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Summary of Contents for Qiagen DML 3000
- Page 1 November 2022 DML 3000 User Manual For use with the digene ® Hybrid Capture ® 2 DNA tests 5000-00031 QIAGEN 19300 Germantown Road Germantown MD 20874 QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden GERMANY 1128779 Sample to Insight...
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Page 2: Table Of Contents
Contents Introduction ..............................4 About this user manual ......................... 4 General information..........................4 Intended use of the DML 3000 ......................5 Requirements for DML 3000 users ......................5 Materials required ..........................5 Materials required but not provided ....................... 5 Safety Information ............................6 Proper use ............................ - Page 3 Moving the transport locks to the home position ..................31 Placing a microplate in the DML 3000 ....................32 Securing the DML 3000 using the transport locks .................. 33 Repackaging and transporting the DML 3000 ..................33 Maintenance .............................. 36 Cleaning the DML 3000 ........................
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Page 4: Introduction
QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques. -
Page 5: Intended Use Of The Dml 3000
1.3 Intended use of the DML 3000 The DML 3000 is intended to measure light that is emitted as a result of a chemiluminescent reaction. Assay results obtained using chemiluminescence technology in 96-well microplates are calculated and interpreted according to assay validation parameters. -
Page 6: Safety Information
2 Safety Information Before using the DML 3000, it is essential that you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition. -
Page 7: Electrical Safety
Refer to digene HC2 System Software User Manual for other warnings and precautions related to operating the DML 3000. For best performance of the DML 3000, plug the DML 3000 into a surge protector or uninterruptible power supply (UPS) before plugging it into a grounded power source. -
Page 8: Environment
Venting for fumes and disposal of wastes must be in accordance with all national, state, and local health and safety regulations and laws. 2.6 Waste disposal For more information about how to dispose of the DML 3000, see “Waste Electrical and Electronic Equipment (WEEE)” on page 45. Hazardous chemicals and infectious agents WARNING The waste contains samples and reagents. -
Page 9: Mechanical Hazards
Damage to the instrument WARNING If the DML 3000 is powered ON, the optical head must remain in the home position while the access door is open. Attempting to move the optical head when the DML 3000 is powered ON exposes the optical head to ambient light. - Page 10 Type plate on the back of the instrument Unique device identifier (UDI) and instrument box label Instrument box label Relative humidity Instrument box label Barometric pressure Instrument box label Temperature range The front cover of this user manual Material DML 3000 User Manual 11/2022...
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Page 11: Additional Symbols Used In This User Manual And Instrument Box Label
Community 3 General Description The DML 3000 is highly sensitive with a broad dynamic range. It features a low background photon-counting PMT and measures light in the visible spectrum (350–650 nm). Microplates are loaded at the front of the DML 3000. The individual microplate wells are sequentially moved beneath the opening of the detector and then individually measured. - Page 12 Figure 1. Front view of the DML 3000 DML 3000 User Manual 11/2022...
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Page 13: Led Status Light
Figure 2. View of the LED status lights. (1): Red Led status light (2): Yellow Led status light (3): Green Led status light 3.2 Access door The access door is on the top of the DML 3000. It allows access to the plate mask and plate carrier. To open the door, lift the lower front edge. -
Page 14: Gas Actuator Lift Arm
Figure 4. View of the gas actuator lift arm 3.4 Optical sensor An optical sensor in the front-center of the DML 3000 detects if the door is open or closed. Figure 5. View of the optical sensor when the access door is opened. (1): Optical sensor (2): Flag If the access door is not completely closed, the optical sensor does not detect the flag and the digene HC2 System Software reports an open door error. -
Page 15: Optical Head
The optical head measures the chemiluminescence of each microplate well. The optical head has an optical mask and an optical mask retainer. The optical mask isolates one microplate well from the other microplate wells so that the DML 3000 can measure only that one microplate well. -
Page 16: Plate Mask
Figure 8. View of the plate mask when the access door is opened 3.8 Plate mask release latch The plate mask release latch is used to open the plate mask, which opens approximately 90 degrees. Figure 9. View of the plate mask release latch DML 3000 User Manual 11/2022... -
Page 17: Microplate Locator
Figure 10. View of the microplate locator. (1): Microplate locator (2): A1 orienting corner 3.10 Detector The DML 3000 uses a single PMT to measure chemiluminescence of the microplate wells on a 96-well microplate. It leverages both photon-counting and current-measurement modes to optimize the dynamic range of the DML 3000. -
Page 18: Transport Locks
Sensitivity describes the lowest intensity of light that can be detected as different from background. Sensitivity is usually measured using the amount of a luminescent substance the DML 3000 is able to detect and clearly distinguish from background noise. 3.10.4 Dynamic range Dynamic range is the relationship between the lowest and the highest signal that the detector is able to quantify. - Page 19 The DML 3000 is shipped from QIAGEN with the transport locks in the home position. The foam-block insert secures the plate carrier and optical head during transport. The transport locks are only used for transport when the foam-block insert is not available.
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Page 20: Power Connection Port And Power Switch
3.12 Power connection port and power switch Located at the back-left of the DML 3000, the power connection port is where the power adapter plugs into the DML 3000. The power switch powers the DML 3000 ON and OFF. Figure 13. View of the power connection and the power switch. (1): Power connection port (2): Power switch 3.13 Power cord... -
Page 21: Power Adapter
3.14 Power adapter The power adapter connects to the DML 3000 power connection port at one end and to the power cord at the other end. Important: Only use the power adapter provided with the DML 3000. Use of a power adapter other than the one provided with the DML 3000 voids the warranty. -
Page 22: Unpacking Procedures
To prevent injury, bend your knees and lift the DML 3000 slowly to compensate for this weight difference. The DML 3000 must be unpacked from its shipping container. Place the box on an even surface. One person should be able to unpack the DML 3000 and lift it from the packing box. - Page 23 Figure 18. View of the packing foam removed from around the DML 3000 10. Remove the tape on the plastic shipping bag. 11. Remove the DML 3000 from the plastic shipping bag, discard the 2 desiccant bags in the plastic shipping bag, and place the plastic shipping bag into the shipping box.
- Page 24 12. Open the DML 3000 access door. Locate the foam-block insert on the right side of the DML 3000. Figure 20. View of foam-block insert inside the DML 3000 13. Remove the foam-block insert from the DML 3000, and place it in the shipping box.
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Page 25: Installation Procedures
For power requirements and technical data, see “Appendices,” page 44. The DML 3000 type plate is located on the right side of the back panel next to the power switch. The type plate contains the DML 3000 serial number which is required to set up the DML 3000 in the digene HC2 System Software. - Page 26 Note: The power connection port is located on the right side of the back panel. Important: Only use the power adapter provided with the DML 3000. Use of a power adapter other than the one provided with the DML 3000 voids the warranty.
- Page 27 Note: If a RCS or LIS is used on the same computer as the DML 3000, then a USB-to-serial converter will be required. The USB-to-Serial converter is supplied with the Personal Computer Country Kit. Refer to digene HC2 System Software User Manual for the correct COM port to use and setting up the connections.
- Page 28 Figure 24. Diagram of connecting a DML 3000 with an RCS. DML 3000 User Manual 11/2022...
- Page 29 Figure 25. Diagram of connecting a DML 3000 with a LIS. DML 3000 User Manual 11/2022...
- Page 30 Figure 26. Diagram of connecting a DML 3000 with a RCS and a LIS. DML 3000 User Manual 11/2022...
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Page 31: General Operation
6.1 Moving the transport locks to the home position The transport locks must be located in the home position to operate the DML 3000. Use this procedure to move into the transport locks to the home position. 1. Open the access door. -
Page 32: Placing A Microplate In The Dml 3000
2. Press the plate mask release latch to lift the plate mask. CAUTION Damage to the instrument Insert the microplate in the DML 3000 so there is no interference from the microplate locator. If necessary, lift the plate mask 90 degrees to make sure there is no interference. -
Page 33: Securing The Dml 3000 Using The Transport Locks
2. Slide the transport lock on the optical head guide rail all the way to the right, and tighten the thumb screw. 3. Slide the transport lock on the left guide rail to the back of the DML 3000, and tighten the thumb screw. - Page 34 8. Place the foam-block insert into the space between the plate carrier guide rails. If a foam-block insert is not available, use the transport locks to secure the internal components of the DML 3000 during transport. See Figure 27 on page 32 for additional instructions.
- Page 35 CAUTION Damage to the instrument If the internal components of the DML 3000 are not secured, severe damage to the DML 3000 will result and the warranty will be voided. 9. Once the internal components are secure, close the access door.
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Page 36: Maintenance
Cleaning the exterior of the DML 3000 At least once per week, wipe the exterior of the DML 3000 with a low-lint paper towel moistened with 70% isopropyl alcohol or 0.5% bleach solution. If a 0.5% bleach solution is used, follow by wiping with a low-lint paper towel moistened with deionized or distilled water. - Page 37 If liquid has spilled inside the DML 3000 or liquid is visible on the plate mask or the underside of the plate mask, wipe up the excess liquid with a low-lint paper towel, and clean the interior of the instrument as described below.
- Page 38 Figure 34. Wiping the interior of the plate mask 3. Clean each opening in the plate mask with a foam-tipped applicator or cotton swab wrapped in a low-lint paper towel moistened with 70% isopropyl alcohol. Figure 35. Cleaning the plate mask openings DML 3000 User Manual 11/2022...
- Page 39 4. Gently push the plate carrier to the back of the DML 3000. Figure 36. Pushing the plate carrier 5. Gently push the optical head from the home position toward the center of the instrument. Figure 37. The optical head in the home position...
- Page 40 Do not touch the bottom surface of the optical head while removing the mask. 7. Place your fingers on both sides of the optical head, grasp the optical mask, and gently pull the optical mask toward you to remove it. DML 3000 User Manual 11/2022...
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Page 41: Decontaminating The Dml 3000
7.2 Decontaminating the DML 3000 To decontaminate the DML 3000, clean the interior of the instrument. See “Cleaning the interior of the DML 3000” on page 36 for additional instructions. Once the cleaning is complete, wait at least 10 minutes before wiping the interior of the instrument with a low-lint paper towel moistened with deionized or distilled water. -
Page 42: Troubleshooting
When contacting QIAGEN Technical Services about an error with the DML 3000, note the steps leading up to the error and any information appearing in any dialog boxes. This information will help the QIAGEN Technical Services solve the problem. - Page 43 “Erratic Results” 1. If the DML 3000 is not powered ON at all The DML 3000 is powered ON but has not message times, allow the DML 3000 to warm up for at...
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Page 44: Appendices
9.1.4 Mechanical data and hardware features Dimensions (h x w x d) 26.1 x 49.3 x 51.0 cm (10.3 x 19.4 x 20.0 in.) Height of DML 3000 with access 51 cm (20 in.) door open Weight 10 kg (22 lbs.) -
Page 45: Legal
Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE- marked electronic equipment is provided. - Page 46 QIAGEN is not responsible for any radio television interference caused by unauthorized modifications of this equipment or the substitution or attachment of connection cables and equipment other than those specified by QIAGEN. The correction of interference caused by such unauthorized modification, substitution, or attachment will be the responsibility of the user.
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Page 47: Dml 3000 Maintenance Log
9.3 DML 3000 Maintenance Log Use this log to record routine maintenance of the DML 3000 according to your laboratory’s policies and procedures. As needed and weekly maintenance of DML 3000 Task Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date Initial/Date As Needed... -
Page 48: Ordering Information
10 Ordering Information Product Contents Cat. no. DML 3000 The DML 3000 is a luminometer that automates amplified 5000-00031 chemiluminescent signal detection and results reporting for digene Hybrid Capture 2 DNA Tests. Related Products Lumicheck Plate Tool used to monitor the performance of the digene microplate 6000-5013 luminometer (DML). -
Page 49: Document Revision History
The LumiCheck Plate, its components, or its method of use may be covered by the following patent and its international counterpart: U.S. Patent No. 6,335,997 Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. 11/2022 © 2022 QIAGEN, all rights reserved. DML 3000 User Manual... - Page 50 Ordering www.qiagen.com/contact | Technical Support support.qiagen.com | Website www.qiagen.com DML 3000 User Manual 11/2022...