Appendix; Principle Of Operation - Siemens Hematek 3000 System Instruction Manual

9

Appendix

9.1

Principle of operation

Staining glass slides
Buffering glass slides
Rinsing glass slides
Drying glass slides
Delivering glass slides
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Hematek 3000 System – Instruction Manual – Version 1.3
This chapter describes the principle of operation of the system and the
technical specifications of the system.
As the glass slides move along the platen, with the smeared side down, the
system performs the following steps:
When a glass slide contacts the first of three sensing switch fingers, the
respective sensing switch is activated. After a specific time delay, the stain
pump starts delivering the stain. The capillary space between the glass slide
and the platen is filled.
The stain pump delivers a specific amount of stain, according to the setting
of the stain adjustment knob. The surplus of stain is drained into the waste
tank.
When the glass slide contacts the second sensing switch finger, the
respective sensing switch is activated. After a specific time delay, the buffer
pump starts delivering the buffer. The capillary space between the glass
slide and the platen is filled. The stain and buffer move around the mixing
grooves and hence fully intermingle.
The buffer pump delivers a specific amount of buffer according to the setting
of the buffer adjustment knob. The surplus of buffer is drained into the waste
tank.
When the glass slide contacts the third sensing switch finger, the respective
sensing switch is activated. After a specific time delay, the rinse pump starts
delivering the rinse. The capillary space between the glass slide and the
platen is filled. The rinse washes the glass slides from the stain and buffer
mixture.
The rinse pump delivers a specific amount of rinse, according to the setting
of the rinse adjustment knob. The surplus of rinse is drained into the waste
tank.
After rinsing is completed, the glass slide is dried by a flow of air from a low
velocity blower.
The glass slide is delivered into the slide drawer.
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