BD FACSCanto II Quick Reference Manual
Flow cytometer
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Also See for FACSCanto II:
- Instructions for use manual (250 pages) ,
- User manual (184 pages) ,
- Reference manual (158 pages)
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BD FACSCanto II Flow Cytometer
This guide contains instructions for both BD FACSCanto™ clinical and
BD FACSDiva™ software. Follow only those sections that apply to the
software you are running.
Starting Up
1
Turn on the cytometer main power.
2
Start up the computer, launch the software, and
log in.
3
Make sure the software is connected to the cytometer; if needed,
connect manually (choose Cytometer or Instrument > Connect).
4
Check fluid levels in the Status or Instrument window.
Empty the waste or replace fluid containers, if needed.
5
If automatic cleaning is disabled, run the fluidics startup procedure.
To prevent fluid overflow, make sure there is no tube on the sample
injection tube (SIT) during fluidics startup.
6
Check the flow cell for air bubbles; if needed, run the de-gas flow cell
procedure.
7
Check that laser warmup has finished.
Quick Reference Guide
BD FACSDiva software
BD FACSDiva software
1
or
BD FACSCanto clinical software
BD FACSCanto clinical software
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Table of Contents
Related Manuals for BD FACSCanto II
Summary of Contents for BD FACSCanto II
- Page 1 BD FACSCanto II Flow Cytometer Quick Reference Guide This guide contains instructions for both BD FACSCanto™ clinical and BD FACSDiva™ software. Follow only those sections that apply to the software you are running. Starting Up Turn on the cytometer main power.
- Page 2 Daily Setup: BD FACSCanto Clinical Software Set up the cytometer with BD FACSCanto clinical software, even if you will optimize and run samples using BD FACSDiva software. Prepare BD FACS™ 7-color setup beads. Run the cytometer setup procedure for the bead lot you prepared.
- Page 3 Report for optimized settings. Click , remove the tube (if prompted), and then click Optimizing Instrument Settings: BD FACSDiva Software Before you record data for a sample, optimize the PMT voltages, compensation, and threshold settings for the sample type and fluorochrome.
- Page 4 Adjust the gates around the positive populations on the histogram for each stained control tube. Calculate compensation, and name the resulting setup. Running Samples: BD FACSCanto Clinical Software Prepare a worklist. Install the first sample tube (manual acquisition) or the Loader rack (automated acquisition), and click When asked if you want to save the worklist, click No.
- Page 5 Choose File > Exit, and select Run fluidics shutdown and exit. When fluidics shutdown completes, turn off the power. Shutting Down: BD FACSDiva Software Install a tube with ≤3 mL of BD FACSClean solution or 10% bleach, and click After 5 minutes, click and remove the tube.
- Page 6 Tel (32) 53-720211 Tel (52) 55-5999-8296 Fax (65) 6-860-1590 Fax (55) 11-5185-9895 Fax (32) 53-720450 Fax (52) 55-5999-8288 BD, the BD logo, BD FACS, BD FACSDiva, and BD FACSCanto are trademarks of Becton, Dickinson and Company. 640772 © 2006 BD...
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