BD LSRFortessa User Instructions

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Flow Cytometry User Instructions
at the University of Konstanz
FACSFortessa
The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer
provides rapid, fully automated sample acquisition from microtiter plates. In high-
throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes.
Standard throughput mode can be selected for acquisition of larger sample volumes.

Preparing the HTS

Switch the acquisition control to plate mode.

This will switch off the DCM pump.
Unscrew the tube retainer (grey ring

DCM sleeve onto the SIT and carefully remove the sleeve.
Remove the DCM sleeve and replace it with the shorter SIT

protector.
Install the sample coupler on the SIT.

Check the level in the connected water bottle (> ~30 mL).

Switch on the HTS reader (on the backside).

Press the Run button (the HTS does not operate in STNDBY mode).


Initialisation and Priming the HTS

Note: Don't change PMTV of fluorescence channels after enabling compensation.
Operation of the loader is only possible via DIVA software – do not use the prime

button on the instrument.
When the BD DIVA software is already open, go HTS

Start BD DIVA software. The HTS will initialize automatically, otherwise go to HTS

Re-Initialize

Prime the HTS (Sheath tubing connected): HTS


Run your Experiment

See detailed instructions on HTS Programming.
– HTS User Instructions
TM
) that holds the
Re-Initialize.

Prime. 3x in total.
