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Summary of Contents for Sartorius Vivaspin Turbo 15 RC
- Page 1 Instructions for Use Vivaspin Turbo 15 RC ® Centrifugal Ultrafiltration Devices for Research Use 2563320-000-02...
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Page 3: Table Of Contents
Contents Contents 1 About these Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Scope . - Page 4 Contents 8 Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 8.1 Dimensions .
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Page 5: About These Instructions
About these Instructions About these Instructions Scope These instructions are part of the product. These instructions apply to the following versions of the product: Vivaspin Turbo 15 RC Quantity Prod . no . ® 5 kDa 12 | 48 VS15TR11 | VS15TR12 10 kDa 12 | 48 VS15TR01 | VS15TR02... -
Page 6: Symbols Used
About these Instructions Symbols Used 1.3.1 Warnings in Operation Descriptions NOTICE Denotes a hazard that may result in property damage if it is not avoided. 1.3.2 Other Symbols Required action: Describes actions that must be carried out. The actions in the sequence must be carried out in succession. Result: Describes the result of the actions carried out. -
Page 7: Safety Instructions
Safety Instructions Safety Instructions General Functions The product is intended for the ultrafiltration and | or diafiltration of biological and aqueous solutions. The sample solutions and volumes used must be suit- able for the product. The filtration process must be carried out in a centrifuge. Macromolecules that are sufficiently larger than the nominal pore size of the membrane are re- tained above the membrane and progressively concentrated. -
Page 8: Significance Of These Instructions
Safety Instructions Significance of these Instructions Failure to follow the instructions might have serious consequences, e.g. dan- ger to individuals. Read the instructions carefully and completely. The instructions for action build on each other. Ensure that the information contained in these instructions is available to all individuals working with the product. -
Page 9: Product Description
Product Description Product Description Product Overview Fig. 1: Product overview (example) Pos . Description Concentrator cap Concentrator Twin vertical membranes Filtrate container Angular dead stop pocket Instructions for Use Vivaspin Turbo 15 RC ®... -
Page 10: Product Symbols
Product Description Product Symbols Symbol Description Catalogue number Batch code Use by Non-sterile product Only suitable for research purposes Do not re-use Consult instructions for use Temperature limits Instructions for Use Vivaspin Turbo 15 RC ®... -
Page 11: Process Preparation
Process Preparation Process Preparation Scope of Delivery Article Quantity Product, packed in a cardboard box 12 or 48 Instructions for Use Unpacking Procedure NOTICE Risk of product malfunctions due to exceeding the usability! Check the usability of the product (see specification on packaging). Dispose of products for which the usability has been exceeded. -
Page 12: Operation
Operation Operation Pre-Rinsing the Product Membranes in the product may contain traces of glycerin. If this substance can interfere with the analysis of the sample: The membranes may be rinsed before filtration. Procedure Remove the concentrator cap. Use a pipette to apply a filling volume of buffer solution or deionized water into the concentrator. -
Page 13: Performing Filtration
Please ensure that the molecular weight cut-off (MWCO) of the product is suitable for the size of the target molecule to be concentrated. In order to ensure maximum recovery of the target molecule, Sartorius recommends selecting a MWCO that is at least 50% below the size of the target molecule. -
Page 14: Inserting The Product Into The Centrifuge
Operation 5.3.2 Inserting the Product into the Centrifuge Procedure NOTICE Risk of product malfunctions or dam- age to the centrifuge! Only use the product in suitable centrifuges (see Chapter “8.5.2 Cen- trifuges”, page 20). Insert the product into the centrifuge. If a centrifuge with fixed-angle rotor is used: Place the product into the centrifuge as follows: −... -
Page 15: Removing The Sample
Operation 5.3.4 Removing the Sample Procedure If the filtration or concentration is complete: Remove the product from the centrifuge. Remove the concentrator cap. Recover the sample from dead stop pocket of the concentrator using a pipette. If the membrane was pre-rinsed before filtration: Decant the filtrate and concentrate. -
Page 16: Storage
Storage Storage Storing the Product If the product has been unpacked and the membranes have been pre-rinsed: The membranes must be protected against drying out. For this purpose, the membranes must be stored in a moist and cool condition. NOTICE Risk of damage to the product due to improper storage! Comply with the storage specifications. -
Page 17: Disposal
Disposal Disposal Decontaminating the Product If the product has come into contact with hazardous substances: Steps must be taken to ensure proper decontamination and declaration. The operator of the product is responsible for adhering to local government regulations on the proper decontamination and declaration for transport and disposal. Procedure If the product has come into contact with hazardous substances: Decontaminate the product. -
Page 18: Technical Specifications
Technical Specifications Technical Specifications Dimensions Unit Value Length x Diameter 118 x 30 Active membrane surface Weight Materials Materials Concentrator Styrene butadiene copolymer (SBC) Concentrator cap | Filtrate container Polypropylene Membranes Regenerated cellulose Instructions for Use Vivaspin Turbo 15 RC ®... -
Page 19: Ambient Conditions
Technical Specifications Ambient Conditions Unit Value Storage temperature When packed °C +4 – +40 When unpacked, with membrane kept moist °C +2 – +8 Operating Conditions Centrifuge Centrifuge with with swing fixed-angle bucket rotor rotor (25°) Unit Value Value For use with centrifuge Filling volume, minimum Filling volume, maximum Membrane hold-up... -
Page 20: Equipment Required
Technical Specifications Equipment Required 8.5.1 Pipettes Pasteur pipette, variable volume or fixed volume pipette for sample appli- cation and concentrate or filtrate retrieval 8.5.2 Centrifuges Unit Value Swing bucket rotor or fixed-angle rotor Minimum rotor angle for fixed-angle rotor 25° Rotor suitable for centrifuge tubes with the following properties Conical base... -
Page 21: Chemical Compatibility
Technical Specifications Chemical Compatibility Chemical, biological and aqueous solutions with appropriate compatibility for the materials of the product (2 hr contact time) Examples of compatible chemical solutions Acetic Acid (25%) Lactic Acid (5%) Acetonitrile (10%) Mercaptoethanol (10 mM) Ammonium Sulphate (saturated) Methanol (60%) N-Butanol (70%) Phosphate Buffer (1 M) -
Page 22: Typical Performance Characteristics
Technical Specifications Typical Performance Characteristics 8.8.1 Typical Concentration Conditions Centrifuge Centrifuge with swing with bucket rotor fixed-angle rotor Unit Value Value Centrifugation for recovery of > 90% of the dissolved sub- stance (typical) Concentration times 5 – 20 5 – 20 Concentration factor Buffer exchange and centrifu- Number... -
Page 23: Recoveries
Technical Specifications 8.8.2 Examples of Times to Concentrate 30x at 20 °C and Solute Recoveries Centrifuge with swing Centrifuge with bucket rotor fixed-angle rotor (25°) Time (min) Solute Time (min) Solute Recovery Recovery Lysozyme 1 mg/mL (14.3 kDa MW) 5 kDa Cytochrome c 0.25 mg/mL (12.4 kDa MW) 5 kDa α-Chymotrypsin 1 mg/mL (25 kDa MW) - Page 24 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.
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