BD FACSCelesta Series Job Aid

Job Aid
BD FACSCelesta™, BD LSRFortessa™, and
BD FACSymphony™ Analyzer
system using the BD FACSFlow™ Supply System
This job aid contains instructions for performing a system flush using the BD FACSFlow™ Supply System (FFSS) on BD LSRFortessa™,
BD FACSCelesta™, and BD FACSymphony™ Flow Cytometers. A system flush cleans the overall fluidics to remove debris and
contaminants from the sheath and waste tubing, and flow cell. Perform the system flush at least every two weeks. See your
instrument's user guide for additional information.
Before you begin
You will need the following items:
• Stainless steel sheath tank with dedicated
tubing lines
NOTE The cytometer system is shipped
with a stainless-steel tank for sheath fluid.
This tank is replaced by the sheath
cubitainer when the BD FACSFlow™ Supply
System is installed. You should save the
stainless-steel tank and tubing and use it
for the system flush procedure.
• 2 L of undiluted BD FACSClean™ Solution or
a freshly prepared 10% bleach solution
• 2 L of 1.5% BD
® Detergent Solution
• 4 L of deionized (DI) water
Preparing for the system flush

Place the stainless steel sheath tank near the cytometer.

On the right side of the cytometer, disconnect the air line
(green) from the air out port and the fluid line (blue) from
the fluid in port.

Connect the air line (green) from the stainless steel sheath
tank to the air out port on the cytometer then connect the
fluid line (blue) from the stainless steel sheath tank to the
fluid in port.

Empty the waste tank, if needed.
families: Flushing the