Siemens Hematek 3000 System Instruction Manual page 82

8 Troubleshooting
8
Adjust amount of buffer
8 10
• If the stain overflows on either side of the smeared glass slide, turn the
stain adjustment knob counter-clockwise, see Figure 8-4.
1 Rinse adjustment knob
2 Buffer adjustment knob
3 Stain adjustment knob
4 Rear conveyor spiral
Figure 8-4 Adjusting the pump volumes
5. Check if proper fill has been achieved on two consecutive smeared
glass slides.
• If so: Go to step 6.
• If not: Repeat step 4 and step 5.
6. Load three priming slides into the grooves of the conveyor spirals with
the marked side facing in the opposite direction of conveyance.  The
system primes the platen and performs the staining.
7. Load five smeared glass slides immediately following the priming slides.
8. Adjust the amount of buffer.
Note: When a glass slide passes the buffer section of the platen, mixing
of the stain and buffer occurs because of the mixing grooves. This is
where the proper stain-to-buffer ratio is most important. The glass slide
looks under-filled as it travels over the mixing grooves because the
capillary gap is slightly greater.
• If the glass slide is past the mixing grooves and moves on to the smooth
portion of the platen, immediately before the rinsing area, and the stain
and buffer mixture does not fill the capillary space under the glass slide,
then turn the buffer adjustment knob clockwise, see Figure 8-4.
• If the stain and buffer mixture extends out from either side of the
smeared glass slide, turn the buffer adjustment knob counter-clockwise,
see Figure 8-4.
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Hematek 3000 System – Instruction Manual – Version 1.3