Running A Performance Check - BD FACSCelesta Flow Cytometer User Manual

Running a performance check

Introduction
Overview
Considerations
Before you begin
This topic describes how to run a performance check as part of
quality control.
The CS&T application is designed to monitor performance on a
daily basis and to optimize laser delay.
Running a performance check on a regular basis provides a
standard for monitoring changes in performance due to
degradation of laser power, aging of PMTs, and other potential
cytometer service issues. Performance results are also affected by
fluidics performance. We strongly recommend following the
fluidics maintenance procedures as described in
fluidics (page 47).
Some BP filters might not be normalized to CS&T settings. In this
case, CS&T will generate Qr and Br numbers that are not
comparable from instrument to instrument. They are however, still
trackable on one cytometer. Part of the process for optimizing
cytometer settings includes verifying PMT voltages set by CS&T
for all parameters. Carefully examine any channel with a non-
CS&T normalized filter. If the baseline settings are not appropriate
for your applications, adjust the PMT voltage as necessary and use
the Application Settings to maintain Target Values.
Define the performance baseline for any configuration before
running a performance check. See
baselines (page 43).
Chapter 5: Optimizing cytometer settings
Cleaning the
Custom configurations and
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