Hanna Instruments HI6221 Instruction Manual
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Hanna Instruments HI6221 Instruction Manual

Ph and orp benchtop meter
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pH and ORP
Benchtop Meter

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Summary of Contents for Hanna Instruments HI6221

  • Page 1 HI6221 pH and ORP Benchtop Meter...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. Preliminary Examination ....................5 2. Safety Measures ....................... 6 3. Abbreviations ........................6 4. User Interface – Icons ...................... 7 5. General Description & Intended Use ................... 9 5.1. Main Features ...................... 10 6. Specifications ......................... 11 6.1.
  • Page 4 10.6. Logging ......................52 10.7. Profiles ......................53 11. Calibration ........................55 11.1. pH Calibration ..................... 56 11.2. Relative mV Calibration ..................60 12. Measurement ....................... 63 12.1. Measurement Tips ....................63 12.2. Direct Readings ....................63 12.3. Direct / Autohold Readings .................. 64 13.

  • Page 5: Preliminary Examination

    1. PRELIMINARY EXAMINATION Each HI6221 pH and ORP benchtop meter is supplied with: • HI1131B pH electrode • HI7662-TW temperature probe • pH calibration starter kit consisting of: pH 4.01 buffer solution sachet (2 pcs.) • pH 7.01 buffer solution sachet (4 pcs.) •...

  • Page 6: Safety Measures

    2. SAFETY MEASURES Handling precautions The unit, while not fragile, can be damaged by improper usage and handling. • Do not puncture or drop the unit. • Keep the unit on a stable and even surface, away from contact with liquid. •...

  • Page 7: User Interface - Icons

    4. USER INTERFACE – ICONS Capacitive keys Description Home key Back key Menu key Symbol on display Description Measurement Menu Available log storage Log Recall System Menu Users Help Start logging Stop logging Manual logging pH / ORP electrodes Log recall, modify settings Log recall, graph view - function not selected Log recall, graph view - function active Log recall, table view - function not selected...
  • Page 8 Symbol on display Description Autohold applied Autohold, waiting stable pH measurement Autohold, waiting stable mV measurement Triggers log session, pending next stable measurement Autohold logging in progress Forward/backward navigation, sequence of steps - function not available Active buffer selection during manual or semiautomatic calibration Calibration procedure, buffer selection - function available Calibration procedure, buffer selection - function not available Connection status...

  • Page 9: General Description & Intended Use

    / ORP measurement module. Compact and easy to operate, the benchtop meter is delivered with Hanna Instruments HI1131B double junction combination pH electrode, together with HI7662-TW temperature probe.

  • Page 10: Main Features

    • The Measurement Setup icon ( ), found in measurement screen, opens sensor setup parameters. • The Log Recall icon ( ) recalls storred log sessions (Automatic continuous logging, Manual, or Autohold). 5.1. MAIN FEATURES Measurement & Calibration • Measure pH/mV (pH) or mV/Rel.mV (ORP) with temperature •...

  • Page 11: Specifications

    6. SPECIFICATIONS 6.1. INSTRUMENT -2.0 to 20.0 pH -2.00 to 20.00 pH -2.000 to 20.000 pH Range ±2000.0 mV -20.0 to 120.0 °C Temperature -4.0 to 248.0 °F 253.0 to 393.0 K 0.1 pH 0.01 pH 0.001 pH Resolution 1 mV 0.1 mV Temperature 0.1 °C / 0.1 °F / 0.1 K...
  • Page 12 Calibration points Up to 5 Automatic Type Semiautomatic Manual Hanna and NIST Standard buffers pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 pH Calibration Custom buffers Up to 5 Custom group Up to 5 calibration point Offset or Points (user setting) Disabled Reminder Daily: 0 min.
  • Page 13 USB-A 2 ports for keyboard input or USB thumb drive USB-C 1 port for PC connectivity and USB-C type thumb drive Connectivity Wi-Fi & Ethernet Web server Log transfer and download Email RS232 Connecting peripherals Power supply DC adapter 100-240AC to 24VDC 2.5A 0 - 50 °C / 32 - 122 °F / 273 - 323 K Environment maximum 95% RH non-condensing...

  • Page 14: Electrodes

    6.2. ELECTRODES HI1131B — pH electrode Range 0 to 14 pH Reference cell type Double, Ag/AgCl Junction type Ceramic Single 15-20 µL per h Refill electrolyte 3.5M KCl Maximum pressure 0.1 bar Body material Glass Tip shape Spheric (Ø 9.5 mm) Recommended operating temperature 0 to 100°C (32 to 212°F) - HT Temperature sensor...

  • Page 15: Functional & Lcd Description

    8. Buffer trays 4. pH reading 9. Temp. reading and Temp. compensation status 5. mV reading 10. Start Logging icon 6. pH electrode icon 11. Measurement Menu icon 12. USB connection status and Logging space availability 13. User name (default “HI6221”)
  • Page 16 Home key • performs an exit or escape function • direct key for the system’s Menu items Menu key • permits access to the system’s settings, log recall, help, and users configuration REAR view HI6221 USB-C Temp. pH/ORP USB-A Ethernet ON/OFF Ref.

  • Page 17: Electrodes

    7.2. ELECTRODES HI1131B – pH electrode HI7662-TW – Temperature probe 1. Reference wire 2. Inner reference junction 1. Cable 3. Double, Ag/AgCl reference cell type 2. Handle 4. Reference fill cap 3. Stainless steel tube 5. Outer reference junction 6. Glass bulb...

  • Page 18: Getting Started

    8. GETTING STARTED 8.1. ATTACHING THE HI764060 ELECTRODE HOLDER Attaching the electrode stand and arm • Take the HI764060 electrode holder from the box. • Identify the metal base plate (4) with the integrated pivot pin (5) and the screw (2). •...
  • Page 19 4. To open the latch, press the clip inward while pulling up the latch. Insert clip Open clip Close clip press pull Easy to install and able to accomodate several cables, the clip secures the cabling by allowing cables to move freely with the arm motion. Using the adapter The electrode holder at the end of the arm has three different-sized apertures: •...

  • Page 20: Using The Electrode Holder

    ORP half-cell sensors and separate reference electrode with suitable jack connectors can also be used. Hanna Instruments pH electrodes with integral temperature elements are also supported. HI1131B glass-bodied combination pH electrode is connected through a BNC connector, which makes attaching and removing the probe an easy process.

  • Page 21: Powering The Unit

    2. Connect the power plug into the 24V power socket. 3. Press the black ON/OFF power button located on the back of the meter case, to power the instrument. At start up, the meter briefly displays the initialization screen (the Hanna Instruments logo, meter name, and bootloader version).

  • Page 22: Basic Operations

    8.5. BASIC OPERATIONS • The general operating modes are setup, measurement, logging, and data sharing. • Tap direct Menu key ( ) on the unit and tap: Users setup icon ( ) to open user menu. • System Menu icon ( ) to access meter settings.

  • Page 23: System Menu Items

    9. SYSTEM MENU ITEMS Tap direct Menu key ( ) on the unit (users must have previously logged in) for the System Menu screen to be displayed. System Menu icons & brightness control bar Symbol Name Functionality Users Tap to login and start configuring rights and instrument accessibility. Settings Tap to start configuring system and connectivity items.

  • Page 24: Users

    3. From Measurement screen, tap Menu key ( ) on the unit. 4. From System Menu screen, tap Users ( ) on display. 5. Tap Logout on display to enter Users screen. As a default setting, HI6221 Admin account (with built-in administrator rights) is automatically created.
  • Page 25 6. From Users screen, tap the plus symbol avatar. 7. Use the on-screen keypad to enter user name for new account. 8. Tap Enter on keypad to confirm. Log out & Switch User 1. From System Menu screen, tap Users ( ) on display.
  • Page 26 Account Management From Account Management screen users with administration rights can complete one or all of the following actions: • Enable Account Creation • Enable Logins Each power up requires user selection before instrument enters measurement mode. • Enable Admin i.e. assign administration rights to a standard user. Note: For all three enable/disable functions, tap switch on display to toggle options.
  • Page 27 Tap Delete. The instrument prompts for confirmation.

  • Page 28: Settings

    9.2. SETTINGS Settings is the second item under the System Menu. The function allows users to configure network connection and architecture, connectivity services, change system settings, and view Meter information. Network, Connectivity, and System tabs permit users to navigate through all system setting options and operations.
  • Page 29 9.2.1. Network Options: Disabled, Ethernet, Wi-Fi This selection determines how measurement logs are shared. The user can select network to be connected via Ethernet or Wi-Fi or Disabled. With connection enabled, the IP assignment can be set to Dynamic or Static. For setting a Static IP address: 1.
  • Page 30 • Meter web server, permits log file download to a web client. • Sending emails, permits log files to be transferred by email. Note: Email address is entered under User. 9.2.3. System Options: Time, Date, Language, Meter ID, Decimal Separator, Backlight Savers, Beepers, Startup Tutorial, Factory Settings Note: Use the scroll bar to view or select from entire settings list.
  • Page 31 Set Automatically • Direct selection from scrollable list of options • UTC options: from UTC 00:00 to UTC+14:00 from UTC 00:00 to UTC -12:00 (half hour increments) 24 Hour (Set Manual) • Direct selection from scrollable lists of options • Hour, Minute, Second, Date, and Date Format DST (Daylight Savings Time) seasonal time change is used in some locations that advances clocks (typically by one hour) during warmer months.
  • Page 32 Tap on-screen switches (enable or disable function) or direct buttons (direct selection) for following settings: Decimal Separator Select comma or period for regional preference. Backlight Saver Enable or Disable option. Select from 1 to 60 minutes. Note: If the backlight turns off after the set period of time, tap screen to turn it back on. Beeper –...
  • Page 33 Factory Settings Option restores System settings to their original factory values. This includes, resolution for measured data, temperature unit, view mode, alarm. Restoring factory settings deletes all user information, logs, or stored Profiles. When option invoked, the instrument asks for confirmation. Note: Only visible to user with Administrative rights.

  • Page 34: Log Recall

    The item allows users access and management (selection, deletion, and sharing) of measurement data. Only the user who generated the data has access to the logs created by that user. Depending on selected reading parameter (HI6221 supports pH, mV, and Rel.mV parameters ), logged data is stored in parameter-specific csv. files.
  • Page 35 4. Tap graph ( ) or table ( ) icon on display, to have logged data either plotted on a graph or displayed in a tabulated form. Tap to modify the temperature unit. When shared, the logged files record the temperature unit selected at the time measurement was taken.
  • Page 36 Tap information icon ( ) on display for user and profile name, instrument name and serial number, channel, lot information, as well as GLP data. Select All / Deselect All Select the option to export all logs to a USB-A flash drive. 1.
  • Page 37 Share Options USB-A and USB-C Plug the USB flash drive into the unit in one of the USB ports located on the back. 1. From System Menu, tap Log Recall symbol ( ) on display. Log History screen opens up. 2.
  • Page 38 Email Make sure the meter is connected to the internet and the Enable sending emails option enabled (see Connectivity section). In Users menu ( ), type the email address in the required field. 1. From System Menu, tap Log Recall symbol ( ) on display.
  • Page 39 Web server Use any browser to access the web server and download log files and tags. Make sure the HI6221 connected to the internet and the Enable meter web server option is active (see Connectivity section). Note: Both the meter and the device the browser is accessed from have to be connected at the same network.

  • Page 40: Help

    9.4. HELP Help is the fourth item under the System Menu. From the System Menu screen, tap Help ( The item supports users with a brief overview of the System’s main functionalities. Tap to navigate through sections and subsections for general guidelines and video support. Tap the video area to play or stop one of the three video-supported Help segments: •...

  • Page 41: Measurement & Electrode Setup Menu

    10. MEASUREMENT & ELECTRODE SETUP MENU From the Measurement screen, tap Measurement Setup icon on display to open up the choices stacked up on the left side of the screen. Measurement setup icon Setup tabs Active during system and measurement configuration. When any item in the list is accessed, a submenu will open up.
  • Page 42 Setup Table Overview Reading Parameter Rel.mV Resolution 0.01 0.001 Stability Criteria Accurate, Medium, Fast Direct, Reading Mode Direct/Autohold Temperature Parameter Rel.mV Temperature Source Manual, Automatic Temperature Unit °C, °F, K -20.0 to 120.0 °C Manual Temperature -4.0 to 248.0 °F 253.0 to 393.0 K 4.010 pH Isopotential Point...
  • Page 43 View Parameter Rel.mV Basic Basic Basic Simple GLP Graph Simple GLP Display Full GLP Table Graph Graph Table Table Alarm Parameter Rel.mV High / Low pH -2.000 to 20.000 pH – – High / Low mV -2000.0 to 2000.0 mV -20.0 to 120.0 °C High / Low -4.0 to 248.0 °F...

  • Page 44: Reading

    10.1. READING Options: Parameter, Resolution, Stability Criteria, Reading Mode Parameter Options: pH, mV, Rel. mV Option allows users to configure the meter for pH, mV (ORP), or Rel. mV (ORP with calibration) measurements. Configuration is done through direct selection. See Setup Table Overview for all options. Resolution Option allows users to select desired resolution based on selected mode.

  • Page 45: Temperature

    Reading Mode Options: Direct, Direct/Autohold Tap to select option. When Direct is selected, the measurement changes and the stability of the measurement is continuously evaluated. Unstable (blinking) or Stable status idicator are displayed above the progress bar. When Direct/Autohold is selected, a lock icon ( ) is displayed between the log and Measurement Menu icons.

  • Page 46: Calibration

    Temperature Source Menu is used to select between the physical temperature input source (Automatic) or to enter the sample temperature value manually (Manual). • For Automatic selection, a temperature probe must be plugged and placed in the measured sample. ATC will be displayed next to the temperature measurement on the pH display. The mV/Rel. mV display will indicate temperature value.
  • Page 47 • Manual When option is selected, the user manually selects the required buffer value from both Available Standard and Custom lists. First Calibration Point Point and Offset are the two options available. • With Point selected, a new buffer value can be added to an existing calibration. This prompts an automatic reevaluation of the electrode slope.

  • Page 48: View

    5. Select from available input field in the Available Custom Buffers list. 6. Tap Edit to enter buffer value at the calibration temperature value and Enter to confirm. 7. Repeat with up to 5 custom values. Once the maximum number has been reached, to add another custom value, users must delete one of the previously set values.
  • Page 49 Simple GLP In addition to data displayed when Basic option is selected, Simple GLP screen also displays: last calibration date and time, Offset value, average slope (Avg. Slope), and electrode condition (Condition). Note: If no calibration has been made, Not Calibrated is displayed. Full GLP In addition to data displayed when Simple GLP option is selected, Full GLP screen also displays: electrode symbol, used buffers trays together with calibration date, time, and temperature probe...
  • Page 50 Graph When Graph is selected, the measured value is plotted as a graph. To zoom in on a graph: 1. Select Time or parameter axis. 2. With axis selected, pinch or drag on the display. Table When Table is selected, the measured values are displayed tabulated (complete with date, time, and notes made during logging).

  • Page 51: Alarm

    10.5. ALARM Options: High pH, Low pH, High mV, Low mV, High Temperature, Low Temperature Option allows users to set the threshold limits for the measured parameters. When the parameter is enabled and the the measurement exceeds the high-limit value or drops below the low-limit value, the alarm is triggered.

  • Page 52: Logging

    10.6. LOGGING Options: Logging Type, Sampling Period (Automatic), File Name (Manual and Autohold), Sample ID (Manual and Autohold) Logging Type • Automatic Measured data is logged automatically at predefined time intervals (i.e. Sampling Period). A file name is automatically generated: pH log files start with pH.

  • Page 53: Profiles

    File Name Option available only with Manual and Autohold logging type selected. To create a file name from Logging screen: 1. Tap Create. 2. Use the on-screen keypad to enter a file name of maximum 13 characters. 3. Tap Enter on keypad to confirm. The instrument automatically ends the Manual log file name in _pH_.csv, _mV_.csv, _relmV_.csv depending on selected reading mode.
  • Page 54 To save a profile, having previously configured all other application-specific options, from the Measurement screen: 1. Tap Measurement Menu ( ) on display. 2. Tap Profiles tab. 3. Tap Save As and use the keypad to enter name for new profile. 4.

  • Page 55: Calibration

    11. CALIBRATION Interface Calibration Overview Calibration type Forward or backward navigation, sequence of steps Save and Confirm buffers, calibration procedure Standard Buffers trays, with buffer selected for transfer (rectangular outline) Custom Buffers trays Buffer management Buffers in Use Standard buffer symbol, , or when calibrating with custom buffers Electrode calibration details for buffer used (in tray)

  • Page 56: Ph Calibration

    • Manual buffer input from all available standard and custom values. Automatic Calibration With this option selected, the HI6221 automatically selects the closest buffer to the pH value being measured from all active buffers selected as Buffers in Use. Selecting Buffers in Use, Standard or Custom (navigation guidelines)
  • Page 57 3. Tap Edit. 4. Tap to select from the Available Standard Buffers or Available Custom Buffers trays. A rectangular outline highlights buffer to be transferred.
  • Page 58 5. For standard values, tap active forward arrow to move buffer in the Buffers in Use column. A small s icon ( ) on display, next to the buffer value, indicates calibration with standard buffer. Repeat with up to 5 buffers. 6.
  • Page 59 Repeat with up to 5 buffers. A small c icon on display ( ), next to the custom value, indicates calibration with custom buffers. pH Calibration Procedure using Automatic Calibration With the electrode and temperature sensor connected to the meter: 1.

  • Page 60: Relative Mv Calibration

    In addition to selecting from 8 standard options, users can define 5 custom buffers to be used for calibration. With the Semiautomatic Calibration selected, HI6221 automatically recognizes the closest buffer to the pH value being measured from all available (standard and custom) buffers.
  • Page 61 ORP values are not temperature compensated, although ORP values can change with temperature (e.g. reference electrode potential changes, sample equilibrium changes). It is important to report ORP values together with the reference electrode used and the temperature. The inert noble metal ORP surface provides an electron-exchange site with the sample (or standard) and its surface.
  • Page 62 5. Use the keypad to enter the value of the standard. 6. Tap Enter on keypad to confirm and enter the value. 7. When a stable value is reached, tap Save. The meter returns to the Rel. mV calibration setup screen. 8.

  • Page 63: Measurement

    12. MEASUREMENT Options: Direct Readings, Direct/Autohold Readings When Direct is selected, sample measurements are displayed continuously. When Direct/Autohold is selected, measurement reading is held on display when measurement stability is reached. This option removes the subjective nature of stability as a measurement that has not reached equilibrium will not be used.

  • Page 64: Direct / Autohold Readings

    • Place the electrode tip and the temperature probe approximately 4 cm (1.5”) into the sample to be measured. Allow time for the electrode to stabilize. • Unstable status indicator on the screen until measurement is stabilized. • The measured pH value is displayed on the LCD, together with GLP information and display preferences. 12.3.

  • Page 65: Error Messages

    13. ERROR MESSAGES The instrument shows clear warning messages (refer to the instrument’s on-display message area, bottom of the screen) when erroneous conditions appear, and when measured values are outside the expected range. The information below provides an explanation of the errors and warnings, and recommended action to be taken.

  • Page 66: Logging

    14. LOGGING Three logging types are available: Automatic, Manual, and Autohold. Automatic logging • Readings are logged ( ) at predefined period intervals. Sampling interval options range from 1 second to 180 minutes. • Records are continuously added to it until the session stops. •...

  • Page 67: Manual Logging

    • Tap Sample ID Prefix field to start editing. • Use the on-screen keypad to enter lot file name. • Tap Enter on keypad to save new name. • Scroll to select Prefix value. 6. Tap Home key ( ) on instrument, to reenter Measurement screen. 7.

  • Page 68: Autohold Logging

    6. Tap Home key ( ) on instrument, to return to Measurement screen. 7. From Measurement screen, tap manual logging symbol ( ) to log data. Data is logged every time symbol is tapped. 8. See option to track and log notes. Follow Automatic Logging, step 7.
  • Page 69 Note: .csv file format naming convention To help ensure that measurement—specific data files can be easily identified within a data package or after download, logged files have the measurement unit automatically included in the file name. E.g. When reading and logging in pH Parameter, automatically logged files retain pH ID at the beginning of the file.

  • Page 70: Maintenance & Conditioning

    15. MAINTENANCE & CONDITIONING Electrode Maintenance • Soak the pH bulb and reference junction in HI70300 Storage Solution for a minimum of 1 hour before attempting. • After prolonged storage or cleaning, calibration of the electrode. • After use, rinse the electrode with purified water and blot excess moisture with a lint free tissue. •...
  • Page 71 General cleaning of HI6221 The following steps outline the process to ensure users keep the benchtop clean and disinfected while limiting the risk of damage from unsuitable cleaners. • Disinfect the screen using commercially available non-ammonia glass or disinfectant cleaner.

  • Page 72: Accessories

    16. ACCESSORIES pH BUFFER CALIBRATION SOLUTIONS HI6016 Buffer solution pH 1.679 (500 mL) HI6003 Buffer solution pH 3.000 (500 mL) HI6004 Buffer solution pH 4.010 (500 mL) HI6068 Buffer solution pH 6.862 (500 mL) HI6007 Buffer solution pH 7.010 (500 mL) HI6010 Buffer solution pH 10.010 (500 mL) HI6124...
  • Page 73 ELECTRODE CLEANING SOLUTIONS HI70000P Electrode rinse sachet, 25 pcs. (20 mL) HI7061L General purpose solution (500 mL) HI7073L Protein cleaning solution (500 mL) HI7074L Inorganic substance cleaning solution (500 mL) HI7077L Oil and Fat cleaning solution (500 mL) HI8061L General purpose solution (500 mL, FDA approved bottle) HI8073L Protein cleaning solution (500 mL, FDA approved bottle) HI8077L...
  • Page 74 HI1053B Glass body, triple ceramic, conical shape, refillable, combination electrode Ideally suited for emulsions. HI1083B Glass body, micro, viscolene, non refillable, combination electrode Application: biotechnology, micro titration HI1131B Glass body, refillable, double junction, combination electrode Application: general purpose...
  • Page 75 HI1330B Glass body, semimicro, single junction, refillable, combination electrode Application: laboratory, vials HI1331B Glass body, semimicro, single junction, refillable, combination electrode Ideally suited for flasks. HI1230B Plastic body (PEI), double junction, gel filled, combination electrode Application: general, field...
  • Page 76 HI2031B Glass body, semimicro, conical, single junction, refillable, combination electrode Application: semisolids HI1332B Plastic body (PEI), double junction, refillable, combination electrode Application: general purpose HI1413B Glass body, single junction, flat tip, viscolene, non refillable, combination electrode Application: surface measurement FC100B Plastic body (PVDF), double junction, refillable, combination electrode Application: general purpose for food industry...
  • Page 77 FC200B Plastic body (PVDF), single junction, conical, viscolene, non refillable, combination electrode Application: meat and cheese FC210B Glass body, double junction, conical, viscolene, non refillable, combination electrode Application: milk and yogurt FC220B Glass body, triple ceramic, single junction, refillable, combination electrode Application: food processing FC911B Plastic body (PVDF), double junction, refillable with built-in amplifier, combination electrode...
  • Page 78 HI7855/1, 1 m (3.3’) long CONNECT TO CONNECT TO HI7855/3, 3 m (9.9’) long SCREW TYPE ELECTRODES METER BNC SOCKET Connector and 3.0 mm (0.12”) cable with BNC Please refer to the Hanna Instruments general catalog for more electrodes with screw-type or BNC connectors.

  • Page 79: Certification

    If service is required, contact your local Hanna Instruments office. If under warranty, report the model number, date of purchase, serial number (see engraved on the back of the meter) and the nature of the problem.

  • Page 80: Regulatory Notices For The Wi-Fi Module

    REGULATORY NOTICES FOR THE WI-FI MODULE United States (FCC) FCC ID: 2ADHKATWINC1500. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
  • Page 83 Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 84 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN6221 Printed in ROMANIA...

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