BD LSR II Benchtop Flow Cytometer Manuals

Manuals and User Guides for BD LSR II Benchtop Flow Cytometer. We have 4 BD LSR II Benchtop Flow Cytometer manuals available for free PDF download: User Manual, Workbook

BD LSR II User Manual (204 pages)

flow cytometer

Brand: BD | Category: Laboratory Equipment | Size: 7 MB

Table of Contents
5
About this Guide

11
- Conventions
  11
- BD LSR II Documentation
  12
  - BD LSR II Online Help
    12
  - Printed Documentation
    13
  - Electronic Documentation
    14
- Technical Assistance
  15
Safety and Limitations

17
- Laser Safety
  17
  - Laser Product Classification
    18
  - Precautions for Safe Operation
    18
- Electrical Safety
  19
- Biological Safety
  20
- General Safety
  21
- Symbols and Labels
  21
- Limitations
  23
Chapter 1: Introduction

25
- Overview
  26
- Components
  26
  - Power Switch
    26
  - Control Panel
    27
  - Handles
    27
- Fluidics
  28
  - Sample Flow Rate Control
    28
  - Fluid Control
    28
  - Sample Injection Port
    29
  - Sheath and Waste Containers
    31
- Optics
  32
  - Lasers
    33
  - Filters
    34
  - Detectors
    35
- BD LSR II Workstation
  36
Chapter 2: Instrument Setup

37
Chapter 3: Running Samples

51
- Sample Optimization Using Instrument Setup
  52
- Recording and Analyzing Data
  65
  - Preparing the Workspace
    65
  - Recording Data
    67
  - Analyzing Data
    69
  - Reusing the Analysis
    73
  - Saving the Analysis
    73
Chapter 4: DNA Analysis

75
- Criteria for DNA Experiments
  76
- DNA Setup
  77
  - How to Use DAPI with DNA QC
    77
  - How to Use PI with DNA QC
    78
- CEN Optimization
  78
  - Preparing the Workspace
    78
  - Running CEN
    82
- CTN Resolution
  84
  - Running CTN
    84
- Optimization for Data Recording
  86
Chapter 5: Calcium Flux

87
Chapter 6: Maintenance

99
- Daily Cleaning and Shutdown
  100
  - Daily Fluidics Cleaning
    100
  - Daily Shutdown
    102
- Scheduled Maintenance
  103
  - System Flush
    103
  - Waste Management System Maintenance
    105
- Periodic Maintenance
  107
  - Changing the Sheath Filter
    108
  - Changing the Bal Seal
    110
  - Changing the Sample Tube O-Ring
    112
Chapter 7: Troubleshooting

113
- Instrument Troubleshooting
  114
Appendix A: Technical Overview

123
- Fluidics
  124
- Optics
  125
  - Light Scatter
    125
  - Fluorescence
    126
  - Optical Filters
    127
  - Compensation Theory
    131
- Electronics
  134
  - Pulse Measurements
    135
  - Digital Electronics
    136
  - BD Facsdiva Option White Paper
    136
  - Threshold
    137
  - Laser Controls
    137
Appendix B: BD LSR II QC Log

139
Appendix C: Filter Templates

143
- Octagon Template
  145
- Trigon Template
  147
Appendix D: Supplies and Consumables

149
- QC Particles
  150
- Reagents
  151
- Equipment
  152
Appendix E: Standard Default Configuration

153
- Standard (4-Blue 2-Violet 2-UV 2-Red) Configuration Specification
  154
  - Default Configuration
    155
  - Octagon and Trigon Maps
    156
- Custom Configurations
  159
Appendix F: Special Order Configurations

165
Appendix G: Setting Laser Delay

193
- About Laser Delay
  194
- Optimizing Laser Delay
  195

BD LSR II User Manual (184 pages)

High Throughput Sampler

Brand: BD | Category: Laboratory Equipment | Size: 6 MB

Table of Contents
5
About this Guide
9
- Conventions
  10
- Technical Assistance
  11
Chapter 1: Introduction

13
- HTS Hardware Overview
  14
- Components
  16
- Cytometer Connections
  19
  - Cytometer Interface Panel
    19
  - Sample Coupler
    21
- Sample Processing
  23
Chapter 2: BD Facsdiva Software Overview

27
- Workspace Components
  28
- Plate Window
  32
  - Setup View Components
    33
  - Setup View Functions
    43
  - Analysis View Components
    48
  - Analysis View Functions
    51
Chapter 3: Running Samples

55
- Starting up
  56
  - Setting up for Plate-Based Acquisition
    59
- Creating Experiments
  64
  - Creating a Folder and an Experiment
    64
  - Setting up the Plate
    67
  - Setting up the Worksheet
    74
  - Exporting a Plate as a Template
    76
  - Assigning Keywords
    77
- Preparing for Acquisition
  78
  - Optimizing Cytometer and HTS Settings
    78
  - Performing Compensation
    81
- Acquiring Data
  82
- Analyzing Data
  86
  - Displaying Keywords
    86
  - Performing Data Analysis
    90
  - Maintaining Data
    93
- Returning to Tube-Based Acquisition
  94
  - Returning to Tube-Based Acquisition on the BD LSR II
    94
  - Returning to Tube-Based Acquisition on the BD Facscanto
    97
- Shutting down
  100
  - Quitting the Software
    101
Chapter 4: Maintenance

103
- Daily Maintenance
  104
  - Daily Cleaning
    104
  - Cytometer Inspection and Servicing
    109
- Monthly Maintenance
  111
  - Monthly Cleaning
    111
  - Surface Inspection and Cleaning
    114
- Periodic Maintenance
  114
- Unscheduled Maintenance
  136
  - Homing the Sample Probe
    136
  - Priming the HTS
    138
  - Performing a Motion Test
    138
  - Verifying the Sample Probe Position
    140
  - Reinitializing the HTS
    142
  - Inspecting Thumbscrew Fittings
    142
  - Inspecting Hexagonal Fittings
    143
  - Declogging the SIT
    143
Chapter 5: Troubleshooting

145
- HTS Troubleshooting
  146
- Acquisition Troubleshooting
  147
- BD Facsdiva Troubleshooting
  149
Appendix A: Consumables and Replacement Parts

151
- Cytometer Supplies
  152
- Maintenance Log
  154
Appendix B: Depot Repair Procedures

157
- Depot Repair Overview
  158
- Removing the HTS Unit
  159
- Cleaning the HTS Unit
  162
  - Decontaminating the Fluidics Manually
    163
  - Decontaminating External Surfaces
    167
- Unpacking the Replacement Unit
  169
  - Lifting Heavy Objects
    169
  - Unpacking the HTS Unit
    170
- Installing the HTS Unit
  170
- Packing the Unit for Shipping
  175
  - Placing the HTS into Its Shipping Container
    175
- Index
  177

BD LSR II Workbook (84 pages)

Brand: BD | Category: Measuring Instruments | Size: 0 MB

Table of Contents
5
1 Introduction

7
- About the Instrument
  9
  - Power Switch
    9
  - Control Panel
    10
  - Fluidics
    10
  - Optics
    15
  - Electronics
    20
  - Software
    20
- Overview
  21
2 Operations and Maintenance

25
- Lab Exercise: BD LSRII Startup
  27
- Lab Exercise: BD LSRII Shutdown
  31
- BD LSRII Maintenance and Care Procedures
  33
  - Regular Maintenance
    33
  - Periodic Maintenance
    37
  - Laser Maintenance
    42
3 Optics and Lasers

43
- Lasers
  45
  - Optical Filters
    46
  - Optical Filters in the BD LSRII
    46
4 Instrument Quality Control

55
- Instrument Quality Control
  57
- Lab Exercise: Performing Quality Control
  58
  - Preparing the QC Sample
    58
Appendix A Worksheets

65
Appendix B Troubleshooting

71
BD LSR Overview Course Evaluation

81

BD LSR II User Manual (9 pages)

Flow Cytometry Analyzer

Brand: BD | Category: Measuring Instruments | Size: 0 MB

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BD LSR II Benchtop Flow Cytometer Manuals

BD LSR II User Manual (204 pages)

Table of Contents

About this Guide

Safety and Limitations

Chapter 1: Introduction

Chapter 2: Instrument Setup

Chapter 3: Running Samples

Chapter 4: DNA Analysis

Chapter 5: Calcium Flux

Chapter 6: Maintenance

Chapter 7: Troubleshooting

Appendix A: Technical Overview

Appendix B: BD LSR II QC Log

Appendix C: Filter Templates

Appendix D: Supplies and Consumables

Appendix E: Standard Default Configuration

Appendix F: Special Order Configurations

Appendix G: Setting Laser Delay

BD LSR II User Manual (184 pages)

Table of Contents

Chapter 1: Introduction

Chapter 2: BD Facsdiva Software Overview

Chapter 3: Running Samples

Chapter 4: Maintenance

Chapter 5: Troubleshooting

Appendix A: Consumables and Replacement Parts

Appendix B: Depot Repair Procedures

BD LSR II Workbook (84 pages)

Table of Contents

1 Introduction

2 Operations and Maintenance

3 Optics and Lasers

4 Instrument Quality Control

Appendix A Worksheets

Appendix B Troubleshooting

BD LSR Overview Course Evaluation

BD LSR II User Manual (9 pages)

Table of Contents

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