirated (pipetting volume) is shown on the display. To adjust the volume rotate the operating button. NOTICE Damage to the setting mechanism! The setting mechanism can become damaged if turned too far. Ensure that you do not turn the volume setting beyond the specified range. -
Page 14: Ejecting The Tip
Press the tip ejector button with your thumb. Inserting or Removing a Safe-Cone Filter Replaceable Sartorius Safe-Cone Filters can be used with your pipette to help prevent liquids and aerosols from entering the pipette housing, and causing contamination or damage. -
Page 15: Inserting A Safe-Cone Filter
Commissioning 5.4.1 Inserting a Safe-Cone Filter Procedure Clean the tip cone if necessary. Insert a new filter. 5.4.2 Removing a Safe-Cone Filter Procedure Remove the Safe-Cone Filter with the provided tweezer. Operating Instructions Proline Plus ®... -
Page 16: Operation
Operation Operation Considering Good Pipetting Practices — Make sure the tip is firmly attached to the tip cone(s). — Pre-rinse the tip before aspirating by filling and emptying the tip three to five times. This is particularly important when pipetting liquids with a vis- cosity and density greater than water, or volatile liquids with high vapor pressure (such as ethanol). -
Page 17: Forward Pipetting
Operation Forward Pipetting Forward pipetting is the most common pipetting technique. It uses a blow- out function to ensure complete delivery of the liquid. This technique aspi- rates and then dispenses the selected liquid volume. It is recommended for aqueous liquids, liquids containing small amounts of detergents or proteins, and solvents. -
Page 18: Repetitive Reverse Pipetting
Operation Press the operating button smoothly to the first stop (1) to deliver the preferred volume. The liquid that remains in the tip may not be included in the delivery. Discard the remaining liquid by pressing the operating button to the sec- ond stop (2). -
Page 19: Cleaning And Maintenance
Cleaning and Maintenance Cleaning and Maintenance Testing Chemical Resistance Information on interactions of pipette materials with organic and inorganic solutions, solvents and corrosive chemicals can be provided on request. Procedure Check that the pipette materials are chemically compatible with the de- tergent, disinfectant, or decontaminant liquids to be used (pipette mate- rials see Chapter “11.3 Materials”, page 35). -
Page 20: Maintenance Schedule
7.4, 21 models: Lower components. part of the device Multi-channel For cleaning and greasing the models: Lower lower parts contact Sartorius Ser- part of the device vice. Device Sterilise the device. 7.5, 25 Regularly, e.g., every 3 months and Device Perform a routine test to check 7.7, 27... -
Page 21: Cleaning And Inspecting The Pipette
Disassembling the Lower Part of the Pipette (Single-channel Pipettes) The following information only applies to single-channel pipettes. For clean- ing multi-channel pipettes contact Sartorius Service. Pipette Models (Single-Channel Models) 5 | 10 | 20 | 25 | 50 | 100 μl 200 | 250 | 500 | 1000 μl... - Page 22 Cleaning and Maintenance Procedure If available: Remove the tip cone filter (see Chapter “5.4 Inserting or Re- moving a Safe-Cone Filter”, page 14). Unscrew the tip ejector collar counterclockwise and remove it. Depending on the pipette volume disassemble the lower parts of the pi- pette as follows: For 3 μl to 1,000 μl volume models: Unscrew the locking ring counter- clockwise and carefully remove it together with the tip cone.
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Page 23: Cleaning And Greasing The Lower Part Of The Pipette
Cleaning and Greasing the Lower Part of the Pipette (Single-channel Pipettes) Avoid excess grease, too much grease can block the piston. Only use grease that is provided or recommended by Sartorius. The following information only applies to single-channel pipettes. For clean- ing multi-channel pipettes contact Sartorius Service. -
Page 24: Assembling The Lower Part Of The Pipette
Before reassembling, check that there is no lint or other particles on the surface of the piston. The following information only applies to single-channel pipettes. For cleaning multi-channel pipettes contact Sartorius Service. Procedure Depending on the pipette volume reassemble the lower parts as follows: For 3 μl to 1,000 μl volume models: Carefully place the tip cone cylin-... -
Page 25: Sterilizing The Pipette
Cleaning and Maintenance Sterilizing the Pipette The Proline Plus pipette can be sterilized using autoclaving, UV radiation, ® disinfectant, or decontaminant liquids. Always follow the instructions below. 7.5.1 Autoclaving the Pipette Procedure If available: Eject the tip cone filter (see Chapter “5.4 Inserting or Re- moving a Safe-Cone Filter”, page 14). -
Page 26: Defining The Test Routine For The Routine Test
Maximum permitted error limits for sys- As per ISO 8655-2 tematic and random errors, as per ISO 8655-2 Acceptable error range taking into ac- Sartorius specifications were count: achieved in strictly controlled con- — Application ditions, as per ISO 8655-6 (see —... -
Page 27: Performing The Routine Test
Cleaning and Maintenance Performing the Routine Test A routine test with an analytical balance (single-channel balance) is de- scribed below. Further information on the possible test routines is described in the ISO 8655 series of standards, e.g., for the testing of multi dispensing in pipetting mode. -
Page 28: Dispensing Test Water And Performing The Measurement
Cleaning and Maintenance 7.7.2 Dispensing Test Water and Performing the Measure- ment We recommend performing 10 measurements. The tip must be replaced after 5 measurements. Requirements The analytical balance is ready for measuring. Procedure Dispense the test water. To do this, proceed as follows: Hold the tip above the liquid level at an angle of 30° – 40°... -
Page 29: Adjusting The Pipette
Cleaning and Maintenance Compare the systematic error and the random error with the values of the performance specification from your own laboratory. If the measurement results are within the performance specification: The device is ready for use. If the measurement results are not within the performance specification: Check the device for both systematic errors and random errors. -
Page 30: Malfunctions
Foreign particles between the tip and Clean tip cone, attach new tip cone Tip cone holder incorrectly tightened Tighten the tip cone holder Pipette damaged Send to Sartorius Service for mainte- nance Pipette outside established Incorrect operation Follow instructions performance specifications... -
Page 31: Storage And Shipping
Transport damage as well as measures for subsequent cleaning and disin- fection of the device or parts by Sartorius are charged to the sender. Devices contaminated with hazardous materials, e.g., harmful biological or chemical substances, will not be accepted for repair or disposal. -
Page 32: 10 Disposal
Disposal 10 Disposal 10.1 Disposing of the Device and Parts The device and the device accessories must be disposed of properly by dis- posal facilities. Requirements The device is decontaminated. Procedure Dispose of the device in accordance with local government regulations. Dispose of the packaging in accordance with local government regula- tions. -
Page 33: Technical Data
Technical Data Technical Data 11.1 Dimensions and Weights 11.1.1 Single-Channel Models with Variable Volume Unit Value Value Value Value Value Value Value Value Value Dimensions (length) Weight 11.1.2 Multichannel Models with Variable Volume Unit Value Value Value Value Value Value Dimensions (length) Weight 11.1.3... -
Page 34: Ambient Conditions At The Installation Site
Technical Data Models with Volumes of 500 µl to 10,000 μl Unit Value Value Value Value Value Dimensions (length) Weight 11.2 Ambient Conditions at the Installation Site Unit Value Laboratory, for indoor use only Pollution level according to IEC 61010-1 Maximal height above sea level 2000 Suitable for protection class Protection class of the device, as per DIN EN 60529-1... -
Page 35: Materials
Technical Data 11.3 Materials 11.3.1 Upper Part and Lower Part of Housing Component Materials Top section of the housing Operating button Finger support Tip ejector button Handle Calibration screw cover Display Bottom section of the housing Tip ejector collar Tip cone housing (multi- channel models) Safe-Cone Filter Tip ejector bar (multi-... -
Page 36: Model-Dependant Materials Of Lower Part Of Housing (Lower Part) And Tip Ejector Components
Technical Data 11.3.2 Model-Dependant Materials of Lower Part of Housing (lower part) and Tip Ejector Components Component Materials Locking ring Only for 10,000 μl model All other models Tip cone cylinder (sin- gle-channel models) 3 | 5 | 10 | 20 | 25 | 50 | 100 μl models 200 | 250 | 500 | 1000 | 2000 | 5000 | 10,000 μl... -
Page 37: Cleaning Procedure And Cleaning Agent
Technical Data 11.4 Cleaning Procedure and Cleaning Agent Unit Value Approved cleaning procedures Wiping the device surfaces with a lightly moistened cloth Wiping the device surfaces dry Autoclaving UV radiation Autoclaving Autoclaving temperature, maximum °C Permissible overpressure Duration, maximum Approved cleaning agents Ethanol (70%) Isopropyl alcohol (60%) Mild cleaning agent, suitable for the device materials... -
Page 38: Adjustment
Technical Data 11.5 Adjustment 11.5.1 Initial Adjustment Type of adjustment by manufacturer in accordance with ISO 8655-1 On dispensing (Ex) of the selected volume (test volume), at 20 °C 11.6 Routine test 11.6.1 Conditions for Aspirating the Test Liquid Test volume (µL) Immersion depth of the pipette tip Wait time during aspiration (mm) -
Page 39: Performance Specifications
* The values specified for the systematic and random error limits apply under the following conditions: — When using the specified models with suitable non-sterile Sartorius Optifit tips — When calculating the values under strictly controlled conditions during type examinations in accordance with... - Page 40 * The values specified for the systematic and random error limits apply under the following conditions: — When using the specified models with suitable non-sterile Sartorius Optifit tips — When calculating the values under strictly controlled conditions during type examinations in accordance with...
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Page 41: Multichannel Models, With Variable Volume
* The values specified for the systematic and random error limits apply under the following conditions: — When using the specified models with suitable non-sterile Sartorius Optifit tips — When calculating the values under strictly controlled conditions during type examinations in accordance with... -
Page 42: Single-Channel Models, With Fixed Volume
* The values specified for the systematic and random error limits apply under the following conditions: — When using the specified models with suitable non-sterile Sartorius Optifit tips — When calculating the values under strictly controlled conditions during type examinations in accordance with... -
Page 43: Accessories And Consumables
Accessories and Consumables 12 Accessories and Consumables 12.1 Accessories This table contains a selection of the accessories that can be ordered. For information on other products, contact Sartorius. Item Quantity Order number Pipette holder LH-727640 Carousel stand for 6 pipettes... -
Page 44: 13 Sartorius Service
For inquiries about the device or when contacting Sartorius Service in the event of a malfunction, ensure that you have the device information, e.g., serial number, close at hand so you can pass it on to Sartorius Service. To do this, refer to the information on the manufacturer’s ID label. - Page 45 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.
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