Thermo Scientific 7451 Operating And Maintenance Manual page 12
Cryomed controlled rate freezer
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Also See for 7451:
- Operating and maintenance manual (120 pages) ,
- Manual (138 pages) ,
- Instruction sheet (5 pages)
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Pre-set Profiles
(continued)
Thermo Scientific
Pre-Set Profile #3 - Commonly used for straw size samples, resulting in a
slow rate to -7°C, a 5 minute hold for manual cell nucleation, a 0.3°C
rate to -35°C, and then a 0.1°C per minute cooling rate to a -37°C end
temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .Hold for 5m
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.3 C/m C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1 C/m C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #4 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 1°C rate from nucleation to -45°C and a
10°C per minute cooling rate to a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #5 - Commonly used for skin packets, resulting in a 1°C
rate from nucleation to -35°C and a 10°C per minute cooling rate to a
-90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Section 1
Introduction
at 20C
to -4.0C
to -7.0C
at -7.0C
to -35C
to -37C
at 20.0C
to -6.0C
to -50C
to -14.0C
to -45C
to -90C
at 4.0C
to -5.0C
to -40C
to -10.0C
to -35C
to -90C
Controlled Rate Freezer
1-5
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